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Citations for Millipore Sigma :
6301 - 6350 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... by constant pressure and in the presence of 1 mM 3-AT (Sigma). The injected zygotes were cultured at 16C for Sp ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated using a 3 kDa cutoff spin concentrator (Millipore Sigma UFC9003) to a volume of 2 mL ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated in secondary antibodies (Supplemental Table 3) and DAPI (10g/ml, Sigma,) diluted in 10% NDS in 0.1% Triton-PBS for 90 minutes at r.t ...
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bioRxiv - Biochemistry 2019Quote: ... pH 8.0) and eluted with Strep buffer containing 3 mM Desthiobiotin (Sigma-Aldrich). Both 10-His tag and Twin-strep tag were subsequently cleaved by PreScission protease (P3C ...
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bioRxiv - Neuroscience 2019Quote: ... 1:100 mouse anti-GFP (referred as anti-GRASP, Sigma #G6539, ref:3), 1:500 rat anti-N-Cadherin (Developmental Studies Hybridoma Bank ...
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bioRxiv - Cell Biology 2019Quote: ... Dynamin TKO was induced by the application of 3 µM Hydroxytamoxifen (OHT, Sigma) for 2 days ...
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bioRxiv - Microbiology 2019Quote: ... the cells were incubated with a rabbit anti-Gal-3 antibody (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3 ml of selective EZ containing 0.85 g/l Pluronic F-127 (Sigma) were inoculated with the overnight preculture in a 1:100 ratio and grown for 3-4 h at 37° C ...
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bioRxiv - Plant Biology 2019Quote: ... the various lines were homogenized using 3 mm zirconium glass beads (Sigma-Aldrich), and genomic DNA (gDNA ...
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bioRxiv - Genomics 2019Quote: ... The liver was digested by perfusion with Solution 3 (collagenase buffer, Sigma-Aldrich), flow rate 100-400mL/min ...
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bioRxiv - Physiology 2019Quote: ... and 30µl of thrombin (3 units in 30µl of CaCl2 solution, Sigma-Aldrich). Gel polymerization occurs rapidly at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... DTT and 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine were purchased from Sigma-Aldrich, St ...
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bioRxiv - Synthetic Biology 2020Quote: ... followed by plating on SP4 agar containing 3 µg/mL of puromycin (Sigma). Colonies appeared after 3 to 4 days at 37 °C.
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were treated with 3% H2O2 (v/v in 1xPBS; Millipore Sigma, Canada) to eliminate endogenous peroxidase activity and dehydrated overnight in 70% ethanol ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were washed 3 times and maintained in XF-DMEM (Sigma-Aldrich) supplemented with 1 mM sodium pyruvate and 17.5 mM glucose ...
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bioRxiv - Pathology 2020Quote: ... followed by a permeabilization step with 3% Triton™ X-100 (Sigma Aldrich) for an additional 5 min in the blocking buffer ...
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bioRxiv - Microbiology 2020Quote: The magnetic extraction set-up consisted of three 3 mL cuvettes (Sigma Aldrich) and a neodymium magnet (60×10×3 mm ...
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bioRxiv - Biochemistry 2019Quote: ... coated for 3 hr at room temperature with monoclonal anti-FLAG (Sigma-Aldrich) and blocked for 2 hr at room temperature with 3% BSA and allowed to bind overnight at 4 °C with shaking ...
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bioRxiv - Neuroscience 2020Quote: ... 1.2 μL of MgCl2 (25 mM, Sigma-Aldrich, #M8266; CAS 7786-30-3), 2 μL of genomic DNA ...
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bioRxiv - Microbiology 2021Quote: ... 3 dpf MD zebrafish were soaked in 25 μg/mL lipoteichoic acid (Sigma) or 25 μg/mL peptidoglycan (Sigma).
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bioRxiv - Physiology 2020Quote: ... blocked with PBST containing 3 % normal goat serum (blockPBST; Sigma-Aldrich, MO, USA) for 1 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aggregates were incubated with primary antibodies in blocking buffer (3% BSA (Sigma, A9647) and 0.3% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and 1µl of each primer (10µm, 5’: FAATGGCAGAGTGGGGTTGGG, 3’: CGGCAAACGGACAGAAGCATT, Sigma-Aldrich, USA).
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bioRxiv - Biophysics 2020Quote: ... the larvae were anesthetized with tricaine (3-amino benzoic acidethylester, Sigma Aldrich, MO) and immobilized in 1% low-melting-point agarose inside FEP (Fluorinated Ethylene Propylene ...
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bioRxiv - Microbiology 2019Quote: ... 1/200 (v/v) each phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), and 1/100 (v/v ...
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bioRxiv - Microbiology 2020Quote: ... The grids were washed 3 times with 1% bovine serum albumin (BSA; Sigma) in DPBS and blocked in 0.1% gelatin (Aurion ...
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bioRxiv - Microbiology 2021Quote: ... we pumped ∼3-6 liters of fluid through 0.22 µm Sterivex filters (Millipore) on the seafloor ...
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bioRxiv - Bioengineering 2021Quote: ... 48.42 mg of N-(3-Dimethylaminopropyl)-N’-ehtylcarbodiimide hydrochloride (EDC-HCl) (Sigma-Aldrich) were added to the solution along with 27.40 mg of N-hydroxy-sulfosuccinimide (sulfo-NHS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed 3×5 min with 0.1% Tween® 20 (Sigma-Aldrich) in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... and 3 ml of fresh medium containing 0.75% carboxy-methyl-cellulose (Sigma-Aldrich) was added ...
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bioRxiv - Bioengineering 2021Quote: ... Cultures were maintained in the presence of neurotrophin-3 (50ng/ml, Sigma-Aldrich) and brain derived neurotrophic factor (50 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 500 µL of 2% 3-aminopropyltriethoxysilane (Sigma; 919-30-2) in 95% ethanol for 5 min ...
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bioRxiv - Bioengineering 2019Quote: ... and mixed with 10 mg N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma), 10 mg N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were individually embedded in 3% agarose (Sigma Aldrich, St. Louis, USA), dehydrated and embedded in epoxy resin (44611-4 ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mL of 1% alcian blue in 3% acetic acid (Sigma-Aldrich, B8438), and 10 mL water was prepared ...
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bioRxiv - Developmental Biology 2020Quote: T7-promoter sequence- and 3’ overhang-containing DNA oligos were synthesized (Sigma-Aldrich), annealed to a common tracer oligo (AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC) ...
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bioRxiv - Developmental Biology 2020Quote: ... The completed plasmid was transformed into Rosetta BL21 cells (Millipore Sigma, 70954-3). A starter culture of 5 ml of Rosetta BL21 cells containing the completed plasmid were grown overnight at 37°C in LB with Kanamycin ...
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bioRxiv - Neuroscience 2019Quote: ... neurons were incubated with 3 µg/ml Fluorescein isothiocyanate (FITC)-conjugated IB4 (Sigma) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... then visualized using 400 μL 8 mg/mL 3-amino-9-ethylcarbazole (Sigma) in 10 mL 50 mM sodium acetate (Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilised for 3 minutes at room temperature with 0.1 % Triton-X-100 (Sigma) in DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... 3% (w/w) H2O2 stock (Sigma-Aldrich, Saint Louis, MO, Catalog #323381-25ML) was stored in 100 μL aliquots at −20°C.
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bioRxiv - Cancer Biology 2021Quote: ... concentrated with an Amicon Ultra Centrifugal Filter Units (3 kDa cutoff, Millipore UFC900324) and buffer exchanged with 50 mM Ammonium bicarbonate (Sigma A6141) ...
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bioRxiv - Cell Biology 2020Quote: ... This basal medium was supplemented with 2i-LIF (3 µM CHIR99021, Sigma-Aldrich, cat ...
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bioRxiv - Biochemistry 2021Quote: ... Flow cells were regenerated using 3 M GuHCl (Sigma Aldrich, Saint Louis USA). Sensorgrams were analysed ...
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bioRxiv - Biochemistry 2021Quote: ... Serum free medium was concentrated using 3 kDa cut-off filters (Sigma-Aldrich). Next ...
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bioRxiv - Biochemistry 2021Quote: ... pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Later the harvested brains were incubated in 3 mL of 99.5% formamide (Sigma) for 48 hours at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... media was spin-concentrated using a 3 kDa MWCO centrifugal unit (Millipore-Sigma) and the concentrated conditioned media was analyzed by Luminex.
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bioRxiv - Cell Biology 2021Quote: ... media was spin-concentrated using a 3 kDa MWCO centrifugal unit (Millipore-Sigma) and the concentrated conditioned media was analyzed by Luminex.
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bioRxiv - Immunology 2021Quote: ... mtDNA depletion was induced by 100 μM 2′,3′ dideoxycytidine (ddC, Sigma-Aldrich) in medium supplemented with uridine and sodium pyruvate ...