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Citations for Millipore Sigma :
601 - 650 of 8192 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... A27(-) and H3(-) virus stocks were generated in the absence of isopropyl β-d-thiogalactopyranoside (IPTG; Sigma 16758). Plaque forming units /ml and particle counts - calculated from the optical density at 260 nm44 - were determined for each purified recombinant VACV MV stock.
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
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bioRxiv - Pathology 2023Quote: ... Stock virus was produced after three serial passages in Vero E6 cells using Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 2 % fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
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bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
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bioRxiv - Cell Biology 2024Quote: ... supernatant containing virus particles was harvested and passed through Millex-GP Filter Unit (0.45 μm pore size, Millipore). Viral particles were aliquoted and stored at −80°C ...
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bioRxiv - Microbiology 2024Quote: ... The cells were inoculated with appropriate virus inoculum in the presence of polybrene (Sigma-Aldrich catalog # TR-1003) and cultured for 2 hours in the 37°C/5% CO2 incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... PHM005 wild type and recombinant strains were grown in Marine Broth (MB) Difco 2216 (Sigma-Aldrich) or Marine Agar (MA ...
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bioRxiv - Genetics 2019Quote: ... both WT and Δgh2-1 strains were grown in 2% (w/v) sucrose (Sigma Aldrich, S7903) for 16 h then transferred to 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Other ETEC strains except #400599 (CS14) were cultured in ETEC medium containing 1% Casamino Acids (Sigma) and 0.15% yeast extract (Fisher Bioreagents ...
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bioRxiv - Microbiology 2020Quote: ... aureus strains were routinely grown in Mueller-Hinton (MH) or cation adjusted MH (MHII) medium (Sigma) at 37°C in Erlenmeyer flasks with aeration.
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bioRxiv - Systems Biology 2020Quote: ... TetR ΔarcA and TetR arcA+ (complemented strain) precultures contained 4 ug/ml of tetracycline (Sigma-Aldrich) to maintain selection pressure for resistance ...
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bioRxiv - Microbiology 2021Quote: ... Strains were routinely grown in brain heart infusion (BHI) medium (37 g L−1, Millipore Sigma) supplemented with 5 g L−1 yeast extract (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: Streptococcus mutans strain UA159 was grown in THY (Todd Hewitt Broth + 0.5% yeast extract, Millipore-Sigma) at 37 °C and 5% CO2 and harvested during mid-log phase growth ...
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bioRxiv - Systems Biology 2022Quote: Streptococcus mutans strain UA159 was grown in THY (Todd Hewitt Broth + 0.5% yeast extract, Millipore-Sigma) at 37 °C and 5% CO2 and harvested during mid-log phase growth ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial strains were routinely cultured at 25°C in liquid 50% TSB medium (Sigma-Aldrich, USA) and stored at −80°C in 25% glycerol ...
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bioRxiv - Bioengineering 2019Quote: ... For selection and strain maintenance Synechocystis was cultivated on 0.75% [w/v] agar (Type A, Sigma) plates containing TES-buffered (10 mM ...
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bioRxiv - Systems Biology 2021Quote: ... cerevisiae strain BY4741 was grown in 7 conditions of varying ratios of LiCl (Sigma Aldrich, L9650) and cycloheximide (Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... 15 g/L agar) supplemented for mutant strains with hygromycin B (Sigma-Aldrich; 70 μg/ml) or nourseothricin (Werner Bioagents ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe (strain SHY1058) cultures were labeled with 5 mM 4-thiouracil (4-thioU) (Sigma-Aldrich #440736) for 4 min ...
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bioRxiv - Immunology 2020Quote: ... BCG strains were grown shaking (100 RPM) at 37°C in 7H9 Middlebrook medium (Sigma-Aldrich), complemented with ADC supplement (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... and the mitochondrial GFP reporter strain AfS35 pJW103 (49) were maintained on malt extract (Sigma-Aldrich) agar plates supplemented with 2% (w/v ...
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bioRxiv - Immunology 2021Quote: The ΔyopM mutant strain of Yersinia pseudotuberculosis 32777 was grown overnight in 2X YT media (Sigma) at 25°C with shaking at 200 rpm ...
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bioRxiv - Immunology 2022Quote: ... Strains expressing GFP were grown in media supplemented with 50 μg/mL hygromycin B (Sigma-Aldrich), and strains expressing luxABCDE were grown in media supplemented with 20 μg/mL Zeocin (Thermo).
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bioRxiv - Microbiology 2022Quote: ... pre-cultures of each strain were generated overnight in Mueller-Hinton broth (MHB-II, Sigma, UK). Antibiotic stocks were generated in 50% ethanol at concentrations doubling from 0.25 to 256 mg/L ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with AID degrons were treated with 250 µM auxin (3-indole acetic acid; Sigma-Alrich) in DMSO 30 minutes before harvesting ...
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bioRxiv - Microbiology 2022Quote: ... an overnight culture of the respective bacterial strain was diluted in LB Lennox media (Sigma-Aldrich) and grown to an OD at 600 nm of ≈0.2 ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli strain DH5-α was cultured shaking in Luria-Bertani broth (Lennox) (LB, Sigma-Aldrich) at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... pombe strains grown to an OD600 of 0.5-0.8 were crosslinked using formaldehyde solution (Sigma, F1635) to a final concentration of 1% at RT for 15 min and quenched by addition of glycine (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and commensal bacterial strains were cultivated in 0.5 TSB (15 g.l-1 Tryptic Soy Broth, Sigma) or TY (5 g.l-1 Tryptone ...
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bioRxiv - Systems Biology 2023Quote: ... All strains were grown at 37 °C anaerobically in YBHI media (Acumedia, Bacto, and Sigma-Aldrich) to stationary phase ...
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bioRxiv - Microbiology 2024Quote: ... Both Msmeg or M.tb strains were grown in 7H9 broth containing Congo red (Sigma-Aldrich, USA) and Tween 80 ...
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bioRxiv - Microbiology 2024Quote: ... tuberculosis reference strain H37Rv were cultured in Middlebrook 7H9 broth (Sigma Aldrich, St Louis, MO, USA) supplemented with oleic acid albumin dextrose catalase (OADC ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Target cells were then transduced overnight with the filtered virus in the presence of 8 μg/mL polybrene (Millipore).
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bioRxiv - Genetics 2021Quote: ... The medium was replaced 24 h later and the virus particles were collected and filtered through 0.45 μm filters (Millipore), 24 or 48 h later.
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.1 to 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(- ...
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bioRxiv - Microbiology 2021Quote: ... Virus-containing flocs were then recovered from the sample by filtration onto 90mm 0.2um polycarbonate filters (Isopore, GTTP09030, Millipore) under gentle vacuum ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed and inoculated with virus diluted in infection media (DMEM with 0.3% BSA and 1μg/ml TPCK-treated trypsin (Sigma Aldrich)) and allowed to adsorb for one hour ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... A 2 mL volume of warm virus medium (1X MEM with 100U/100 μg/mL penicillin/streptomycin, 0.3% bovine serum albumin [Sigma]) was added to each well ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...