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Citations for Millipore Sigma :
5001 - 5050 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... washed 3 × 10 mins in PBS and incubated for 1 hr in PBS containing 10 mg/mL sodium borohydride (Sigma 213462) to quench autofluorescence ...
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bioRxiv - Immunology 2022Quote: ... cells permeabilized with 0.5% TritonX-100 (company) for 1 min and then blocked at room temperature for 30 min in 3% bovine serum albumin (Sigma-Aldrich) buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... A 5% (v/v) of washed >30 kDa FBS fraction was premixed with a 1:3 serial dilution of CaCl2 (#C5080, Sigma-Aldrich) diluted in 1X PBS (concentration range from 0.5 to 0 mM ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Cell Biology 2023Quote: ... and puromycin to select transduced cells (1 μg/ml for hNPCs; 3 μg/ml for SH-SY5Y cells; Sigma, Cat# P9620). Cells were passaged 4-5 times after lentiviral transduction to select for stable expression of shRNAs.
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bioRxiv - Cancer Biology 2023Quote: ... IFI44L (#1: TRCN0000167757; #3: TRCN0000242541) or ISG15 (#4: TRCN0000007423)) in the presence of X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich). Five shRNAs per gene were tested for knockdown efficiency ...
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bioRxiv - Immunology 2024Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Microbiology 2024Quote: ... The pepsin was inactivated with 2ml Na2CO3 and 2ml 1% DMEM and the parasites were then filtered through a 3-μm Whatman Nucleopore filter (Millipore Sigma). Bradyzoites were pelleted (5 min at 2000xg ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were co-transfected with NSP6 and mCherry-DFCP1 expression vectors using X-tremeGENE 9 DNA Transfection Reagent at a 1:3 ratio in serum-free Optimem (Millipore Sigma). After 72 hours ...
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... Lipids from bovine brain extract (Folch fraction 1) and 16:0/16:0-PI(3)P were purchased from Sigma Aldrich and Echelon Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HESCS were moved to a hypoxia incubator (3% O2) and treated with a differentiation cocktail containing 1 μM progesterone (Sigma- Aldrich), 10 nM 17-β-estradiol (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 x 10 min in PBS and incubated for 1 h in PBS containing 10 mg/mL sodium borohydride (Sigma, 213462) to quench autofluorescence ...
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bioRxiv - Neuroscience 2024Quote: Flies were kept for at least 3 days prior to the experiment on food containing retinal (1 ml alltrans retinal (Sigma-Aldrich) solution (100 mM in 95% ethanol ...
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bioRxiv - Developmental Biology 2024Quote: ... Tadpoles were then washed 3 × 10 minutes in PBS-Triton followed by a 10 minute incubation in 1:2000 DAPI (Sigma D9542) in PBS-Triton before 3 × 20 minute washes in PBS-Triton ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with 40 µM BrdU/BrdC (ratio 3:1, BrdU: B5002, Sigma-Aldrich, BrdC: sc-284555, Santa Cruz Biotech) for 18-24 hours prior to fixation (see cell synchronization).
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with HBSS buffer containing 3% BSA for 20 min followed by incubation with monoclonal anti-HA-FITC antibody (1:500) (Sigma, H7411) for 1 h at RT in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293T cells were transfected with either PSKH1-3C-FLAG or pCDNA3 EGFP as a control using a 3:1 polyethylenimine (PEI [branched average Mw ∼25 000 Da; Sigma–Aldrich]) to DNA ratio (30 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3–5 μM of the mCherry-fusion protein was incubated with 1 mg/mL bovine serum albumin (BSA) (Sigma-Aldrich, A3059) in 1×TBS-Ca2+ for 15 minutes at room temperature (Supplementary Fig ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells were transfected with 1 µg/ml plasmid DNA complexed with 3 µg/ml branched Polyethylenimine (PEI) (Sigma-Aldrich # 408727). After 18 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Tadpoles were then washed 3 × 10 min in PBT followed by a 10-min incubation in 1:2000 DAPI (Sigma D9542) before being washed with 1xPBS for 10 more minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Fully grown GV oocytes were isolated into transfer medium (TM) supplemented with 100 μM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich) to prevent spontaneous meiotic resumption ...
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bioRxiv - Bioengineering 2020Quote: ... or covalently crosslinked with 75 mM of dihydrazide (AAD)/1-hydroxybenzotriazole (HOBT) with 100 mg/mL of 1-ethyl-3- (dimethylaminopropyl) carbodiimide (EDC) (all from Sigma-Aldrich, St. Louis, MO) (Table 1) ...
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bioRxiv - Physiology 2024Quote: ... for 10 days and treated with 100 nM endothelin-1 (ET1) or vehicle (H2O) the last 3 days as previously described.25 Cardiomyocytes were then stained for α-actinin (Sigma, SAB2108642, 1:800) and N-cadherin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatants were transferred to cluster tubes (Axygen, PN MTS-11-12-C) and treated with 24 µL of 1:1 solution of Benzonase (Millipore Sigma, PN 70746-3) and 100 mM MgSO4 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were diluted in a 5:1 (5X) stimulation buffer containing 500 µM of 3-isobutyl-1-methylxanthine (IBMX) (Sigma Aldrich, Saint Louis, MO). To an HTRF 96-well low volume white plate (CisBio) ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Lipids were extracted from the cell pellets through one-phase extraction with 202 uL of BuOH:MeOH (1:1) (Sigma 71-36-3 and 67-56-1) that contains 10 umol of cholesterol-d7 (Cayman Chemical 83199-47-7 ...
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bioRxiv - Physiology 2024Quote: Membranes were incubated with primary antibodies in 5% milk (α-Mitofusin1, rabbit, Proteintech, #13798-1-AP, 1:500; α-Calreticulin, Thermo Fisher, #PA 3-900, 1:1000; α-β-tubulin, mouse, Sigma Aldrich #T4026 ...
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bioRxiv - Molecular Biology 2021Quote: Mouse liver samples for Myd88−/− and TLR4−/−: Mice of approximately 3-months were injected intraperitoneally with LPS (Escherichia coli O111:B4, Sigma, 2 μg/gm body weight) or PBS for 18 h [110] ...
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bioRxiv - Microbiology 2020Quote: ... whereas influenza B virus was amplified at 35°C for 3 days in MDCK cells in the presence of 2 μg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA). SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020) ...
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bioRxiv - Biophysics 2022Quote: ... silanization of another set of glass coverslips were performed by plasma activation of clean coverslips and then incubated with an ethanol solution containing 2% (v/v) 3-(trimethoxysilyl) propyl methacrylate (Sigma-Aldrich, St Louis, Missouri, USA) and 1% (v/v ...
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bioRxiv - Biophysics 2021Quote: ... coverslips were then modified to present surface amines by mounting them in a coplin jar and submerging them in a 2% v/v solution of (3-aminopropyl) triethoxysilane (APTES) (Sigma-Aldrich, catalog no. A3648-100ML) in acetone for 10 min ...
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bioRxiv - Pathology 2023Quote: ... To prevent unspecific labelling the sections were incubated for 2 × 10 minutes in PB solution containing 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS, Oslo, Norway), and then incubated for 1 hour in PB solution containing 5% normal goat serum (Abcam Cat# ab7481 ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS, Oslo, Norway), and next incubated for 1 hour in a solution of PB with 5% normal goat serum (Abcam Cat# ab7481 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were anesthetized and the regenerating part was dissected and collected into 0.5 ml of 2/3 PBS (Sigma D8537, diluted by RNase-free water) with Actinomycin D (ActD ...
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bioRxiv - Microbiology 2024Quote: ... 2 ug mL-1 N-(6-(isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine hydrochloride (GSK’872) (Sigma-Aldrich # 5303890001) and 2 ug mL-1 Necrosulfanamide (EMD Millipore #480073 ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were incubated with primary antibodies in 3%BSA for 1 hr at room temperature: monoclonal anti-SMN (05-1532 Sigma-Aldrich clone 2B1, 1:1000); rabbit anti- Coilin (H300 Santa Cruz SC-32860 ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies employed in this experiment were as follows: mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, 1:4000), anti-Flag (F3165, 1:2000) from Sigma-Aldrich (St. Louis, MO, USA); mouse anti-hemagglutinin (HA ...
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bioRxiv - Microbiology 2022Quote: ... each flask was spiked with 3 ml of 100 mg NH4+-N L-1 NH4Cl or 100 mg Mn2+ L-1 MnCl2·4H2O (Sigma Aldrich, Saint Louis, Missouri USA). Liquid samples (1 ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo, Millipore, #WBLUR0100) for chemiluminescence detection using ChemiDoc™ MP Imaging System (Bio-Rad).
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... and the Arp2/3 inhibitors CK-548 and CK-636 were from Sigma (St. Louis, MO). IL-4 was from Genscript (Piscataway ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to stagetips containing 3×14G plugs of 3MTMEmporeTM SDB-RPS substrate (Sigma) as described previously (Kulak et al. ...
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bioRxiv - Cell Biology 2020Quote: ... washed 3 × 5 mins in phosphate-buffered saline (PBS) with 0.1% Tween-20 (both Sigma-Aldrich) and probed for 1 hour at room temperature in the dark with IRDye® 800CW conjugated goat anti-mouse secondary antibodies (LiCOR Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed in PBS and incubated in 3% bovine serum albumin (BSA) (Sigma-Aldrich) diluted in PBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... shLmnA (produced in our lab), shLMNC (produced in our lab, 5′-TCTCCCACCTCCATGCCAAAG-3’) or shLMNB1 (Sigma clone NM_010721.1-956s1c1 ...
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bioRxiv - Cell Biology 2020Quote: ... washed and developed with chromogenic 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, Sigma-Aldrich, B6149).
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bioRxiv - Cell Biology 2020Quote: ... organoids were rinsed twice with warm PBS and incubated with warm PBS containing 3% formaldehyde (Sigma) for 20 minutes ...