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5751 - 5800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019

• Co-translational biogenesis of lipid droplet integral membrane proteins

  Pawel Leznicki, et al., bioRxiv - Biochemistry 2021

  Quote: ... rabbit anti-FLAG (Sigma Aldrich, cat. number F7425, 1:1000), anti-AUP1 (Bethyl Laboratories ...
• New cell biological explanations for kinesin-linked axon degeneration

  Yu-Ting Liew, et al., bioRxiv - Cell Biology 2021

  Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively ...
• The origin of mouse extraembryonic endoderm stem cell lines

  Jiangwei Lin, bioRxiv - Developmental Biology 2021

  Quote: ... and 1000 IU/ml leukemia inhibitory factor (LIF) (Millipore #ESG1107). Wells were coated with gelatin (Specialty Media #ES-006-B ...
• A novel membrane protein Hoka regulates septate junction organization and stem cell homeostasis in the Drosophila gut

  Yasushi Izumi, Kyoko Furuse, Mikio Furuse, bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-PH3 (06-570; Millipore, Darmstadt, Germany; 1:1000), rabbit anti-dpERK (4370 ...
• Metal transporter SLC39A14/ZIP14 modulates regulation between the gut microbiome and host metabolism

  Trista L. Thorn, et al., bioRxiv - Physiology 2021

  Quote: ... 0.2% b-mercaptoethanol and 1000 units/ml of lyticase (Sigma), incubated at 37°C for 30 min to disrupt fungal cells as described 58,59 prior to processing through the QIAamp Fast DNA Stool Mini Kit (Qiagen) ...
• A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A

  Matthew Simon, et al., bioRxiv - Molecular Biology 2021

  Quote: ... Lamin A/C-1:1000 (Abcam ab108595, Millipore 05-714), γH2AX (Millipore 05-636) ...
• Mllt11 regulates the migration and neurite outgrowth of cortical projection neurons during development

  Danielle Stanton-Turcotte, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and mouse anti-acetylated α-tubulin (1:1000, Millipore Sigma).
• Long-lasting tagging of neurons activated by seizures or cocaine administration in Egr1-CreER^T2 transgenic mice

  Sophie Longueville, et al., bioRxiv - Neuroscience 2020

  Quote: ... glial fibrillary acidic protein (GFAP, 1:1000, #SAB4100002, Sigma-Aldrich), NeuN (1:500 ...
• Broad influence of mutant ataxin-3 on the proteome of the adult brain, young neurons, and axons reveals central molecular processes and biomarkers in SCA3/MJD using knock-in mouse model

  Kalina Wiatr, et al., bioRxiv - Neuroscience 2021

  Quote: ... nuclear stain at 1:1000 and embedded in Fluoroshield (Sigma) mounting medium.
• Telomere relocalization to the nuclear pore complex in response to replication stress

  Alexandra M Pinzaru, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-c-Myc antibody clone 9E10 (Millipore, 1:1000 dilution), anti-HA.11 clone 16B12 (Biolegend ...
• Single-cell profiling identifies ACE⁺ granuloma macrophages as a non-permissive niche for intracellular bacteria during persistent Salmonella infection

  Trung H. M. Pham, et al., bioRxiv - Immunology 2022

  Quote: ... and 1000 U/ml polymyxin B (Millipore Sigma P4932-5MU). Bone marrow engraftment was assessed ~8 weeks after transplantation ...
• A Sterol-PI(4)P exchanger controls the Tel1/ATM axis of the DNA Damage Response

  Sara Ovejero, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-total CHK2 (05-649, Millipore, WB at 1/1000), anti-total ATM (GTX132147 ...
• EWSR1 prevents the induction of aneuploidy by regulating the localization of Aurora B at inner centromere

  Haeyoung Kim, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse anti-β-actin antibody (1:1000 dilution, Sigma, #A2228); chicken anti-mCherry antibody (1:1000 dilution ...
• Receptor interacting protein kinase-3 mediates both myopathy and cardiomyopathy in preclinical animal models of Duchenne muscular dystrophy

  Maximilien Bencze, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit antibody to mouse pan-Laminin (Sigma, L9393, 1/1000), Phospho-RIPK1 (Ser161 ...
• Densified Collagen Tubular Grafts for Human Tissue Replacement and Disease Modelling Applications

  Alexander W. Justin, et al., bioRxiv - Bioengineering 2022

  Quote: ... a HRP-conjugated secondary antibody was added (1:1000, Sigma) in TBS containing 10 % goat serum ...
• Chimeric 3D-gastruloids – a versatile tool for studies of mammalian peri-gastrulation development

  Alexandra E. Wehmeyer, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Leukemia inhibitory factor (ESGRO LIF, Merck Millipore, 1000 U/ml), and 2i ...
• Engineering High Throughput Screening Platforms of Cervical Cancer

  Ines A Cadena, et al., bioRxiv - Bioengineering 2022

  Quote: ... with thrombin from human plasma (1000 U/mL, Sigma-Aldrich) in PBS for a final concentration of 50 U/mL thrombin and 0.5 ...
• SWIP mediates retromer-independent membrane recruitment of the WASH complex

  V. Dostál, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-CCDC53 (Millipore, cat. no. ABT69, 1:1000 for WB), anti-EEA1 (Cell Signaling ...
• Genome-wide CRISPR/Cas9 Screens Reveal Shared and Bespoke Mechanisms of Resistance to SHP2 inhibition

  Wei Wei, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Pan-RAS antibody (Ab-3, 1:1000) was from Millipore. Antibodies against KRAS (12063-1-AP ...
• HD-PTP/PTPN23 hypomorphic mice display lipodystrophy

  Brian A. Davies, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-actin rabbit polyclonal antibody (1:1000, A2066, Sigma Aldrich), anti-β-actin mouse monoclonal antibody (1:10000 ...
• Segregation of pathways leading to pexophagy

  Francesco G. Barone, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-PMP70 (Sigma, SAB4200181, 1:1000 WB, 1:250 IF), mouse anti-actin (ProteinTech ...
• CasTuner: a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression

  Gemma Noviello, et al., bioRxiv - Genetics 2022

  Quote: ... supplemented with 1000 U/ml leukaemia inhibitory factor (LIF, Millipore) only for all experiment performed in 1.8XX mESCs or supplemented with LIF and 2i (3 μM Gsk3 inhibitor CT-99021 ...
• SARS-CoV-2 Omicron spike H655Y mutation is responsible for enhancement of the endosomal entry pathway and reduction of cell surface entry pathways

  Mizuki Yamamoto, et al., bioRxiv - Microbiology 2022

  Quote: ... mouse anti-tubulin (1:1000, CP06, Millipore, Billerica, MA, USA), and mouse anti-VSVM (1:1000 ...
• The PTEX pore component EXP2 is important for intrahepatic development during the Plasmodium liver stage

  Tahir Hussain, et al., bioRxiv - Microbiology 2022

  Quote: ... mouse anti-FLAG monoclonal antibody M2 (Sigma; 1:1000 IFA); goat polyclonal anti-UIS4 (LS Biolabs LS-C204260 ...
• An in vitro microfluidic culture device for peripheral neurotoxicity prediction at low concentration based on deep learning

  Xiaobo Han, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... mouse anti-β-tubulin III (1:1000, T8578, Sigma– Aldrich) at 4°C for 24 h ...
• CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes

  Mable Lam, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-NeuN (Clone A60, EMD Millipore MAB377, 1:1000), rabbit anti-Caspr (Abcam ab34151 ...
• Neuron-specific protein expression at striatal excitatory synapses

  Yi-Zhi Wang, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-Prkaca (1:1000, Millipore Sigma, Cat # HPA071185, RRID:AB_2686356), rabbit anti-GluA2 (1:1000 ...
• Adult-specific Reelin expression alters striatal neuronal organization. Implications for neuropsychiatric disorders

  Mònica Pardo, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-Tyrosine Hydroxilase (TH, 1:1000, Rabbit, AB152, Merck Millipore). Sections were washed with PBS-T and then incubated for 2 h at RT with biotinylated secondary antibody (1:200 ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-GFAP (1:1000; Millipore-Sigma #G9269; RRID #AB_477035), mouse biotin-anti-GFAP (1:1000 ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-NeuN (1:1000; Millipore-Sigma #MAB377; RRID # AB_2298772), rabbit anti-Iba1 (1:1000 ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-NeuN (1:1000; Millipore-Sigma #ABN78; RRID #AB_10807945), mouse anti-NeuN (1:1000 ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-GFAP (1:1000; Millipore-Sigma #G9269; RRID #AB_477035), mouse biotin-anti-GFAP (1:1000 ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-NeuN (1:1000; Millipore-Sigma #ABN78; RRID #AB_10807945), mouse anti-NeuN (1:1000 ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-NeuN (1:1000; Millipore-Sigma #MAB377; RRID # AB_2298772), rabbit anti-Iba1 (1:1000 ...
• HIV integration in the human brain is linked to microglial activation and 3D genome remodeling

  Amara L. Plaza-Jennings, et al., bioRxiv - Neuroscience 2022

  Quote: ... nuclei were stained with NeuN-488 (MAB377X, Millipore; 1:1000). All antibodies were incubated for 1 hour at 4°C protected from light ...
• Temporal changes in the microglial proteome of male and female mice after a diffuse brain injury using label-free quantitative proteomics

  Yasmine V. Doust, et al., bioRxiv - Neuroscience 2022

  Quote: ... and rabbit anti-MBP (1:1000; Sigma, cat no. HPA049222) diluted in 1% block solution and 0.01M PBS overnight at 4°C ...
• Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the Combi-CRISPR technology

  Natsuki Matsushita, et al., bioRxiv - Neuroscience 2022

  Quote: ... and mouse anti-Cre antibody (1:1000; Millipore, Billerica, MA) or goat anti-GFP antibody (1 μg/mL ...
• Quantitation of FGFR3 signaling via GRB2 recruitment on micropatterned surfaces

  Ingrid Hartl, et al., bioRxiv - Molecular Biology 2022

  Quote: ... 1000 U/ml penicillin-streptomycin (all from Sigma-Aldrich, USA) in a humidified atmosphere at 37 °C and 5 % CO2 ...
• Distinct Neurexin-Cerebellin Complexes Control AMPA- and NMDA-Receptor Responses in a Circuit-Dependent Manner

  Jinye Dai, et al., bioRxiv - Neuroscience 2022

  Quote: ... and anti-vGluT1 guinea pig (1:1000; Millipore; Cat# AB5905).
• Astrocytes regulate inhibition in Fragile X Syndrome

  Maham Rais, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-PV (1:1000; Millipore, catalog #MAB1572, RRID: AB_2174013); rabbit anti-ErbB4 (1:1000 ...
• Training-Induced Circuit-Specific Excitatory Synaptogenesis is Required for Effort Control

  Francesco Paolo Ulloa Severino, et al., bioRxiv - Neuroscience 2022

  Quote: ... with a primary antibody against GFP (1:1000; Millipore, AB16901). In addition ...
• MOV10 facilitates messenger RNA decay in an N6-methyladenosine (m⁶A) dependent manner to maintain the mouse embryonic stem cells state

  Majid Mehravar, et al., bioRxiv - Biochemistry 2022

  Quote: ... supplemented with Protease Inhibitor Cocktail (1:1000 dilution, P8340, Sigma), 80 U of RNase inhibitor (20 U/µL ...
• Nucleic acid receptor ligands improve vaccination efficacy against the filarial nematode Litomosoides sigmodontis

  Johanna F. Scheunemann, et al., bioRxiv - Immunology 2022

  Quote: ... 1:1000 rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) at 4°C ...
• Slow integrin-dependent migration organizes networks of tissue-resident mast cells

  Sarah K. Bambach, et al., bioRxiv - Cell Biology 2022

  Quote: ... Primary antibodies against pan-talin protein (1:1000, Sigma-Aldrich) and actin control (1:2000 ...
• Variability in HIV-1 Transmitted/Founder Virus Susceptibility to Combined APOBEC3F and APOBEC3G Host Restriction

  Amit Gaba, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1000), rabbit anti-Vif antibody (Cat# 809 ...
• Lysine Demethylase 4A is a Centrosome Associated Protein Required for Centrosome Integrity and Genomic Stability

  Pratim Chowdhury, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-SETD2 (1:1000, Sigma Aldrich Cat. # HPA-042451-100), anti-H3K36me3 (1:1000 ...
• The DBD-α4 helix of EWS::FLI is required for GGAA microsatellite binding that underlies genome regulation in Ewing sarcoma

  Ariunaa Bayanjargal, et al., bioRxiv - Cancer Biology 2024

  Quote: ... monoclonal anti-FLAG M2 mouse (Sigma F1804-200UG, 1:1000); anti--Tubulin [DM1A] mouse (Abcam ab7291 ...
• Angiogenin regulates mitochondrial stress and function via tRNA-derived fragments generation and impacting tRNA modifications

  Shadi Al-Mesitef, et al., bioRxiv - Molecular Biology 2024

  Quote: FLAG-M2 antibody (1:1000 dilution, Sigma Aldrich, Cat# F3165).
• Nephronophthisis-associated FBW7 mediates cyst-dependent decline of renal function in ADPKD

  Maulin Mukeshchandra Patel, et al., bioRxiv - Genetics 2024

  Quote: ... Other antibodies used were SOX9 (1:1000, Sigma-Aldrich AB5535), TMEM237 (1:1000 ...
• Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG

  Xiaoyun Ding, et al., bioRxiv - Neuroscience 2024

  Quote: ... and Calbindin D-28K (Millipore Cat# AB1778, RRID:AB_2068336, 1:1000). Chicken polyclonal antibodies against GFAP (Abcam Cat# ab134436 ...