Labshake search
Citations for Millipore Sigma :
5301 - 5350 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Cells were typically seeded at a density of 1-3 × 105 cells/cm2 on dishes coated with poly-L-lysine (Sigma-Aldrich, P1524). Neuronal cultures were treated overnight in 1 μM tetrodotoxin (TTX ...
-
bioRxiv - Neuroscience 2022Quote: ... in addition to 10% FBS and 1% PS we supplemented the medium with 3 μg/ml puromycin (Sigma Aldrich, cat. no. P8833). For the experiments that involved transfection and electrophysiological recordings ...
-
bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
-
bioRxiv - Cancer Biology 2020Quote: ... For dissociation to single cells the tissue was minced using scissors and incubated with enzymatic digestion solution containing 3 mg ml-1 collagenase A (Sigma Aldrich, 11088793001) and 70 unit ml-1 DNase in RPMI 1640 (Biological industries ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
-
bioRxiv - Genomics 2021Quote: ... The dried samples were reconstituted using a buffer solution (0.1% formic acid: acetonitrile = 97:3) and was then filtered through a 0.45 μm centrifuge filter (Millipore; 1 min, 13,000 rpm). The supernatant was then transferred to a 96-well collection plate ...
-
bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
-
bioRxiv - Cell Biology 2020Quote: ... This basal medium was supplemented with 2i-LIF (3 µM CHIR99021, Sigma-Aldrich, cat. no. SML1046 and 1 µM PD0325901 Sigma-Aldrich, cat ...
-
bioRxiv - Biochemistry 2021Quote: ... Dried supernatant was dissolved in 1 ml of 5 mM ammonium bicarbonate and purified on a 3 ml porous graphitic carbon column (EnviCarb; Sigma Aldrich – Supelco) equilibrated with 2 ml of 80 % acetonitrile solution with 0.1 % TFA and 2 ml of water ...
-
bioRxiv - Molecular Biology 2021Quote: ... with 1:100 dilution of GlutaMax and penicillin/streptomycin and cells were treated (n = 3 per group) with compounds and tBHQ (Sigma 11294-1) for 24 hr ...
-
bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were spin-infected at a 0.3 multiplicity of infection in the presence of DMEM (10% FBS, 1% L-Glutamine) and hexadimethrine bromide (8ug/ml) (Sigma-Aldrich, 107689) at 2000 rpm ...
-
bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
-
bioRxiv - Physiology 2024Quote: ... The rate of pH change was measured every 13 minutes for a 3-minute interval before sequential challenge with 1) 20mM glucose (Sigma Aldrich D9434), 2 ...
-
bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... with 100 mM NaCl and 1 mM DTT and concentrated to 20-60 mg/mL by ultrafiltration using a 3-kDa cutoff filter (Millipore-Sigma, Burlington, MA).
-
bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 3 hours with 1 µg/mL lipopolysaccharides (LPS) from Escherichia coli O111:B4 (Sigma-Aldrich #L2630; Lot #000110081) in 0.25%(w/v ...
-
bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... then incubated in 1xPBS + 0.5% Tritton X-100 + 3% Bovine Serum Albumin before adding the primary antibody (HPA036814, Sigma-Aldrich – 1/1000) for 48h at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... these cultures were diluted to a starting OD600 of 0.01 in 3 mL of BHIS supplemented with increasing concentrations of 1) bile salt mixture (50% cholate and 50% deoxycholate; Sigma-Aldrich B8756), 2 ...
-
bioRxiv - Immunology 2022Quote: ... Wells were then washed three times with 0.05% Tween 20 in PBS and blocked for 1 hour at room temperature with 100 μL of 3% globulin free BSA (Sigma-Aldrich, A7030) in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... both HeLa cells and cortical neurons were permeabilized after PFA fixation with 100% methanol chilled at −20℃ for 20 minutes followed by 1 h blocking with PBS containing 3% BSA (Sigma-Aldrich; A9418). Cells were stained with antibodies against SIN capsid ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 times with D-PBSX before incubation with the corresponding secondary antibodies (all diluted 1:500) and DAPI (Sigma, #32670-25mg) for 1 h (RT) ...
-
bioRxiv - Cell Biology 2023Quote: ... for 3 h in serum- free RPMI-1640 (with 10,000 U/mL Penicillin, 10 mg/mL Streptomycin, 1:100) (Sigma-Aldrich, Sydney, Australia) at a density of 0.2 × 106 cells/cm2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with PBS and DNA content were visualized by DAPI (1 μM; Cat# D9542, Millipore Sigma, I h) staining at room temperature ...
-
bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
-
bioRxiv - Developmental Biology 2023Quote: ... animals were amputated to induce Erk activation and then dissociated at 3 hpa in CMF supplemented with a 1:10 dilution of Liberase TH (Millipore Sigma, 5401135001) into single cells (Witchley et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... with secondary antibody in PBST containing goat serum, washed (3 × 5 min), stained (10 min, 20°C) with DAPI (1 µg/ml in PBS; Sigma, Cat# D9542) and washed (5 min ...
-
bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
-
bioRxiv - Cell Biology 2023Quote: ... 24 hours post-knockdown and induction coverslips were fixed in -20oC methanol for 3 minutes and stained for ms-gamma tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig CENP-C 1:25 (MBL ...
-
bioRxiv - Cell Biology 2023Quote: ... Cover glasses were then activated by silanization with an ethanol solution supplemented with 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... and stained for 15 minutes in Alcian Blue solution (1% Alcian Blue in 3% solution of acetic acid) (Cat. # A5268, Sigma Aldrich, UK). Slides were then washed in running tap water for 2 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G ...
-
bioRxiv - Plant Biology 2024Quote: ... and combined with 0.2 mL of a 3 mg L-1 TAG C15:0 solution (Tripentadecanoin, reference T4257, Sigma-Aldrich™, USA) in anhydrous hexane 95 % (296090 ...
-
bioRxiv - Pathology 2024Quote: ... Membranes were washed with TTBS 3 times for 5 min each and incubated in HRP-conjugated anti-rabbit (1:5,000; Sigma-Aldrich, catalog #SAB3700843) or anti-mouse (1:10,000 ...
-
bioRxiv - Neuroscience 2024Quote: ... and then incubated them overnight in a primary antibody for phospho-Histone 3 (pH3, Millopore-Sigma, H0412, made in rabbit, 1:1000) in blocking buffer at 4°C with gentle rotation ...
-
bioRxiv - Microbiology 2024Quote: ... serial 1:10 dilutions of virus samples were made in gel saline (3 mg/mL gelatin from porcine skin (Sigma Aldrich, #G2625) in DPBS (Gibco ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 30 min followed by incubation with 1 mM 8-bromoadenosine 3’,5’-cyclic monophosphate (8-Br-cAMP, Sigma, St Louis, MO) for 15 min ...
-
bioRxiv - Plant Biology 2024Quote: ... wetted with 2 mL of liquid ½ MS medium at pH 5.4 and supplemented with 3% sucrose and 100 µg·mL-1 acetosyringone (Millipore sigma, Burlington, MA, #D134406). Plates were sealed with Parafilm (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... slices were pre-incubated with BioTracker NuvView® Blue Caspase-3 Dye for >30 minutes (Sigma Aldrich, 5μL dye:1 mL media). Two-photon (2P ...
-
bioRxiv - Cell Biology 2020Quote: ... Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (Sigma). The reaction was allowed to proceed for 1 hour ...
-
bioRxiv - Neuroscience 2022Quote: ... after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... This was followed by 1-hour incubation at RT with the diluted secondary antibody after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections were captured on pre-labeled 1×3 mm glass slides coated with 10% poly-L-lysine (Sigma Aldrich, St. Louis, MO, USA) and maintained at 37° during sectioning ...
-
bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
-
bioRxiv - Neuroscience 2020Quote: ... sectioned organoids were washed three times with 1x PBS for 10 minutes at RT before blocking for 1 hour with 3% bovine serum albumin (BSA) (Sigma, Cat. A9418-50G) and 0.1% triton X-100 in 1x PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice with 0.1% Triton-X in PBS and blocked with 3% BSA (constituted from powder BSA, Roche Fraction V, sold by Sigma Catalog Number: 10735078001) in PBS for 30 minutes at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... Then samples were incubated for 1 hour and 15 minutes in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (item 03450, Sigma-Aldrich, St. Louis, MO) solution ...