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Citations for Millipore Sigma :
601 - 650 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: pLKO.1-puro lentiviral vectors expressing shRNAs targeting FASN (shFASN_1: NM_004104.x-1753s1c1 and shFASN_2: NM_004104.x-3120s1c1) were purchased from Sigma-Aldrich. An mCherry-LC3B lentiviral vector was kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: ... or non-targeting control in the pLKO.1 vector were obtained from the Mission shRNA Library (Sigma).
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bioRxiv - Cell Biology 2021Quote: NFIC expression was interfered in 266-6 cells using Mission shRNA lentiviral constructs purchased from Sigma-Aldrich. Nfic sh1 [TRCN0000374154 targeting ACAGACAGCCTCCACCTACTT) ...
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bioRxiv - Cell Biology 2021Quote: ... The AURKA-specific shRNA (SHCLNG-NM_003600) and a non-targeting control (SHC002) were purchased from Sigma-Aldrich. Plasmids and shRNAs were transfected by the calcium phosphate method or with Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... as well as pLKO.1 (50) containing either non-targeting or IFIT1 shRNA (Sigma, TRC1, Clone: TRCN0000158439). Huh7 cells were transduced with the indicated shRNA expressing lentivirus then selected with 2 μg/ml puromycin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... All lentiviral vectors (pLKO.1) expressing shRNAs used for knockdown of host proteins were purchased from Sigma.
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bioRxiv - Cell Biology 2019Quote: MISSION TRC shRNA lentiviral library containing 80’000 lentiviral clones targeting 15’000 genes was purchased from Sigma-Aldrich. Cells (3,000 per well ...
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bioRxiv - Molecular Biology 2021Quote: All lentivirus-based shRNA clones used for making the viral transduction particles were purchased from Sigma-Aldrich. pLKO.1-Puro vector targeting human PPARA or non-target vector as a control was used ...
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bioRxiv - Microbiology 2021Quote: ... ICAM-1kd PLB985 cells were generated by lentiviral transduction with ICAM-1 specific shRNAs from Sigma (TRCN0000372478). EPCRkd PLB985 cells were generated by lentiviral transduction with EPCR specific shRNAs from Sigma (TRCN0000300553) ...
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bioRxiv - Cancer Biology 2022Quote: Expression of either CAPN1 or CAPN2 was knocked down in U251N using shRNA purchased from Sigma-Aldrich (CAPN1 ...
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bioRxiv - Cell Biology 2022Quote: shRNAs against APPL1 and EEA1 were selected from de Broad Institute GPP database and purchased from Sigma (MISSION TRC shRNAs purified plasmid DNA) ...
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bioRxiv - Cancer Biology 2022Quote: pLKO.1-puro lentiviral vectors expressing shRNAs targeting HSP8A (shHSPA8_1: NM_006597.3-2040s21c1) and HSP90AA1 (NM_005348.x-1505s1c1) were purchased from Sigma-Aldrich. These vectors contain a puromycin antibiotic resistance gene for selection of transduced mammalian cells ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNAs that stably integrated into mouse cells were chosen through selection with 10 μg/ml puromycin (Sigma). For the generation of Nat10-overexpressing cells ...
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bioRxiv - Cell Biology 2024Quote: Stable MMP9 knockdown cell lines were generated using two independent shRNA sequences (CCACAACATCACCTATTGGAT and CAGTTTCCATTCATCTTCCAA; Sigma Aldrich). The transduced cells were selected using puromycin (Catalogue number CMS8861 ...
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bioRxiv - Cancer Biology 2023Quote: ... we transduced MCF7 and HS5 cells with shRNA against CCDC88A (clone TRCN0000129915, Millipore Sigma, Burlington, MA, USA), preparing lentiviruses as described previously (87) ...
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bioRxiv - Cancer Biology 2023Quote: An shRNA library targeting 284 pancreatic cancer related genes was generated using the pLKO vector backbone (Sigma). Each gene was targeted with 3-4 shRNA producing a library size of 1013 shRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... As a control we used a shRNA against Luciferase (cat# SHC007, Sigma-Aldrich, Saint Louis, MO, USA). The following sequences were used as a shRNA target shRNA-Luciferase ...
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bioRxiv - Cancer Biology 2023Quote: ... The LDHA shRNA vector (TRCN0000164922) was validated for 96% knockdown in HEK293T cells (Sigma, St. Louis, MO). Both vectors were packaged into lentiviral particles prior to transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentiviral shRNA constructs in the pLKO.1-puro vector were purchased as glycerol stocks from Millipore Sigma. For shADAR1 ...
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bioRxiv - Cancer Biology 2024Quote: ... or control cells expressing non-mammalian targeting control shRNA (pLKO.1-Puro_shCtrl; MISSION® SHC002, Sigma-Aldrich) were generated using HEK293T cells ...
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
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bioRxiv - Immunology 2020Quote: ... PD1kd 4T1 and AT3 cells were generated by lentiviral transduction with PD1 specific shRNAs from Sigma (TRCN0000097670-74). Control cells were transduced an empty vector (pLKO) ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles for shRNA knockdown of NTRK2 were produced following transfection of the lentiviral packaging vectors (pΔ8.9 and VSV-g) and either the PIK3CA shRNA vector (TRCN0000197207; Sigma) or the control shRNA vector (SHCOO2 ...
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bioRxiv - Molecular Biology 2019Quote: CYLD knockdown was achieved using a stably expressed lentiviral pLKO.1 puro mouse CYLD shRNA construct (Sigma, MC5) and nontarget shRNA (Sigma ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: shRNA Endo180 knock-down clones from 3T3 and CAFs were generated using lentiviral particles (Sigma, Supplementary Table 3). For siRNA knockdown ...
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bioRxiv - Neuroscience 2021Quote: Ten days post induction of shRNA against TDP-43 with 1 μg/ml doxycyline hyclate (Sigma D9891-1G), SH-SY5Y cells were treated either with 100 μM cycloheximide (CHX ...
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bioRxiv - Neuroscience 2021Quote: ... For induction of shRNA against TDP-43 cells were treated with 5 μg/mL Doxycyline Hyclate (Sigma D9891). After 3 days media was replaced with Neurobasal (Thermo ...
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bioRxiv - Cancer Biology 2021Quote: ... The shVCP (TRC0000004249) and shHER3 (TRCN0000218392) pLKO.1 constructs were from the Mission shRNA collection from Sigma-Aldrich. Lentivirus particles expressing shRNA against the gene of interest were generated by co-transfection with the VSV-G packaging and CDNL envelope plasmids (courtesy of Biplab Dasgupta ...
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bioRxiv - Cancer Biology 2022Quote: For lentiviral production, shRNA clones targeting CAD (TRCN0000045910, TRCN0000045908) and DHODH (TRCN0000025868, TRCN0000025839) were purchased from Sigma-Aldrich, doxycycline inducible shRNA targeting DPYD (V2THS_84048 ...
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bioRxiv - Cancer Biology 2020Quote: Single stranded cDNA sequences of mouse Id1 and Id3 shRNAs were purchased from Sigma-Aldrich (Lismore, NSW, Australia). The Id1 shRNA sequence which targets 5’-GGGACCTGCAGCTGGAGCTGAA-3’ has been validated earlier (Gao et al. ...
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bioRxiv - Immunology 2020Quote: ... Huh7 cells were transduced with the indicated shRNA expressing lentivirus then selected with 2 μg/ml puromycin (Sigma). After selection ...
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bioRxiv - Cancer Biology 2019Quote: ... and lentiviral vectors: non-targeting control (PLK0.1) or p75NTR-targeting MISSION shRNA constructs (Sigma-Aldrich, TRCN0000058153 and TRCN0000058153). The medium was changed 8 hours post-transfection ...
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bioRxiv - Molecular Biology 2019Quote: HCT116 cells were transfected with pLKO.1 vector expressing either an eIF6 targeted shRNA (CCGGGTGCATCCCAAGACTTCAATTCTCGAGAATTGAAGTCTTGGGATGCACTTTTT G, Sigma - TRC327700) or a control non-targeting shRNA (Sigma ...
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bioRxiv - Neuroscience 2024Quote: For induction of shRNA against TDP-43 cells were treated with the following amounts of doxycycline hyclate (Sigma), and collected after 10 days:
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bioRxiv - Cancer Biology 2023Quote: Four Lenti-pLKO.1-puro shRNA vectors that specifically targeted COL7A1 mRNA sequences were chemically synthesized (Sigma Aldrich) and included in viral particles:
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bioRxiv - Biochemistry 2023Quote: ... HEK 293t cells were infected with commercially available Lentiviral particles for hTRMT2A KD and scrambled control cell line (MISSION® shRNA Lentviral Transduction Particles: scrambled control WT = SHC002V, KD1 = NM_182984.2-856s1c1, KD2 = NM_182984.2-1574s1c1; Sigma-Aldrich). Cells with stable integration of shRNA were selected as puromycin-resistant colonies ...
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bioRxiv - Cancer Biology 2023Quote: METTL3 (sh1 and sh2) and Control shRNA in pLKO.1-TRC cloning vector were purchased from Sigma-Aldrich to generate stable METTL3-KD cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Human haemoglobin (Sigma) was used for creation of standard curve.
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bioRxiv - Microbiology 2021Quote: ... human insulin (Sigma), 10’000 units/ml of penicillin and 10’000 µg/ml streptomycin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... human HDL (Millipore), human LDLs (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... human LDLs (Millipore) or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human hemoglobin (Sigma) was dissolved in PBS to 10 mg/mL or 1 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... Human DPP4 (Sigma) was used as a control in PBS at 50 ng/μL (0.585 μM) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...