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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... intraperitoneal injection of the thymidine analog 5-bromo-2-deoxiurydine (BrDU, Sigma Aldrich) diluted in NaCl 0.9% (10 mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies were prepared at 1:100 in a 1:4 blocking solution (2% BSA (Sigma), 10% normal goat serum (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... counterstained with 5µg of 4’,6-diamidino-2-phenylindole (DAPI – 1:1) (Sigma/Merck, D9542-10mg) to eliminate dead cells before running through the flow cytometer ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Immunology 2022Quote: ... or the ACE2 product Ang (1-7) (Sigma, #A9202; 1 μM) in the same culture conditions described above.
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bioRxiv - Neuroscience 2023Quote: ... 7-benzyloxy-4-trifluoromethylcoumarin (BFC, Sigma), for 90 minutes at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Extracted membranes were separated from insoluble debris by centrifugation (130,000 × g for 1 h at 4 °C) and incubated for 2h with 4 mL of Ni2+-NTA beads (Novagen) equilibrated in buffer III (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: Pseudomonas aeruginosa PQS (2-nonyl-3-hydroxy-4-Quinolone, Sigma, Israel) was dissolved in DMSO to 10 mM concentration ...
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bioRxiv - Microbiology 2022Quote: ... peroxidase-conjugated (1:10.000) for 1 h (Sigma-Aldrich). Before detection of proteins the membranes were washed three times as described before ...
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bioRxiv - Plant Biology 2019Quote: ... The antigen–antibody complexes were visualized using nitrotetrazolium blue chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) buffer (Sigma-Aldrich, St. Louis, Missouri, USA) under standard conditions ...
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bioRxiv - Neuroscience 2022Quote: ... bicuculline (20 μM, acute(0-1h) or 48 h), picrotoxin (10 μM, 1 h), CNQX (10 μM, 1 h) (Sigma), and synthetic Aβ1-42 (Tocris ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour and incubated overnight at 4°C with primary antibody (ATRX: 1:500, H-300 Santa Cruz Biotechnology Inc.; INCENP: 1:3000, Sigma). The following day the membrane was washed and incubated in the appropriate horseradish peroxidase-conjugated secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg/mL 5-fluoro-2′-deoxyuridine + 7 μg/mL uridine (FRDU, F0503, Sigma-Aldrich). Cultured cells were then kept at 37°C and 5% CO2 in an incubator with culture media changes at 48 hours with supplemented media and NGF and FRDU until further experimentation.
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bioRxiv - Synthetic Biology 2021Quote: ... 2 ml of fresh neuron culture media containing 1 μM 4-hydroxytamoxifen (4-OHT; Sigma H6278) was added into each well of neuron culture ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) photoinitiator (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in appropriate secondaries diluted in 2% bovine serum in PBS for 1 h at 20-25°C and counterstained with Hoechst 33257 (1:1000; Sigma). For brain tissue incubated in anti-INO80 antibody (ab118787 ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was gradually dehydrated in resistant glassware with incremental concentrations of tetrahydrofuran (TFH, 50%, overnight; 80%, 1 h and 100%, 2 h, v/v % in distilled water, Sigma-Aldrich). Incubations were performed on a shaker at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... diluted in PBS for 2 h at room temperature and counterstained with 1:5000 Hoestch solution (bisBenzimide H 33258, Sigma-Aldrich). Sections were washed for 20 min in PBS (4 x 5 min rinses) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated over 72 h at 4°C with mouse anti-NeuN (1:300, Millipore, MAB377), rabbit anti-pS6S235/236 (1:300 ...
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bioRxiv - Microbiology 2022Quote: ... were place on the plate and 10 μL of (1, 2, 4 and 9.8 M) H2O2 and (2, 4, 6 M) methyl viologen (Sigma-Aldrich, UK) was dispensed onto each separate disk ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma H0887).
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bioRxiv - Physiology 2019Quote: ... DAPI (4’,6-diamidino-2-phenylidole, 1 μg/mL, Sigma-Aldrich, Israel) was added followed by fluorshield (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Sporozoites were fixed with 4% formalin solution (SIGMA, Cat# HT50-1-2) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (4’,6-diamino-2-phenylindole) (D9542; Sigma; 1 μg/mL). Additionally ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment with HU (Sigma-Aldrich, 0, 0.5, 1, 2, 4, 8 mM) was for 24h ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma H0887). These cells were chosen as an experimental system as previously described (Massacci et al. ...
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bioRxiv - Microbiology 2023Quote: ... 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF) and 1 complete ULTRA protease inhibitor tablet (Sigma-Aldrich)) then clarified by centrifugation at 14,000 x g for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% eosin (H&E) (Sigma). For immunostaining ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 3-methyl-1-butanethiol (Sigma W385808), 1% octanal (Sigma O5608) ...
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bioRxiv - Neuroscience 2022Quote: ... for 3-4 hours> Primary antibodies: Rabbit-α-GluA1 (Sigma-Aldrich AB1504, 1:300) and Mouse-α-bassoon (Abcam ab82958 ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were then washed for 3 × 1 h in 1x PBS with 0.02% NaN3 (Sigma Aldrich – 58032) at RT ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Neuroscience 2019Quote: ... maternal and placental tissues (collected in Experiment 1) using the 2′,7′-dichlorofluorescein diacetate (DCFDA) (Sigma-Aldrich) assay ...
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bioRxiv - Plant Biology 2024Quote: Inflorescences were fixed in a solution of ethanol:water:phormol:acetic acid (10:7:2:1) and then dehydrated and included in Paraplast (Sigma) according to (de Argüeso 1986 ...
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bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich) added fresh] or RIPA buffer [25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
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bioRxiv - Cancer Biology 2021Quote: ... or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437, Sigma Aldrich) for 24 hours prior to collection of cells for RNA extraction.
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bioRxiv - Microbiology 2019Quote: ... 1/200 (v/v) each phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), and 1/100 (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5mM Na3VO4 and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma) and set on ice for 10 minutes prior to sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 3:2:1 in HEK 293T cells (Sigma Aldrich) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 to 3 million cells were cross-linked using 1% formaldehyde (Sigma, F8775) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 from Sigma Aldrich) ...