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Citations for Millipore Sigma :
6151 - 6200 of 7003 citations for CYBB Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... followed by incubation in a 1:200 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot of phosphorylated MAPKs was performed using anti-phospho p44/42 MAPK antibody as the primary antibody and peroxidase-conjugated goat anti-rabbit IgG (Sigma) as the secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Microbiology 2021Quote: ... The deposited C5b9 complex in the reaction mixture was detected using rabbit anti-C5b-9 pAb at 1:1000 dilution (204903; Sigma) and HRP conjugated anti-rabbit pAb (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... in 5% BSA-TTBS overnight at 4°C and then HRP-conjugated goat anti-rabbit IgG (Jackson) or goat anti-mouse IgG(Sigma) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Flag (M2) and anti-MAP2 (HM-2) antibodies as well as polyclonal rabbit anti-Flag antibodies (F3165, F7425) were from Sigma. Anti-Xpress antibodies were from Invitrogen (46-0528).
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bioRxiv - Neuroscience 2022Quote: ... 0.3% Triton X-100 in PBS for 1 hour and then incubated with rabbit anti-TH (1:1000; Millipore: AB1542) and sheep anti-fibrinogen (1:300 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell line showing reduced intensity or no bands in western blot using rabbit anti-horseradish peroxidase antibody (P7899, Sigma-Aldrich) which can recognize core α1,3-fucose and β1,2-xylose were propagated and used for further analysis (supplementary figure 1 ...
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bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...
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bioRxiv - Immunology 2022Quote: ... SAMD9 knock-down was confirmed by Western Blot using rabbit anti-SAMD9 antibody (Cat# HPA021319, Millipore-Sigma, St. Louis, MO) and goat-anti-rabbit HRP secondary antibody (Cat# 111-035-144 ...
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bioRxiv - Immunology 2022Quote: ... SAMD9 knock-down was confirmed by Western Blot using rabbit anti-SAMD9 antibody (Cat# HPA021319, Millipore-Sigma, St. Louis, MO) and goat-anti-rabbit HRP secondary antibody (Cat# 111-035-144 ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed as before and 100μl/well rabbit anti-mouse IgG1 or IgG2a conjugated to HRP (Sigma-Aldrich, Germany) diluted to 1 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: sections were incubated in a solution containing a rabbit anti-orexin A antibody (1:1000; AB3704, Millipore, Temecula, CA, USA) for two days followed by incubation in a solution containing Alexa-Fluor Plus 594 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed three times with 1 X TBST and incubated for 1 h with 1:10,000 diluted secondary antibody (Anti-Rabbit IgG peroxidase, Sigma-Aldrich) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies were goat anti-rabbit and anti-mouse Alexa 488 and Alexa 555 conjugated fluorescence at 1:500 (Millipore). The spinal cord sections were directly coverslipped with Dapi mounting media (Vector ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Cell Biology 2022Quote: ... The next day cells were washed 2x in PBS and then incubated with pre-mixed PLA probe anti-goat minus and PLA probe anti-rabbit plus (Sigma) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 2 h with horseradish peroxidase (HRP)-conjugated secondary antibody (12-341 Rabbit anti-chicken RAC-HRP, Sigma-Aldrich) diluted 1:15000 in BSA-TBST ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... In this case the blot was incubated with the IcdH primary antibody at room temperature for 2 hours and with the secondary antibody (anti-rabbit HRP, Sigma) was done at room temperature for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were stained with rabbit anti-Actin or rabbit anti-TnI (1:1000; a gift from A. Ferrus) or mouse anti-Tubulin (1:1000, Sigma) and washed with TBS-Triton X (0.1%) ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for 10 min before sections were incubated at room temperate overnight in a humidified chamber with primary polyclonal rabbit anti-TH antibody at 1:500 (ab152, Millipore). Primary antibody was washed off sections for 5 min in TBS before sections were incubated with biotinylated goat anti-rabbit secondary antibody at 1:500 at room temperature for 1 hour (BA-1000 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoreactivity was detected using the avidin-biotin detection method (biotinylated donkey-anti rabbit IgG (Sigma-Aldrich, Søborg, Denmark, #SAB3700966); avidin-biotin-peroxidase complex (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... then the Duolink In Situ PLA Probe Anti-Rabbit PLUS and Duolink In Situ PLA Probe Anti-Mouse MINUS PLA probes (Sigma) diluted in Duolink Antibody Diluent were applied ...
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bioRxiv - Cancer Biology 2021Quote: ... The PLA was performed using reagents supplied in the Duolink in situ Red PLA Mouse/Rabbit kit (Sigma-Aldrich, DUO92101) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times and incubated with a 1:10,000 dilution of horseradish peroxidase-conjugated anti-rabbit antibodies ((Sigma-Aldrich) for 1-2 h ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized with 0.3% Triton X-100 in PBS (PBST) for 10 minutes and incubated with rabbit primary antibody against TH (1:1000, AB152; Millipore/Sigma) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: One series of sections was washed with KPBS to remove the cryoprotectant solution and then incubated with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with OXT and AVP primary antibodies (mouse anti-oxytoxin, Gainer Lab, NIH, PS3845,60 and rabbit anti-vasopressin, Millipore PC234L) diluted in 0.1 % saponin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... free-floating sections were washed with KPBS to remove the cryoprotectant solution and then incubated directly with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Biophysics 2021Quote: ... Samples were washed 3×5 min using 1% BSA in PBS followed by 60 min staining using a 1:2000 dilution of the anti-rabbit Atto647N antibody (Sigma). Samples were washed 3×5 min with PBS and stored in light protected container in +4° C until imaged ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2, 1:1000, rabbit, Millipore). Sections were washed 3 times for 10 mins each in 1x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in the dark in Alexa 555-conjugated or Alexa 488-conjugated goat anti-rabbit-IgG (1:300; Millipore Sigma) for 2 h following primary antibody incubation ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... we added polyclonal antibodies against the extracellular domain of TrkB (1:50 rabbit anti-TrkB, Millipore, cat no. AB9872, RRID:AB_2236301) together with 20 ng/mL BDNF for 2.5 hours ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
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bioRxiv - Biochemistry 2019Quote: ... the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific ...
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bioRxiv - Physiology 2019Quote: ... fiber type IIb) (hybridoma supernates, Developmental Studies Hybridoma Bank) supplemented with rabbit polyclonal anti-laminin antibody (2 μg/ml, Sigma) overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: ... Western-blot analysis was carried out using anti-LC3-II (1:1000, rabbit polyclonal, Sigma-Aldrich, Saint-Quentin Fallavier, France), anti-ß-tubulin (1:1000 ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin, Sigma S5545 or rabbit anti FMRF-amide ...