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Citations for Millipore Sigma :
551 - 600 of 7705 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... P2 virus was then harvested by pelleting cells by centrifugation at 4000g and decanting into 5% FBS (Sigma), and then stored in the dark at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the virus-containing supernatant was centrifuged 500 x g for 5min and filtered with 0.45um PES filters (Millipore), followed by ultracentrifugation (25,000rpm for 2h) ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Genetics 2020Quote: ... 10 μL of each virus was diluted in growth media containing polybrene (10 μg/mL; Sigma-Aldrich, USA) then mixed with cells ...
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bioRxiv - Genomics 2021Quote: ... We filtered and concentrated the virus in PBS using Amicon Ultra-15 centrifugation filters (Millipore, Burlington, MA; #UFC905024). The viral titer was measured with the AAVpro(R ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was passaged twice in Vero E6 cell line (ATCC) maintained in DMEM High Glucose (Sigma-Aldrich) supplemented with 2% heat-inactivated Fetal Bovine Serum (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Virus-containing supernatant was collected 48-72 hours post-transfection and filtered through 0.45um PES membrane filter (Millipore). For infection ...
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bioRxiv - Immunology 2020Quote: ... and cells were overlaid with virus growth medium containing Avicel microcrystalline cellulose (final concentration of 1.2%) (Sigma-Aldrich). The plates were then incubated at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated in 1.5 mL virus-containing media supplemented with 0.5 mL fresh media and 0.8 μg/mL polybrene (Millipore Sigma). The 6-well plate was covered with aluminum foil to keep cells in the dark ...
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bioRxiv - Microbiology 2022Quote: ... A27(-) and H3(-) virus stocks were generated in the absence of isopropyl β-d-thiogalactopyranoside (IPTG; Sigma 16758). Plaque forming units /ml and particle counts - calculated from the optical density at 260 nm44 - were determined for each purified recombinant VACV MV stock.
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
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bioRxiv - Pathology 2023Quote: ... Stock virus was produced after three serial passages in Vero E6 cells using Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 2 % fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
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bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
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bioRxiv - Molecular Biology 2021Quote: ... PHM005 wild type and recombinant strains were grown in Marine Broth (MB) Difco 2216 (Sigma-Aldrich) or Marine Agar (MA ...
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bioRxiv - Genetics 2019Quote: ... both WT and Δgh2-1 strains were grown in 2% (w/v) sucrose (Sigma Aldrich, S7903) for 16 h then transferred to 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Other ETEC strains except #400599 (CS14) were cultured in ETEC medium containing 1% Casamino Acids (Sigma) and 0.15% yeast extract (Fisher Bioreagents ...
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bioRxiv - Microbiology 2020Quote: ... aureus strains were routinely grown in Mueller-Hinton (MH) or cation adjusted MH (MHII) medium (Sigma) at 37°C in Erlenmeyer flasks with aeration.
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bioRxiv - Systems Biology 2020Quote: ... TetR ΔarcA and TetR arcA+ (complemented strain) precultures contained 4 ug/ml of tetracycline (Sigma-Aldrich) to maintain selection pressure for resistance ...
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bioRxiv - Microbiology 2021Quote: ... Strains were routinely grown in brain heart infusion (BHI) medium (37 g L−1, Millipore Sigma) supplemented with 5 g L−1 yeast extract (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: Streptococcus mutans strain UA159 was grown in THY (Todd Hewitt Broth + 0.5% yeast extract, Millipore-Sigma) at 37 °C and 5% CO2 and harvested during mid-log phase growth ...
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bioRxiv - Systems Biology 2022Quote: Streptococcus mutans strain UA159 was grown in THY (Todd Hewitt Broth + 0.5% yeast extract, Millipore-Sigma) at 37 °C and 5% CO2 and harvested during mid-log phase growth ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial strains were routinely cultured at 25°C in liquid 50% TSB medium (Sigma-Aldrich, USA) and stored at −80°C in 25% glycerol ...
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bioRxiv - Bioengineering 2019Quote: ... For selection and strain maintenance Synechocystis was cultivated on 0.75% [w/v] agar (Type A, Sigma) plates containing TES-buffered (10 mM ...
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bioRxiv - Systems Biology 2021Quote: ... cerevisiae strain BY4741 was grown in 7 conditions of varying ratios of LiCl (Sigma Aldrich, L9650) and cycloheximide (Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... 15 g/L agar) supplemented for mutant strains with hygromycin B (Sigma-Aldrich; 70 μg/ml) or nourseothricin (Werner Bioagents ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe (strain SHY1058) cultures were labeled with 5 mM 4-thiouracil (4-thioU) (Sigma-Aldrich #440736) for 4 min ...
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bioRxiv - Immunology 2020Quote: ... BCG strains were grown shaking (100 RPM) at 37°C in 7H9 Middlebrook medium (Sigma-Aldrich), complemented with ADC supplement (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... and the mitochondrial GFP reporter strain AfS35 pJW103 (49) were maintained on malt extract (Sigma-Aldrich) agar plates supplemented with 2% (w/v ...
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bioRxiv - Immunology 2021Quote: The ΔyopM mutant strain of Yersinia pseudotuberculosis 32777 was grown overnight in 2X YT media (Sigma) at 25°C with shaking at 200 rpm ...
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bioRxiv - Immunology 2022Quote: ... Strains expressing GFP were grown in media supplemented with 50 μg/mL hygromycin B (Sigma-Aldrich), and strains expressing luxABCDE were grown in media supplemented with 20 μg/mL Zeocin (Thermo).
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bioRxiv - Microbiology 2022Quote: ... pre-cultures of each strain were generated overnight in Mueller-Hinton broth (MHB-II, Sigma, UK). Antibiotic stocks were generated in 50% ethanol at concentrations doubling from 0.25 to 256 mg/L ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with AID degrons were treated with 250 µM auxin (3-indole acetic acid; Sigma-Alrich) in DMSO 30 minutes before harvesting ...
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bioRxiv - Microbiology 2022Quote: ... an overnight culture of the respective bacterial strain was diluted in LB Lennox media (Sigma-Aldrich) and grown to an OD at 600 nm of ≈0.2 ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli strain DH5-α was cultured shaking in Luria-Bertani broth (Lennox) (LB, Sigma-Aldrich) at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... pombe strains grown to an OD600 of 0.5-0.8 were crosslinked using formaldehyde solution (Sigma, F1635) to a final concentration of 1% at RT for 15 min and quenched by addition of glycine (ThermoFisher ...