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Citations for Millipore Sigma :
5351 - 5400 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA with freshly added 1 mM DTT), then concentrated to 500 μl using Amicon Ultra-4 (4 mL, 3 kDa cut-off) (Millipore, Darmstadt, Germany, UFC800324) and loaded onto a Superdex75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: Male CD-1 mice aged 6-10 weeks were given an ip injection of 3 mg/kg LPS from Salmonella typhimurium (Sigma, St. Louis, MO) dissolved in sterile normal saline at 0 ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended again in 1x PBS (pH□=□7.4) with 3□mg□mL−1 of urease powder from Canavalia ensiformis (Jack bean) (Sigma-Aldrich cat. no. U4002). When fabricating micromachines with peptides attached ...
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bioRxiv - Plant Biology 2020Quote: ... Then samples were incubated for 1 hour and 15 minutes in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (item 03450, Sigma-Aldrich, St. Louis, MO) solution ...
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bioRxiv - Cell Biology 2022Quote: 12- week-old C57BL/6 male mice were injected intraperitoneally with CCl4 (HPLC grade, Cat#270652, Sigma, diluted 1:3 in mineral oil) or mineral oil (control ...
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bioRxiv - Neuroscience 2020Quote: ... These free-floating sections were blocked with 1% normal goat serum for 1 hour in 0.01M phosphate buffered saline (PBS) before incubation with with a mouse α-NeuN (Fox-3) antibody (1:1000; Millipore, Cat#: MAB377 (2018)) and a rabbit α-TDP-43 antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and blocked for 1 h at RT with a serum containing 3% bovine serum albumin (BSA; Sigma−Aldrich, cat. no. A9647), 10% goat serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue sections where fixed in -20 °C methanol for ten minutes followed by a blocking cycle (5×3 min) using phosphate buffered saline containing 1% bovine serum albumin (Sigma-Aldrich, Copenhagen, Denmark). The samples where then incubated overnight at 4 °C with the primary antibodies (dilution factor ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000, EMD Millipore, #AP132B, RRID:AB_11212148) followed by 1 h with avidin-biotin complex (ABC Vector Laboratories ...
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bioRxiv - Zoology 2020Quote: ... The attractant blend was delivered via an impregnated 3 cm cotton dental place inside a 1 ml Eppendorf® tube (Sigma-Aldrich, USA). The open tube was then attached to the inside chamber of the trap ...
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bioRxiv - Developmental Biology 2020Quote: Tadpoles were anesthetized at room temperature by incubation for 30-60 seconds in a 1:2000 dilution of MS222 (ethyl 3-aminobenzoate methanesulfonate, Sigma, St. Louis, MO) diluted in 1/20X Normal Amphibian Media ...
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bioRxiv - Genomics 2021Quote: ... Samples were centrifuged at 500 rcf for 1 min and the supernatant was replaced by 50 μL of dispase (3 mg/mL, Sigma-Aldrich, D4818, 2mg) and 75 μL of collagenase I (100 mg/mL ...
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bioRxiv - Genetics 2022Quote: ... then incubated overnight with appropriate primary antibodies (Supplementary Table 3) in antibody solution consisting of 1xPBS with 1% BSA (Millipore Sigma A4161-1G) and 0.3% Triton X-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Immunology 2022Quote: ... were dosed 2 mL/Kg intraperitoneally (IP) twice weekly with either olive oil (N=8) or CCl4 (CCl4:olive oil, 1:3, [v:v], Sigma-Aldrich, St Louis, MO) for 4 weeks ...
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bioRxiv - Microbiology 2022Quote: ... a 50:50 mixture of ALI x2 media (Airway Epithelial Cell Basal Medium with 2 supplement packs added (without triiodo-L-thyronine and retinoic acid supplements) and 1 ml BSA (3 µg/ml)) and DMEM supplemented with retinoic acid (15 ng/ml) (Sigma Aldrich, Gillingham, UK). Cells were fed apically and basolaterally until 100% confluent ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... with 100 mM NaCl and 1 mM DTT and concentrated to 20-60 mg/mL by ultrafiltration using a 3-kDa cutoff filter (Millipore-Sigma, Burlington, MA).
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bioRxiv - Genomics 2024Quote: ... The blood was diluted with PBS at a 1:3 dilution and underlaid with Histopaque®-1077 (Sigma-Aldrich; Cat. No. 10771-100ml). The underlaid blood samples were centrifuged (TECHCOMP CT1SRT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Microbiology 2023Quote: ... 0.165 M 3-(N- morpholino)propanesulfonic acid (Melford, Suffolk, UK) adjusted to pH 7 by 1 M NaOH (Sigma-Aldrich, Burlington, MA, USA)) ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were centrifuged for 7 minutes at 2000rpm for higher transfection efficiency and were incubated for 1-3 days with coving by Breathe-Easy sealing membrane only (Sigma-Aldrich, # Z380059-1PAK). All assays were performed on Day 1-3 following transfection ...
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bioRxiv - Microbiology 2023Quote: ... and probed with the appropriate primary antibody diluted in TBS-T with 3% BSA: rabbit anti-HA (Sigma Aldrich #H6908; 1:3,000 dilution), mouse anti-FLAG (Sigma Aldrich #F1804 ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Neuroscience 2023Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with 0.05% Tween-20 in phosphate buffered saline (PBST) for 3 × 5 min with agitation and incubated with anti-β actin (1:1000 rabbit polyclonal (Sigma Aldrich; A2066) or 1:5000 mouse monoclonal (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... SP3 beads were then resuspended in 100 μL of 200 mM HEPES (pH 7.5) and digested overnight at 37 °C with Solu-trypsin (3 µg solu-trypsin, Sigma, trypsin:protein ratio 1:33). The resulting peptide mixtures were collected using a magnetic rack ...
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bioRxiv - Genomics 2023Quote: ... The membrane is washed again with 1x TBST solution, and then incubated the primary antibody solution (3% BSA in TBST, 1:250 anti-HA (Sigma-Aldrich, A2095-1ML), 1:500 anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were blocked in 1% H2O2 and 3% bovine serum albumin (BSA, Cat. No. D5637, Sigma-Aldrich, St. Louis, Missouri, USA). Pons sections were stained with Hematoxylin and Eosin (H&E ...
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bioRxiv - Plant Biology 2024Quote: ... medium lacking Trp but containing 40 μg mL-1 5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside (X-α-gal; Sigma‒Aldrich) at 30°C for 3 days ...
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bioRxiv - Immunology 2024Quote: ... The aorta was dissected circumferentially away from the surrounding tissues and subjected to 3 minutes of either 5 µL of peri-adventitial elastase (0.4 U/mL type 1 porcine pancreatic elastase, Sigma Aldrich, St. Louis, MO) or heat-inactivated elastase as control ...
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bioRxiv - Neuroscience 2024Quote: ... every third consecutive vibratome brain tissue section containing medial septal nucleus (MSN) was treated with 3% H2O2 before blocking and then incubated with an anti-ChAT antibody (Millipore Sigma; 1:250) as described previously (Jiang et al. ...
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bioRxiv - Biophysics 2024Quote: ... 1 eq of nonlabelled PDZ was buffer exchanged to the NMR buffer using a 3 kDa centrifuge filter (Millipore Sigma, St. Louis, MO) and directly added to the monomer.
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bioRxiv - Bioengineering 2024Quote: ... the apical and basal PDMS parts were plasma treated and incubated in freshly made 1% (3-glycidyloxypropyl)trimethoxysilane (Sigma-Aldrich, Cat. no. 440167) in deionized water for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were blocked with 3% BSA for 20 min at RT before incubation with a monoclonal anti-FLAG-M2-FITC (Sigma, F4049, 1:500) or anti-HA-FITC antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3 times with PBST and 100 μL of o-phenylenediamine dihydrochloride (SigmaFast OPD, Sigma) substrate was added per well ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3 times with PBST and 100 µL of o-phenylenediamine dihydrochloride (SigmaFast OPD, Sigma) substrate was added per well ...
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bioRxiv - Cancer Biology 2021Quote: ... PreScission protease-eluted supernatant was added to 100 mL of S-protein agarose (EMD Millipore, 69704-3) to capture S-tagged protein ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated muscles from female C57BL/6J mice were pre-incubated with 40 µM IPA-3 (Sigma-Aldrich) or a corresponding amount of DMSO (0.11% ...
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bioRxiv - Genetics 2021Quote: ... then were stimulated with either 10 nM DHT (5α-Androstan-17β-ol-3-one, Dihydrotestosterone, A8380, Sigma) or EtOH (Vehicle ...
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bioRxiv - Developmental Biology 2021Quote: Zona pellucida were removed from embryos with 3-4 min pronase (0.5% w/v Proteinase K, Sigma P8811 ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were anesthetized with Ethyl 3-aminobenzoate methanesulfonate salt (MS-222, Tricaine, Sigma-Aldrich, St. Louis, MO) and adults were euthanized by rapid cooling as previously described (Wilson et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... After three washes (15 min) with PBS supplemented with 3 mg/ml poly-vinylpyrrolidone (PVP) (Sigma, P0930), embryos were permeabilized with PBS containing 5% DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich; Cat. No. A7906) in PBST ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed with 1X PBS and embedded in 3% agarose Type I-B (Sigma-Aldrich, St. Louis, MO) dissolved in 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Molecular Biology 2021Quote: ... a 500 μl 50% suspension of 3 times pre-washed streptavidin agarose beads (Novagen, Cat. N°69203) (i.e. ...
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bioRxiv - Molecular Biology 2021Quote: A CU RNA hairpin (5’-AAAAGAAAAGACGCGUAGUUUUCUACGCG- 3’) labeled with Cyanine 5.5 at the 5’-end (Millipore Sigma) was annealed in 20 mM HEPES ...
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bioRxiv - Genomics 2020Quote: ... RNA was degraded by the addition of 3 μL of 33 mg/mL RNase A (Sigma R4642) and incubation at 37°C for 2 hours ...