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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Protein DBF4 Homolog A DBF4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The expression of the proteins was assessed by western blot using antibodies raised against the T7 epitope (Novagen) and GAPDH (Abcam).
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bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
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bioRxiv - Neuroscience 2023Quote: ... the primary antibody was glial fibrillary acidic protein (GFAP) (1:1000 dilution; Millipore, Temecula, CA, USA; Cat. #MAB360). Secondary antibodies were conjugated to Alexa Fluor 594 (donkey anti-mouse ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore, Darmstadt, Germany, AB5543, 1:1000) and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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bioRxiv - Genetics 2023Quote: ... The dilution ratios were as follows: 1:1000 for antibodies to kinetochore proteins and 1:100 for antibodies to α-tubulin (Sigma-Aldrich, St. Louis, MO; catalog number T6199). After washing twice for 5 minutes in 1× PBS at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes and immunoblot analyses were carried out using rabbit antibodies directed against ShcA (Millipore), Collagen X (Abcam) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2021Quote: ... and the expression of the bait-protein was detected from the input sample with anti-FLAG antibody (Sigma Aldrich).
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Argonaute proteins/small RNA complex were extracted from 50,000 synchronized worms and immunoprecipitated with anti-flag antibody (Sigma Aldrich) or GFP nanobody (Thermo) ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was subjected to an immunoreaction with a specific antibody pre-conjugated with magnetic protein A beads (Millipore) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2019Quote: ... The collected lysate proteins were then subjected to immunoblotting with LC3 (L7543) and β-actin (A5441) antibodies (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... proteins were transferred to a PVDF membrane and immunoblotted with an anti-phosphotyrosine antibody (1:1000 dilution, Upstate/Millipore) for chemiluminescence detection.
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bioRxiv - Plant Biology 2020Quote: ... The expression of the target proteins was verified by western blot using ANTI-FLAG M2 Monoclonal Antibody (Sigma-Aldrich). ChIP assay was performed with ANTI-FLAG M2 Magnetic Beads (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were detected with antibodies after western blotting: ABCE1-TAP with peroxidase anti-peroxidase (PAP) complex (Sigma-Aldrich) at 1:2,000 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Histone H3 lysine 27 trimethylated protein was detected using an anti-H3K27me3 antibody (rabbit polyclonal, Millipore, Burlington, MA, USA) diluted 1:500.
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Biochemistry 2022Quote: ... then complexes immunoprecipitated using the monoclonal antibody 13D11 against the B-subunit followed by Protein A Sepharose beads (Sigma). After elution with cracking buffer ...
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Local induction of IAA5 and IAA29 promotes DNA damage-triggered stem cell death in Arabidopsis rootsbioRxiv - Plant Biology 2022Quote: ... Chromatin bound to SOG1-Myc proteins extracted from whole seedlings was precipitated with anti-Myc antibody (clone 4A6, Millipore). To quantify the precipitated chromatin ...
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bioRxiv - Molecular Biology 2022Quote: ... The filters were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (07-181, Millipore), p-AMPK (#2535 ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein was used for immunoblot analysis using an anti-HA antibody (1:1000 dilution, Sigma) and anti-cMyc antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Protein labeling was done by incubation with appropriate primary antibodies as indicated (Supplementary Table S1) in 5% BSA (Sigma) at 4°C overnight ...
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bioRxiv - Genomics 2022Quote: ... SPA-tagged protein was pulled down from clarified lysates with an anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) bound to Protein A-Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...