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Citations for Millipore Sigma :
5701 - 5750 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Dialyzed protein was concentrated using an Amicon Ultra concentrator (30 kDa MWCO, Millipore) and aliquoted into 100 μl amounts and stored at −80 °C.
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-Golgi 58K protein (formimidoyltransferase-cyclodeaminase) mAb (G2404) was from Sigma-Aldrich and used at a dilution of 1:50∼100 ...
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bioRxiv - Cell Biology 2023Quote: Proteins separated with SDS-PAGE were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Membranes were blocked with 5% skim milk in Tris-buffered saline with 0.05% Tween 20 for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-glial-fibrillary acidic protein (GFAP) (Millipore Sigma, AB5804, 1:500) on shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (0.45 µm pore size; Sigma, cat. # GE10600012) for 2.5 h at 400 mA constant ...
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bioRxiv - Neuroscience 2022Quote: ... protein was further concentrated using Amicon Ultra-0.5 mL centrifugal filters (Millipore Sigma) prior to use ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Protein gels were transferred for 60 min to 0.2µm PVDF membranes (ISEQ00010, Sigma) using the semi-dry Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... and protein bands were visualised via coating the membrane in tetramethylbenzidine (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... and precipitated proteins competitively eluted with Flag peptide (150 ng/µl; F4799, Sigma). Lysates were denatured in SDS-PAGE buffer (4x Lämmli buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were concentrated and the protein concentration measured by BCA assay kit (Sigma). ATPase assay ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto PVDF membranes (Immobilon-FL, 0.45 μm pore size, Millipore) following standard procedures ...
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bioRxiv - Developmental Biology 2023Quote: ... for 1 hr at 115 V before transferring to protein membrane (IPV00010, Millipore) at 50 V for 2 hr ...
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bioRxiv - Developmental Biology 2023Quote: Protein was extracted from maternal liver using RIPA buffer (Sigma-Aldrich Corp., R0278). Protein concentration was determined by a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). After ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantifications were normalized with the β-actin signal (A3854; Sigma-Aldrich®).
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bioRxiv - Neuroscience 2023Quote: ... followed by rotating incubation with 20 µl of protein G-Agarose beads (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to nitrocellulose membranes (Millipore). Membranes were probed with a 1:1000 dilution of anti-SARS-CoV-2 S mouse monoclonal primary antibody (1A9 ...
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bioRxiv - Biochemistry 2023Quote: ... and aldehyde concertation was determined using Protein Carbonyl Content Assay Kit (Sigma-Aldrich). Aldehyde concentration was determined to be 4 mol aldehyde per mol protein ...
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bioRxiv - Molecular Biology 2023Quote: ... to which Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) were added for 2hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from the gel were transferred onto a PVDF membrane (Millipore, cat#IPVH00010). The membrane was subsequently blocked and incubated with primary and secondary antibodies ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Plant Biology 2023Quote: ... The protein eluent was concentrated using a 100-kDa cut-off Centricon (Millipore) and subsequently loaded onto a Superose 6 Increase 5/150 GL column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... purified WT or ΔFN2 proteins were incubated with rat tail collagen (EMD Millipore) and then co-IP was carried out as described above.
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bioRxiv - Physiology 2023Quote: ... Proteins were then concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Plant Biology 2024Quote: ... and total protein concentration was measured with Bicinchoninic Acid solution (BCA, Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride membranes (Millipore, Immobilon PSQ, ISEQ00010 or PVH00010) and the membranes were then blocked for 1 hour in 5% non-fat milk in TBS-T and incubated in primary antibodies overnight in 5% BSA in TBS-T at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore) at 200 mA for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein was concentrated by centrifugal ultrafiltration using Amicon Ultra 30 K (Millipore) and quantified spectrophotometrically at 280 nm (mass extinction coefficient E 1% ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were buffer-exchanged against 100 mM ammonium acetate buffer pH 7.4 (Sigma) using Amicon centrifugal filters (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biochemistry 2023Quote: Proteins in 20 µL solution were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in samples were detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich) and image acquisition was performed on a G-Box (Ozyme) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma). 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad) ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was determined using a bicinchoninic acid kit (BCA, Sigma, BCA1-1KT) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM EDTA and proteins were degraded using 100 µg proteinase K (Sigma) and incubation for two hours at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... The purified protein was concentrated to 5 mg/ml by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The purified mini-HA protein was concentrated by Amicon spin filter (Millipore Sigma) and filtered by 0.22 µm centrifuge tube filters (Costar) ...
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bioRxiv - Immunology 2023Quote: ... Protein was then transferred onto a 0.45 µm PVDF membrane (Millipore, Cat. IPFL85R), blocked with 5% non-fat dried milk in TBS plus 0.01% Tween (TBST ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of slurry of Magna ChIP Protein G magnetic beads (Sigma-Aldrich) were washed twice with IP/wash buffer 20 mM Tris HCl pH 7.4 ...