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Citations for Millipore Sigma :
6251 - 6300 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Sections were washed 3×15min in PBS 0,01% Triton X-100 (PBST) and nuclei stained with DAPI (Sigma-Aldrich, #D9542, 1:10000) for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then blocked in 3% BSA in PBS containing 0.2% Tween 20 before staining with rat anti-HA mAb 3F10 (Sigma, diluted 1:1,000) primary antibody in blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μg of pSC2G-CFTR23 plasmid was co-transfected with 1 μM single-stranded DNA repair template (synthesized by Sigma, Table 3) comprising the CFTR-W1282X and silent protospacer adjacent motif (PAM ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were next washed four times for 3 min in MilliQ water at room temperature and incubated in a 1% solution of thiocarbohydrazide (Sigma-Aldrich, USA) for 20 min at 60 °C ...
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bioRxiv - Genomics 2020Quote: ... 3 million CD4+ T cells were incubated in the presence of 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB, Sigma-Aldrich, Cat: D1916) at a final concentration of 65μM or Triptolide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Wet chemical etching solution for Au: 20 mM Fe(NO3)3·9 H2O (Fluka, Switzerland), 30 mM thiourea (Fluka, Switzerland) and 1 mM HCl (Sigma-Aldrich, USA) dissolved in ultrapure water saturated with octanol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... and 8 DAP and cleared in a chloral hydrate solution (chloral hydrate, Sigma C8383/Glycerol/Water, 8/1/3 w/v/v) for embryo phenotyping ...
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bioRxiv - Cell Biology 2020Quote: ... The liver tissue was cut into 3-4 mm pieces and incubated with 1 mM EGTA (Sigma-Aldrich, Cat. no. E0396-10G) in 1 x DBPS (Gibco™ ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were cultured in N2B27 containing either LIF and BMP4 (10 ng/ml, Stemgent 03-0007) or CHIR99021 (3 μM, Stemgent 04-0004-02) and PD0325901 (1 μM, Millipore Sigma 444968) respectively.
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bioRxiv - Bioengineering 2022Quote: ... were functionalized with O2 plasma (30 W / 30 s) followed by incubation in 1% v/v (3-Aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich 440140) in water for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then treated overnight in 1 volume of 100 % methanol and l/3 volumes of 100 % dichloromethane (DCM; Sigma-Aldrich; 270997). On the next day ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were typically seeded at a density of 1-3 × 105 cells/cm2 on dishes coated with poly-L-lysine (Sigma-Aldrich, P1524). Neuronal cultures were treated overnight in 1 μM tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2022Quote: ... in addition to 10% FBS and 1% PS we supplemented the medium with 3 μg/ml puromycin (Sigma Aldrich, cat. no. P8833). For the experiments that involved transfection and electrophysiological recordings ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Cancer Biology 2020Quote: ... For dissociation to single cells the tissue was minced using scissors and incubated with enzymatic digestion solution containing 3 mg ml-1 collagenase A (Sigma Aldrich, 11088793001) and 70 unit ml-1 DNase in RPMI 1640 (Biological industries ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Genomics 2021Quote: ... The dried samples were reconstituted using a buffer solution (0.1% formic acid: acetonitrile = 97:3) and was then filtered through a 0.45 μm centrifuge filter (Millipore; 1 min, 13,000 rpm). The supernatant was then transferred to a 96-well collection plate ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Cell Biology 2020Quote: ... This basal medium was supplemented with 2i-LIF (3 µM CHIR99021, Sigma-Aldrich, cat. no. SML1046 and 1 µM PD0325901 Sigma-Aldrich, cat ...
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bioRxiv - Biochemistry 2021Quote: ... Dried supernatant was dissolved in 1 ml of 5 mM ammonium bicarbonate and purified on a 3 ml porous graphitic carbon column (EnviCarb; Sigma Aldrich – Supelco) equilibrated with 2 ml of 80 % acetonitrile solution with 0.1 % TFA and 2 ml of water ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1:100 dilution of GlutaMax and penicillin/streptomycin and cells were treated (n = 3 per group) with compounds and tBHQ (Sigma 11294-1) for 24 hr ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were spin-infected at a 0.3 multiplicity of infection in the presence of DMEM (10% FBS, 1% L-Glutamine) and hexadimethrine bromide (8ug/ml) (Sigma-Aldrich, 107689) at 2000 rpm ...
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bioRxiv - Immunology 2022Quote: ... Wells were then washed three times with 0.05% Tween 20 in PBS and blocked for 1 hour at room temperature with 100 μL of 3% globulin free BSA (Sigma-Aldrich, A7030) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... both HeLa cells and cortical neurons were permeabilized after PFA fixation with 100% methanol chilled at −20℃ for 20 minutes followed by 1 h blocking with PBS containing 3% BSA (Sigma-Aldrich; A9418). Cells were stained with antibodies against SIN capsid ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 times with D-PBSX before incubation with the corresponding secondary antibodies (all diluted 1:500) and DAPI (Sigma, #32670-25mg) for 1 h (RT) ...
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bioRxiv - Physiology 2024Quote: ... The rate of pH change was measured every 13 minutes for a 3-minute interval before sequential challenge with 1) 20mM glucose (Sigma Aldrich D9434), 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 3 hours with 1 µg/mL lipopolysaccharides (LPS) from Escherichia coli O111:B4 (Sigma-Aldrich #L2630; Lot #000110081) in 0.25%(w/v ...
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bioRxiv - Neuroscience 2024Quote: ... with 100 mM NaCl and 1 mM DTT and concentrated to 20-60 mg/mL by ultrafiltration using a 3-kDa cutoff filter (Millipore-Sigma, Burlington, MA).
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 h in serum- free RPMI-1640 (with 10,000 U/mL Penicillin, 10 mg/mL Streptomycin, 1:100) (Sigma-Aldrich, Sydney, Australia) at a density of 0.2 × 106 cells/cm2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with PBS and DNA content were visualized by DAPI (1 μM; Cat# D9542, Millipore Sigma, I h) staining at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were amputated to induce Erk activation and then dissociated at 3 hpa in CMF supplemented with a 1:10 dilution of Liberase TH (Millipore Sigma, 5401135001) into single cells (Witchley et al. ...
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bioRxiv - Cell Biology 2023Quote: ... with secondary antibody in PBST containing goat serum, washed (3 × 5 min), stained (10 min, 20°C) with DAPI (1 µg/ml in PBS; Sigma, Cat# D9542) and washed (5 min ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours post-knockdown and induction coverslips were fixed in -20oC methanol for 3 minutes and stained for ms-gamma tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig CENP-C 1:25 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... Cover glasses were then activated by silanization with an ethanol solution supplemented with 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and stained for 15 minutes in Alcian Blue solution (1% Alcian Blue in 3% solution of acetic acid) (Cat. # A5268, Sigma Aldrich, UK). Slides were then washed in running tap water for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 1xPBS + 0.5% Tritton X-100 + 3% Bovine Serum Albumin before adding the primary antibody (HPA036814, Sigma-Aldrich – 1/1000) for 48h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted to a starting OD600 of 0.01 in 3 mL of BHIS supplemented with increasing concentrations of 1) bile salt mixture (50% cholate and 50% deoxycholate; Sigma-Aldrich B8756), 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
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bioRxiv - Plant Biology 2024Quote: ... and combined with 0.2 mL of a 3 mg L-1 TAG C15:0 solution (Tripentadecanoin, reference T4257, Sigma-Aldrich™, USA) in anhydrous hexane 95 % (296090 ...
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bioRxiv - Molecular Biology 2020Quote: ... One milligram of peptide was dissolved in 20 ul of 10% trifluoroacetic acid (TFA, Sigma-Aldrich), in an H218O base (95-98% 18O ...
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bioRxiv - Microbiology 2020Quote: ... One μL of a 4 mM of 1,6-diphenyl-1,3,5-hexatriene (DPH) stock solution (Sigma-Aldrich) in tetrahydrofuran was added to 1 mL aliquot of the resuspended cultures and incubated in the dark for 30 min at 37 °C to allow the probe to incorporate into the cytoplasmic membrane ...