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Citations for Millipore Sigma :
6251 - 6300 of 7003 citations for CYBB Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated with PBS + 1% milk solution containing a 1:10.000 anti-rabbit horseradish peroxidase conjugated secondary antibody (A0545, Sigma-Aldrich). The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated for 5 min with Pierce ECL Plus substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Immunology 2022Quote: ... The membrane was then washed three times and incubated for one hour with HRP-conjugated goat anti-rabbit IgG (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Pathology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
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bioRxiv - Plant Biology 2022Quote: ... followed by incubation in a 1:10000 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... we confirmed targeting of the optic-fiber-tetrode bundle to LC by colocalization of the electrolytic lesion with immunostaining against tyrosine hydroxylase (rabbit anti-TH, ab152, Millipore, followed by donkey anti-rabbit Alexa 488 ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the samples were incubated with primary antibodies (anti-β-tubulin III produced in rabbit (Sigma, Ireland, T2200, 1:400) and anti-GFAP produced in mouse (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were incubated at 4°C for 16 hours with rabbit anti-Myc (1:500, C3956; Sigma-Aldrich) primary antibody in PBS/0.5% normal donkey serum/0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by probing with respective goat anti-mouse or rabbit secondary antibodies conjugated with alkaline phosphatase (dilution 1: 5,000; Sigma-Aldrich) or conjugated with HRP (dilution 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... for 120 minutes followed by overnight incubation at 4°C with a 1/200 dilution of the poly-clonal rabbit sera and antibodies for histone H3 (final concentration 2.5 μg/mL, Sigma SAB4200651) in blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-human βIII-tubulin (1:500; Covance, cat. MRB-435P) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Primary antibodies were tagged using either goat anti-mouse AF647 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for all stainings were as follows: rabbit anti-TGFA (HPA042297, Sigma-Aldrich, dilution 1:50 and 1:100), rabbit polyclonal anti-pEGFR clone Tyr845 (07-820 ...
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bioRxiv - Cell Biology 2022Quote: ... Dylight 550-labeled rabbit anti-BUB-1 (custom made, 74) and FITC-labeled mouse anti-α-tubulin (SIGMA F2168, DM1α) were used at a concentration of 1 μg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... with Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... protein isolation and immunoprecipitation was as previously described (Mohammed et al., 2016) using 1×107 OL-differentiated NSCs incubated with rabbit anti-OLIG2 (AB9610, Millipore) and using rabbit pre-immune IgG as control ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase, A3682, Sigma) were visualized using Amersham ECL or ECL Prime 225 reagent (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... Then the membrane was incubated with another primary serum and revealed using an anti-rabbit immunoglobulin G coupled to alkaline phosphatase (Sigma). Data were acquired from three or four independent experiments and representative immunoblots are shown ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used: Rabbit anti-Orai1 (1:50, from Dr. Stefan Feske) and mouse anti-ORAI1 (1:50, SAB3500126, SIGMA), rabbit anti-STIM1 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... before microtubule-associated protein light chain 3 (LC3)-I and LC3-II levels in cell lysates was analysed by immunoblotting (rabbit polyclonal LC3 antibody, Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes previously incubated with anti-RplA were incubated with the secondary antibody anti-rabbit IgG-HRP (#A8275, 1:10000, Sigma) for 1 h at room temperature and then washed three times with T-PBS ...
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bioRxiv - Neuroscience 2024Quote: ... followed the same staining and mounting protocol described above but were incubated ON with a dilution of 1/2,000 anti-HA rabbit antibody (Sigma; H6908), and a 1/1000 dilution of AlexaFluor-488 donkey anti-chicken (Jackson InmunoResearch ...
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bioRxiv - Cancer Biology 2024Quote: ... was used as the primary antibody and a goat polyclonal anti-rabbit IgG conjugated to horseradish peroxidase (Millipore, 12-348) for the secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated overnight in primary antibody Anti-Tyrosine Hydroxylase Antibody from rabbit (1:500, cat# AB152, Sigma Aldrich) diluted in blocking solution at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were incubated overnight at 4°C in the presence of primary antibodies in PBS-T: rabbit PYK2 antibody (1/500, Sigma #07M4755 ...
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bioRxiv - Neuroscience 2024Quote: ... Whole tissue cultures were incubated with rabbit anti-Iba1 (1:1000; Fujifilm Wako, #019–19741) or mouse anti-GFAP (1:1000; Sigma) in PBS containing 10% (v/v ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and ADP production was quantitatively coupled to the stoichiometric consumption of NADH via the activity of excess pyruvate kinase (PK, type-III from rabbit muscle, Sigma) and lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2024Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 5% milk-PBST). Anti-ATS and anti-NapL were detected as previously described (Nacer et al ...
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bioRxiv - Microbiology 2024Quote: ... and MreB or Spo0J were detected using respective in-house polyclonal antibodies and HRP-conjugated anti-rabbit IgG antibody (Sigma). Samples were developed using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Bioengineering 2024Quote: ... MyoD (primary Anti-MyoD Antibody, #ZRB1452, Sigma-Aldrich, and an anti-rabbit secondary antibody coupled with Rhodamine #SAB3700846, Sigma-Aldrich), DAPI (4′-6-diamidino-2-phenylindol ...
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bioRxiv - Cell Biology 2024Quote: ... The slides were probed with anti-PfMFS_DTHydrophillic mouse serum (1:100) and anti-PfMSP1 rabbit serum (1:300; Sigma-Aldrich) for 1 hour at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate production was enzymatically quantified as NADH fluorescence (360 nm/460 nm) by the reverse reaction of L-lactate dehydrogenase (Rabbit muscle, L25005KU, Sigma) in a reaction containing 20 µL cell media ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoblots of BN-PAGE analysis were decorated with HRP-coupled goat anti-mouse or HRP-coupled goat anti-rabbit (both Sigma) as secondary antibodies (dilution ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... then transferred to 10 µL drops of equilibrated and pre-warmed rabbit culture medium covered with mineral oil (Sigma, M8410). Embryos were maintained in an incubator (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Expression of WT and mutant N was assessed by Western blot using a rabbit anti-N antiserum 39 and a mouse monoclonal anti-tubulin antibody (Sigma), revealed by incubation with anti-rabbit and anti-mouse antibodies coupled to HRP (SeraCare).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...