Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Brdu-ChIP was performed after addition of 20 μM BrdU (5-bromo-2′-deoxyuridine, Sigma Aldrich) directly to HeLa culture medium for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... Senescence was induced by treating cells with 100 µM 5-Bromo-2’-deoxyuridine (Sigma Aldrich, USA) for 48 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... AGS and HUH7 cells pulsed with 10 µM 5-bromo-2′-deoxyuridine (BrdU) (B5002; Sigma-Aldrich) were stained with propidium iodide (P4864 ...
-
bioRxiv - Neuroscience 2023Quote: ... we injected 5-bromo-2′-deoxyuridine (BrdU, 10 mg/ml in saline, 100 mg/kg, Sigma) through an intraperitoneal (i.p. ...
-
bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
-
bioRxiv - Neuroscience 2022Quote: ... and 1% each of Phosphatase Inhibitor Cocktails 2 and 3 (Millipore Sigma #P5726 and #P0044). Cells were scraped ...
-
bioRxiv - Cell Biology 2023Quote: ... was used at 1 µM and ARP 2/3 complex inhibitor - CK666 (182515; Sigma-Aldrich) was used at 100 µM concentration ...
-
bioRxiv - Cell Biology 2023Quote: ... plates were treated with 3-amino-propyltrimethoxysilane (diluted 1:2 with PBS; Sigma-Aldrich/Merck) for 15min at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma, Saint Louis, MO, USA). Particulate was removed by centrifugation of lysates at 13,000 rpm for 10 minutes at 4°C and filtration through 0.45 µm syringe filters and lysates were stored at -80°C until preparation for MIB-MS ...
-
bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
-
bioRxiv - Cell Biology 2024Quote: ... DLD1 cells were treated for 2 h with 0.5 mM 3-indoleacetic acid (Sigma, stock in ethanol). At the time points indicated ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
-
bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
-
bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
-
bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
-
bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
-
bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
-
bioRxiv - Developmental Biology 2020Quote: ... newly hatched or decapsulated tadpoles were immersed in a 500 μM solution of the fluorophore 4-(4-diethylaminostyryl)-1-methylpyridinium iodide (4-di-2-ASP, Sigma D-3418, sigmaaldrich.com) for five minutes at room temperature in the dark ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were fixed with 3.7% paraformaldehyde and washed 3 times with PBS before and after incubation with mouse anti-FLAG M2 primary antibody (Sigma, diluted 1:2000 in PBS with 1% BSA) or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare ...
-
bioRxiv - Plant Biology 2019Quote: ... A ratio of 1:2 and 1:3 (v/v) polyvinylpolypyrrolidone (PVPP, ~110 µm particle size, Sigma-Aldrich, Saint Louis, USA) to ground tissue was used ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were incubated at 37 °C for 30 min to achieve maximum polymerization and then were mixed in a 1:1 volume ratio with pre-warmed PM buffer containing 3 mM MgCl2 and 2% Methylcellulose (MO512, Sigma-Aldrich). Final concentrations of protein ...
-
bioRxiv - Systems Biology 2020Quote: ... 120μL of 1:1 DTNB:GR solution (DTNB: 2mg 5,5’-dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich; Cat#D8130; CAS: 69-78-3) in 3mL KPE ...
-
bioRxiv - Microbiology 2021Quote: ... Allicin was synthesized by oxidation of 3-[(Prop-2-en-1-yl)disulfanyl]prop-1-ene (diallyl disulfide, Sigma-Aldrich, Germany) with peracetic-acid (glacial acetic acid/H2O2 ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (BC-1, BCBL-1) or 20% (BC-2, BC-3, BC-5, BJAB) Serum Plus-II (Sigma-Aldrich, catalog number 14009C ...
-
bioRxiv - Bioengineering 2021Quote: ... the BMOs were placed in an increasing sucrose solution (7.5 % for 1 h, 15 % for 1 h and 30 % O/N; Sigma, S1888) for improved cryoprotection ...
-
bioRxiv - Developmental Biology 2021Quote: 3-4 nl of 2 mg per mL Rhodamine B isothiocyanate-Dextran (70kDa, Sigma) dissolved in PBS and 5 mM HEPES were injected into the sinus venosus of 30 hpf embryos ...
-
bioRxiv - Microbiology 2023Quote: ... 100 µM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine 5’-triphosphate (BzATP) (#B6396, Sigma-Aldrich), 20 µM MCC950 (#inh-mcc ...
-
bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
-
bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
-
bioRxiv - Developmental Biology 2023Quote: ... samples were washed with PBST and incubated at room temperature for 2 h with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) diluted in PBST ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
-
bioRxiv - Biophysics 2024Quote: ... which was prepared with 20 mM HEPES-buffer (2-(4-(2-Hydroxyethyl)-1- piperazinyl)-ethansulfonsäure) (Sigma-Aldrich, ≥99.5 %, H3375) as calcium chloride is not soluble in DPBS-buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... and the resultant supernatants were incubated for 1 h at 4°C with anti-Flag M2 beads (Sigma-Aldrich). The beads were washed three times ...
-
bioRxiv - Neuroscience 2019Quote: ... the sections were incubated for 60 h at 4°C in a solution containing rabbit polyclonal anti-GABA (1:3,000; Sigma) plus mouse monoclonal anti-CB or mouse monoclonal anti-CR (1:1000 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting suspension was centrifuged at 25,000 rpm for 1 h at 4 °C using high-speed centrifuge (Sigma) (Wilson et al. ...
-
bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
-
bioRxiv - Cell Biology 2023Quote: ... Parasites were incubated in 2.5 mL for 1 h at 4 °C with RIPA buffer supplemented with protease inhibitors (Sigma). To remove exogenous biotin ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5 mg ml-1 Hydrocortisone (Sigma #H-0888), 100 ng ml-1 Cholera toxin (Sigma #C-8052) ...
-
bioRxiv - Cell Biology 2022Quote: ... Nocodazole (Sigma, M1404, incubation 5μM for 1 h), MLSA1 (Tocris ...
-
bioRxiv - Immunology 2020Quote: ... blocked for 1 h with rabbit serum (Sigma), then incubated for 1 h with antibody against the FLS marker vimentin (1:50 ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μg/ml hydrocortisone (H-4001; Sigma-Aldrich), 5 μg/ml insulin (128-100 ...
-
bioRxiv - Biophysics 2022Quote: ... 1 μg/mL hydrocortisone (H-4001; Sigma-Aldrich), 5 μg/mL insulin (Cell Applications) ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
-
Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... 7-AAD was used (1:100 in PBS) (Sigma; 9400) for 15 min at RT followed by mounting with MM or Hoechst 33342 (1:1000 in glycerol/PBS ...
-
bioRxiv - Biophysics 2019Quote: ... anti-HA monoclonal (1:1,000; Sigma-Aldrich clone HA-7) or anti-cZP1 N-terminal fragment polyclonal57 (1:3,000) ...