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Citations for Millipore Sigma :
5751 - 5800 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Proteins from the gels were transferred onto Immobilon-P polyvinylidene fluoride (PVDF, Millipore) membranes for 90 minutes at 70 V ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 μl of Protein A Sepharose CL-4B beads (Sigma; GE17-0780-01) was added in the tube and incubated for and additiona1 hour at 4°C under rotation ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Immunology 2024Quote: ... 5 mg purified RIPR protein fragment was incubated with TEV protease (Sigma/Promega) at a 1:10 v/v ratio overnight at 4 °C on a rolling mixer ...
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bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... the proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (0.45 μm, Millipore) and then incubated with GST-14-3-3 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... and CdtC subunits (within pET-15b protein expression plasmids; #69661, Sigma-Aldrich, USA), each expressed with an amino-terminal polyhistidine fusion peptide ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected by immunoblotting with anti-Cdh1 antibodies (1 µg/ml, Millipore) and anti-GST antibodies (0.5 µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the supernatant were precipitated with 1:4 trichloroacetic acid (Sigma Aldrich). The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated using Amicon Ultra 10K spin columns (Sigma-Aldrich) and dialyzed against hSSB storage buffer.
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were pre-cleared using protein G agarose beads (16-266, Sigma Aldrich). The pre-cleared lysates were incubated with streptavidin-agarose beads (20359 ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were incubated with an equimolar amount of Atto488 maleimide (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and FLAG tagged proteins were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). Elution was carried out with 2X Bolt LDS sample buffer and the samples were blotted onto the PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotin switch-treated protein reduced with 2.5 μL of 0.2 M dithiothreitol (Sigma) for 1 hr at 57°C and the samples cooled before adding 2.5 μL of 0.5M iodoacetamide and incubated in the dark for 45 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were pulled down using anti-FLAG M2 magnetic beads (Sigma) and serially diluted with enzyme buffer before ATP and fluorescein-conjugated PKC substrate peptide (ThermoFisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The eluents were then incubated with S-protein agarose (EMD Millipore, 69704-3) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were extracted from flash-frozen pellets with RIPA buffer (Sigma R0278), supplemented with protease and phosphatase inhibitors (Invitrogen A32961) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein and nucleotide concentration were determined using the Bradford reagent (Sigma Aldrich, Germany) and HPLC ...
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bioRxiv - Neuroscience 2024Quote: ... Immobilized proteins were eluted by thrombin cleavage (thrombin 0.02 UN/µl, Sigma, Germany). The unbound fraction (Sbh) ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Cell Biology 2024Quote: Proteins were resolved in 10% SDS PAGE and transferred to PVDF membrane (Millipore, Burlington ...
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bioRxiv - Immunology 2024Quote: ... The proteins on the gel were then transferred to PVDF membranes (Millipore, IPVH00010), which were blocked in 5% BSA for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a 0.45µm Immobilon-P Membrane (Millipore Sigma cat. IPVH00010) in ice-cold Tris-Glycine Buffer with 20% methanol in a transfer tank either overnight at 10V or 1 hour at 50V ...
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bioRxiv - Molecular Biology 2024Quote: ... Successful protein transfer was confirmed by incubating membranes in Ponceau S solution (Sigma) for 5 minutes with rocking and membranes were blocked in 5% milk in TBS buffer containing 0.1% Tween-20 for 1 hour with gentle rocking ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-glial fibrillary acidic protein (GFAP Cy3-conjugated Sigma-Aldrich, C9205, RRID:AB_476889), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were electrophoretically transferred to an Inmobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore) using the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using a 30 K Amicon Ultra-4 concentrator (Millipore) and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and the plasma membrane protein NaK1 (mouse monoclonal, Millipore, 05-369, 1:2000). Membranes were developed using the ECL Prime Western Blotting System (Amersham Protran ...
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bioRxiv - Neuroscience 2024Quote: ... glial fibrillary acidic protein (GFAP) (1:500; Millipore Sigma; Burlington, MA; Cat#: 345860)] in blocking solution overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... These constructs were used for protein expression in Rosetta (DE3) competent cells (Novagen) cultured in TB as reported previously11,46,47 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein eluate was further concentrated in a 50 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superose 6 Increase 10/300 GL (Cytiva ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Aggregated protein was isolated from the samples using phosphotungstic acid (PTA; Sigma-Aldrich) as described.9 ...
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bioRxiv - Microbiology 2024Quote: ... All proteins were concentrated using a BioMax concentrator (EMD Millipore, Burlington, MA, USA) followed by passage through a 0.2-μm Ultrafree-MC centrifugal filtration device (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... parasitica lysates using the OxyBlot Protein Oxidation Detection kit (Millipore, Billerica, MA, USA). Carbonylated proteins were derivatized with 2,4 Dinitrophenyl hydrazine (DNP ...
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bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal anti-HCoV-OC43 nucleocapsid protein (1:1000, Millipore Sigma, MAB9012). Secondary antibodies coupled to HRP were purchased from molecular probes and Jackson Immuno-research.
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with the enhanced chemiluminescent HRP substrate Crescendo Immobilon (Millipore). Analysis was performed in ImageJ ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1143-1300 of human RTEL1 were amplified by PCR then subcloned between the EcoRI and XhoI restriction sites of pET28 (Novagen). RTEL1 proteins were expressed as 6-histidine-fusions in the E ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Pathology 2022Quote: ... biopsies of human xenograft harvested at the indicated time post infection were digested with 0.4 mg/ml Liberase TL (Sigma Aldrich) in CO2-independent medium for 60 min at 37°C with gentle agitation (100 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Metastases and micro-metastases were assessed using IHC with a human mitochondria antibody (Millipore MAB1273 Anti-Mitochondria clone 113-1).
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...