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Citations for Millipore Sigma :
5501 - 5550 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal neurons were plated at a density of 120,000 cells per 18-mm-diameter coverslip that was coated with 1 mg/ml poly-D-lysine (Sigma, #A-003-E) and 2.5 μg/ml laminin (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μl of dilution enzyme solution (0.5 μM) was combined with 100 mg/ml N-acetyl-D-glucosamine (Sigma Aldrich, A3286-5G). Cells were allowed to sit on ice for 5 minutes to allow WGA to bind fully ...
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 μM cytochalasin D (CytD; Enzo, Lausen, Switzerland; from 5 mM stock in DMSO: dimethyl sulfoxide; Sigma-Aldrich, St. Louis, Missouri) before using AM4-64 ...
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bioRxiv - Cell Biology 2020Quote: ... Both naïve ESCs and D-EPSCs were cultured at 20% O2 and 5% CO2 at 37°C on mitomycin C (MMC; Sigma-Aldrich M0503) inactivated mouse embryonic fibroblast (MEF ...
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bioRxiv - Immunology 2020Quote: ... The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G) in PBS ...
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bioRxiv - Neuroscience 2021Quote: Cdh11 wild-type and knockout hippocampal neurons were seeded in triplicates at a density of 30,000 cells per well on a 96-well plate coated with 20 μg/ml poly-D-lysine (Millipore Sigma Cat#P6407). At 7 DIV neurons were infected with the IncuCyte NeuroBurst Orange lentivirus under a synapsin promoter (Essen Bioscience Sartorius Cat# 4736) ...
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bioRxiv - Neuroscience 2022Quote: ... glia-containing samples were cultured with NBD supplemented with 2 μM of cytosine-β-D-arabinofuranoside (AraC, 500 μM in ultrapure water, C1768, Sigma Aldrich) to inhibit astrocyte and microglia proliferation ...
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bioRxiv - Bioengineering 2022Quote: ... The reaction was started by adding 30 µL of 100 mM (final concentration 5 mM) substrate 4-nitrophenyl β-D-glucopyranoside (Sigma-Aldrich). At regular five-minute intervals ...
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bioRxiv - Neuroscience 2022Quote: ... Figure 3 – figure supplement 1C&D) or 0 mM CaCl2 together with 2 mM of the Ca2+ chelator EGTA-tetrasodium salt (Sigma, Germany, E8145) to buffer residual Ca2+ traces (Figure 3 – figure supplement 1A&B) ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Biochemistry 2022Quote: ... Then the gene expression was induced by the addition of 1 mM (final concentration) of isopropyl-β-D-1-thiogalactopyranoside (IPTG, Sigma-Aldrich). After 3 h of induction at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed with D-PBS-/- and incubated for 5 min at 37řC with 1 ml of accutase solution (Sigma-Aldrich A6964-500ML). The plate was gently tapped to detach cells and cells were gently pipetted to generate a single cell suspension ...
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G; 2,46 g/L NaHCO3, Sigma Aldrich, S5761-500G ...
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bioRxiv - Physiology 2022Quote: ... a sharp glass pipette (tip inner diameter: 15–30 μm) filled with NMDA (N-methyl-D-aspartate, Sigma-Aldrich, M3262, 0.2 mM), C21 (200 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 times with D-PBSX before incubation with the corresponding secondary antibodies (all diluted 1:500) and DAPI (Sigma, #32670-25mg) for 1 h (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Physiology 2022Quote: ... Protein contents of mitochondrial pellets were extracted by adding 4X volume of 1x MAS buffer followed by 1/9 volume of 10% n-dodecyl β-D-maltoside (DDM; Sigma D4641) for a final 1% DDM concentration ...
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bioRxiv - Molecular Biology 2022Quote: Gus activity was determined to quantify chitin binding of Gus-Cts1 using the specific substrate 4-methylumbelliferyl β-D galactopyranoside (MUG, Sigma–Aldrich). To his end 50 μg of U ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were cultured for a period of 48 hours on 35-mm-diameter culture dishes coated with 0.1 mg/ml poly-D-lysine (Sigma Aldrich, P0899-50MG). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... and seeded between 0.5×105 – 1×105 per well in 96 well plate coated with 10 µg/mL Poly-D-lysine hydrobromide (Sigma – Cat. P7886). The medium culture was changed three hours after the seeding and every two days until the seventh day when the cells developed a typical morphology of a mixed neuron culture.
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bioRxiv - Molecular Biology 2024Quote: ... Exponentially proliferating cells were grown to confluence and then left at confluence for 48 hours before incubation in differentiation media (DMEM supplemented with 10% FBS, 4 mM L-Glutamine, 100 U/ml penicillin/streptomycin, 1 μM dexamethasone (Sigma, D-2915), 1 μg/ml insulin (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Developmental Biology 2024Quote: ... cauda epididymis and vasa deferentia from FVB or PWD males were dissected and sperm was gently squeezed out into capacitation media (HTF supplemented with MBCS at final concentration of 0.75 mM) (HTF: Sigma-Aldrich #MR-070-D; MBCD: Sigma-Aldrich #C4555), and allowed to swim up for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The bound mPTER proteins were eluted by 2.5 mM D-Desthiobiotin before passing through a HiPrep 16/60 Sephacryl S-200 size-exclusion column (Sigma, GE17-1166-01) in buffer containing 25 mM Tris and 100 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ACE2-HEK293 ΔRIG-I cells were seeded at 5×10E4 cells per well in 24-well plates that had been treated with Poly-D-lysine hydrobromide (Sigma, P6407-5MG) and reverse transfected with 50 nM/well of control (CTRL ...
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bioRxiv - Neuroscience 2023Quote: ... glia were plated on micro-islands of growth permissive substrate (mix of collagen I and poly-D-lysine, Corning 354236 and Sigma P6407, respectively) that were printed on a layer of 0.15% agarose.
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bioRxiv - Neuroscience 2023Quote: ... OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα) (1:4000) (Supplementary Table 2) and then seeded on coverslips coated with poly-D-lysine (20 μg/ml) (Sigma-Aldrich; P0899) and incubated at 37°C and 10% CO2 in Sato medium ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos are recovered at E1.5 or E2.5 by flushing oviducts or/and uteri from plugged females with 37°C FHM (LifeGlobal, ZEHP-050 or Millipore, MR-122-D) using a modified syringe (Acufirm ...
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bioRxiv - Cancer Biology 2023Quote: ... Embryos at the blastocyst stage were collected at approximately 72 hours after miRNA microinjection (~E3.5) and placed in M2 (Millipore, MR-015-D) and stored in −80°C.
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bioRxiv - Microbiology 2023Quote: ... These intestinal pieces were washed thoroughly with PBS and digested for 30 min at 37 °C with 0.5 mg/mL collagenase D (Merck/Sigma-Aldrich, cat#11088858001) in RPMI medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were intravitreally injected with 1.5 μl of 20 mM N-methyl-d-aspartic acid (NMDA) (Sigma-Aldrich, St. Louis, MO). The 1.0mg/mL commercially available morphine solution (M-005-1ML in methanol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were seeded in a T75 flask pre-coated with 100 μg/ml of Poly-D-Lysine hydrobromide (PDL; P6407, Sigma, MO, USA) to enhance the primary cell attachment ...
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bioRxiv - Cancer Biology 2023Quote: ... and transcardially perfused with 10 mL PBS containing 1.5 mg D-luciferin potassium salt and 10 mg/L heparin sodium salt (Sigma Aldrich, Cat: H3393). Whole brains were immediately isolated ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 × 104 cells were seeded per well in 24-well MatTek glass-bottom plates (P24G-1.5-10-F) coated with 0.1 mg/ml poly-D-lysine (Sigma; 30,000-70,000 mol wt). Cultures were maintained in organoid base medium without small molecules ...
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bioRxiv - Developmental Biology 2023Quote: ... Pharmacological induction of gut barrier defects was achieved by rearing third instar larvae on food containing 100 µM polyoxin D (Sigma Aldrich # 529313) for 48 hr ...
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bioRxiv - Cell Biology 2023Quote: ... followed by growing them in LB media at 37°C and inducing them by 1 mM isopropyl β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) overnight at 25°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were starved for 24 h in DMEM without FBS and cell proliferation was inhibited by treating the cells with 10 μM Cytosine β-D-Arabinofuranoside (AraC, C1768, Sigma-Aldrich) for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... MV4-11 cells were attached overnight by coating the coverslips in poly-D-lysine (A003-E; Sigma Aldrich; 100µg/ml in H2O) for 1 h ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... at room temperature for 30 minutes in a 96-well flat-bottom plate pre-coated with poly-D-lysine (Sigma-Aldrich #P7280). An Axio Observer 7 microscope was used to image 10% of each well immediately after staining ...
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bioRxiv - Biophysics 2023Quote: ... Poly-D-lysine coating was done by incubating the wells for 20 minutes at 37°C in a 0.4 mg/ml solution (4X) of poly-D-lysine (Merck, P7280-5MG, Sigma-Aldrich, Darmstadt, Germany), made from a 1 mg/ml stock ...
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bioRxiv - Biochemistry 2023Quote: ... dried phosphopeptides from each fraction were re-dissolved in 800 μL cold IAP buffer (50 mM Tris-HCl, 150 mM NaCl, 1% w/v n-Octyl-β-D-glucopyranoside (EMD Millipore), EDTA-free Halt protease inhibitor and phosphatase inhibitor cocktails ...
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bioRxiv - Biochemistry 2023Quote: ... higher temperature and/or purification of the reaction mix did not yield a more homogenous sample as several screening datasets were collected and analysed.) Incubation with n-Octyl-β-D-glucopyranoside (Sigma Aldrich) at a final concentration of 0.1 % (w/v ...
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bioRxiv - Cell Biology 2023Quote: ARPE19 cells were plated at 40,000 cells per well on a Seahorse XF platform compatible 96-well plate pre-coated with poly-D-lysine (Sigma Aldrich, USA) and allowed to adhere and grow for 24 h28 ...
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bioRxiv - Immunology 2023Quote: ... The lungs were harvested and grossly homogenized with scissors in HBSS containing 2 mg of collagenase D (Sigma Aldrich cat. no. 11088866001) and 0.25 mg of DNAse I (Sigma Aldrich cat ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were seeded at a density of 80,000-100,000 cells per well on glass coverslips (Bar Naor, Cat#BN1001-12-1-CN) coated with poly-D-Lysine (Sigma, Cat#P0899). Cells were plated in Neurobasal-A medium supplemented with 2% B27 (Thermo-Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were embedded in Durcupan resin (20 g component A, 20 g component B, 0.6 g component C, 0.4 g component D; Sigma Canada, Toronto, cat# 44610) overnight ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal neurons were sparsely cultured from post-natal day 0-1 pups on glass coverslips coated with poly-d-lysin (Sigma-Aldrich, P7886). When neurons reached 14 days in-vitro (DIV-14) ...
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bioRxiv - Cell Biology 2023Quote: ... Perturbation of cytoskeleton during in vitro maturation was achieved by overnight exposure of morphologically normal GV oocytes to drugs known to interfere with the function of actin network (1 µM cytochalasin D, C8273, Sigma Aldrich; 5 µM brefeldin A, B7651, Sigma Aldrich) and microtubules (1 µM paclitaxel ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The culture was diluted 1:100 and SEP expression was induced at the exponential growth phase by the addition of 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma–Aldrich) for 4h at 30°C under constant stirring ...