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Citations for Addgene :
651 - 700 of 957 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... an hM3Dq-mCherry cassette was PCR-amplified using pAAV-hSyn-DIO-hM3Dq-mCherry (Addgene #44361, donated by Dr. Bryan Roth) as a template with primers containing 5’-MluI site and 3’-EcoRI site and then inserted into pENTR1A-TRE between the MluI/EcoRI sites ...
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bioRxiv - Plant Biology 2023Quote: ... BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from an gRNA scaffold template (AddGene no ...
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bioRxiv - Genomics 2022Quote: The PCR product was cloned into the pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5 vector (Addgene #6125554) using HindIII and XbaI restriction sites ...
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bioRxiv - Neuroscience 2022Quote: Full length human TAOK1 was obtained from Transomics Technologies in PCR-XL-Topo plasmid and inserted into sfGFP-C1 (Plasmid #54579, Addgene), using the EcoRI and MfeI sites ...
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bioRxiv - Molecular Biology 2022Quote: ... R179A and R320A were PCR amplified and cloned into pET Strep II co-transformation cloning vector (13S-R, Addgene # 48328). Csb3/I-G was cloned into pQE2 (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs encoding cyclin D1 WT and cyclin D1 (T286A) were PCR amplified from pcDNA cyclinD1 HA and pcDNA cyclinD1 HA T286A (Addgene plasmids #11181 and # 11182 ...
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bioRxiv - Microbiology 2022Quote: UAS-SARS-CoV-2-ORF3a transgenic flies were generated by PCR-mediated subcloning of the SARS-CoV-2-ORF3a coding sequence (pDONR207-SARS-CoV-2 ORF3a, #141271, Addgene) into pUASt-Attb (EcoRI/XbaI) ...
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bioRxiv - Microbiology 2023Quote: The construct pFUS ΔIDR-YFP was created by PCR amplifying the fus gene from pGST- TEV-FUS (a kind gift from Dr. Aaron Gitler; Addgene plasmid # 29629 ...
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bioRxiv - Neuroscience 2023Quote: ... sfGFP genes were amplified by PCR from pJT119b plasmid which was kindly gifted by by Jeffrey Tabor (Addgene plasmid #50551), and cloned into pETcon-alpha syn ...
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bioRxiv - Cell Biology 2023Quote: ... Obtained pcDNA-Halo was further digested with HindIII/Bam-HI restriction enzymes and ligated with the NPM insert (obtained by PCR amplification from eGFP-NPM (17578 Addgene) with our primers 5’-CCCAAGCTTCCACCATGGAAGATTCGATGGACATGG-3’ and 5’-CGGGATCCAAGAGACTTCCTCCACTGCC-3’ ...
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bioRxiv - Bioengineering 2023Quote: ... Human codon optimized DisCas7-11 protein sequence and the mature DR guide scaffold with golden gate sites were PCR amplified from Addgene plasmids # 172507 and #172508 ...
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bioRxiv - Cancer Biology 2023Quote: ... A sequence containing the 2.4 kbp p21 promoter region was PCR amplified from the pGL2-p21 promoter-Luc (Addgene #33021). The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a-mCherry-3X-NLS (Addgene #125262) ...
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bioRxiv - Genomics 2023Quote: ... the DNA sequence of KRAB repressor domain was amplified by PCR from the pHAGE TRE dCas9-KRAB (Addgene plasmid #50917) and cloned in frame into the PB-TRE-dCas9-VPR backbone (Addgene plasmid #63800 ...
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bioRxiv - Neuroscience 2023Quote: First CaMPARI coding sequence was PCR amplified from plasmid pcDNA3-CaMPARI (gift from Loren Looger & Eric Schreiter, Addgene plasmid #60421) (Fosque et al. ...
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bioRxiv - Genetics 2023Quote: ... a PCR fragment was made by amplifying either HphMX (rad1Δ) or NatMX (rad51Δ and pol32Δ) from pAG32 (Addgene plasmid # 35122) or pAG25 (Addgene plasmid # 35121) ...
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bioRxiv - Developmental Biology 2023Quote: ... Shox2 or TAk1 were amplified by PCR and directionally cloned downstream of the TurboRFP reporter using ClaI and MluI restriction sites in the pTripZ vector (Addgene) viral constructs were then validated by sequencing ...
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bioRxiv - Microbiology 2023Quote: ... A fragment of MeCP2 containing the T158M mutation was amplified by PCR and cloned in pDEST-hMeCP2-GFP (a gift from Huda Zoghbi, Addgene plasmid # 48078 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the lentivector backbone was Gibson-assembled with a miniU6-tracrRNA gene block and U1a-SpyCas9 amplified by PCR from Addgene plasmid #121507 ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro target specific gRNA cleavage activity was validated by transfecting N2A cells with PCR amplified gRNA gblock and Cas9 plasmid DNA (px330, Addgene) using ROCHE Xtremegene HP ...
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bioRxiv - Cell Biology 2022Quote: ... MLKL gene and its truncated version MLKL(1-182) were amplified by standard PCR from pRetrX-TRE3G-hMLKL-Venus (Addgene_106078) using primers MLKL_Fw and MLKL_Rv for the first ...
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bioRxiv - Molecular Biology 2023Quote: ... The Nexn coding sequence was PCR amplified from the cDNA of murine hearts and subcloned into pFLAG-MRTFA (Addgene #11978) to create the pFLAG-Nexn plasmid.
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding Ifi205 ORF and Ifi205K364ER299E ORF were amplified by PCR then subcloned between the BamHI and EcoRI restriction sites of pGEX-6P-1(Addgene). Ifi205 proteins were expressed as GST-Ifi205-FLAG-fusions in the E ...
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bioRxiv - Bioengineering 2023Quote: ... the coding sequence was amplified by PCR from pVITRO1-Trastuzumab-IgG1/κ which was a gift from Andrew Beavil (Addgene plasmid # 61883 ...
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bioRxiv - Cell Biology 2023Quote: ... Olivier Pertz (University of Bern, Switzerland)44 was PCR-amplified and inserted into pLV-EF1a-MCS-IRES-Blast (Addgene 85133) using Gibson assembly88 ...
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bioRxiv - Bioengineering 2023Quote: ... the 13.5-kb SphI-digested and gel-extracted eDA83 was ligated with a 980-bp PCR-amplified KanR (using oligos 321/322 and vector pUC57-Kan (Addgene) as a template) ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence for MZT2A was PCR amplified from pACEBac1-ZZ-TEV-MZT2-3C-mEGFP (a gift from Tarun Kapoor (Addgene plasmid # 178074 ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence for amino acids 1-110 of GCP2 were PCR amplified from pACEBac1-gamma-TuSC (a gift from Tarun Kapoor (Addgene plasmid # 178079 ...
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bioRxiv - Cancer Biology 2023Quote: Human AKT1_D274A was generated by overlapping PCR mutagenesis using wild-type AKT1 (a gift from Thomas Leonard, Addgene plasmid 8656133) and cloned into a pAceBac1 vector with N-terminal His10-StrepII-(tev ...
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bioRxiv - Cell Biology 2023Quote: ... the ORF was amplified by PCR using Halo-SMARCA5 (#FHC01216; Kazusa) as a template and inserted into sfGFP-N1 vector (#54737; Addgene) digested with EcoRI using the In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2023Quote: pVHL-stabilized HIF-2α (P405A/P531A) was amplified by PCR from the HA-HIF2alpha-P405A/P531A-pcDNA3 plasmid (Addgene, 18956) using primers BamHI-HIF2Af and AscI-HIF2Ar using NEB Q5 High-Fidelity 2X Master Mix according to the instructions of the manufacturer ...
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bioRxiv - Genetics 2023Quote: The denAsCas12a open reading frame was PCR amplified from pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776) (Addgene plasmid # 107943 ...
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bioRxiv - Microbiology 2024Quote: ... 782 bp PCR product obtained with primers ymCherryU and ymCherryL and pAG426-GAL-ccdb-ymCherry (a gift from Susan Lindquist (Addgene plasmid # 14155 ...
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bioRxiv - Cell Biology 2024Quote: The coding sequence of APEX2 was PCR-amplified from APEX2 plasmid (a gift from Alice Ting, Addgene plasmid #72480, [86]) using specific primers containing restriction sites NotI (5’ ...
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bioRxiv - Molecular Biology 2024Quote: ... the FRT-STOP-FRT cassette sequence was PCR amplified from pJFRC201-10xUAS-FRT-STOP-FRT-myr::smGFP-HA (#63166; Addgene). The coding sequence of mCD8::DQVD/A was PCR-amplified from the genomic DNA of Caspase-Sensitive/Insensitive-Gal4 [20] ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding sequence of mNeonGreen was PCR-amplified from pAc5-V5::mNeonGreen [17] and ligated into the EcoRI-digested pQUAST (#24349, Addgene) vector using In-Fusion ...
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bioRxiv - Genetics 2024Quote: ... pLenti-CMV-HA-HHIP lentiviral expression plasmid was generated by subcloning the appropriate human HHIP cDNA PCR fragment into pLenti-CMV Blast (659–1) (Addgene). HA-tag was inserted into human HHIP cDNA after Ala 23 ...
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bioRxiv - Cell Biology 2023Quote: ... The TRUPATH kit was purchased from Addgene (#1000000163) and was a gift from Bryan Roth ...
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bioRxiv - Synthetic Biology 2023Quote: ... a gift from John Dueber (Addgene kit # 1000000061). The constructs encoding sequential incorporation of serine in place of tyrosine in the FUS IDR was based on previously published sequences66 ...
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bioRxiv - Neuroscience 2020Quote: ... The plasmid kit used for the generation of TALENs was a gift of Daniel Voytas and Adam Bogdanove (Addgene kit #1000000016). Plasmid encoding assembled TALENs was linearized with SacI ...
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bioRxiv - Plant Biology 2020Quote: ... GFP tagged OsHIPP19 and OsHIPP20 were generated by Golden Gate methods (Engler et al. 2008) using MoClo Plant Parts Kit and MoClo Plant Tool Kit (Addgene, UK). OsHIPP19 and OsHIPP20 were cloned into pICH47732 with a GFP tag at the N-terminus ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned the dgn-1 promoter and AcNPV p10 3’UTR into the vector pPD49.26 (from the Andrew Fire plasmid kit, Addgene Kit #1000000001) to generate plasmid pNTC2 ...
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bioRxiv - Cell Biology 2023Quote: ... were constructed in according to Golden Gate TALEN assembly protocol and using the Golden Gate TALEN and TAL Effector Kit 2.0 (Addgene, Kit #1000000024). CIscript-GoldyTALEN was a gift from Daniel Carlson & Stephen Ekker (Addgene ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid construction was performed by modular cloning using the MoClo Tool Kit and the MoClo Plant Parts Kit (Addgene, 28–30). A detailed MoClo protocol and the list of vectors used can be found in 18.
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bioRxiv - Biochemistry 2024Quote: Plasmids encoding GPCRs were obtained from various sources (as indicated Key Resources Table) or subcloned into pcDNA3.1 from the PRESTO-Tango GPCR kit (Addgene Kit #1000000068) as described next ...
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bioRxiv - Plant Biology 2019Quote: ... K353E, and D450N), RBOHD/C (full-length, C1, C2, and C3) were amplified by PCR and cloned into pOPINK (Addgene, #41143) or pOPINM (Addgene ...
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bioRxiv - Molecular Biology 2020Quote: ... A C-terminal Strep tag on ORF24-NTD was added by inverse PCR to generate p6H-SUMO3-ORF24-NTD-Strep (Addgene #138467).
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bioRxiv - Cell Biology 2020Quote: For derivation of HA-Smo and Smo-HA ESCs mouse Smo was amplified by PCR from the pGEN-mSmo (Addgene, #37673) and introduced in the PB-HA-IRES-Neo vector ...
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bioRxiv - Cell Biology 2020Quote: ... To generate PCP-NLS-2xmCherry, we PCR amplified PCP from phage UbiC NLS HA stdPCP st-dGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-ATACTCGAGCGCCAC-CATGGGCCCAAA and 5’-CTCATCGATGGTGGC-GACCGGTGGAC ...
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bioRxiv - Cell Biology 2020Quote: ... To generate MCP-NLS-2xGFP, we PCR amplified 2xEGFP from phage UbiC NLS HA stdPCP stdGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-TCTATCGATATGGTGAGCAAGGG-CGAG and 5’-ATAGCGGCCGCTTATTTGTACAAT-TCATCCATACCATGGG ...
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bioRxiv - Cell Biology 2020Quote: ... To generate the modified TREAT reporter constructs, we first PCR amplified humanized Renilla luciferase from the original TREAT plasmid (Horvathova et al., 2017) (Addgene #104096) using primer sequences 5’-ATACTCGAGCGACTCACTATAGGCTAGCCAC and 5’-ATAGGCCGGCCTTACTGCTCGTTCTTCAG-CAC ...