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Citations for Addgene :
451 - 500 of 957 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel-purified and cloned into EcoRV-cut pLenti CMV Puro DEST (w118-1) (Addgene #17452 ...
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bioRxiv - Cell Biology 2021Quote: ... The miRFP670 was amplified by PCR from pmiRFP670-N1 (a gift from Vladislav Verkhusha, Addgene plasmid #79987) (Shcherbakova et al. ...
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bioRxiv - Genetics 2022Quote: ... as the selection module for all TFT constructs and obtained this sequence by PCR amplification from Addgene plasmid #41030 (a gift from Michael Boddy) ...
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bioRxiv - Cancer Biology 2022Quote: ... After PCR the insert was gel purified (GeneJet) and Gibson cloned into BsmBI-digested pXPR_053 (Addgene# 113591). Gibson cloned insert and vector was column purified (GeneJet ...
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bioRxiv - Bioengineering 2022Quote: ... another fragment coding sfGFP was PCR-amplified from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (#60904, Addgene) using oKK334 and oKK335 ...
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bioRxiv - Developmental Biology 2022Quote: ... EF1alpha-GCamp6m lentiviral vector was generated by PCR amplification of GCamp6m from pGP-CMV-GCaMP6m (Addgene #40754) using with Q5 High Fidelity master mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... the doxycycline inducible Tet-On-3G_hPGK promoter sequence was PCR amplified from the plasmid pMK243 (Addgene, #72835) with 5’ SalI and 3’ MluI digestion sites ...
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bioRxiv - Cell Biology 2022Quote: ... The BG4 sequence was amplified by PCR from the pSANG10-3F-BG4 plasmid (Addgene plasmid # 55756(12)) while the mitochondrial targeting sequence (MTS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We PCR amplified LexApr using oligos 1195 and 1240 from FRP1642 (Addgene #58442, Ottoz et al. 2014). We PCR amplified CYC1 terminator from pSZB1040 using oligos 2194 and 2195 ...
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bioRxiv - Microbiology 2019Quote: ... NSP5 and NSP2-mCherry were amplified by PCR and inserted into the plasmid pAIP (Addgene #74171; (30) at the NotI-EcoRI restriction enzymes sites to yield pAIP-NSP5 and pAIP-NSP2-mCherry ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared by PCR and transferred to a retroviral plasmid backbone (a gift from Fred Gage (Addgene plasmid # 49054 ...
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bioRxiv - Neuroscience 2021Quote: ... The low affinity glutamate sensor iGluSnFR S72A was PCR amplified from the pAAV.hSynapsin.SF-iGluSnFR.S72A which was a gift from Loren Looger (Addgene plasmid # 106176 ...
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bioRxiv - Developmental Biology 2020Quote: 1 kb homology arms were generated through PCR and cloned into the pHD-dsRed-attP (Addgene #51019). Guide RNAs and the donor vector were co-injected into vas-Cas9 embryos (BDSC #51324 ...
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bioRxiv - Microbiology 2019Quote: ... we PCR-amplified the G418 resistance cassette from the plasmid pFA6a-6xGLY-Myc-kanMX6 (Addgene, plasmid #:20769), flanked by 50-bp upstream and downstream regions of the start and stop codons of the corresponding heme biosynthetic gene ...
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bioRxiv - Molecular Biology 2021Quote: ... 2500 ng of purified HDR PCR product and 1500 ng of guide RNA expression plasmid (Addgene 49330) containing the relevant guide RNAs (Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2022Quote: ... was used for PCR reactions with 30-50 ng of pScarlessHD-DsRed plasmid as template (Addgene #64703) or 50 ng of gBlocks or genomic DNA from the same Drosophila strain (BDSC #55821) ...
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bioRxiv - Biochemistry 2022Quote: ... Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386 ...
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bioRxiv - Genetics 2022Quote: ... 1 kb homology arms were generated through PCR and cloned into the pHD-dsRed-attP (Addgene #51019) [73] ...
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bioRxiv - Synthetic Biology 2022Quote: ... It was synthesised by PCR amplifying the αGCN4 backbone from LLP252 pEF1a-NLS-scFvGCN4-DNMT3a (Addgene, #100941) and the first 639 bp of the 5’ end of p65HSF1 from pAC1393-pmax-NLSPUFa_p65HSF1 (Addgene ...
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bioRxiv - Synthetic Biology 2022Quote: ... and CAS9 was inserted via Gibson Assembly after PCR via Cr_1250 and Cr_1254 from pCRISPRyl (Addgene #70007). Integration was accomplished as previously described using a two plasmid CRISPR-mediated markerless approach 44 ...
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bioRxiv - Microbiology 2022Quote: ... PTEN coding sequence was PCR amplified from cDNA and cloned into pLV-EF1a-IRES-Neo (Addgene #85139) (80 ...
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bioRxiv - Plant Biology 2023Quote: ... NJ) or amplified by PCR with overhangs containing SapI recognition sites and assembled into pUAP4 (Addgene #136079) to create parts compatible with the Phytobrick standard (74) ...
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bioRxiv - Neuroscience 2023Quote: The Pink Flamindo cDNA was amplified by PCR from the plasmid (provided by Kitaguchi, Addgene plasmid #102356). The amplified fragment was then integrated into the 20xUAS vector originating from pJFRC7-20xUAS-IVS-mCD8::GFP (Pfeiffer ...
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bioRxiv - Molecular Biology 2023Quote: ... template libraries were constructed by PCR sewing and cloned into pRSII413 (a gift from Steven Haase, Addgene plasmid #35450 ...
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bioRxiv - Microbiology 2023Quote: ... the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed ...
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bioRxiv - Cell Biology 2023Quote: ... the AGO2 CDS was PCR-amplified using pFRT/FLAG/HA-DEST EIF2C2 plasmid (Addgene: Cat. # 19888[57]) as template and Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... we generated PCR amplified EF1a promoter and TetR-NLS signal cassette from pLV-tTRKRAB-red (Addgene, #12250). 3xFlag-KRAB was PCR amplified from the same vector with a forward primer including the sequence encoding 3xFlag and SV40pA amplified from lentiCRIPSRv2 ...
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bioRxiv - Genetics 2023Quote: ... the T7 promoter sequence was added to the sgRNA template by PCR amplification using pX335 (Addgene #42335) with Phusion high-fidelity DNA Polymerase Kit (NEB) ...
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bioRxiv - Genetics 2023Quote: To replace nvYFP with mCherry we PCR amplified mCherry from pQC NLS mCherry IX (Addgene plasmid 37354) using primers #1833 and #1834 and PCR amplified attB-Dfd-3XP3 from pAttB-Dfd-3XP3-nvYfp with primers #1831 and #1832 and performed Gibson assembly to generate plasmid #795 = pAttB-Dfd-3xP3-mCherry.
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bioRxiv - Developmental Biology 2023Quote: ... we added the T7 promoter sequence to the sgRNA template by PCR amplification of px330 (Addgene, 42230) using the primer pair listed in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... human RXRa cDNA was PCR-amplified from pSV-Sport-RXRα (a gift from Bruce Spiegelman; Addgene #8882)68 ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR cassette was transfected into HeLa cells with a helper plasmid containing AsCas12a endonuclease (Addgene 89353). Following cleavage ...
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bioRxiv - Developmental Biology 2023Quote: PCR amplification of the repair template was performed using 50 ng of plasmid containing mNeonGreen (Addgene 125134) as template in a touchdown PCR reaction (annealing temperature decreased 1°C from 65°C to 50°C for the first 15 cycles followed by 20 cycles with an annealing temperature of 50°C ...
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bioRxiv - Genetics 2024Quote: ... the T7 promoter sequence was added to the sgRNA template by PCR amplification using pX335 (Addgene #42335) with Phusion high-fidelity DNA Polymerase Kit (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... from Sylvestre Marillonnet (Addgene kit # 1000000044). Specific parts (target gRNA or siRNA sequence ...
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bioRxiv - Synthetic Biology 2022Quote: The MoClo Toolkit (Addgene Kit #1000000044), MoClo Plant Parts Kit (Addgene Kit #1000000047) ...
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bioRxiv - Synthetic Biology 2022Quote: ... GreenGate Cloning System (Addgene Kit #1000000036) and amilCP_Orange chromoprotein vector (Addgene Plasmid #117850 ...
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bioRxiv - Genetics 2023Quote: ... and pX330S-2 (Addgene Kit 1000000055) according to the kit instructions ...
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bioRxiv - Bioengineering 2024Quote: ... including existing parts (Addgene Kit # 1000000080) and custom-made parts (Table 2) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA sequences between nucleotides 1-3877 and 4236-4617 were PCR amplified from V5-GFP-P180 (Addgene #92150) and the two fragments were assembled and cloned into pEGFP(A206K)-N1 between XhoI and BamHI sites by GIBSON assembly ...
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bioRxiv - Genetics 2021Quote: TPM2 mammalian expression constructs were generated by PCR subcloning variants from pUASt.attB into pCMV-IRES-eGFP (Addgene 78264) using XbaI/EcoR1 ...
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bioRxiv - Neuroscience 2021Quote: hM4D(Gi) was amplified by PCR from pcDNA5/FRT-HA-hM4D(Gi) (Addgene #45548 (Armbruster et al., 2007)) using forward primer ...
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bioRxiv - Neuroscience 2021Quote: Kir was amplified by PCR from pCAG-Myc-Kir2.1E224G/Y242F-2A-EGFP (similar to pCAG-Kir2.1-T2A-tdTomato, Addgene #60598 ...
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bioRxiv - Genetics 2020Quote: ... A His2Av polyA sequence after the BFP/Gal80 coding sequence was PCR amplified from pDEST-HemmarR2 (Addgene # 112813).
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bioRxiv - Molecular Biology 2019Quote: Primers used for PCR amplification of vector inserts from existing plasmids (pL451, tdTomato, AAV from Addgene plasmid# 60229).
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213925 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were then subcloned into the pDEST-ORF-v1 (a kind gift from Darren Saunders, Addgene, #73637) (Croucher et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs, Addgene # 25999) (Beronja et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ; http://n2t.net/addgene:122848; RRID:Addgene_122848) (94 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA sequences of full-length CAD and its fragments were amplified by PCR from pcDNA3.1-HisFlag-CAD (Addgene) and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences ...