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Citations for Millipore Sigma :
5901 - 5950 of 10000+ citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and anti-rabbit IgG whole molecule-Peroxidase (1:80,000; catalog no. A0545, Sigma-Aldrich). Proteins and epitope-tagged proteins on immunoblots were detected using the Clarity ECL Western Blotting Substrate or SuperSignal West Femto Maximum Sensitivity Substrate according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Secondary antibody (anti-rabbit IgG, whole molecule, alkaline phosphatase, Sigma, St. Louis, MO, USA), 200 μl of a 1:2000 dilution in PBS ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibodies used were: HRP GoataMouse IgG(H+L) (1:2000; #AP308P; Sigma Aldrich) and Alexa Fluor 488 GoataRabbit IgG(H+L ...
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bioRxiv - Immunology 2021Quote: ... or 1:5,000 dilution of HRP conjugated anti rabbit IgG secondary antibody (Sigma-Aldrich) for 1 Hr at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... A standard curve consisting of known amounts of purified IgG (Sigma, St. Louis MO) was run in conjunction with each assay ...
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bioRxiv - Cell Biology 2021Quote: ... the secondary antibody was goat anti-rabbit IgG conjugated with alkaline phosphatase (Sigma, A3687), and the membrane was incubated with BCIP/NBT chromogenic substrate (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Abberior®STAR 635 goat anti-rabbit IgG conjugates (1:1000 dilution; Sigma-Aldrich). DNA was stained with Hoechst 33258 (1:5000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... or as control non– immune IgG antibody of rabbit serum (I5006, Sigma-Aldrich, USA) for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary anti-rabbit IgG coupled to peroxidase (Sigma-Aldrich reference A9169, Saint Louis, USA) was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Albumin/IgG was removed from 60 µL serum according to the manufacturer’s instructions (Millipore). The albumin/IgG-depleted serum was concentrated to 60 µL with 10-kDa cut-off centrifugal filter ...
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bioRxiv - Genetics 2022Quote: ... membranes were incubated with peroxidase conjugated anti-rabbit IgG (Sigma Aldrich, A6154, 1:10,000) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... followed by 1:500 Alexa Fluor 488 goat anti-rat IgG antibody (Sigma-Aldrich) conjugated antibody ...
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bioRxiv - Neuroscience 2022Quote: ... anti-guinea pig IgG (H + L) (1/1,000, CF-647, Sigma-Aldrich, No. SAB4600180), anti-rabbit IgG (H + L ...
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bioRxiv - Physiology 2022Quote: ... and anti-rabbit IgG-HRP secondary antibody (1:1500, Sigma Aldrich, St. Louis, MO) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... while the second received 1 microgram of rabbit IgG (Sigma-Aldrich, St. Louis, MO) as a negative control and rotated overnight at 4°C ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase ...
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bioRxiv - Plant Biology 2024Quote: ... The secondary antibody was rabbit anti-rat IgG conjugated to peroxidase (Sigma-Aldrich A5795), used at a 1:8000 dilution in TBS-T with 2.5% dried milk buffer for one hour ...
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bioRxiv - Immunology 2023Quote: ... All IgGs were immediately buffer exchanged to TBS using 30 kDa MWCO concentrators (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... and Rabbit IgG HRP linked whole Ab (Sigma; #NA934-1ML, 1:5000; lot #: 17065614).
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bioRxiv - Pathology 2023Quote: ... Sections were also incubated with a non-immune rabbit IgG antibody (I8140, Sigma-Aldrich) as negative controls ...
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bioRxiv - Microbiology 2023Quote: ... at a 1:2,000 dilution followed by peroxidase-conjugated anti-rabbit IgG (Sigma-Aldrich) at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Purified human IgG and IgA were used as standards (Sigma, St. Louis, MO, USA). Tear samples were tested by 4-fold serial dilution ...
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bioRxiv - Cell Biology 2023Quote: ... USA.The anti-Rabbit IgG (A4914) HRP-conjugated secondary antibody was purchased from Sigma Aldrich, Rockville ...
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bioRxiv - Synthetic Biology 2024Quote: ... The primary and secondary antibodies were 1:2000 rabbit α-GAPDH (Sigma-Aldrich)/3% BSA/TBSA and 1:20,000 HRP-conjugated goat α-rabbit IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Neuroscience 2024Quote: ... and and donkey anti-chicken IgG conjugated to Alexa-647 (1:500, Millipore, AP194SA6) in PBS or HBS/5% GS for 60 min.
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bioRxiv - Microbiology 2024Quote: ... and antigen-reactive clones were expanded in serum IgG-free RPMI-1640 (Sigma-Aldrich) into roller bottles at 37°C ...
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bioRxiv - Genetics 2021Quote: ... mice were treated with E2 (6.7 ng per mouse) and P4 (P0130, Sigma-Aldrich; 1 mg per mouse) for 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... The primary antibodies were mouse anti-KCa1.1 (1:500; NeuroMab) and mouse anti-α-tubulin (1:10,000; Sigma).
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Cell Biology 2021Quote: ... goat-anti-mouse and goat anti-rabbit Abberior STAR 635P and goat anti-mouse Atto 647N (Sigma-Aldrich), together with Atto 647N Phalloidin (ATTO-TEC ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Zrf1 (Gfap; IgG1; ZIRC 1:20) and mouse anti-acetylated tubulin (AT; IgG2b; Sigma 1:800). Secondary were all raised in goat and included anti-rabbit conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-vinculin (mouse) and anti-LC3 (mouse) antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemiluminescence reagent for Western blot was obtained from BioRad Laboratories (Hercules ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then labeled with the PLA antimouse PLUS probe (DUO92001) and the PLA anti-rabbit MINUS probe (DUO92005) purchased from Sigma-Aldrich. Detection was performed with Duolink in situ detection reagents red kit (DUO92008 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were fluorescently labeled using methyl green (Prieto et al, 2014) and actin filaments with TRITC-conjugated phalloidin (Sigma-Aldrich, P1951). Observation of whole embryos was performed using a Zeiss LSM 880 or a Zeiss LSM 800 laser confocal microscope ...
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bioRxiv - Molecular Biology 2020Quote: HPG labeled proteins in supernatant and pellet fractions (∼1 mg) were click-conjugated with 50 µM azide-PEG3-biotin (Sigma-Aldrich) by a Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were rinsed 6 times in 1x PBS before being incubated with fluorescence-labeled secondary antibody (diluted in blocking buffer at 1:500 dilution ratio with 1/10000 Hoechst 33258, Sigma #94403) for 1 hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL of DIG labeled probe was diluted in 100 μL prehybridization buffer containing 400 ng tRNA (Sigma-Aldrich, St. Louis) and denatured at 70°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... flat 1:30 PDMS stamps were incubated for 1 h with 40 mL of 50 mg/mL Alexa Fluor 647–labeled and 50 mg/mL unlabeled fibronectin (F1141; Sigma-Aldrich), then washed and dried ...
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bioRxiv - Biochemistry 2020Quote: Rhodamine-labeled microtubules were polymerized from bovine tubulin (~10% final rhodamine-labeled tubulin, prepared as previously and stabilized with 10 μM taxol (Sigma T7191) before being pelleted in a TLA120.1 rotor at 90,000 rpm for 10 min at 25°C (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule polymerization was initiated by flowing in 20 mM 1:25 rhodamine-labeled porcine tubulin in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrich), 50 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed in DPBS (5 minutes each wash) and incubated with 2.5 µg/ml an FITC-labeled anti-HA antibody (Sigma, Product # H7411) diluted with 5% BSA in DPBS for 1.5 h at room temperature ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma) overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The FISH hybridization mixture (20 ng of the labeled probe, 10 μg of salmon sperm, 50% of deionized formamide (Sigma-Aldrich)) in 2 x SSC was incubated on slides at 82 °C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde and stained with mitochondrial networks labeled with a primary antibody against TOMM20 (Sigma-Aldrich, HPA011562) used at 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10X DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-sense DIG labeled in- situ probes were generated using linearized plasmid cDNA templates with the10X DIG RNA labeling mix (Sigma 11277073910) according to manufacturer’s instructions.