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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were blocked with 10% FBS in PBS for 1 h at RT and incubated overnight at 4°C with a primary anti-γH2AX antibody (05-636, EMD Millipore, 1:500 in 10% FBS-PBS). Following primary antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542) at RT for 2 hours ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Bioengineering 2021Quote: ... we incubated them with suspended lentiviral solutions (MOI 3-5) for 24 h with 4 µg/mL polybrene (Sigma-Aldrich) before we replaced the medium with refresh culture medium ...
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bioRxiv - Plant Biology 2019Quote: ... The total proteins were extracted by using lysis buffer and then incubated for 3 h at 4°C with 20 ul of Anti-HA Affinity Gel (Millipore). The immunoprecipitates were washed five times with lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... chicken anti-neurofilament H (NF; 1:1,000, Millipore AB5539) or rabbit anti-tubulin beta 3 (Tubb3 ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4°C ...
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bioRxiv - Immunology 2024Quote: ... for 1 h and developed with ABTS substrate (Sigma). Absorbance was measured at 405 nm with a spectrophotometer (Molecular Devices ...
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bioRxiv - Cell Biology 2023Quote: ... or Brefeldin A (20 μM, 1 h; Millipore #203729) before fixation and immunofluorescence analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL hydrocortisone (Sigma-Aldrich, cat. H-4001), and Penicillin-Streptomycin (GenDEPOT ...
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bioRxiv - Neuroscience 2024Quote: ... chicken anti-neurofilament H (NF; 1:1,000, Millipore AB5539), and rabbit anti-myosin 7a (Myo7a ...
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bioRxiv - Cell Biology 2019Quote: ... anti-HA (HA-7; 1:40,000 for WB; Cat#H9658, Sigma), rabbit anti-TPI (1:10,000 for WB ...
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bioRxiv - Physiology 2024Quote: ... glucose (1 g/Kg; Cat num. 50-99-7, Sigma-Aldrich) was administered by IP injection and blood glucose measured by glucometer as above at 15- ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM ConA (Sigma, ref. C9705, CAS number 80890-47-7) or 0.1 % DMSO for the vehicle control ...
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bioRxiv - Microbiology 2020Quote: ... probed for 1 h with anti-mouse-HRP-antibody (F1804, Sigma-Aldrich; 1:50,000) diluted in 1×TBS-T buffer containing 3% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000, ABS16, Merck Millipore) followed by incubation with respective HRP-conjugated secondary antibodies (G21040 ...
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bioRxiv - Immunology 2023Quote: ... blocked for 1 h with 200 μL of PBS containing 1% BSA (Sigma-Aldrich), washed 4 times then 100 μL/well of diluted plasma (1:500 for FLS IgG or 1:100 for S1 and S2 IgG and IgG3 ...
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bioRxiv - Neuroscience 2022Quote: Headless larvae (7 dpf) were fixed for three days at 4 °C in EM fixation buffer [2% EM-grade glutaraldehyde (Sigma), 2% PFA (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of DiCre activity when required was by treatment for 2–4 h with 100 nM RAP (Sigma) as described previously (32 ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was incubated for 1.5-2 h at 4 °C with Anti-FLAG M2 affinity gel (Sigma) while gently mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... Rie1-3V5 was IPed at 4°C 2 h using 20 µL of anti-V5-agarose slurry (Sigma). After incubation ...
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bioRxiv - Cell Biology 2019Quote: ... Glut-1 (Supplementary ref. 3) 1:5000 anti-GAPDH (MAB374, Millipore), 1:5000 anti-β-actin (A5441 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were fed by dichloro-dihydro-fluorescein diacetate (DCFH-DA)(D6883, Sigma-Aldrich) for 60 min in fresh DMEM media ...
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bioRxiv - Developmental Biology 2019Quote: ... This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich; 1:10000) for 10 mins at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The nuclei were counter-stained with 1 μg/ml DAPI (4′,6-diamidino-2-phenylindole, Sigma) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000; D9542, Sigma-Aldrich, St Louis, USA), and mounted on glass slides with Mowiol 4-88 (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (1 mg/ml) (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The slides were then stained with 1/1000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 minutes at room temperature and washed twice for 5 minutes in PBS ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cell Biology 2020Quote: ... with 1 μg/mL 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) protease inhibitor (Sigma-Aldrich, Inc.) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2019Quote: ... nuclear labeling was done with DAPI (4’, 6-diamidino-2-phenylindole; 1 μg/mL, Sigma-Aldrich) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 1 μg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) in the dark for 4 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... paraformaldehyde (PFA) and 4-(2-hydroxyethyl)-1-piperazine-ethane-sulfonic acid (HEPES) were purchased from Sigma Chemical (St ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 10.9 μM 4′,6-diamidino-2-phenylindole (DAPI, 1:1000 Sigma). Coverslips were mounted in ProlongGold (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was counterstained with 1 μg/mL DAPI (4’,6’-diamino-2-fenil-indol; Sigma-Aldrich) and mounted onto glass slides with 20 mM Tris pH8 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000).
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated in 4′,6-diamidino-2-phenylindole (1:1000, DAPI, Sigma-Aldrich, Cat # D9542) solution for 10 mins ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min before being incubated with 1:1000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) and 1:200 Phalloidin Alexa-647 (Stratech ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:5,000; Merck Millipore) and covered with Mowiol (Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.4% Triton X-100 and 2% NHS PBS solution during 3 h and then coverslipped with Mowiol (Sigma). After confocal imaging ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 % tetramethylethylenediamine (TEMED, Roth) and 0.005 % 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma) were added to activate the polymerization reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...