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Citations for Millipore Sigma :
5601 - 5650 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... 1 mM purified protein was mixed with 25% ammonium persulfate (APS) (Sigma-Aldrich) and 6.67 mM [Ru(II)(bpy)3]2+ (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich), and equal amounts of protein were analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride membranes (Millipore, Immobilon PSQ, ISEQ00010 or PVH00010) and the membranes were then blocked for 1 hour in 5% non-fat milk in TBS-T and incubated in primary antibodies overnight in 5% BSA in TBS-T at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich) and centrifuged (12 000 g ...
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bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
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bioRxiv - Immunology 2024Quote: ... 5 mg purified RIPR protein fragment was incubated with TEV protease (Sigma/Promega) at a 1:10 v/v ratio overnight at 4 °C on a rolling mixer ...
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bioRxiv - Plant Biology 2024Quote: ... and total protein concentration was measured with Bicinchoninic Acid solution (BCA, Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM EDTA and proteins were degraded using 100 µg proteinase K (Sigma) and incubation for two hours at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... The purified protein was concentrated to 5 mg/ml by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The purified mini-HA protein was concentrated by Amicon spin filter (Millipore Sigma) and filtered by 0.22 µm centrifuge tube filters (Costar) ...
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bioRxiv - Neuroscience 2024Quote: ... CDK5 was immunoprecipitated (4°C overnight with 2x Protein A agarose (Sigma-Aldrich)) from SCN samples at ZT14 in the dark or after a light pulse (LP. ...
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bioRxiv - Microbiology 2024Quote: ... Fractions containing the protein of interest were concentrated (Amicon Ultracel 30 K, Millipore) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... External mass calibration was performed thanks to SpheriCal Aqua Protein Low kit (Sigma).
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bioRxiv - Molecular Biology 2024Quote: Proteins were transferred from SDS PAGE onto Immobilon-FL PVDF membranes (Millipore Sigma) using a semi-dry transfer apparatus (15V ...
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bioRxiv - Developmental Biology 2023Quote: ... The lysates were pre-cleared using Protein A agarose beads (11134515001, Sigma-Aldrich) at 4 ℃ for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated using a 10 kDa MWCO Amicon concentrator device (Millipore). The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2024Quote: ... The collected proteins were concentrated by an Amicon Ultra-3k centrifugal filter (Millipore), exchanged with PBS supplemented with 100 mM NaCl and 50% glycerol ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were incubated with streptavidin-agarose (16-126; Millipore sigma, Burlington, MA) for one night at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Eluted proteins were buffer exchanged using 50 kDa Amicon centrifugal units (EMD Millipore), resuspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore) at 200 mA for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore) and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences ...
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bioRxiv - Microbiology 2023Quote: ... G9R and A1L proteins were labeled with primary anti-Flag (F1804, Sigma-Aldrich) and anti-HA (26183 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore, Burlington, MA, USA). Incubation with primary antibodies was carried out at 25 °C for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins from the gels were transferred onto Immobilon-P polyvinylidene fluoride (PVDF, Millipore) membranes for 90 minutes at 70 V ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with Protein G agarose beads (EMD Millipore 16-266) for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins in the supernatant were digested with 100 µg/mL proteinase K (Sigma) at 37°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The total protein concentration in the lysates was quantified using Bradford reagent (Sigma), and equal amounts of bacterial proteins were resolved by SDS-PAGE ...
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bioRxiv - Microbiology 2024Quote: ... The total protein content of the samples was quantified using Bradford assay (Sigma). Equal concentrations of protein samples were mixed with 5x Laemmle buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... protein was reduced (2.5 mM dithiothreitol, Sigma-Aldrich; 30 min at 37°C) and alkylated (10 mM iodacetamide ...
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bioRxiv - Biochemistry 2024Quote: ... Purified protein samples were concentrated using Amicon Ultra 10K spin column (Sigma-Aldrich) and dialyzed against Storage buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using Amicon™ Ultra centrifugal filter units (Millipore Sigma) with a molecular cutoff of 10 kDa and stored at −80 °C.
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bioRxiv - Microbiology 2024Quote: ... The refolded proteins were concentrated with Amicon 3 kDa Ultracel filters (EMD Millipore). For GFP-PSB15 purification ...
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bioRxiv - Immunology 2024Quote: ... The proteins were transferred from SDS-PAGE to PVDF membrane (0.2-μm, Millipore) in Tris-Glycine Transfer buffer (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was passed through low-protein binding 0.45-µm syringe filter (Millipore). The lentivirus was purified by two different methods for comparison ...
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bioRxiv - Biochemistry 2023Quote: Proteins in 20 µL solution were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...