Labshake search
Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Protein loading was controlled by Vinculin (VCL) antibody (1:5000, V3131, Sigma-Aldrich). Horseradish peroxidase conjugated goat anti-mouse IgG antibody (1:10000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein expression was induced using 200µM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma). Bacterial cells were pelleted and resuspended in 20ml Buffer A (for His-tagged ...
-
bioRxiv - Cancer Biology 2019Quote: ... To generate free aldehydes from oxidize sugar groups in matrix protein (fibronectin, Sigma), sodium periodate (∼3.5mg/ml ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein quantification of the cleared lysates was performed by Bradford colorimetric assay (Sigma) using a BSA standard curve ...
-
bioRxiv - Microbiology 2021Quote: ... The following antibodies were used to detect proteins: anti-tubulin (Sigma-Aldrich T6199), anti-IE1 (1B12 ...
-
bioRxiv - Cancer Biology 2022Quote: ... The proteins were separated in SDS-PAGE and transferred to PVDF membrane (Millipore) and subsequently probed with respective antibodies including anti-RAD51 (8875S ...
-
bioRxiv - Cell Biology 2022Quote: ... and anti-mouse GAPDH 0.2 μg/mL (Millipore Sigma, G8795; protein loading control). IRDye®-800CW goat anti-rabbit and −680RD goat anti-mouse IgG secondary antibodies at a 0.1 μg/mL concentration (LI-COR Biosciences ...
-
bioRxiv - Molecular Biology 2022Quote: ... The protein was then concentrated in an Amicon Ultra centrifugal filter (Millipore Sigma) to less than 2 mL total volume ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Cat. #PVH00010 Millipore, Bedford, MA) as described (Popova et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were then transferred to 0.45 µm pore PVDF (catalog number IPVH00010, Millipore) at 25 to 35 V for 1 hour and incubated with appropriate antibodies overnight at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... RNA-YTHDC1-antibody complexes were pulled down using Dynabeads Protein A/G (Millipore) and washed 3 times with NETN100 buffers (20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein alkylation reactions were then carried out with 50 mM Iodoacetamide (IAA) (Sigma) at room temperature for 30 minutes in the dark ...
-
bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was filtered through a 0.22 μm centrifugal filter (Merck Millipore) prior to loading onto the gel filtration column ...
-
bioRxiv - Biochemistry 2022Quote: ... RAF protein was then eluted with 200 μg/mL 3xFLAG peptide (Sigma-Aldrich) in TN buffer ...
-
bioRxiv - Cell Biology 2022Quote: Protein concentration was calculated from either Coomassie Brilliant Blue G (Sigma-Aldrich; B0770) staining of SDS-PAGE-separated purified proteins and Bovine Serum Albumin (BSA ...
-
bioRxiv - Cell Biology 2022Quote: ... 200 ug protein was loaded onto a Microcon Centrifugal Filters (Millipore, Cat: 42407). Filter aided sample preparation (FASP ...
-
bioRxiv - Cell Biology 2022Quote: Total protein was extracted by lysing the cells with RIPA Buffer (Sigma-Aldrich) containing phosphatase and protease inhibitors (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... 350 µg total protein concentrated with 3-kDa cut-off centrifugal filter (Millipore) from serum-free medium was subjected to SDS-PAGE with multiple band excision and in-gel digestion ...
-
bioRxiv - Cell Biology 2022Quote: For analysis of oxidized protein abundance using the Oxiblot assay kit (Millipore, S7150), lysates were prepared in RIPA lysis buffer supplemented with 50 μM DTT ...
-
bioRxiv - Cell Biology 2022Quote: Chemical cross-linking of proteins was performed with dithio-bismaleimidoethane (DTME) (Sigma-Aldrich) dissolved in dimethyl sulfoxide (DMSO) ...
-
bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.2 μm PVDF membrane (#ISEQ85R; Sigma-Aldrich/Merck) by electroblotting the separated proteins for 1 h at 100 V ...
-
bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
-
bioRxiv - Plant Biology 2022Quote: ... Soluble protein was purified with 200 μl of Glutathione agarose beads slurry (Sigma), gently shaking for 1 hour at 4°C ...
-
bioRxiv - Plant Biology 2022Quote: ... proteins were transferred to an Immobilon®-P PVDF membrane (Merck Millipore Ltd.) in a Biometra Fastblot B34 chamber at 1 mA/cm2 membrane for 1.5 h in transfer buffer (20 mM CAPSO pH 11.2 ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein levels were assessed using immunoblots with HA-HRP (Sigma, Catalog number 12013819001) or FLAG-HRP antibodies (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
-
bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
-
bioRxiv - Microbiology 2022Quote: ... Protein fractions were collected from each well and analysed by silver staining (Sigma) and Western blot using monoclonal anti poly histidine alkaline phosphatase antibody (1:20,000 dilution) ...
-
bioRxiv - Microbiology 2022Quote: ... was added to 75 μl of prewashed protein A sepharose beads (Sigma-Aldrich) together with 700 μl lysis buffer (20 mM Tris/HCl pH 8.0 ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF; Millipore) membranes ...
-
bioRxiv - Microbiology 2022Quote: ... Pure Cas protein was finally concentrated in Amicon Ultra centrifugal filter (Merck-Millipore).
-
bioRxiv - Microbiology 2022Quote: ... Digested glycans were cleaned using a 96-well PVDF protein-binding membrane (Millipore) attached to a vacuum manifold ...
-
bioRxiv - Molecular Biology 2022Quote: ... Pellets were transferred to low protein binding tubes (Sigma-Aldrich, Cat# Z666513-100EA) and stored at −80°C
-
bioRxiv - Neuroscience 2022Quote: ... and the separated proteins were then transferred to polyvinylidene difluoride membranes (Millipore, USA). These membranes were blocked in 5% (w/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany). Unspecific binding on the PVDF membrane was blocked by incubation with the blocking buffer (5% non-fat milk ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were resuspended in 50 mM triethylammonium bicarbonate (TEAB, Sigma cat. no. T7408), reduced for 1 hour at 55°C using 10 mM tris(2-carboxyethyl)phosphine (TCEP ...
-
bioRxiv - Neuroscience 2023Quote: ... Residual protein fragments were precipitated with trichloroacetic acid (Sigma-Aldrich, cat. no. T6399). The supernatant ...
-
bioRxiv - Microbiology 2022Quote: ... YifLStrep protein was eluted with wash buffer containing 2.5 mM d-Desthiobiotin (Sigma).
-
bioRxiv - Neuroscience 2022Quote: ... and mouse anti-glial fibrillary acidic protein (GFAP; 1:500, #G3893; Sigma-Aldrich). The next day sections were washed 3 times with the blocking buffer (15 min each ...
-
bioRxiv - Neuroscience 2022Quote: ... The expression of neuronal marker protein NeuN (anti-NeuN, 1:200, MAB377, Millipore) was determined to identify neuronal tdTomato expression.
-
bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma-Aldrich, USA). Protein lysates were prepared in a mixture of RIPA and Lamelli Buffer to produce a final protein concentration of 5 ug/ul ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resolved protein samples were transferred onto Immobilon-P PVDF Membrane (Millipore Sigma), and the blots incubated in the presence of primary antibodies (provided in table S1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Equal loading of protein samples was confirmed via Ponceau S staining (Sigma-Aldrich) for 4 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge at 4 °C ...
-
McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... Eluted protein fractions were pooled and concentrated using Amicon 30K Centrifugal filters (Millipore). The concentrated ...
-
bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing protein were concentrated using Amicon Ultracel-10K/ Ultracel-30K (Millipore) centrifuge filters and subsequently applied to size exclusion chromatography (SEC ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 μg protein was combined with 1 μL 10% Triton X-100 (Sigma) and brought to a volume of 33 μL in Vance buffer (225 mM D-mannitol ...