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Citations for Millipore Sigma :
851 - 900 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... A human IgG (Sigma #I2511) standard curve (ten three-fold serial dilutions starting at 20 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... 2% human serum (Sigma-Aldrich), 1% non-essential amino acids (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... human insulin (I2643-25mg, Sigma) and monothioglycerol (M1753 ...
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bioRxiv - Cell Biology 2024Quote: ... 850 nM human insulin (Sigma), 500 nM IBMX (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... 850 nM human insulin (Sigma), 500 nM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant human PDE8A (Sigma SRP0273) was incubated with 100 ng FAK tyrosine kinase (Promega #V1971 ...
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bioRxiv - Molecular Biology 2024Quote: Human serum (Sigma-Aldrich, H4522) was thawed on ice and centrifuged at 18,000 ×g for 10 min at 4 °C to remove lipids ...
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bioRxiv - Immunology 2024Quote: ... 10% human serum (Sigma-Aldrich), 100 U/ml Penicillin (Cellgro Technologies LLC) ...
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bioRxiv - Bioengineering 2024Quote: ... Human thrombin (Sigma, #T6884-100UN) was reconstituted in sterile 0.9% saline supplemented with human serum albumin (0.1% w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... thrombin (from human plasma, Sigma) and aprotinin (from bovine lung ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Sigma), 500 IU/mL of IL-2 (Proleukin S ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were isolated (GenElute Plasmid Miniprep Kit, Sigma Aldrich, St. Louis, Missouri, USA) and sequenced with suitable primers (Eurofins Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was isolated with a GenElute™ Plasmid Miniprep Kit (Sigma Aldrich) according to the provided protocol.
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was obtained using the GenElute Plasmid Miniprep Kit (Ref. PLN350, Sigma) also including a lysis step with lysozyme (2.5 mg/ml ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... SiRNA constructs with the siRNA cloned in the pLKO.1-Puro vector were obtained from the MISSION shRNA library (Sigma product SHGLY). The specific siRNA sequences used are:
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bioRxiv - Cancer Biology 2020Quote: ... SASI_Hs01_00221653) and shRNAs targeting TRIM27 (SHCLNG-NM_006510; TRCN0000280319 and TRCN0000011021) or a non-targeting control were purchased from Sigma (St. Louis, MO).
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bioRxiv - Cancer Biology 2021Quote: ... Knockdown of EWS-FLI1 in shSK-E17T cells was performed by shRNA induction by addition of 1 μg/ml Doxycycline (Sigma-Aldrich, USA) for 7 days pre-transplantation.
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bioRxiv - Microbiology 2020Quote: ... Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3) or GFP gene in the presence of polybrene (5 µg/ml; Sigma-Aldrich, Poland). Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... TRCN0000148694) and a control scrambled shRNA pLKO.1 clone (SHC-002) were performed using MISSION Lentiviral Packaging Mix (Sigma-Aldrich, Diegem, Belgium) and FuGENE HD (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... laboratory protocol “Lentivirus production of shRNA or ORF-pLX clones,” and single clones were isolated following puromycin selection (2 μg/mL; Sigma-Aldrich #P8833). RNA-seq data were aligned with STAR to mouse reference genome mm10 (GRCm38) ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2024Quote: ... and human apo-transferrin (P02787) isolated from human plasma were purchased from Sigma Aldrich (E0518), Invitrogen (RP-43132) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... we transfected lentiviral plasmids with packaging plasmids in 293T cells using polyethylenimine (Sigma, 408727), harvested supernatants containing viral particles 48 and 72 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were prepared using the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (NA0410; Sigma). Silica fines in the final product were carefully eliminated by additional centrifugation at 12,000 g for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with the Gene Elute Plasmid Miniprep kit (Sigma, St. Louis, USA) and sent for sequencing to two different companies (EuroFins Genomics ...
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bioRxiv - Molecular Biology 2019Quote: ... The plasmid pET-28a (+) (Novagen) carries an N-terminal His-Tag/thrombin/T7 configuration ...
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bioRxiv - Cancer Biology 2020Quote: ... ShScr and shIDO plasmids (Sigma)[35] were electroporated into ST strains using a BTX electroporator (1mm gap cuvettes ...
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bioRxiv - Biochemistry 2020Quote: ... carrying the pRARE2 plasmid (Novagen) and initially purified using the same protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pRSFDuet-1 plasmids (Novagen) encoding SARS-CoV-2 nsp14 or nsp10 engineered with an N-terminal His-SUMO tag were prepared as follows ...
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bioRxiv - Microbiology 2021Quote: ... plasmids and primers (Sigma, Singapore) used in this study are listed in S6 and S7 Tables ...
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bioRxiv - Synthetic Biology 2023Quote: The pET-11a plasmid (Novagen) was used as the genetic vector to host all three components constituting our genetic circuit ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids were purchased from Sigma.
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bioRxiv - Genetics 2023Quote: ... harboring the pRARE plasmid (Novagen). Super Broth media (3 L ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid pET-19b (Novagen) was double digested using NcoI and BamHI and Gibson assembly performed to insert a gene construct into the plasmid ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Immunology 2021Quote: ... Expression of PTSN was silenced in U2-OS and 293A cells by lentiviral transduction of a pLKO1 shRNA against both β- and α-PTSN (TRCN0000282572, Sigma-Aldrich). pLKO1 shRNA (SHC016V ...
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bioRxiv - Microbiology 2019Quote: ... The Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible lentiviral backbone pLKO-puro-IPTG_3xLacO harboring the non-mammalian shRNA control was purchased (Mission™SHC332; Sigma Aldrich, Buchs, Switzerland). The puromycin resistance gene was replaced via restriction digestion cloning using a PCR-amplified fluorescent mCherry marker containing overhangs with compatible restriction sites to generate the pLKO-mCherry_IPTG_3xLacO lentiviral backbone.
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bioRxiv - Cancer Biology 2019Quote: ... for primary cells miR-E shRNA viruses were concentrated to increase transduction efficiency using Centriprep Centrifugal filter YM-50 (Millipore, Billerica, MA, USA).
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bioRxiv - Physiology 2021Quote: ... The shRNA-containing vector was co-transfected with the pE-ampho vector into HEK293T cells using GeneJuice transfection reagent (Merck Millipore, Burlington, MA, USA). Supernatants containing viral particles were collected 48 h after the transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... HT-29/19A and HCT-116 cells were transduced with lentivirus expressing short-hairpin RNAs (shRNAs) targeting fascin1 (Sigma-Aldrich, St. Louis, MO). For the rescue experiments ...
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bioRxiv - Cell Biology 2023Quote: U2Os-AP2-GFP cells were transduced with lentiviral media expressing respective shRNAs in the presence of Polybren 8 μg/ml (Sigma-Aldrich, TR-1003) for 5 hours ...
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bioRxiv - Immunology 2022Quote: Lentiviral plasmids encoding shRNA for gene knockdown for PRDM1-L or open reading frame of overexpression for PRDM1-S and PRDM1-L were obtained from Sigma-Aldrich (MISSION shRNA) and Horizon Discovery Biosciences (Precision LentiORF) ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...