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Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Microbiology 2020Quote: The sequences encoding mature VdAve1 and VnAve1 were cloned into pET-15b with an N-terminal His6 tag sequence (Novagen, Madison, WI, USA) (primer sequences ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Cell Biology 2022Quote: ... Strains containing AID tag alleles were treated with final concentrations of 250 µM 3-indole acetic acid (Sigma-Aldrich, St. Louis, MO, USA) in DMSO and buffered with 50 mM potassium phosphate buffer at pH 6.2 for the 30 minutes prior to harvesting or imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed in PBS and placed in a humid chamber to be stained with the primary antibody anti-HA tag at a concentration of 8 μg/mL (anti-rabbit, #H6908, Sigma-Aldrich, Deisenhofen, Germany) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% NaN3) and incubated with primary antibody (monoclonal α-V5-Tag mouse antibody /Elabscience/ and monoclonal α-Flag M2 mouse antibody /Sigma Aldrich/) and secondary antibody (AF488-conjugated goat anti-mouse antibody /Elabscience/) ...
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bioRxiv - Immunology 2019Quote: ... 1 μM oligomycin and 100 mM 2-deoxy-D-glucose (2-DG; Sigma).
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bioRxiv - Cancer Biology 2020Quote: ... SASI_Hs01_00061211, #2; SASI_Hs01_00061214), and PDX1(#1; SASI_Hs01_00108076, #2; SASI_Hs01_00108080) were purchased from Sigma. shRNA-containing plasmids targeting human and mouse RNF125 (Human ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% SDS) supplemented with protease inhibitor and phosphatase inhibitors 2 and 3 (Sigma). Samples were subjected to dounce homogenization ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 % Span80 (Fluka) surfactant and 4 % 2-Hydroxy-2-methylpropiophenone (97%, Sigma-Aldrich) photoinitiator ...
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bioRxiv - Neuroscience 2020Quote: ... BCH (Slc7a5 inhibitor 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid, Sigma-Aldrich) was stored as a 100 mM stock solution in 1N NaOH ...
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bioRxiv - Physiology 2022Quote: ... GSH in deproteinated samples was derivatized with 2-vinylpyridine (Sigma-Aldrich, #13229-2) prior to assessment ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1% (v/v) photo-initiator (2-hydroxy-2-methylpropiophenone, Sigma, Product No.: 405655) in buffer (75 mM Trizma HCl pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: 2 µM mTLR9-ECD was mixed with 2 µM ssDNA oligo (Sigma-Aldrich) in 10 mM MES pH 6 ...
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bioRxiv - Physiology 2020Quote: ... elegans day 2 adult worms were fixed with 2% paraformaldehyde (Sigma Aldrich, P6148) and 1% glutaraldehyde (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 500 µL of 2% 3-aminopropyltriethoxysilane (Sigma; 919-30-2) in 95% ethanol for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml/well overlay [MEM containing 2% FBS and 0.4% Tragacanth (Sigma, Israel)] was added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 2 ml/well overlay [MEM containing 2% FBS and 0.4% Tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (Sigma, SML0601); an irreversible O-Carnitine palmitoyltransferase-1 (CPT1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The ATP regeneration system (2 mM ATP, 2 mM phosphoenolpyruvate [PEP, Sigma-Aldrich, #P7127] ...
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bioRxiv - Bioengineering 2022Quote: ... blocked with 2% Abdil (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) for 1 hour ...
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bioRxiv - Systems Biology 2022Quote: ... Fenofibrate (2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester) (Sigma # F6020) was injected peritoneally (4% DMSO/PBS ...
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bioRxiv - Bioengineering 2024Quote: ... then coated with 1∼2 μg cm−2 laminin (No. L2020, Sigma-Aldrich) for more than 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Sigma-Aldrich), 1.2 mg/ml Sodium Bicarbonate (Sigma-Aldrich/MilliporeSigma ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... 8 ml of binding buffer [PBS-2 mM EDTA-2% BSA (Sigma Aldrich) (w/v)] were added and the mix was passed through a 40 µm filter (Avantor) ...
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bioRxiv - Immunology 2023Quote: ... for 72 h with or without 1 mM 2-desoxyglucose (2-DG, Sigma). Tumor size was assessed every day using a caliper ...
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bioRxiv - Cell Biology 2023Quote: – 5-iodo-2’-deoxyuridine (IdU) and 5-Chloro-2′-deoxyuridine (CldU) (Sigma-Aldrich) were dissolved in sterile DMEM at 2.5mM and 200mM respectively and stored at –20°C ...
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bioRxiv - Cell Biology 2023Quote: ... a 0.1% 2-Hydroxy-2-methylpropiophenone photo-initiator (PI) (Irgacure, Sigma Aldrich®) was prepared using PBS ...
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bioRxiv - Cell Biology 2023Quote: A 100 mM stock of 2-bromopalmitic acid (2-BP, Sigma-Aldrich 248422) or methyl-palmitate (MP ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% of a photo-initiator (2-hydroxy)2-methylpropiophenone (MPP - Sigma-Aldrich #405655), 0.5% ammonium persulfate (APS ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 mM 2-Deoxy-D-glucose (2-DG, D6134-5G, Sigma-Aldrich). Changes in extracellular acidification rate (ECAR ...
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bioRxiv - Microbiology 2024Quote: ... and either 2 mM glutamine (Quality Biological) or 2 mM asparagine (Sigma-Aldrich) as specified for 8 h at the indicated MOI ...
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977; 2% 2-heptanone in mineral oil, Sigma Aldrich product # 537683 ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Developmental Biology 2020Quote: ... the samples were washed 3x for 5min with PBS and incubated with secondary antibodies (1:1000) and Hoechst (Sigma, 1mg/ml stock, 1:1000) diluted in staining solution for 2hrs at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% dry milk powder in PBS with 0.1% Tween and incubated with anti-N1ICD (Val1744, CST, 1:1000), anti-Notch1 (D1E11, CST, 1:1000) and anti-β-actin (Sigma-Aldrich, 1:4000) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking buffer at the following dilutions and incubated on tissue sections at 4°C overnight: Calb1 (Swant, CB-38a, 1:1000) and Pvalb (Millipore-Sigma, MAB1572, 1:1000). Sections were then washed three times PBS and incubated in anti-mouse or anti-rabbit fluorescently conjugated secondary antibodies (Invitrogen Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Flag antibody (1:1000, F7425) was obtained from Sigma-Aldrich. HA.11 Epitope Tag Antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with mouse anti-m6A antibody (1:1000, Millipore # MABE1006) at 4°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-PLS3 (1:1000; #SAB2700266, Millipore-Sigma, Burlington, MA) primary antibodies followed by anti-mouse (#A4416 ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-PLS3 (1:1000; #SAB2700266, Millipore-Sigma, Burlington, MA) primary antibodies followed by anti-mouse (#A4416 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies recognizing β-catenin (Sigma, MA1-301, 1:1000), α-SMA (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 U/ml LIF (Catalog No. ESG1107, Millipore, Darmstadt, Germany), in either DMEM-high glucose medium (Catalog No ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal anti-PCSK1 (PC1/3) (1:1000, Sigma, #SAB1100415), rabbit polyclonal anti-TSSC1/EIPR1 (1:1000 ...