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Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: 5′end biotin-labeled TP53 3′UTR RNA fragments with different PAS and other mutations and miRNA of interest labeled with biotin at the 3′end were commercially synthesized (Sigma-Aldrich). H1299 cells were seeded one day before transfection in 10 cm tissue culture dish at 50% confluent ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Molecular Biology 2019Quote: ... 3.6 x 107 cells in growth medium were labeled at a final concentration of 500 µM 4-thio-uracil (4sU, Sigma-Aldrich), and incubated at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... 5 × 106 activated B cells were washed with PBS and re-cultured in glucose-free medium RPMI medium containing 10% dialyzed FBS and uniformly labeled [13C]-glucose (2 g/L; Sigma-Aldrich) for 24 and 48 h ...
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bioRxiv - Systems Biology 2019Quote: ... and combinations of the following lysine and arginine isotopologues: for “light” (“L”)–labeled cells L-arginine (84 mg/L) and L-lysine (146 mg/L)(Sigma-Aldrich); for “medium” (“M”)-labeled cells ...
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bioRxiv - Biochemistry 2021Quote: ... intact histones were mixed with 5 μL of deuterium-labeled propionic anhydride (propionic anhydride-d10) rapidly followed by 14 μL of ammonium hydroxide (Sigma-Aldrich) to adjust to pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... pBF2*1501 epitope-specific tetramers were prepared by mixing the biotinylated pBF2*1501 complexes and phycoerythrin (PE)-labeled streptavidin (Sigma Aldrich) at a molar ratio of 4:1 ...
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bioRxiv - Cell Biology 2021Quote: ... The covalently fluorescently labelled nucleosome arrays used for microinjection were labeled at an 8% fluorophore density (8 in 100 histone2B proteins labeled with a fluorophore) and dialyzed against TE (10 mM Tris-HCl (Sigma-Aldrich), pH 7.4 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... click chemistry reactions were performed on pre-permeabilized samples to conjugate biotin to the EdU-labeled DNA by using Biotin Picolyl azide (Sigma Aldrich). Sonication was performed with a Bioruptor Pico sonicator (Diagenode ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Zoology 2023Quote: ... tissues were hybridized with digoxigenin labeled antisense DNA and subsequently exposed to anti-digoxigenin Fab’ fragments conjugated to alkaline phosphatase (Sigma-Aldrich) and peptide primary antisera ...
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bioRxiv - Immunology 2024Quote: ... the trypsin was used as is and the pepsin one was labeled at the C-terminal end using Arginine methyl ester dihydrochloride (Sigma-Aldrich) using the method used in (36).
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bioRxiv - Developmental Biology 2023Quote: ... Full lengthrfx6.L and pdx1.L anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10× DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: Replicating cells and nascent mtDNA were labeled by administering a dose of 100 mg/kg body weight BrdU (Sigma-Aldrich, #B5002) via intraperitoneal injection to P28-29 mice ...
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bioRxiv - Cell Biology 2023Quote: ... The washed nuclei pellets were resuspended in sorting buffer (2% BSA/RNase-free PBS) and labeled with anti-PCM1 (1:500, HPA023374, Sigma-Aldrich) on ice for 45 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and stained with mitochondrial networks labeled with a primary antibody against TOMM20 (Sigma-Aldrich, HPA011562) used at 1:1000 ...
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bioRxiv - Biochemistry 2022Quote: EMSAs for the SAP-domain were performed with DNA such that the single strand or one of the two strands in dsDNA was 5’-labeled with fluorescein (6-FAM, Sigma-Aldrich) (Table 1) ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (Sigma, cat. # F4680).
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bioRxiv - Cancer Biology 2023Quote: All cell lines subjected to this analysis were grown asynchronously and labeled with 30 μM of the thymidine analog chlorodeoxyuridine (CldU; Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded on a 18×24 mm coverslip and the next day they were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Biophysics 2023Quote: ... the cell was infused with 0.5 nM BfiI and 0.2 nM of Cy3B-labeled dsDNA oligonucleotide in imaging buffer (IB, the YB supplemented with 15 units/mL of glucose oxidase (G6125, Sigma-Aldrich), 1% of glucose (G0047 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2024Quote: ... TMT-labeled samples for a given mass spectrometry run were then pooled and acidified with 5% formic acid (Sigma, vol/vol). Samples were concentrated using a SpeedVac and resuspended in buffer A (95% water ...
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bioRxiv - Cancer Biology 2023Quote: CRISPR-Cas13d or Tet-On overexpressing cells were sequentially pulse-labeled with 50 µM 5-chloro-2′-deoxyuridine (CldU, Sigma-Aldrich) and 250 µM 5-iodo-2′-deoxyuridine (IdU ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Dextran 70 kDa was used as a negative control and was also coupled to a rhodamine (Rhodamine B-isothiocyanate-labeled Dextran; R9379, Sigma- Aldrich).
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bioRxiv - Cell Biology 2023Quote: Tubulin was purified from porcine brain as described before (Castoldi and Popov, 2003) and labeled with biotin-N-hydroxysuccinimide (NHS; Sigma-Aldrich) or ATTO-647N-NHS (ATTO-TEC ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 5 units/μl of DNA polymerase in diluted Polymerase Buffer with Red Fluorescent-labeled Oligonucleotides (1:5, Sigma Aldrich) for 100 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... digoxygenin (DIG)-labeled antisense probes were prepared using the Roche DIG labeling kit (Sigma Cat # 11277073910 and T7 (Sigma Cat # 10881767001) or T3 (Sigma Cat # 11031163001 ...
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bioRxiv - Neuroscience 2024Quote: ... The hippocampus was sliced in a transverse orientation and then labeled with PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich) as primary and goat anti-rabbit IgG (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated at 4°C o/n with the monoclonal anti-flag M2 primary antibody (Sigma Aldrich) in TBS-T buffer containing 5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were stained with monoclonal anti-Ki67 raised in rabbit (1:200, 275R-15, Cell Marque/Sigma-Aldrich) for 60 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed with TBS and blocked with TBS with 5% non-fat milk for 1 hour at room temperature and then incubated with mouse monoclonal antibody for Muc5b (1:500 vol:vol) or lectin UEA-1 L8146 1mg/mL (1:1000 vol:vol) (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The pre-cleaned chromatin was then incubated with 2.5 µL of monoclonal H3K4Me3 primary antibody (Millipore, cat. #04-745), while rotating at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Antibodies and dilutions were as follows: Anti-HNK-1 antibody (Sigma Aldrich, Monoclonal Anti-HNK-1/N-CAM (CD57), # C6680) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4 °C with a monoclonal anti-RBPMS antibody (1:500, Rabbit, ABN1362, Merck Millipore) for the retina ...
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bioRxiv - Microbiology 2021Quote: ... Endogenous HA-FLAG-tagged PPS parasite samples were probed with Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and donkey-anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were incubated with a monoclonal anti-myc antibody (Covance, MMS-150R) or anti-Flag antibody (Sigma, F3165) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies and dilutions were as follows: β-tubulin type III monoclonal (TuJ1, 1:500; Sigma, St. Louis, MO), GFAP polyclonal (1:1,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recognition and super-shifting of FLAG-ZNF410 overexpression constructs was achieved by using the anti-FLAG monoclonal antibody (Sigma). Gels were run at 250 V for 1h 45 min at 4 °C then dried under vacuum ...
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bioRxiv - Microbiology 2020Quote: ... diluted (1:2,000) in 5% skimmed milk in PBS containing 0.3% Tween-20 (PBST) and anti-α-tubulin monoclonal antibody (Sigma) (1:45,000 ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Antibodies used for the western blots were obtained from commercial suppliers: anti-actin monoclonal antibody (Sigma-Aldrich A0480-200μL) and anti-mouse antibody (Agrisera AS111772) ...
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bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibodies (POLR2A, Abcam monoclonal antibody, ab5408; SMN, Santa Cruz polyclonal antibody H-195, sc-15320; ACTB, Sigma, A5441) were used at 1:2,000 to 1:5,000 dilutions for incubation overnight followed by three times washes with RIPA buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were incubated overnight at 4 °C with monoclonal anti-FLAG M2 antibody (1:1000, F1804, Sigma-Aldrich, Germany) and polyclonal rabbit anti-H3K9me3 antibody (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... Flow cells were first incubated with 30 μl of 1:100 dilution of monoclonal anti-β-Tubulin antibody (Sigma) for 10 minutes in a humidified chamber ...
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bioRxiv - Cell Biology 2019Quote: ... the monoclonal anti-myc (clone 4A6) and the polyclonal anti-RhoU antibodies were from Millipore (Saint Quentin Fallavier, France), the polyclonal anti ITSN2 were from Novus Biologicals (Lille ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of the target proteins was verified by western blot using ANTI-FLAG M2 Monoclonal Antibody (Sigma-Aldrich). ChIP assay was performed with ANTI-FLAG M2 Magnetic Beads (Sigma-Aldrich ...