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Citations for Millipore Sigma :
5451 - 5500 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The relative msfGFP measurements were normalised for their respective OD600 values and subsequently converted to absolute units of the calibrant 5(6)-carboxyfluorescein (5(6)-FAM)) (Sigma-Aldrich). Finally ...
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bioRxiv - Immunology 2023Quote: ... and maintained at 37°C with 5% CO2 in complete RPMI in the presence of 5% human AB serum (Sigma-Aldrich) for differentiation into MDMs for 10-14 day (Pierini et al. ...
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bioRxiv - Biophysics 2024Quote: Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate (cAMP) and guanosine 3’,5’-cyclic monophosphate sodium salt (cGMP) were purchased from Sigma Aldrich and both prepared at 16 mM in 150 mM KCl ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ug protein samples were boiled at 90°C for 5 minutes in LDS sample buffer (Genscript#M00676 or Millipore#MPSB) containing 5% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 5 units/μl of DNA polymerase in diluted Polymerase Buffer with Red Fluorescent-labeled Oligonucleotides (1:5, Sigma Aldrich) for 100 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Unspecific antibody binding was blocked by incubating the blots in 5 % non-fat milk or 5 % bovine serum albumin (BSA, #A9647, Sigma-Aldrich) in 1x Tris-buffered saline with 0.1 % Tween 20 (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... and the recorded transients were characterized using the same software.65,66 Electrodes were calibrated using background-subtracted cyclic voltammograms obtained with 1 μM 5-HT solution diluted in PBS (5-HT; Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... was obtained from Sigma-Aldrich and used at a concentration of 5 μM. 5-(N-Ethyl-N-isopropyl)amiloride (Cat no. A3085-25MG) was obtained from Millipore Sigma and was used at a concentration of 25 μM ...
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bioRxiv - Synthetic Biology 2024Quote: ... was generated from Venus BBa_J176006 with primers 5’-tctgcacctgaggccaccatggtgagcaagggcgagg and 5’-ggtcacgaattccagcaggaccatgtgatcg via high fidelity PCR followed by spin-column purification (NEB #E0555, Sigma #NA1020). The YFP fragment and mutated pSBtet-GP plasmid were double-digested with Eco81I/ EcoRI (Thermo #FD0374 ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Immunology 2024Quote: ... Mice were monitored daily from day 5 onwards by taking a thin blood film and staining with 5% Giemsa (Sigma Aldrich) to screen for the presence of schizonts within the red blood cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in 5 ml 10% fetal bovine serum/PBS and fixed by adding 5 ml 4% formaldehyde (Sigma-Aldrich) at a final concentration of 2% ...
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bioRxiv - Immunology 2024Quote: ... The cells were cultured at 37°C in a 5% CO2 incubator for 6 days using Corning RPMI 1640 supplemented with 5% fetal bovine serum (Sigma-Aldrich) and 5% penicillin-streptomycin (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... lung or intestinal tissue were fluorescently labeled via reductive amination with 2-aminobenzamide (2-AB) (Sigma-Aldrich Canada)42 ...
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bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
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bioRxiv - Bioengineering 2022Quote: ... and antimycin A (AA) (500 nM) and 2-deoxy-D-glucose (2-DG) (25 mM, all Sigma-Aldrich) were prepared in XF assay medium and loaded into the appropriate injection ports on the cartridge plate and incubated for ten minutes in a non-CO2 incubator at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5 µL g-1 solution of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) was added at 4°C temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM lipids: POPC: POPE: cardiolipin with a 2:2:1 weight ratio) and 160 μL MSP1D1 (Sigma, 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in ice cold HBSS (2% serum) containing 2 μg/ml Propidium iodide (Sigma-aldrich,USA) for dead cell discrimination ...
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bioRxiv - Cell Biology 2020Quote: ... TEBP-1-His5 and TEBP-2-His5 were expressed in Rosetta 2 (DE3) pLysS Competent Cells (Novagen,#71401). An overnight culture was grown in LB containing the respective antibiotic ...
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bioRxiv - Biophysics 2020Quote: ... Cystamine (Cys) and N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES ≥ 99.5 %) were also purchased from SIGMA. All samples were prepared in 0.1 M HEPES buffer ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 μL g−1 hydrogel of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) were mixed in at a temperature of 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 2 ml of PBS with 2 mM EDTA and 25 μM cisplatin (Sigma-Aldrich, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Microbiology 2022Quote: ... 150 μM (2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5 compound; Sigma-Aldrich, EF5014), 0.5 μM MitoSOX™ fluorescent probe (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... the noradrenergic neurotoxin N-(2-Chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4) (10 mg/kg, i.p., Sigma-Aldrich) or vehicle (0.9% saline ...
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bioRxiv - Biochemistry 2022Quote: ... also contained 1-2 µg/uL α-MBPAF546 or 1-2 µg/uL mouse α-BRCA2 (Ab1, Millipore) plus 1-2 µg/µL goat α-mouse IgGAF546 (Molecular Probes).
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bioRxiv - Molecular Biology 2023Quote: Metabolic quenching and nucleotide phosphate metabolite pool extraction were performed by adding 1 ml ice-cold 2:2:1 acetonitrile:methanol:water with 0.1% formic acid with 1x MS-Stop phosphatase inhibitor (Sigma-Aldrich). Samples were spiked with 10 µl 100 mM mix of adenosine-15N5-5′-triphosphate ...
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bioRxiv - Bioengineering 2023Quote: ... in 100 mM HEPES buffer (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid, pH 5.25, Sigma, St. Louis, MO) mixed with 3.5 kDa PEG-DT (JenKem ...
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bioRxiv - Microbiology 2023Quote: ... Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) and 2-chloroacetamide (CAA) used for proteomics analysis were from Sigma Aldrich. The Bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... N=4 (2 females, 2 males) mice were given 12.5 mg/kg sodium nitrite (52mg/L, 23713, Sigma) in 10% blackcurrant squash (Robinsons) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were induced for Ngn2 and DjCas13d expression using 2 ug/mL Doxycycline (2 ug/mL, Sigma D3072). On day 3 ...
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bioRxiv - Neuroscience 2024Quote: ... The explants were transferred within 2 h to 200 μl DMEM containing 2% FA-free BSA (Sigma-Aldrich) and incubated for 1 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... phosphatase inhibitor cocktail 2 (Sigma-Aldrich; P5726) and phosphatase inhibitor cocktail 3 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5% Phosphatase inhibitor cocktail 2 (Sigma-Aldrich). MAPK phosphorylation was determined using an anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biophysics 2021Quote: ... Zn(NO3)2 (0.5 mM; Sigma-Aldrich), Mg(NO3)2 (0.5 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% 2-mercaptoethanol (Sigma Cat#M-7522), 500,000U Recombinant Mouse LIF Protein (Millipore Cat#ESG1107) ...
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bioRxiv - Biochemistry 2021Quote: ... coli Rosetta 2 (DE3) cells (EMD Millipore) was inoculated into 1 liter of LB medium containing ampicillin (50 μg/ml ...
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bioRxiv - Biophysics 2020Quote: ... 2% MEM amino acids (50x, M5550, Sigma), 1% Penicillin Streptomycin (10,000 units/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (G7513, Sigma-Aldrich), and penicillin/streptomycin (P0781 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (G7513, Sigma-Aldrich), and penicillin/streptomycin (P0781 ...
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bioRxiv - Cancer Biology 2021Quote: ... blasticidin (2 µg/mL) (Millipore Sigma, 15205) selection was performed ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 2 U/ml Heparin (Sigma), 0.1 μg/ml Hydrocortisone (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... and reduced with 2-Mercaptoethanol (Sigma-Aldrich). Protein lysates were boiled for 15 min at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... and 2% β-mercaptoethanol (Sigma-Aldrich #63689) were boiled at 99°C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... coli Rosetta™ 2 (DE3) pLysS (Novagen). Cells were grown in LB-Luria at 37□°C and 160□rpm to an OD600 of 0.6–0.8 ...
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bioRxiv - Cell Biology 2020Quote: ... sodium-3-methyl-2-oxobutyrate (ketovaline, Sigma), 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine ...
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bioRxiv - Cell Biology 2020Quote: ... then supplemented with 2 mM Thymidine (Sigma). Cells were released 16 h later by washing 2 times with prewarmed WT medium ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM EDTA (#ED-1KG, Sigma, Austria), 50 mM NaF (#SO0323 ...
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bioRxiv - Cell Biology 2020Quote: ... auxin (2 mM, Sigma Aldrich I5148-10G) CuSO4 (200 μM ...
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bioRxiv - Cell Biology 2020Quote: ... Phosphatase Inhibitor Cocktail 2 (Sigma, Cat. P5726), cOmplete Protease Inhibitor Cocktail tablets (Roche ...