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Citations for Addgene :
551 - 600 of 1322 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments of CgB (DNASU) and CLIP (gift from Eric Campeau; RRID:Addgene_29650) were subcloned into pENTR-RIP via Gibson assembly ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847) and an NheI/PmeI-digested piggyBac dox inducible expression vector PB-TRE-Cas91 including a puromycin selection marker ...
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bioRxiv - Synthetic Biology 2022Quote: ... pCasTn plasmid cloning: ShCAST genes were PCR amplified from pHelper_ShCAST_sgRNA (Addgene #127921). The vector backbones were amplified from plasmids carrying an RK2 origin of replication with a Kanamycin resistance gene for pKSh2 (Addgene #167515 ...
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bioRxiv - Neuroscience 2020Quote: ... dLight1.1 sequence was PCR amplified from pCMV.dLight1.1 (Addgene #111053, Dr. Lin Tian)56 using Q5 polymerase (primers ...
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bioRxiv - Biochemistry 2022Quote: ... mCherry was PCR amplified from mCherry-Alpha-5-Integrin-12 (Addgene #54970) using forward primer 54970RMmCherryaddNHis_F2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR with pKD3 (pKD3 was a gift from Barry L. Wanner; Addgene plasmid #45604 ...
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bioRxiv - Genetics 2020Quote: ... The His2Av polyA sequence was PCR amplified from pDEST-APLO (Addgene 112805). DNA fragments were assembled together using NEBuilder DNA Assembly (New England Biolabs Inc.).
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bioRxiv - Neuroscience 2020Quote: ... secA5 sequences were amplified by PCR from pBH-UAS-secA5-YFP (Addgene) and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene) sequences ...
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bioRxiv - Biochemistry 2020Quote: ... The EMCV IRES sequence was amplified by PCR from EMCV_IRES_pcDNA4TO_H2B_SunTag24x_v1 (Addgene #246719) using the following primers ...
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bioRxiv - Genomics 2021Quote: ... The PCR amplicon was cloned into a lacZ reporter vector (Addgene #139098) using Gibson assembly (New England Biolabs)57 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MetRSL274G coding sequence was PCR amplified using pMarsL274G construct (Addgene 63177) as a template and inserted into BamHI and MluI sites within the pLV-EF1a-IRES-puro vector (Addgene 85132 ...
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bioRxiv - Cancer Biology 2021Quote: ... TUBA1B was amplified using PCR (donor plasmid: Addgene #57159, from Michael Davidson) and an EGFP-TUBA1B fusion protein was generated with two-stage overhang extension PCR using the TUBA1B and EGFP cDNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Cell Biology 2022Quote: ... 24xSuntag (24xGCN4) was PCR-amplified from pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (Addgene, #74928) and inserted between the BsrGI and ApaI sites of pcDNA-MCP-mCherry.
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bioRxiv - Molecular Biology 2022Quote: ... IκBα DNA was PCR amplified from pBABE-GFP-IκBα-wt (Addgene, 15263) and was digested and cloned into pET28-CBP-His6 ...
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bioRxiv - Neuroscience 2022Quote: ... we first PCR amplified myr- 4xSNAPf from pUAS-myr-4xSNAPf (Addgene #87637) using FRT-containing primers ...
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bioRxiv - Genomics 2022Quote: ... KRAB(ZIM3) was PCR-amplified from pLX303-ZIM3-KRAB-dCas9 (Addgene # 154472). The final construct is KRAB(ZIM3)-dCas9 or Zim3-dCas9 for short ...
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with AgeI and XbaI and cloned into cut sites upstream of HaloTag insert in pGW1-Halo.
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with KpnI and SalI and cloned into cut sites upstream of the 2A in the pGW1-2A-GFP vector.
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bioRxiv - Cancer Biology 2023Quote: ... The IRES site was amplified by PCR from pInducer-21 (Addgene #46948). YAP5SA was amplified by PCR from Addgene #33093 ...
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bioRxiv - Immunology 2023Quote: ... eGFP was amplified via PCR from pLJM-1-eGFP (Addgene: Plasmid #19319) using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1 ...
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bioRxiv - Pathology 2023Quote: Cre recombinase DNA was amplified by PCR using pCAG-Cre (Addgene #13775) as a template ...
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bioRxiv - Cancer Biology 2023Quote: ... The bGH poly(A) signal was PCR amplified from pX459 (Addgene, 62988) using primers forward ...
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bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR amplicon was cloned into a lacZ-reporter vector (Addgene #139098) or mCherry reporter vector (available upon request ...
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bioRxiv - Microbiology 2023Quote: ... mKO2 was PCR amplified from the template mKO2-N1 (Addgene, Cat. #4625) using primers containing matching restriction sites ...
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bioRxiv - Neuroscience 2023Quote: ... and a synthetic intron was PCR-amplified from pDEST-APLO (Addgene 112805) (77) ...
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bioRxiv - Cancer Biology 2023Quote: ... were ordered from GenScript (https://www.genscript.com) and PCR-cloned into pHIV-Luc-ZsGreen (Addgene plasmid #39196) using and EcoRI and XbaI digestion.
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bioRxiv - Neuroscience 2023Quote: ... a Cas9-T2A fragment PCR amplified from pDEST-APIC-Cas9 (Addgene 121657) (9) ...
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bioRxiv - Systems Biology 2024Quote: ... CYC1t and pTEF1 were both PCR amplified from BB3cK_pGAP_23*_pTEF_Cas9 (Addgene #104909) (Gassler et al. ...
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bioRxiv - Cell Biology 2023Quote: ... by PCR amplifying the ORFs from cDNA templates of HSC70 (19514, Addgene) and LAMP2A (86146 ...
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bioRxiv - Cell Biology 2023Quote: ... with HA-TurboID amplified by PCR from 3xHA-TurboID-NLS_pCDNA3 (Addgene #107171) and inserted with a Gly-Gly-Gly-Ser linker either side of the HA-TurboID sequence ...
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bioRxiv - Systems Biology 2022Quote: ... and lambda phosphatase were purchased from Addgene (Addgene Kit #1000000094)7.
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bioRxiv - Cancer Biology 2021Quote: ... CyclinB1-GFP was amplified using PCR (donor plasmid: Addgene #26061, from Jonathon Pines) and was subcloned into pDONR221 and subsequently subcloned into pLX303 ...
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bioRxiv - Molecular Biology 2020Quote: ... ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425 ...
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bioRxiv - Genetics 2021Quote: ... The sequence of GeneSwitch was amplified by PCR using pElav-GeneSwitch (Addgene: 83957). The backbone vector pElav-GeneSwitch was digested with KpnI ...
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bioRxiv - Developmental Biology 2020Quote: MoxMaple3 was amplified by PCR from the pmCherry-T2A-moxMaple3 (Addgene Plasmid #120875) [43] and inserted into the pUASp by SpeI (3’)/EcoRI (3’) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplicons of sT and LT gene regions from plasmid RAZ1 (Addgene #114381) were used as target DNA templates (Refer Supplementary Table 1 for primers) ...
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bioRxiv - Genomics 2020Quote: ... PCR amplified and cloned into Esp3I-digested plentiGuide-Puro (Addgene plasmid ID: 52963) lentiviral vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... the natNT2 marker was PCR amplified from pUG-natNT2 (Addgene plasmid #110922, (26)) using primers 10297 and 10298 ...
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bioRxiv - Neuroscience 2019Quote: ... the iGluu gene was amplified by PCR from pCI-syn-iGluu (Addgene #106122) using Phusion polymerase (forward 5’-CATCAGGATCCATGGAGACAGACACACTCC-3’ ...
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...
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bioRxiv - Bioengineering 2021Quote: ... Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966) as a template and cloned together with either pJ23119 or pAtU6 for sgRNA expression.
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bioRxiv - Cell Biology 2021Quote: The KIF18B coding sequence was amplified by PCR from 24xMoonTag-kif18b-24xPP7 (Addgene plasmid #128604 [38] was a gift from Marvin Tanenbaum ...
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bioRxiv - Neuroscience 2019Quote: ... nGFP was PCR amplified from pAd nGFP (Addgene, 19413, deposited by Douglas Melton) using primers (forward 5’-CATCATCGAT ATGGTGCACGTGGATCCAA −3’ ...
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bioRxiv - Biophysics 2022Quote: ... Kinesin 1 1-401 (K401) was PCR amplified from pWC2 plasmid (Addgene # 15960). Ncd 236-701 was PCR amplified from a plasmid gifted by Andrea Serra-Marques.
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bioRxiv - Microbiology 2020Quote: The SNAP-tag gene was PCR amplified from pSNAP-tag(m) (Addgene #101135) and cloned into pVpr-IN.eGFP(Albanese et al. ...
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bioRxiv - Microbiology 2021Quote: ... The Cas9 part was generated via PCR amplification of pCas9/Decoy (Addgene #80324)8 using primers P12/P13 ...