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Citations for Millipore Sigma :
6151 - 6200 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibody was detected using 1:20,000 dilution of HRP-conjugated anti-human IgG (Sigma) and plates developed using TMB substrate (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Human astrocytes were cultured on 0.1 mg/ml poly-D-Lysine (PDL; P6407, Sigma-Aldrich)-coated cover slips in 24 wells for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Both human and mouse retinal explants were flat mounted on polytetrafluoroethylene organotypic filters (Millipore-Sigma) with the photoreceptor side down ...
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bioRxiv - Neuroscience 2023Quote: SH-Sy5y human and N2a (Neuro-2a) murine cell lines were obtained from Sigma (US) and ATCC (US) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA encoding human PF4 ORF was cloned into the pET-15b vector (Novagen, Madison, WI) and transformed into Origami B (DE3 ...
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bioRxiv - Neuroscience 2023Quote: Human Embryonic Kidney 293T (HEK293T) cells were cultured in high-glucose DMEM containing glutamine (Sigma) supplemented with 10% FBS and 2% Penicillin-Streptomycin ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063, Sigma-Aldrich) ∼ 46 h later ...
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bioRxiv - Microbiology 2023Quote: Adhesion of Leptospira strains (Manilae WT, Δmpl36, Δmpl36+, and Patoc) to human PLG (Sigma-Aldrich) was assessed by ELISA as previously described [23] ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with polyclonal rabbit antibodies recognizing human PLG (Sigma- Aldrich, 1:500), followed by peroxidase-conjugated secondary antibodies (1:10,000) ...
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bioRxiv - Cell Biology 2023Quote: ... was from Thermo Fisher Scientific (Waltham, MA) and human tissue plasminogen activator (tPA) from Sigma Aldrich (St. Louis, MO). Rabbit monoclonal anti-PAI-1 [EPR17796] (ab187263 ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were maintained in fresh human erythrocytes at 2% hematocrit in complete RPMI-1640 (Sigma) medium in addition to 0.5% AlbuMaxII (Gibco by Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Bound IgA or IgG was detected using alkaline phosphatase-conjugated goat anti-human IgA (Sigma) or goat anti-human IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Biophysics 2023Quote: ... The anti-human Fc capturing mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 was purchased from EMD Millipore (Billerica ...
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bioRxiv - Cancer Biology 2023Quote: ... DMEM medium supplemented with 10% human serum (male AB, H6914-100ml Batch SLBT2873, Sigma-Aldrich), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... human CD3+ T cells were stimulated with 10ng/ml PMA and 1μM Ionomycin (Sigma-Aldrich) or with 1 μg/mL pre-coated α-CD3 and 1 μg/mL soluble α-CD28 ...
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bioRxiv - Cell Biology 2023Quote: ... Human colorectal adenocarcinoma cells (Caco-2, ATCC) were maintained in medium consisting of DMEM (Sigma), 100 U/ml penicillin ...
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bioRxiv - Immunology 2023Quote: ... Human acute monocytic leukemia cell line (THP-1) was cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was incubated in Sudan Black (0.3% Sudan Black B (Sigma, Cat# 199664) in 70% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µL of anti-human IgG (Fc specific)-peroxidase antibody produced in goat (Sigma, A0170) was added to each sample in a 1:40,000 dilution of PBS and incubated for 1 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Pre-clinical Repository).
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bioRxiv - Immunology 2023Quote: ... 20 mg of purified human IgG in PBS was immobilised to CNBr-activated Sepharose (Sigma) (10 mg IgG/ml packed Sepharose) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Biochemistry 2023Quote: ... sub-cultured until mid-exponential phase then opsonized in human serum (Sigma-Aldrich, no. H4522) for 30 min ...
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bioRxiv - Immunology 2023Quote: Human macrophages were harvested from culture using PBS+5mM Ethylenediaminetetraacetic acid (EDTA; Sigma Aldrich #E7889) and cell lifters (Fisher Scientific #08-100-240) ...
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bioRxiv - Immunology 2023Quote: ... diluted with a 10x volume of RPMI with 10% heat-inactivated human AB serum (Sigma), and centrifuged at 300 x g for 7 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Monocytes were selected by adhering PBMCs in RPMI with 5% pooled AB human serum (Sigma), 1 mM HEPES (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... followed by incubation with CF-647 conjugated anti-human IgG (Sigma Aldrich, cat. no. SAB4600181) diluted 1:500 in cell staining buffer for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by the adding 50 µg/ml (1:100) of human plasma fibronectin (Sigma-Aldrich) for overnight incubation at 4°C.
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines from culture supernatants were analyzed using human cytokine MILLIPLEX Luminex (EMD Millipore). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... 10 µg/L human epidermal growth factor (hEGF; E9644, Sigma-Aldrich, St. Louis, MO, USA), 1% ITS-X supplement (094-06761 ...
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bioRxiv - Bioengineering 2024Quote: ... AS Diagnostik) and polyclonal rabbit anti-human FHL2 (2 µg/mL, HPA006028, Sigma/Prestige Antibodies) was performed at 4 °C overnight in PBS/10% AB serum ...
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bioRxiv - Neuroscience 2024Quote: ... sections were double-immunolabeled for human nuclei and either Htt (1:200; clone EM48, Millipore) or IBA-1 (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... which were coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO; C7521). Ex Plus medium was added to both the apical and basolateral compartments and changed daily until the cells reached confluency on the inserts ...
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bioRxiv - Microbiology 2024Quote: ... TSB-grown bacteria were centrifuged and resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 °C with shaking (180 rpm) ...
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bioRxiv - Biochemistry 2024Quote: ... Human CXCL12 [50 ng/mL] and EGF [50 ng/mL] (Sigma Aldrich SRP3276 and SRP6253) were prepared in serum-free DMEM and used as chemoattractant in the lower chamber ...
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bioRxiv - Neuroscience 2024Quote: ... Downregulation of PAK6 expression was performed utilizing an shRNA against human PAK6 (sh-1944, Sigma). Western blot in SH-SY5Y cell extracts was performed with anti-PAK6 (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... 2-3-week-old plants were treated with 150µM DCMU (D2425-100G, Sigma), kept for 1 hour in the dark and then exposed to high light (HL ...
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bioRxiv - Biophysics 2021Quote: ... Ultrapure water from a Direct-Q 3 UV water purification system (Millipore, USA) was used for all experiments.
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bioRxiv - Cell Biology 2020Quote: ... and 3 μM CHIR99021 on 0.1% gelatin (Catalog No. ES-006-B, Millipore)-coated plates (for the 2i condition) ...
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bioRxiv - Cell Biology 2019Quote: ... Fractions were analyzed by immunoblotting using an antibody to PC1/3 (Sigma, #SAB1100415). Band intensity was quantified using FIJI and plotted against the sucrose concentration of the fraction.
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bioRxiv - Cell Biology 2019Quote: ... all coverslips were incubated with a 5% 3-aminopropyltrimethoxysilane solution (281778, Sigma-Aldrich) in water for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: Cells were plated at 3*105 cells/well in fibronectin-treated (EMD Millipore) 96-well glass-bottom plates (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ml of culture was supplemented with 3 mg/ml propidium iodide (Sigma; stock 1 mg/ml in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000037282 (shRNA Trim39#2) and TRCN0000438509 (shRNA Trim39#3) were from Sigma-Aldrich. Lentiviral particles were produced as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... bafilomycin A1 and 3-methyladenine were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich), an equivalent volume of DMSO was added to control samples.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The plates were washed 3 times with 0.05% Tween-20 (P2287, Sigma-Aldrich) in PBS ...