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Citations for Millipore Sigma :
6401 - 6450 of 7003 citations for CYBB Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and slices were incubated overnight at 4 °C in a PBST-based antibody carrier solution containing 3% normal goat serum and rabbit anti-TPH2 primary antibodies (Millipore, ABN60) diluted 1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... This was followed by a rinse with phosphate buffered saline and application of the diluted primary antibodies: whole molecule Anti-Rabbit IgG (1:200, goat, FITC antibody, Sigma-Aldrich) then secondary antibodies (Whole molecule ...
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bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... It was followed with further washes and incubation at room temperature with TRITC conjugated anti-rabbit IgG (1:400) (Sigma, USA) for 1h ...
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bioRxiv - Zoology 2019Quote: ... The blocked specimens were incubated in mixture of rabbit anti-5-HT (S5545, Sigma-Aldrich, St. Louis, USA, diluted 1:1000) and rabbit anti-FMRFamide antibodies (AB15348 ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with HRP-coupled secondary antibodies diluted 1:10,000 in BS (anti-mouse IgG Jackson 515-035-062, anti-rabbit IgG Sigma A-9169), washed twice in BS and twice in TBS ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 rabbit anti-β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were then incubated overnight at 4°C with rabbit polyclonal to elafin in 2% BSA at 1:100 dilution (Sigma, HPA017737). The signal was detected using the rabbit horseradish peroxidase EnVision kit (DAKOCytomation ...
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bioRxiv - Neuroscience 2019Quote: ... In embryonic tissue antibody labeling was used to label pH3 in neural progenitors (1:500; rabbit; Millipore, CAT#:06-570, RRID:AB_310177). Adult tissue was either co-stained in 1:100,000 DAPI in PBS to facilitate the delineation of brain structures or was labeled for MOR (1:3000 ...
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bioRxiv - Microbiology 2019Quote: ... For antigen-antibody detection membranes were incubated for 1h at room temperature with a 1:2500 dilution of anti-rabbit IgG (whole molecule)— alkaline phosphatase antibody produced in goat (Sigma A0545) in PBS pH7.4 ...
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bioRxiv - Physiology 2019Quote: ... was used at a dilution of 1/500 and rabbit polyclonal antibody against calnexin (C4731; Sigma-Aldrich, Saint-Louis Missouri, USA) was used at 1/1000.
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bioRxiv - Biochemistry 2019Quote: ... rabbit monoclonal DYKDDDDK tag (Flag tag, 1:1000, CST, #14793) and/or mouse monoclonal α-tubulin (1:10,000, Millipore, 05-829). Following three washes with TBS-T ...
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bioRxiv - Cell Biology 2019Quote: ... The RBM proteins were examined with the primary antibodies and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the infected cells were fixed with cold acetone and tested using CPV-2-specific canine antibodies and rabbit anti-dog IgG conjugated with fluorescein isothiocyanate (Sigma Aldrich). Viral titre ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Immunology 2019Quote: ... The microarray was then rinsed for 3 times with washing buffer and incubated with 200 μL of Horseradish peroxidase (HRP) conjugated Rabbit anti-Goat IgG (Sigma-Aldrich) diluted 1:20000 in Peroxidase Conjugate Stabilizer/Diluent (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Protein samples of PSD95 and Kal7 or PSD and Kal8 were incubated at 4oC overnight with rabbit anti-Flag Antibody (#F7425 Sigma; RRID:AB_439687) in a final volume of 120μl 20mM TES ...
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bioRxiv - Neuroscience 2020Quote: ... The specimens were incubated at room temperature for 1 h in blocking buffer (PBSTX with 10% normal sheep serum and 2% bovine serum albumin) and subsequently with the primary antibody (rabbit-anti-GABA, Sigma-Aldrich A2052 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated with secondary anti-Rabbit IgG-Gold coupled antibody (1/10 in BHN1) (Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat (Sigma, G7652), washed twice in BHN2 and HN (50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times in PB and incubated for 24 hours at 4°C under continuous stirring with rabbit primary polyclonal antibody against Cy3-conjugated NeuN (Millipore, ABN78C3) diluted 1:1000 in PB ...
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bioRxiv - Cell Biology 2021Quote: ... proximity ligation assays were performed on isolated HSCs with the Duolink in Situ Red Starter Kit Mouse/Rabbit (DUO92101, Millipore Sigma), adapting the vendor’s protocol for HSCs ...
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bioRxiv - Neuroscience 2020Quote: ... as well as secondary anti-mouse and anti-rabbit (Thermo Fischer Scientific) Membranes were incubated with Immobilon® ECL Ultra Western HRP Substrate (Millipore) for 3 minutes prior to image acquisition ...
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bioRxiv - Cancer Biology 2019Quote: ... they were incubated in the washing buffer under agitation (50 rpm) at 4°C for 1 hr with 1 μg/mL of rabbit anti-Flag- (SIGMA, F7425) or mouse anti-SUMO-1 (21C7 from the Developmental Studies Hybridoma Bank ...
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bioRxiv - Neuroscience 2021Quote: ... After three washes in PBS sections were incubated with secondary antibodies (anti-Rabbit IgG CF™ 568, 1:1000, Sigma Aldrich, SAB4600084 ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslip were washed with PBS with 0.25% triton X-100 and followed by incubation with secondary antibodies Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:1000) together with Hoechst 33342 1 µM (14533; Sigma-Aldrich) for nuclei staining during 1h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were incubated at 37 °C overnight in a 1.5% BSA solution with anti-β-III-tubulin (anti-rabbit, 1:500, Sigma-Aldrich) and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Neuroscience 2021Quote: ... MAP2 and/or PSD-95 were immunostained using primary antibodies diluted in PBS-BSA 1% for 30 min (SP: rabbit polyclonal, Synaptic System, 163 002, 1:600; VGLUT1: guinea pig polyclonal, Merck Millipore, AB5905 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an overnight exposure at 4 °C in the same buffer to the following primary antibodies: (a) rabbit anti-mCherry polyclonal antibody (1:5000, Millipore #AB356482); (b ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... we used rabbit polyclonal anti-AFG3L1 (Koppen et al, 2007) (Figure 1A, B and Figure S1C) and rabbit polyclonal anti-FLAG (#F7425 from Sigma-Aldrich) (Figure 3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections incubated at room temperature overnight in primary antibody medium or control medium (1:100 primary anti β-catenin antibody from rabbit Sigma C2206 + 1:100 primary anti β-actin antibody from mouse Sigma A5441 + PBS pre-incubation medium ...
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bioRxiv - Cancer Biology 2019Quote: ... The samples were incubated overnight at 4 °C with the indicated primary antibody prepared in blocking/permeabilization solution (with 0.1% Triton X-100): rabbit polyclonal antibody against GLUT-1 (1:250; Millipore, 07-1401), rabbit monoclonal antibody against HK-1 (1:200 ...
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bioRxiv - Physiology 2021Quote: ... Protein detection was performed by the use of the HRP-conjugated secondary antibodies: anti-rabbit (CST #7074) or anti-mouse (CST #7076) and Immobilon Forte Western HRP substrate (Millipore Sigma). Protein band intensity on exposed film was measured with Adobe Photoshop software.
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bioRxiv - Biochemistry 2021Quote: ... the membrane was washed with TBS-T and incubated for 90 min with anti-rabbit IgG antibody conjugated to alkaline phosphatase (Sigma Alrich), diluted 1:5.000 in TBS-T solution containing 0.75 % BSA ...
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bioRxiv - Biochemistry 2020Quote: ... Chemiluminescence of the appropriate secondary rabbit or mouse HRP-conjugated antibodies after incubation with ECL HRP substrate (Immobilon™ Western HRP Substrate, Millipore) was detected using a Chemi Doc™ Quantity One® system (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
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bioRxiv - Microbiology 2020Quote: ... were used with secondary anti-mouse IgG or anti-rabbit IgG conjugated with horseradish peroxidase antibodies (A9917 and AP132P, Sigma Aldrich). SuperSignal West Pico Chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight with primary antibody against tyrosine hydroxylase diluted in 2% NDS in PBS/TX (1:1000 - rabbit anti-TH, Millipore AB152). The sections were rinsed with PBS (3×10min ...
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bioRxiv - Neuroscience 2020Quote: Cell type specific imaging using fluorescent markers: dopaminergic neurons were specifically labelled using the anti-Tyrosine Hydroxylase (TH) antibody (1:250, rabbit polyclonal, AB152, Sigma Aldrich). This was done on 50 μm thick coronal sections in the midbrain area specifically for the neurons in the ventral tegmental area (VTA ...
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bioRxiv - Neuroscience 2020Quote: ... Position in the retina was determined by staining with a primary antibody sensitive to S-opsin (rabbit-anti-s-opsin, 1:500, Millipore ABN1660) (Sondereker et al. ...
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bioRxiv - Microbiology 2021Quote: ... then washed and probed with a 1:2,000 dilution of an α-rabbit alkaline phosphatase conjugated antibody (Sigma Aldrich, cat # A3687) for one hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The cells were lysed in 5X Laemmli buffer and the lysates were subjected to SDS-PAGE followed by immunoblotting as previously described using an 1/10000 diluted anti-TEM rabbit serum and an 1/10000 diluted anti-actin antibodies (Sigma-Aldricht).
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies (rabbit anti-UCP1, 1:750, Abcam ab10983, mouse anti-tyrosine hydroxylase, 1:1,500, Sigma-Aldrich T1299) in blocking buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Mouse phospho-MLKL (Ser345) rabbit monoclonal (D6E3G) (Cell Signalling Technology) MLKL rat monoclonal 3H1 (available from Millipore MABC604; generated in-house) ß-actin mouse monoclonal AC-15 (Sigma A-1978)