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Citations for Millipore Sigma :
5501 - 5550 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CSR to IgG3 and IgG1 was induced in T cell independent manner by exposure to 5 μg/ml of Lipopolysaccharide (Escherichia Coli LPS, 055:B5, Sigma) or LPS+rIL-4 ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Molecular Biology 2019Quote: ... while LMNB1-CT was expressed in Escherichia coli B834 (DE3) cultured in LB medium with 50 μg/mL of kanamycin (Sigma). EHMT1 (2IGQ ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was then cut in half with a clean razor and each piece was placed in a 6-well plate with 10 ml of ASW and lipopolysaccharide (LPS) from Escherichia coli 0111:B4 (Sigma) at a final concentration of μg/ml (or an equal volume of sterile diH2O ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 50 mg l-1 kanamycin and 34 mg l-1 chloramphenicol was used for growth of Escherichia coli Rosetta (Novagen) to induce protein production ...
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bioRxiv - Biochemistry 2021Quote: ... wild-type (WT) or D274N were individually expressed from a pET30-TEV vector in BL21 (DE3) pLysS Escherichia coli (Novagen), with protein expression being induced with 0.4 mM IPTG for 18 h at 18 °C ...
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bioRxiv - Genomics 2022Quote: ... Fifteen individuals were intra-abdominally injected with 0.25 mg of LPS (Escherichia coli O55:B5; Sigma-Aldrich, cat. no. L2880) dissolved in 50 μl of Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2021Quote: The pOP3MT plasmid containing the sequence of the mature form of SKD3 was transformed into Escherichia coli BL21(DE3) (Millipore-Sigma). Cells were cultured in 2 × 5 ml LB broth with 100 μ g/ml ampicillin for 16 hours at 30 °C with shaking ...
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bioRxiv - Microbiology 2020Quote: ... coli JKe201 [28] was cultured in the presence of 100 µM of diamino pimilic acid (DAP) (Sigma Aldrich D1377-10G). Salmonella enterica serovar Typhimurium SL1344 was cultured in LB in the presence of 90 µg/ml streptomycin (Sigma-Aldrich S9137-100G) ...
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bioRxiv - Molecular Biology 2021Quote: ... and TniQ were expressed in Escherichia coli BL21(DE3) while TnsC was expressed in Rosetta™(DE3)pLysS (Novagen: #70956) containing a pLysS-tRNA plasmid ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Microbiology 2022Quote: ... coli and inserted between the BamHI and XhoI sites in the multicloning site of the pET21a plasmid (Novagen, Madison, WI). The recombinant protein ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells diluted in M63 minimal medium as above were incubated with or without 1.5 mM hydrogen peroxide (Sigma-Aldrich) or 60 µg/mL phleomycin (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: ... coli was grown overnight at 37°C and 200 rpm in LB broth (L7275-500TAB, Sigma-Aldrich, St. Louis, MO) from a colony scrape taken off an LB agar plate ...
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bioRxiv - Neuroscience 2022Quote: WT and TG-G2019S mice were exposed to intraperitoneal (ip) injections of a low dose of lipopolysaccharide LPS (0,1 mg kg−1 Escherichia coli serotype O111:B4) (Sigma-Aldrich), administrated twice a week for 12 weeks (Supplementary Figure 1) ...
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bioRxiv - Immunology 2022Quote: ... On day 7 differentiated macrophages were divided into six-well plates at a density of 0.8×106 cells/well in a total volume of 3 ml in MGM diluted to contain 10% L929 cell-conditioned medium (MGM 10/10) and primed for ∼18hrs with 100 ng/ml O26:B6 Escherichia coli LPS (Sigma).
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bioRxiv - Immunology 2024Quote: ... was diluted in pre-warmed complete RPMI to a final concentration of 0.5ug/ml and 1.0ug/mL and was applied for 4 hours prior to lipopolysaccharide (LPS, from Escherichia coli O111:B4, Sigma-Aldrich) treatment ...
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The SMC1A or SMC3 HDs-coding plasmids were respectively co-transformed with the RAD21C- or RAD21N-coding plasmids into chemically competent Escherichia coli BL21(DE3) cells (Novagen). Co-transformed cells were selected using the appropriate antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Molecular Biology 2023Quote: ... coli MC1061 strain and Luria-Bertani (LB)-agar supplemented with ampicillin (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA) for selection ...
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bioRxiv - Biochemistry 2023Quote: ... Bacteria Escherichia coli and Bacillus subtilis strains were grown in Luria Bertani (LB) medium (Sigma, St. Louis, MO, United States) at 37°C and 180 rpm ...
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bioRxiv - Bioengineering 2023Quote: ... tissue cultures were treated with bacterial lipopolysaccharide (LPS from Escherichia coli O111:B4, #L4391, Sigma-Aldrich, UK, 1 µg/mL) for 3 days inside the incubator and imaged before and after 3 days of treatment as previously described.51
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bioRxiv - Biochemistry 2023Quote: ... Both constructs employed a linker with a rhinovirus 3C protease (PreScission Protease) recognition site and were transformed into Escherichia coli Rosetta2(DE3) cells (Novagen) for overexpression ...
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bioRxiv - Cell Biology 2023Quote: ... The NF-κB activation inhibitor BAY 11-7085 and lipopolysaccharides (LPS) from Escherichia coli O55:B5 were from Sigma Aldrich. Recombinant human vascular endothelial growth factor-165 (VEGF ...
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bioRxiv - Neuroscience 2023Quote: ... Cells at passage 9 to 15 were treated with LPS (from Escherichia coli O55:B5, 100 ng/mL, Sigma #L6529) or with IFNγ (100 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The ligated pTrcHisB/PRNP and pTrcHisB/Prnp constructs were used to transform the Escherichia coli host strain BL21(DE3) (Novagen), genotype F′ ompT hsdSB (rB-mB- ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides from Escherichia coli 055: B5 (L2880) and Phorbol 12-myristate 13-acetate (PMA) (P1585) were obtained from Sigma-Aldrich. Yoda1 (5586 ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged PRDX6 from rat (gene cloned into pET15b NdeI and XhoI restriction sites) as produced in the Escherichia coli strain Origami (DE3) (Novagen). 20 µL of Prdx6- Pet15b transformed Origami (DE3 ...
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bioRxiv - Immunology 2024Quote: ... the cells were treated with either vehicle or 500 ng/mL of LPS from Escherichia coli O55:B5 (Sigma-Aldrich). Pellets and supernatants were collected and immediately flash frozen at one hour for pellet RNA expression and supernatant analyses ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hour at room temperature and then incubated in anti-NALCN (1: 1000, Alomone Labs, Israel, ASC-022; RRID: AB_11120881) and anti-TH (1:1000, Millipore, USA, MAB318; RRID: AB_2201528) for 18 hours at 4°C ...
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bioRxiv - Genetics 2022Quote: ... in PBS and incubated with rabbit polyclonal vault antibodies (N2-B15, provided by Leonard Rome, dilution 1:1000) and mouse anti-NeuN antibodies (MAB377, Millipore, dilution 1:1000) in 0.1%NDS/PBS at 4 °C overnight under agitation ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking buffer at the following dilutions and incubated on tissue sections at 4°C overnight: Calb1 (Swant, CB-38a, 1:1000) and Pvalb (Millipore-Sigma, MAB1572, 1:1000). Sections were then washed three times PBS and incubated in anti-mouse or anti-rabbit fluorescently conjugated secondary antibodies (Invitrogen Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with GFAP antibody (mouse anti-GFAP; 3670S; 1:1000; CST, Danvers, MA) and TH antibody (sheep anti-TH; 1:1000; AB1542; Millipore Sigma, Burlington, MA) together at 4°C for 18 hours followed by appropriate secondary antibodies (donkey anti-mouse IgG [H+L] ...
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bioRxiv - Cancer Biology 2023Quote: ... WB 1:1000), anti-ITGA5 (Cat. #: 4705, CST, WB 1:1000) and anti-β-actin (Cat. #: A2228, WB 1:10 000, Sigma-Aldrich Inc.). Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase ...
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bioRxiv - Neuroscience 2023Quote: ... the blot was probed for HA-epitope (rabbit anti-HA, Cell Signaling #3724, 1:1000; or rat anti-HA, Sigma #11867423001, 1:1000), or PSD95 (mouse anti-PSD95 ...
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bioRxiv - Cell Biology 2019Quote: ... The desired Young’s modulus (E) of the pre-mixes was adjusted by mixing pre-defined ratios of 40% (w/v) acrylamide monomer (Sigma-Aldrich, A4058) and 2% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... cells from the ATOH1-EGFP line were gently scraped off Transwell membranes and plated on poly-D-lysine (Millipore, A-003-E) and Laminin (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Pooled culture media containing E/S was concentrated by centrifugation using Amicon ultra centrifugal filter units (MWCO 10 kDa, Sigma-Aldrich, Denmark), and filtered prior to testing of protein content by bicinchoninic (BCA ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal neurons were plated at a density of 120,000 cells per 18-mm-diameter coverslip that was coated with 1 mg/ml poly-D-lysine (Sigma, #A-003-E) and 2.5 μg/ml laminin (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed 2X with YPRaff media (1X with YPRaff and 1X with YPRaff + 50 μg/ml Pronase E (Sigma-Aldrich, 1074330001)) ...
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bioRxiv - Developmental Biology 2023Quote: H&E and tartrate-resistant acid phosphatase (TRAP) staining were performed according to the manufacturer’s protocols (Sigma, St. Louis, MO, United States) as described earlier [25] ...
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bioRxiv - Molecular Biology 2023Quote: ... MV4-11 cells were attached overnight by coating the coverslips in poly-D-lysine (A003-E; Sigma Aldrich; 100µg/ml in H2O) for 1 h ...
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bioRxiv - Genetics 2023Quote: ... The blocks were cut into 4 μm sections and stained with hematoxylin and eosin (H&E) to visualize morphology and with potassium hexacyanoferrate(II) trihydrate (Sigma cat# P3289) to visualize ferric iron deposits and counterstained with Neutral Red to visualize cell morphology (Sigma-Aldrich cat#72210).
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cellular monolayers were then incubated at 37 °C and under 5% CO2 in cell culture medium containing the lysosomal protease inhibitors E-64d (10 μg/mL final concentration; #E8640, Sigma-Aldrich, USA) and pepstatin A (10 μg/mL final concentration ...
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bioRxiv - Microbiology 2024Quote: Supernatants containing VSV(GFP)ΔG particles with S and E were layered on a cushion of 20% sucrose (Sigma-Aldrich, #S9378-1KG) in PBS and centrifuged for 90 min at 4°C and 20,817 x g ...
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bioRxiv - Evolutionary Biology 2021Quote: ... After expression was verified in cell lines by Western blot using anti Flag tag antibodies (Monoclonal ANTI-FLAG® M1, Sigma Aldrich #F3040), surface expression was verified for FPR1 using Thermo Fisher FPR1 polyclonal antibody ref number PA5-33534 ...
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bioRxiv - Biochemistry 2020Quote: ... Multiple clones were isolated and successful integration of N-terminal 3xFLAG tag was validated by western blotting with anti-FLAG-HRP (Sigma, A8592, 1:10,000).