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Citations for Millipore Sigma :
5801 - 5850 of 10000+ citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Free dye was removed from the labeled proteins by successive concentrations and dilutions using a 3 K MWCO centrifugal concentrator (Amicon Ultra-4, Millipore). The labeling efficiency was computed by measuring the concentration of the protein and Cy3 dye using their absorbances at 280 nm and 550 nm ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were stained with primary antibodies diluted 1:1,000 in TTBS followed by incubation with horse peroxidase-labeled secondary antibody (Millipore Sigma) diluted 1:10,000 in 2% non-fat milk in TTBS ...
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bioRxiv - Bioengineering 2020Quote: ... Ten µl of the C18 SPE purified extracts were DMB-labeled with 60 µl of DMB labeling reagent (Sigma-Aldrich), at 50°C for 2.5 h in the dark under shaking conditions at 750 rpm (Hara et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The PKH26:MYH10-KD co-cultures 3T3-L1 cells were labeled with 0.5λ PKH26 Fluorescent Cell Linker Kit (Sigma-Aldrich); the GFP cells were added after one day.
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Synaptic varicosities were labeled with primary antibody for vesicular glutamate transporter 1 (vGluT1; 1:2000, 8 days incubation, Guinea Pig, MilliPore Biosciece Research Reagent ...
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bioRxiv - Neuroscience 2021Quote: ... The DIG-labeled probes were hybridized to the membrane in Roche DIG Easy Hyb hybridization buffer (Millipore Sigma cat# 11603558001) at 49°C overnight ...
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bioRxiv - Biophysics 2020Quote: ... Cells were hybridized with labeled DNA probes (Supplementary Table S2) in the FISH Hybridization buffer (10% dextran sulfate (Sigma D8906) and 10% formamide in 2x SSC ...
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bioRxiv - Cancer Biology 2022Quote: ... were coated with fluorescently labeled gelatin as previously described (31) or with 50 μg/ml poly-L-lysine (Sigma-Aldrich) for 20 min and let to air dry ...
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bioRxiv - Microbiology 2022Quote: ... fumigatus was grown for 5 days at 37 °C and conidia were harvested and labeled fluorescein isothiocyanate (FITC; Sigma-Aldrich) or calcofluor white (CFW ...
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bioRxiv - Cancer Biology 2022Quote: ... EV were labeled with a PKH67 dye labeling kit-green (or PKH26 dye labeling kit-red) following the manufacturer’s instructions (Sigma-Aldrich). Briefly ...
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bioRxiv - Bioengineering 2022Quote: Cy5-HA cryogels were synthesized with low- and high-DOS HA-Tz and incubated in 1mL of FITC-labeled 10µM diameter melamine resin micro particles (Sigma Aldrich) at 0.29mg/mL concentration on a rocker at room temperature overnight ...
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bioRxiv - Microbiology 2022Quote: ... Two bottles from each depth were labeled with 1mM Sodium bicarbonate-13C and 1mM Ammonium-15N chloride (Sigma-Aldrich, USA) and all 3 bottles (2 labelled and 1 control ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled samples were washed 3 times in PBT (10 min each) and incubated with DAPI (Sigma, cat. N. D9542) (5μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... OFC β-endorphin sections were co-labeled with the primary antibody for goat anti parvalbumin (PV+) (1:1000, SIGMA, SAB2500752), a marker for a cortical interneuron subtype ...
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bioRxiv - Immunology 2020Quote: ... Cells were then labeled for 5 hours at 4C in 50 μL HBSS with 5 mg/mL bovine serum albumin (BSA, Sigma) with 10 μg/mL wheat-germ agglutinin (WGA ...
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bioRxiv - Bioengineering 2021Quote: ... particles were first labeled with AlexaFluor™ 568 streptavidin and suspended in PBS containing 2 mg/mL fluorescein isothiocyanate-dextran (500,000 MW, Sigma). For 35 and 55 µm nanovials the same procedure was used except the nanovials were first diluted 10-fold to reduce fraction of aggregates formed.
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bioRxiv - Biochemistry 2019Quote: ... Pure tubulin and BSA were labeled with commercial NHS-conjugated dyes (Cy5 or Alexa568) according to supplier’s instructions (Sigma: GEPA150101). A similar method was used to conjugate biotin to tubulin (EZ-Link™ NHS-PEG4-Biotin ...
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bioRxiv - Developmental Biology 2019Quote: ... Digoxigenin (DIG)-labeled RNA probes were prepared by in vitro transcription with linearized constructs using DIG RNA Labeling Kit (SP6/T7) (Sigma). Embryos were fixed in 4% PFA in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were labeled in media for 10 minutes with 500 µM 4-thiouridine (4sU, Sigma-Aldrich, St. Louis, MO, USA.). RNA extraction was performed with TRIzol (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were stained with methylene blue and hybridized to 32P radio-labeled DNA probes (DecaLabel, ThermoFischer) in PerfectHyb™ (Sigma) over night at 65 ºC ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were then labeled in a solution of anti-NeuN antibody conjugated to Alexa Fluor 488 (A60, Millipore, 1/1000) and 0.1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were cultured in complete medium with 1 μg/mL doxycycline for 48 hours labeled with 10 μM EdU (Sigma) for 1 hour prior to harvesting.
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bioRxiv - Molecular Biology 2021Quote: ... The resulting lysates were incubated with a 23-bp customized biotin-labeled DNA substrate containing an O6-methylguanine next to the PstI cleavage site (Sigma) for 2 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Effector (TILs) and target cells (autologous tumor cell lines) were labeled respectively with 1μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) according to the manufacturer’s protocol and loaded on the chip array ...
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bioRxiv - Molecular Biology 2022Quote: ... 32P-labeled probes that reverse complement to the targeted miRNAs were hybridized with membrane in PerfectHyb(tm) Plus Hybridization Buffer (Sigma) overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Ltk-/- and DKO mice were labeled in vivo by intraperitoneal injections with BrdU (150 µg/g body weight, Sigma-Aldrich,). In some experiments PPP (20 mg/kg body weight ...
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bioRxiv - Microbiology 2023Quote: ... Parasites attached to coverslips were fixed in 4% paraformaldehyde at room temperature for 20 min and labeled with wheat germ agglutinin (WGA) lectin from Triticum vulgaris conjugated with FITC (Sigma). To this end ...
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bioRxiv - Microbiology 2022Quote: ... The cell membrane and nuclei of the midgut tissue were labeled for 30 min with FM1-43 (5 μg/ml) (Sigma) and DAPI (0.5 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Polar metabolites were resuspended in 170 µL of 100% methanol containing 13C-15N labeled amino acids (30 µM, 767964, Sigma). UHPLC normal phase chromatography was performed using an Agilent 1290 LC stack ...
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bioRxiv - Biophysics 2022Quote: ... 2H2O M9 media containing 1 g/L 15NH4Cl and 0.5 g/L 2H/15N-labeled ISOGRO (Millipore-Sigma, Burlington, MA). In both cases ...
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bioRxiv - Biophysics 2022Quote: ... 2H2O M9 media containing 1 g/L 15NH4Cl and 0.5 g/L 2H/15N-labeled ISOGRO (Millipore-Sigma, Burlington, MA). In both cases ...
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bioRxiv - Biophysics 2024Quote: ... Fluorecent probe Atto488-DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto-488) was purchased from Sigma-Aldrich (international). Sucrose ...
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bioRxiv - Immunology 2024Quote: ... Infected erythrocytes were labeled with a membrane dye using the PKH26R Red Fluorescent Cell Linker Midi Kit (Sigma #MIDI26-1KT) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich), 0.2 mg/ml glucose oxidase (Merck) ...
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bioRxiv - Biochemistry 2024Quote: ... 15N] triple-labeled proteins for NMR assignments at pH 5.0 were expressed in M9 minimal medium prepared using 100% D2O (Sigma-Aldrich) as solvent (M9 D2O medium) ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa 488-labeled phalloidin was dissolved in methanol to yield a 40X 6.6 μM stock solution whereas DAPI dihydrochloride (Sigma-Aldrich) was dissolved in UPW to yield a 100X 250 μG mL-1 stock solution ...
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bioRxiv - Immunology 2023Quote: ... Alkaline phosphatase (AP)-labeled goat anti-human IgM (μ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA), AP-labeled goat anti-human IgG (γ-chain specific ...
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Plant Biology 2023Quote: ... then resuspended in 100% MeOH containing an internal standard mix of isotopically labeled compounds (∼15 µM average of 5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 10 µg/mL 13C-trehalose ...
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bioRxiv - Genetics 2023Quote: ... the isolated cells were characterized by flow cytometry using the SMC-specific FITC-labeled anti-α-SMA antibody (Sigma, F3777) and an isotype control (mouse IgG2a-FITC ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 DL of the supernatants containing 32P labeled intracellular nucleotides were resolved on a 20 x 20 cm plastic PEI cellulose F TLC plate (Millipore) using 1.5 M KH2PO4 pH 3.4 as a solvent ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Biochemistry 2022Quote: ... N-termini were labeled with light and heavy formaldehyde (12CH2O in H2O, and13CD2O in D2O (252549 and 596388, Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2023Quote: ... 12-mer Atto565-labeled or Atto647N-labeled secondary imaging oligos were diluted to 20 nM in hybridization buffer (5% ethylene carbonate (EC, Cat.# E26258, Sigma) in 2XSSC/0.2% tween) ...
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bioRxiv - Biochemistry 2023Quote: Cell lysates from cardiomyocytes treated with 10 or 50 µM H2O2 and controls were labeled with MAL-PEG (5kDa methoxy polyethylene glycol maleimide, Sigma) following a slightly modified version of a previously published procedure (15) ...