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Citations for Millipore Sigma :
5501 - 5550 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using a standard Bradford assay reagent (B6916, Sigma Aldrich).40µg total protein were loaded per samples on a pre-cast gradient 4-12% gel (NW04120 ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were concentrated using Amicon Ultracel-30 Centrifugal Filter Units (Merck Millipore) for wild-type RF2 and RF2-GGP and Ultracel-3 for ResQ62-His6 hereafter referred to as ResQ.
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Immunology 2019Quote: ... 6 μl (=10 μg) total protein was then reacted with streptavidin (Sigma, S4762) in PBS (total reaction volume 24 μl ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were separated on 5.5% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting for 50 min ...
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bioRxiv - Genetics 2020Quote: Proteins were reduced with adding 1.5 μl of 200 mM dithiothreitol (DTT, Sigma) in 500 mM AmBic and incubated at 37 °C for 1 h with shaking at 400 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... Myc-tagged proteins were immunoprecipitated with 4AG Myc monoclonal agarose beads (EMD Millipore), washed three times in lysis buffer and boiled in 2X Laemmli reducing buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a Immobilion-P PVDF membrane (Millipore Sigma, MA, USA) at 47V overnight at 4°C using Mini Trans-Blot® Cell (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50 kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at −80 °C.
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bioRxiv - Molecular Biology 2020Quote: ... fractions containing protein were concentrated in 10 kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at −80 °C.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were detected by the ECL method (Immobilon Western Chemiluminescent HRP Substrate, Millipore), and loading control of the western blot membrane was performed using the Revert total protein stain (LI-COR Bioscience).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). The following antibodies were used for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then transferred onto a polyvinylidene difluoride membrane (Millipore, Toronto, Canada) and probed using antibodies for PI4KIIIβ (BD Biosciences 611817 ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were buffer exchanged into 200 mM ammonium acetate buffer pH 7.4 (Sigma), supplemented with 0.02% DDM for ACKR1 and for toxins ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was then loaded onto a 20 ml chelating Nickel HiTrap column (Sigma) that had been equilibrated in Ni column binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were buffer exchanged into 200 mM ammonium acetate buffer pH 7.4 (Sigma) using Bio-Spin microcentrifuge columns (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred to methanol-activated Immobilon-P PVDF membrane (Millipore IPVH00010) for 90 minutes at 25V at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 750µg total protein was then incubated with 20µg prewashed FLAG affinity gel (Sigma) for 2h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and for utrophin samples were quantified against total protein using Coomassie stain (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... All proteins were expressed in Escherichia coli BL21(DE3) Rosetta/pLysS strain (Novagen). Cells were grown in LB medium supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore), concentrated using VIVAFLOW 50R (Sartorius ...
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bioRxiv - Microbiology 2020Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore) and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... antimicrotubule-associated protein 2 (MAP2) Alexa Fluor 647 (1:500, NB120-11267AF647; Millipore). Cell nuclei were stained by using Hoescht 33342 (2 μg/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... The eluted proteins were concentrated using Amicon® Ultra Centrifugal Filters (Millipore Sigma). The concentrated proteins were dialyzed into SUMO-SIM protein buffer (150 mM KCl ...
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bioRxiv - Biophysics 2020Quote: ... Purified labelled protein was concentrated using a 50kDa MWCO centrifugal filter (EMD Millipore) and stored at −80°C in buffer containing 10mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... The resulting protein samples were individually incubated with anti-GFP agarose beads (Sigma) for 4 h at 4°C followed by five rinses in an ice-cold PBS buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were reduced and alkylated for 1 h with 15 mM TCEP (Sigma) and 30 mM CAA (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: The immunoprecipitated protein eluates were treated with 0.1 U of benzonase (Sigma, E8263) for 30 min at 37 °C and further diluted with the same volume of 100 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins blotted onto nitrocelullose membranes were probed with anti-HA H3663 (Sigma-Aldrich), MyD88 4D6 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: All proteins were expressed and purified from E.coli Rosetta (DE3) Competent Cells (Novagen). Details of individual constructs and their expression plasmids are detailed in the plasmids section ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were then alkylated by the addition of 10 mM iodoacetamide (Sigma-Aldrich) for 45 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated using an Amicon Ultra 10 K device (Merck Millipore), loaded on a size exclusion column [ENrich SEC 650 10 x 300 Column (Bio-Rad)] ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced by the addition of tetracycline (1 μg/ml; Sigma) for desired time (determined during time course).
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bioRxiv - Biophysics 2021Quote: ... The protein eluent was concentrated by a 100-kDa cut-off Centricon (Millipore) and further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then eluted with 5 folds volume of Glutathione solution (Sigma-Aldrich), and were condensed using 10kD protein concentrators (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge and flash frozen in liquid nitrogen.
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bioRxiv - Immunology 2020Quote: ... Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). For the endogenous interaction assay ...
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bioRxiv - Microbiology 2020Quote: ... proteins were fractionated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore), and probed with the indicated antibodies.
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bioRxiv - Microbiology 2020Quote: ... cut protein bands were first DTT reduced (Sigma-Aldrich, St. Louis, MO, USA) to be subsequently treated with Iodacetamide for protein alkylation (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... washed in PBS and processed using ProteoExtract Native Membrane Protein Extraction Kit (Sigma). Soluble cytoplasmic ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysates were incubated with either anti c-MYC agarose beads (Sigma A7470), anti c-MYC magnetic beads (Santa Cruz 500772) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with anti-FLAG M2-HRP (1:3000 dilution, Sigma #A8592).
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bioRxiv - Immunology 2020Quote: ... followed by binding of protein A/G magnetic beads (Merck Millipore cat. LSKMAGAG02) for 4 h at 4℃ ...
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bioRxiv - Microbiology 2021Quote: ... The protein bands were visualised using a ProteoSilver Silver Stain kit (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... Protein loading was determined using an anti-β-actin (AC-74, Sigma-Aldrich) antibody ...
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bioRxiv - Cell Biology 2020Quote: ... 1mg protein lysates were incubated with 30ul Flag antibody-conjugated agarose beads (Sigma) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were detected by enhanced chemiluminescence (Classico substrate, Immobilon-Millipore, USA, #WBCUC0500) and visualized by G ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane (Amersham™ Protran®, Sigma-Aldrich). For blot development and detection ...
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bioRxiv - Genomics 2021Quote: ... and rabbit anti-Protein A antibodies (Sigma, Cat No. P3775, dilution 1:1000) were added ...