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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Protein DBF4 Homolog A DBF4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 4-5 days after transfection the antibodies were purified from the supernatant by protein A affinity resin (ProSep®-vA ultra, Millipore®, Burlington, MA, USA) and dialyzed into phosphate buffered saline pH 7.2 (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with antibodies against selected proteins overnight at 4°C: Anti-β-Tubulin III (β-Tub III) (Sigma, T8660, mouse, 1:2000); anti-p-Tau (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The loading consistency was verified utilizing the house-keeping proteins (Rab11 and β-Actin) by stripping and re-probing blots with monoclonal β-actin primary antibody (Sigma, St Louis, MO) following the similar procedure as described earlier.25 ...
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bioRxiv - Genetics 2022Quote: ... the membrane was probed with the primary antibody overnight at 4°C (rabbit anti-beta galactosidase antibody, 15518-1-AP Protein Tech, dilution 1/1000: mouse anti-alpha-tubulin, T6199 Sigma-Aldrich, dilution 1/10 000) and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
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bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein was treated with SUMO protease (Sigma SAE0067, 5 U/ mg target protein) to liberate Mpro with authentic N-(Ser1 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285) and intact cell respiration was normalized to milligrams of total intact cell protein.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285).
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bioRxiv - Cell Biology 2019Quote: Primary antibodies: Sept2 (SIGMA Atlas Antibodies HPA018481), GFP (Roche 12600500) ...
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bioRxiv - Microbiology 2019Quote: ... antibody and rabbit anti-Flag antibody (Sigma) solution for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.
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bioRxiv - Cell Biology 2022Quote: ... and the protein concentration of the supernatant assessed by Pierce 660nm protein assay reagent (Sigma, 22660). All samples were diluted to a final 1X Laemmli sample buffer (40% glycerol ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined in the supernatants using Bradford protein assay method (Bradford reagent, Sigma–Aldrich), a standard curve was performed using BSA (bovin serum albumine ...
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bioRxiv - Biophysics 2023Quote: ... The fusion protein was treated with SUMO protease (Sigma-Aldrich SAE0067, 5 U/ mg target protein) to liberate 3CLpro with authentic N- and C-termini (Ser1 and Gln306 ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Physiology 2023Quote: ... The total protein content in samples was obtained using a BCA protein assay kit (Sigma-Aldrich) with the SpectraMax Paradigm microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... 9 mg of protein were mixed with 20 µL of S-protein Agarose beads (#69704, Novagen), that had been previously washed in extraction buffer with 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... After determining the protein concentration through a Bradford protein assay using Bradford reagent (Sigma, cat# B6916), each sample was diluted with an appropriate volume of RIPA buffer mixed with 1x Halt protease & phosphatase inhibitor cocktail to achieve a final protein amount of 20 µg protein ...
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bioRxiv - Neuroscience 2024Quote: ... For non-neuronal marker (GFAP antibody: Millipore AB5541; Iba1 antibody: GeneTex GTX101495; Sox9 antibody: RnDsystems AF3075; S100β antibody: Sigma S2532), antibodies were diluted at 1/500.
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bioRxiv - Molecular Biology 2019Quote: ... Protein A Sepharose beads (GE17528001; Sigma) blocked with 1mg/mL BSA (10848 ...