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Citations for Millipore Sigma :
5551 - 5600 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Media was then swapped to 200 ng ml-1 FGF9 (R&D, 273-F9-025) and 1 µg ml-1 heparin (Sigma Aldrich, H4784) in TeSR-E6 for 5 days to induce nephron progenitors ...
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bioRxiv - Microbiology 2023Quote: ... Spectinomycin resistant transformants were transformed with a plasmid carrying a FLP recombinase which was induced by 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) (Fischer BioReagents, 367-93-1) and 1.5 mM theophylline (Sigma, T1633-100G), allowing for the removal of the spectinomycin resistance cassette via recombination ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated cultures transduced with BirAER either by electroporation or infection were maintained in medium supplemented with 10 µM D-biotin (Sigma- Aldrich, #B4639).
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bioRxiv - Cell Biology 2024Quote: ... parasites were treated with 5% D-sorbitol treatment and then split into ±aTC conditions and further separated in ±700 nM DHA (Sigma-Aldrich D7439) treatment ...
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bioRxiv - Immunology 2024Quote: ... These diluted test peptides were co-incubated with 0.1 μM biotin-labeled peptide and 50 nM purified MHC-II proteins (ProImmune, Oxford, United Kingdom) at 37°C for 24h in the presence of Octyl-β-D-glucopyranaside (Sigma-Aldrich). Then ...
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bioRxiv - Immunology 2024Quote: ... The lungs were harvested and grossly homogenized with scissors in HBSS containing 2 mg of collagenase D (Sigma Aldrich cat. no. 11088866001) and 0.25 mg of DNase I (Sigma Aldrich cat ...
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bioRxiv - Neuroscience 2024Quote: Tgg were dissected from E13.5-E15.5 WT and Nogo-A KO embryos and cultured on glass coverslips coated with 20 µg/mL poly-D-lysine (Sigma-Aldrich, #P0296) and 2 µg/mL Laminin (Sigma-Aldrich ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... cauda epididymis and vasa deferentia from FVB or PWD males were dissected and sperm was gently squeezed out into capacitation medium (HTF supplemented with MBCS at final concentration of 0.75 mM; HTF: Sigma-Aldrich #MR-070-D, MBCD: Sigma-Aldrich #C4555) and allowed to swim up for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-cell stage embryos were collected and transferred to KSOM embryo culture medium (Embryo Max KSOM, Millipore-Sigma, catalog # MR-121-D), and further cultured for additional 2-3 days to hatching/hatched blastocysts ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-cell stage embryos were collected and transferred to KSOM embryo culture medium (Embryo Max KSOM, Millipore-Sigma, catalog # MR-121-D), and further cultured for additional 2-3 days to hatching/hatched blastocysts ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-cell stage embryos were collected and transferred to KSOM embryo culture medium (Embryo Max KSOM, Millipore-Sigma, catalog # MR-121-D) and further cultured for an additional 2-3 days to hatching/hatched blastocysts ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-cell stage embryos were collected and transferred to KSOM embryo culture medium (Embryo Max KSOM, Millipore-Sigma, catalog # MR-121-D) and further cultured for an additional 2-3 days to hatching/hatched blastocysts ...
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bioRxiv - Biophysics 2024Quote: ... was glycated in acidic conditions (20 mM acetic acid at pH = 3.2) at a concentration of 2 mg/mL with varying concentrations of D-ribose (Sigma, 50-69-1). All samples were incubated at room temperature for varying lengths of time from 7 - 350 days and then ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then incubated with primary antibodies (1:500 goat anti human/mouse TrkB, R&D Systems #AF1494; 1:1000 rabbit anti-mouse MAP2, Millipore #AB5622 ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then incubated with primary antibodies (1:500 goat anti human/mouse TrkB, R&D Systems #AF1494; 1:1000 rabbit anti-mouse MAP2, Millipore #AB5622; 1:200 mouse anti-mouse PSD95, Sigma #P246 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were subcultured on glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... The β- hexosaminidase assay was performed in a 96-well plate by mixing 50 μL of 2 mg/mL 4-nitrophenyl N-acetyl-β-D-galactosaminide (N9003, Millipore Sigma) in 0.1 M citrate buffer (pH 4.5 ...
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bioRxiv - Neuroscience 2024Quote: ... All culture dishes and plates were pre-coated at ambient temperature for at least 30 min with sterile-filtered 100 µg/mL poly-D-lysine (Millipore-Sigma or ThermoFisher) dissolved in cell-culture grade water (Corning ...
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bioRxiv - Microbiology 2024Quote: Induction of the isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter (Plac) was conducted by supplementing the medium with IPTG (Sigma-Aldrich) at the required concentration (40μM – 500 μM) ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... the induced cells were passaged as single cells with Accutase and seeded at 2×106 cells per Poly-D-Lysine coated 6 wells (Sigma Cat #P6407) in Cortical Neuron Culture Medium 1 (CM!) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were next embedded overnight at RT in Durcupan resin (20g component A, 20g component B, 0.6g component C, 0.4g component D; Sigma Canada, Toronto, cat# 44610). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... the same mice were injected with D-amphetamine (1.5 mg/kg dissolved in sterile 0.9% saline, IP; Millipore-Sigma A5880, Burlington, MA), 20 minutes before interval timing sessions and recordings were performed.
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bioRxiv - Molecular Biology 2024Quote: ... 293T cells expressing these proteins were seeded 24 hours prior to infection at 3x104 cells/well in the 48-well plates coated with 0.1 mg/mL poly-D-lysine (Sigma-Aldrich, Cat# P6403). The same cells were also plated on the 6-well plates to assess TIM1 expression level ...
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bioRxiv - Physiology 2024Quote: ... Animals were fasted during 6h starting at 8 AM and then gavaged with 2 mg/g of D-(+)-glucose (Sigma Aldrich, G70021). Glycemia was measured from the tail vein using a Verio Reflect glucometer (OneTouch ...
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bioRxiv - Biochemistry 2024Quote: ... protein solutions were added into isotonic solutions characterized by varied ratios of KCl to N-methyl-D-glucamine (NMDG, Sigma-Aldrich, USA), all prepared in Tris/MES buffers (pH 7.4) ...
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bioRxiv - Genomics 2024Quote: The genomic DNA band from the CHEF gel was excised and placed inside a D-Tube Dialyzer (Millipore, CAT#71508-3, USA). The D-Tube was filled with a 0.5×TBE buffer and placed in the middle of the chamber of the Mapper XA system subjected to switching intervals of 1,200-3,600 seconds for 24 hours ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed with PBS and incubated with 100 µM 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2NBDG) (Sigma Cat# 186689-07-6) containing RPMI1640 (HiMedia Cat# AT150 ...
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bioRxiv - Cell Biology 2024Quote: ... were assayed in a final volume of 50 µl with 5 mM 4-methylumbelliferyl(MU)-N-acetyl-β-d-glucosaminide (Sigma-Aldrich), 5 mM 4-MU-α-d-galactopyranoside and 5 mM 4-MU-β-d-galactopyranoside (Calbiochem ...
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bioRxiv - Cancer Biology 2024Quote: ... Ptbp2 O/E LAMA84 cells were maintained in RPMI containing 10%FBS in 5% CO2 incubator at 37 □ Cells were treated with Actinomycin D (Sigma, Cat#A4262) @5ug/ml for different time points ...
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bioRxiv - Cancer Biology 2024Quote: ... Buffer on top of cells was collected and further incubated with 3 mM 4-nitrophenyl-N-acetyl-β-D- glucosaminide substrate (Sigma, N9376) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 2x 10 mL PBS and replaced with 20 mL glucose-free DMEM + 10% dialyzed FBS + 50 µM D-biotin (Sigma-Aldrich B4639). 16 mM D-glucose was added to control plates ...
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bioRxiv - Cancer Biology 2024Quote: CytoView 96-well impedance plates (Axion Biosystems, Atlanta, GA) were prepared by coating with 0.1 mg/mL poly-D-lysine (Sigma Aldrich, Burlington, MA) then 20 µg/mL laminin (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Iowa City, IA), dynein heavy chain (P1H4, gift from T. Hays, University of Minnesota, Minneapolis, MN) and anti-HA antibody (Sigma, H 6533 (clone HA-7).
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bioRxiv - Immunology 2021Quote: ... At 0 min and 1 h the sample was analyzed for concentrations of inorganic monophosphates with the ATPase/GTPase Activity Kit (Sigma-Aldrich, Cat. no: MAK113-1KT) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and stored in 10% formalin for 48 h at 4°C then dehydrated in 30% sucrose in 1X PBS (Sigma, St Louis, MO, cat# S7903) for 48 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated with BCNU (125 μM) for 24 h before treating with cytochalasin-B (2 μg/mL) (Sigma Aldrich; Cat# 250233, St. Louis, MO) for an additional 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... for 30 min at RT followed by 1 h incubation at RT with rabbit anti-FLAG antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA) and mouse anti-SEC31A antibody (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Induced spores were harvested at 8 h after the dilution and were purified from the remaining vegetative cells with Histodenz (Sigma-Aldrich, St. Louis, Missouri, USA) density gradient centrifugation (18) ...
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bioRxiv - Microbiology 2021Quote: ... and harvested after 72 h by centrifugation followed by resuspension in ice-cold PBS containing 1 mM MgCl2 and CaCl2 (Sigma-Aldrich, St. Louis, MO, USA). Bacterial suspensions were homogenized by 50 passages through a syringe with a 25G needle and measured for optical density ...
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bioRxiv - Neuroscience 2022Quote: ... we used donkey anti-goat Igg H&L (Alexa Fluor® 647) Preadsorbed (Ab150135, 1:50) and sheep anti-rabbit Cy3 (Sigma–Aldrich C2306, 1:100).
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bioRxiv - Neuroscience 2022Quote: ... 4 well chamber slides (177437, Thermo Fischer Scientific) were coated by incubating for 12 h with filter sterilized poly-L-lysine (P8920, Sigma-Aldrich; 100 μ g/ml) in borate buffer (boic acid 3.1g/l ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Plant Biology 2022Quote: ... and K+- and H+-selective microelectrodes were front-filled with commercially available ionophore cocktails (Product numbers 60031 and 95297, respectively; Sigma-Aldrich, St. Louis, MO, USA). Three microelectrodes were mounted on a 3D-micromanipulator (MMT-5 ...
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bioRxiv - Molecular Biology 2024Quote: Peripancreatic fat samples were embedded at the Louise Pelletier Histology Core Facility and tissue sections were stained with H&E (Epredia™ Modified Harris Hematoxylin, Fisher Scientific, Cat # 22-050-206; Eosin Y disodium salt, Sigma-Aldrich, Cat # E4382) in the Bruin Lab ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.4) for 1 h at room temperature and were subsequently incubated with primary antibodies rabbit anti-claudin-5 (Sigma-Aldrich, cat# SAB4502981, AB_10753223, 1:300), mouse anti-claudin-5 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then pulsed for 1 h with 1 mg/mL FITC-Dextran (10k MW)/ TMR-Dextran (10k MW) (Sigma-Aldrich, St Louis, MA, USA) diluted in OPTI-MEM and then washed with PBS followed by addition of growth media ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 ng of F(ab’)2-Rabbit anti-Rabbit IgG (H+L) (CST, 4412S, Lane Danvers, MA, USA) was diluted in 0.1% Triton X-100 (Sigma-Aldrich, T8787, St. Louis, MO, USA) and incubated at 95°C for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% [v/v] Triton X–100) and incubated at 37°C for 3 h either with α–mouse plus and α–rabbit minus for PLA assay (Sigma–Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Microbiology 2023Quote: ... yet cells were incubated for 1 h with 150 µL/well LCMV NP (in-house, 1:1000 in blocking buffer) and FLAG (Sigma, 1:500 in blocking buffer) primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... The top part of the membrane was blocked with 3% non-fat milk in Tris-buffered saline with 0.1% Tween (TBST) followed by incubation at room temperature (RT) for 1 h with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich, USA; #F3165, 1:2,000) to detect the overexpressed EPHB1 receptors proteins (130 KDa) ...