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Citations for Millipore Sigma :
5401 - 5450 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-heat shock protein 70 (HSP70) (H5147, Sigma-Aldrich, 1:500), rabbit monoclonal anti-Alix (MCA2493 ...
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bioRxiv - Microbiology 2022Quote: Coding sequences for M proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site (5) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
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bioRxiv - Neuroscience 2022Quote: Total protein from A1 was extracted using RIPA buffer (#R0278, Sigma Aldrich, USA) and protease inhibitor cocktail kit (#78410 ...
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bioRxiv - Cancer Biology 2022Quote: ... required protein amounts resolved by SDS-PAGE and transferred to nitrocellulose membrane (Millipore). Western blot analysis was performed according to standard procedures ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were incubated with 3ul anti-FLAG antibody (Clone M2, Sigma-Aldrich) and 50ul Protein-G magnetic beads (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Misfolded proteins were detected by means of thioflavin-S (Sigma Aldrich; 1:1000) staining ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and proteins were transferred to a polyvinylidene difluoride (PVDF; Millipore, Bedford, MA, USA). Membranes were blocked with bovine serum albumin (1 % ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were digested by addition of 20 μL of proteinase K (Sigma, P6556) at 20 mg/ml to the samples and incubation for 2 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using 10kDa Amicon filter (Merck Millipore, Burlington, MA, USA). The protein concentration in each fraction was estimated using the Bradford reagent (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched with a Roche EDTA protein inhibitor tablet (Sigma-Aldrich) at 1X concentration and the solution was then passed over a CaptureSelect™ IgG-CH1 column (ThermoFischer Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred onto a 0.45 μm methanol-activated PVDF membrane (Immobilon, Millipore) overnight at 4°C at 25V and 45 min at 4°C at 75V the next day ...
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bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... and MBP-tagged and associated proteins were eluted in 60 mM maltose (Sigma) for 30 minutes on ice.
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bioRxiv - Cancer Biology 2022Quote: ... filtered through a low protein binding filter unit (PVDF, 0.45 um, Sigma-Aldrich), and stored at 4 degrees C for up to 5 days prior to use ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... Proteins and phosphorylation-sites were transferred to a polyvinylidene difluoride membrane (PVDF, Millipore), where the PVDF membrane was blocked after the transfer (5 min ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were then concentrated using AmiconTM Ultra 15 concentrators (MERCK Millipore) and stored in -80°C.
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 100 µg protein where loaded on a gel to detect endogenous MDGA proteins in Western blots ...
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bioRxiv - Plant Biology 2021Quote: ... 8 μg of purified proteins were incubated with 1 mM DMAPP (Sigma-Aldrich) and 1 mM AMP or ATP for two hours at 30°C in 12.5 mM Tris–HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... The protein bands were detected using 3,3 ́-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma, D5637). The blots were incubated in a solution containing 1mg/ml DAB in 50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). The membranes were incubated with appropriate mouse antibodies directed against TLR4 (ab22048 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and protein concentration was measured by Bradford assay (Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... proteins were precipitated twice by addition of 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Neuroscience 2020Quote: ... and glial fibrillary acidic protein (GFAP) conjugated Cy3 produced in mouse (Sigma, C9205). Slides were cover-slipped with ProLong Gold with DAPI (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were quantified through a Bradford assay kit (Sigma -Aldrich, USA). The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... protein was then loaded on to Fractogel EMD SO3- cation exchange column (Millipore) in 20 mM MES ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were detected via western blotting with M2 anti-FLAG (Sigma-Aldrich, F1804) and THE anti-Strep II tag FITC (Genescript ...
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bioRxiv - Neuroscience 2021Quote: ... To elute proteins from the beads a 3xFLAG peptide (100 ug/mL, Sigma) with 0.05% RapiGest in IP buffer was added to the beads for 15 minutes at room temperature with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using a 30 kDa cutoff centrifugal filter unit (Millipore) and chromatographed on a Superdex 200 increase column in a final buffer of 20 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in Myc wash buffer). The eluted FLAG-ACBD5 was incubated with the Myc-VAPB-bound beads for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized using HRP-conjugated secondary antibody and chemiluminescent HRP substrate (Millipore).
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bioRxiv - Cell Biology 2021Quote: Wash 20μl Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) twice with 1 ml FA buffer (10mM HEPES[PH7.5],150mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were incubated with primary antibodies against Foxp4 (Millipore, #ABE74, 1:1000), Ucp1 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The purified proteins were further concentrated on Amicon Ultra Centrifugal Filters (Sigma-Aldrich) and quantified using Qubit protein assay.
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was concentrated using a 10 kDa MWCO filter (Amicon-Millipore), and concentrated up to 2 mg/mL.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then transferred to an Immobilon-FL PVDF membrane (Millipore, Billerica, MA) on ice either for 2 h at 100 V ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were incubated with antibody and protein A/G magnetic beads (Sigma) from 3 h to overnight at 4°C under rotation conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... 60 μL of Magna ChIP™ Protein A+G Magnetic Beads (Merck Millipore) was used in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... the separated proteins were transferred onto 0.45 μm polyvinylidene difluoride membranes (Millipore, USA). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... denatured proteins were loaded onto 30 kDa amicon filter (Merck Millipore, Darmstadt, Germany). The buffer was exchanged with UA solution (8 M UREA in 0.1 M TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole was removed and the protein was concentrated using a Centricon (Merck Millipore) and preserved at −80 °C in small fractions at 2 mM concentration.
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Biophysics 2020Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37 °C in LB medium containing 50 μg ml−1 kanamycin and 34 μg ml−1 chloramphenicol till an OD600 nm of 0.7 was reached ...
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bioRxiv - Cancer Biology 2021Quote: ... Co-Immunoprecipitation was performed using PureProteome™ Protein A Magnetic Beads (Millipore, LSKMAGA10). Protein extracts were first precleared using 10μl of beads for 200μg of proteins (1-hour incubation at 4°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein was also visualized using 4-Chloro-1-naphthol solution (Sigma Aldrich) which is a substrate for HRP in a reaction that results in a colored precipitate.
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bioRxiv - Plant Biology 2021Quote: ... GST-PPKs were tested against 5 µg myelin basic protein (MBP; Sigma Aldrich) and 5 µg Casein in 0.1 M Tris pH 8.8 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rat anti-mCherry (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... resolved proteins were further transferred to a PVDF membrane (0.45 μm; Millipore, USA) for immunoblotting and the blots were blocked using Starting Block T20 (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using a 50-kDa cutoff Amicon filter (Sigma-Aldrich) and purified on a Superose 6 Increase 10/30 gel filtration column (GE Healthcare) ...