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Citations for Millipore Sigma :
6351 - 6400 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: Tissue from the LV of 3 sedentary and 3 exercised mice of each sex was fixed in RNAlaterTM (Millipore Sigma, MFCD03453003), and RNA sequencing was performed by Genewiz from Azenta Life Sciences ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... Slides were incubated for 3 minutes in 3% acetic acid and then for 20 minutes in Alcian blue solution (Sigma Aldrich) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... a shRNA sequence based on 5’-GCCTAAATGGTCAAGGAGATA-3’ as the sense nucleotide corresponding to the 3’UTR of mouse mDia1 (NM_007858.4; Sigma-Aldrich, Cat# TRCN0000108685) was designed as an oligonucleotide with overhangs (s ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were processed to 2’-2’-thio-diethanol (TDE, Sigma, 166782) for clearing and mounting ...
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bioRxiv - Immunology 2022Quote: ... 5 μM oligomycin and 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich) were injected to each well after 18 ...
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bioRxiv - Immunology 2021Quote: ... 2-Deoxy-D-glucose (2-DG) was obtained from Sigma Aldrich (D8375) and used at 0.32 mM ...
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bioRxiv - Genomics 2020Quote: ... Substrate solution (2 mM ABTS, Sigma A9941; 2 mM hydrogen peroxide, Sigma H1009 ...
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bioRxiv - Cell Biology 2021Quote: ... The last injection was supplemented with 2-deoxyglucose (2-DG, Sigma, D8375) to obtain the ECAR background value due to nonglycolytic acidification ...
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bioRxiv - Immunology 2021Quote: ... For the last 2 h 2 µg/ mL Brefeldin A (Sigma-Aldrich) was added ...
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bioRxiv - Immunology 2021Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: ... cells were treated with 2-deoxyglucose (2-DG) 100 nM (Sigma, #D8375).
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bioRxiv - Immunology 2020Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µM epoxomicin and 50 µM (Z-LL)2-ketone (Merck Millipore) were added from stock solutions in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1µM Oligomycin A and 50mM of 2-deoxyglucose (2-DG) (Sigma, D3179). For mitostress tests ...
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bioRxiv - Developmental Biology 2024Quote: ... dissolved in water and 2-2’ bipyridyl (BP) (Sigma‒Aldrich Cat# D216305) dissolved in DMSO were added to the NGM before pouring to final concentrations of 4 mmol/L ...
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bioRxiv - Bioengineering 2024Quote: ... methyl triflate and 2-ethyl-2-oxazoline were obtained from Sigma Aldrich and dried over CaH ...
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bioRxiv - Biochemistry 2024Quote: ... oligomycin and 2-deoxy-D-glucose (2-DG, Sigma-Aldrich, D8375-1G)) at final well concentrations of 10 mM ...
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Multi-scale Assessment of Brain Blood Volume and Perfusion in the APP/PS1 Mouse Model of AmyloidosisbioRxiv - Neuroscience 2022Quote: ... the CUBIC solution 1 was washed away with 6 PBS washes over the course of 6 hours and the brain tissue was then incubated overnight at RT with a 1:1 mix of PBS and CUBIC solution 2 (50 % sucrose, 25 % urea and 10 % TEA [Triethylamine, Sigma-Aldrich] weight/weight in MiliQ). The next morning ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301, Goat α-mouse HRP 1:1000– Sigma Aldrich/GERPN4401), revealed using ECL reagent (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... washed in water until the stain turned blue approximately 2 mins and then counter stained with 1% Eosin (Sigma-Aldrich, Cat. no. 17372-87-1) for 30 Sec ...
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bioRxiv - Genetics 2021Quote: ... 2 mM EDTA and 1% Triton X-100) with 20 mg/mL lysozyme from chicken egg (Sigma, L4919) using a tissue grinder (BMS BC-G10 ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... t Phospho-Myosin Light Chain 2 (Ser19) antibody (3671; Cell Signalling) 1:50 and Recoverin antibody (AB5585; Millipore) 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Sigma) (sigma) ...
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bioRxiv - Immunology 2022Quote: ... All wells were treated for 1 hour with 10 µM 2’,7’-dichlorofluorescin diacetate (DCF-DA, Sigma, D6883) in Ringer buffer (155 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min and blocked for at least 2 h with a blocking buffer containing 1% BSA (Sigma), 3% donkey serum (Millipore) ...
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bioRxiv - Evolutionary Biology 2020Quote: Adult male frogs (n = 2) were anesthetized with 1% MS-222 (tricaine, Sigma-Aldrich, St. Louis, MO, USA) and sacrificed by decapitation ...
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bioRxiv - Cell Biology 2021Quote: ... topped up to 140 ug/ul for the final 2 h and then DNA was extracted with 20 ml Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Sigma), precipitated with ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2020Quote: ... HIV-GFP produced with p8.91 or p8.2 used in Figure 1 were DNase treated for 2 hours at 37°C (DNaseI, Sigma) prior to ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Neuroscience 2020Quote: ... A borosilicated glass micropipette was filled with NES and 2 mM Glutamate or 1 mM GABA (Sigma Aldrich), and moved via a micromanipulator MP-225 (Sutter Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... Filtered cells were immediately seeded in 96 well plates coated with 1% Poly (2-hydroxyethyl methacrylate; Sigma Aldrich) at a density of 200 or 50 cells per well ...
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bioRxiv - Cell Biology 2021Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW: 114,17; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543 ...
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bioRxiv - Biochemistry 2022Quote: ... Fatty acids were extracted using a chloroform-methanol mix (2:1; Thermo-Fisher) in 0.01% butylated hydroxytoluene (Sigma) prior to centrifugation at 200 g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: A375 cells in 100 mm dishes were challenged by 2 mM of octanoate-1-13C (Sigma Aldrich #296457) for 6 or 20 hrs ...
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bioRxiv - Cell Biology 2022Quote: Evaporated lipids were resuspended in 10µL 2:1 chloroform/methanol and spotted on dehydrated silica plates (EMD Millipore; dehydrated by baking for 30 minutes at 100°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with secondary antibodies for 2 h: Alexa Fluor 488 goat anti-mouse IgG (1:400, Millipore), or Fluor 594 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... The quenched samples were concentrated to 60 μl by centrifugation at 20,000xg for 2 min (Sigma 1-16K). Due to the linear nature of DNA ...
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bioRxiv - Neuroscience 2022Quote: ... samples were stained with the fluorescent nucleic acid marker 4,6-diamino-2-fenilindol (DAPI 1:3000; Sigma-Aldrich), and mounted with a Fluorescent Mounting Medium (DAKO ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 2:1 ratio of beads to reaction volume with an additional 1.8x supplementation of isopropanol (Sigma).
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bioRxiv - Genetics 2021Quote: ... the resin with the immobilized MBP-Rif2 variants was first washed 4 times with 1 ml wash buffer containing 1M NaCl (50 mM Tris-HCl pH 7.5, 1 M NaCl, 0.2% NP40, 2 mM EDTA, Sigma protease inhibitory cocktail P8340 1:1000) ...
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bioRxiv - Cancer Biology 2021Quote: ... universal MISSION® siRNA Universal Negative Control #1 (cat. SIC001) and #2 (cat. SIC002) were from Millipore Sigma. We used the DharmaFECT 4 Transfection Reagent (Thermo Scientific ...