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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Protein DBF4 Homolog A DBF4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: ... Following the incubation with a secondary antibody with a linked horse radish peroxidase the proteins were visualized by applying Immobilon™ Chemiluminescent Substrate (Millipore) and detection on an X-ray film.
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bioRxiv - Genetics 2019Quote: Cell extracts were prepared by TCA precipitation and proteins were separated by 10% SDS-PAGE followed by immunoblotting with anti-HA antibody (Sigma-Aldrich, ref ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with primary antibodies for overnight at 4°C and then protein A/G magnetic beads (Millipore, USA) were added for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... on genomic DNA isolated from 3-week-old tissue-cultured plants as a template and then selected by northern blot analysis with a HT specific cDNA fragment as probe and subsequently by western blot analysis with antibodies specific to rat HT protein (Sigma-Aldrich).
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were adjusted to 1 mg/ml in lysis buffer and subject to overnight immunoprecipitation at 4°C with either USP7 or USP11 antibody and protein A agarose beads (Sigma-Aldrich), or with GFP-NanoTrap beads (prepared in house ...
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bioRxiv - Plant Biology 2020Quote: ... the FLAG-tagged proteins were immunoprecipitated by incubation of the extracts with 5 μL of anti-FLAG antibody agarose beads (Sigma-Aldrich) for 1 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Puromycin-labeled proteins were detected either by Western blotting or by indirect fluorescence microscopy using a monoclonal anti-puromycin antibody (12D10; Sigma Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged proteins were probed with immunoblotting using monoclonal anti-Flag M2-Peroxidase (HRP) antibody (Sigma-Aldrich, Saint Louis, MO, USA) (1/50,000X dilution ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to a PVDF membrane and incubated with one of the following primary antibodies: α-FLAG polyclonal rabbit (Sigma) or α-RpoA monoclonal mouse (Biolegend) ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... 30 confirmed transgenic plants were screened for dPPRrbcL expression by immunoblot on leaf total protein extracts using HA antibodies (H9658 clone, Sigma-Aldrich) and three lines expressing gradual levels of dPPRrbcL protein were chosen for phenotypic analyses.
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... to preclear samples for 2 hours and subsequently incubated with Dynabeads protein G coated with an anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2022Quote: ... Chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated with the indicated antibodies or rabbit preimmune sera complexed with Protein A agarose beads (Sigma-Aldrich). Immunoprecipitated complexes were sequentially washed with Wash Buffer A ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Cell Biology 2022Quote: ... Hxk2-3V5 and associated proteins were immunoprecipitated from glass bead extracts (250 μg total protein) using 20 μL of agarose conjugated anti-V5 antibody (Sigma; A7345). The extract and beads were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The samples for mass spectrometry (MS) analysis were incubated with Dynabeads protein G that were precoupled with the M2-Flag-antibody (Sigma, F1804) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...