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Citations for Millipore Sigma :
4951 - 5000 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 0.05% Triton X-100) supplemented with 1/500 (v/v) plant-specific protease inhibitor cocktail and phosphatase inhibitor cocktail 3 (Sigma-Aldrich). 20 μL (bed volume ...
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bioRxiv - Microbiology 2021Quote: Metabolites were extracted from cell pellets through addition of 50 μl chloroform and vigorous vortexing, followed by 200 μl methanol/ultrapure water (3:1, containing 5 μM scyllo-inositol (Sigma I8132) as internal standard ...
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bioRxiv - Molecular Biology 2021Quote: Exponentially growing Jurkat Tat KB001 or mdHIV clone 11 cells (3×107 cells/IP) were fixed with 1% formaldehyde (Sigma-Aldrich) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated for 24 hours with either 1 mM 3-Hydroxy-1,2-dimethyl-4(1H)-pyridone (Deferiprone/DFP, Sigma-Aldrich, 379409), or incubated in Earl’s balanced salt solution (EBSS ...
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bioRxiv - Cell Biology 2021Quote: ... validated lentiviral constructs (pLKO.1) expressing Mission shRNA targeting the following region 5’-GCTAGATTTACTAATGCCA-3’ in CPAP or scrambled shRNA were purchased from Sigma-Aldrich. For generation of stable cells ...
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...
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bioRxiv - Developmental Biology 2022Quote: ... RNAs immersed in Trizol were added to phase-lock heavy gel tubes (Brinkmann Instruments, INC., #955-15-404-5) and mixed with 100 uL of 1-bromo-3-chloropropane (BCP) (Sigma, #B9673), followed by room temperature incubation for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Ø=3 mm) and the dura carefully removed before covering the brain surface with 1% agarose (type III-A; Sigma-Aldrich) and artificial CSF (in mM ...
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bioRxiv - Cell Biology 2022Quote: ... Printed discs were incubated in 0.1 mg/mL FITC-labelled dextran molecules (size either 3-5 kDa, 10 kDa, 20 kDa or 70 kDa (Sigma-Aldrich)) dissolved in PBS with 20mM CaCl2 (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... Cover glasses were then activated by silanization with an ethanol solution containing 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed 3 times with PBS and mounted on a microscope slide with parafilm gasket in 1% (v/v) β-mercaptoethanol (Sigma), 150 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced under the microscope after 3 hours with fresh L15 media containing 10% FBS with a mock solution (1/1000 of DMSO - Sigma D8418), 10μM Nutlin-3 (Sigma N6287) ...
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bioRxiv - Biophysics 2021Quote: ... for 1 hour at room temperature and washed 3 times with PBS-T before mounting in 1% low melting point agarose (T9284, Sigma-Aldrich) followed by light sheet imaging ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Neuroscience 2022Quote: ... with nerves from 3 mice pooled per time point in ice-cold PBS with 1% protease inhibitor cocktail (Sigma-Aldrich, P8340). Samples were minced ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 3% BSA in PBS-T buffer for 1 h at room temperature followed by incubation with Streptavidin-HRP (Sigma-Aldrich) (1/25,000 ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Neuroscience 2021Quote: ... Then the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL, Sigma-Aldrich). Images were acquired using a Zeiss 780 inverted confocal microscope ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μL of the overnight cultures were diluted into 3 mL of LB containing 25 μg/mL chloramphenicol and 1 mM IPTG (Sigma-Aldrich) and incubated at 37°C with shaking until early stationary phase (OD600 0.7-1.1).
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bioRxiv - Physiology 2021Quote: ... Five μL of each dilution were introduced into the AscentisSi column (10 cm × 1 mm, 3 μm, Sigma-Aldrich, Darmstadt, Germany) with a flow rate of 50 μL min−1 ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked with 3% BSA/TBST for 30 min and incubated with rabbit anti-Sox9 polyclonal antibody (1:500, EMD-Millipore, AB5535), rat anti-endomucin (Emcn ...
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bioRxiv - Immunology 2021Quote: ... cells were removed and plates were incubated with biotinylated detection antibodies and Streptavidin-HRP conjugate following the manufactures introductions using a 1 in 100 dilution for the streptavidin-HRP conjugate with 3-Amino-9-ethyl-carbazole (AEC; Sigma-Aldrich) dissolved in N,N-dimethylformamide (Merck Millipore ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000073737 5’-CCGGCGCGTTATCAACTGGATCCAACTCGAGTTGGATCCAGTTGATAACGCGTTTTTG-3’ designed and cloned into the lentiviral pLKO.1 puromycin resistant vector Mission shRNA lentiviral Transduction particle (Sigma Aldrich). Control Caco2 clones (shNT ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant xLIG1 was eluted twice in 250 μl EB containing 1 mM DTT and 250 μg/ml 3×FLAG peptide (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... GC B cells were added starting from 3000 cells/well to 1 cell/well through 3-fold dilution steps in the presence of 30 μg/ml of LPS (Salmonella typhimurium, Sigma-Aldrich) and 2 ng/ml of IL-21 (Peprotech) ...
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bioRxiv - Immunology 2021Quote: ... that contain two parallel channels separated by a porous membrane (7 μm pores) were first perfused and incubated with 1% 3-Aminopropyltrimethoxysilane (Sigma, 281778) in ethanol for 1 hour and then incubated in an 80°C oven overnight ...
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bioRxiv - Microbiology 2022Quote: 1 μg of un-labelled purified CVA9 was mixed with 1 - 3 μL of 35S labelled CVA9 (showing approximately 1000 CPM) and faf-BSA (Sigma-Aldrich) or buffer giving final concentrations as indicated in Table 1 was added to obtain final volume of 100 μL ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant xDnmt1 was eluted twice in 250 µL EB containing 1 mM DTT and 250 µg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2022Quote: ... The following secondary antibodies diluted to 1:6000 in 3% milk and applied for two hours at room temperature: goat anti-Rabbit IgG peroxidase (Sigma #A0545) and goat anti-mouse IgG peroxidase (Sigma #A0168) ...
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bioRxiv - Cancer Biology 2022Quote: ... washed 3 × 10 mins in PBS and incubated for 1 hr in PBS containing 10 mg/mL sodium borohydride (Sigma 213462) to quench autofluorescence ...
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bioRxiv - Immunology 2022Quote: ... cells permeabilized with 0.5% TritonX-100 (company) for 1 min and then blocked at room temperature for 30 min in 3% bovine serum albumin (Sigma-Aldrich) buffer ...
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bioRxiv - Physiology 2022Quote: ... Adipocyte differentiation was induced in DMEM with 10% Fatal Bovine serum (FBS) using 500 μM 3-isobutyl-1-methylxanthine (IBMX, Sigma I5879), 5μg/ml insulin (Sigma I9278) ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 μM or 1 mM from a 0.5 M stock solution of 3-MB (Sigma-Aldrich, St Louis, MO, USA) solubilized in 0.5 M NaOH ...
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bioRxiv - Immunology 2024Quote: ... B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol) FBS and 1% penicillin-streptomycin and activated with 3 μg/mL LPS (Sigma-Aldrich), 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma) in washing solution for 1h ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... Proteins were eluted by rotating the resin at room temperature for 1 hour in 100 μL of PBS containing 150 ng/ μL 3×-FLAG peptide (Sigma Aldrich). 100 μL of 2x sample loading buffer was added to the eluate and samples were boiled at 95 °C for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... 100 mM NaCl with 0.1% n-Dodecyl-B-D-Maltoside to remove excess SDS by using an 30 kDa amicon centrifugal filter (1/10 dilution, 3 times; EMD Millipore) before a final overnight dialysis (1/500 dilution ...
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bioRxiv - Bioengineering 2023Quote: A 10 mg/mL suspension of TAG drops was prepared by dissolving 1 mg of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.) in 100 μL of a chloroform–methanol solution (2:1) ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... 15N labelled protein was made from a 1 L culture containing M9 minimal media salts supplemented with 3 g of 13C-glucose (Sigma-Aldrich), 1 g of 15N ammonium chloride (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... MCF10A JUN knockout cells expressing JUN-AID-V5 fusion protein and Transport inhibitor response 1 (TIR1) were grown to 60-70% confluency and treated with 100 µM indole-3-acetic acid (IAA, Sigma-Aldrich) for 1h to deplete JUN protein ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... A 5% (v/v) of washed >30 kDa FBS fraction was premixed with a 1:3 serial dilution of CaCl2 (#C5080, Sigma-Aldrich) diluted in 1X PBS (concentration range from 0.5 to 0 mM ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Cell Biology 2023Quote: ... and puromycin to select transduced cells (1 μg/ml for hNPCs; 3 μg/ml for SH-SY5Y cells; Sigma, Cat# P9620). Cells were passaged 4-5 times after lentiviral transduction to select for stable expression of shRNAs.
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bioRxiv - Cancer Biology 2023Quote: ... IFI44L (#1: TRCN0000167757; #3: TRCN0000242541) or ISG15 (#4: TRCN0000007423)) in the presence of X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich). Five shRNAs per gene were tested for knockdown efficiency ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL, Sigma-Aldrich) before mounted ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...