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Citations for Addgene :
651 - 700 of 774 citations for Rat Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were cloned into pSpCas9(BB)-2A-GFP (PX458; a gift from Dr. F. Zhang, Broad Institute of Massachusetts Institute of Technology; #48138, Addgene, Watertown, MA).
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bioRxiv - Neuroscience 2023Quote: ... and AAV encoding the cre-inducible excitatory designer receptor human M3 muscarinic receptor (hM3Dq; AAV2-Syn-DIO-hM3Dq-mCherry; Addgene, Watertown, MA) or fluorophore control (AAV2-Syn-DIO-mCherry ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5, 1.7×e13 gene copies/mL) or pAAV-hSyn-EGFP (#50465, AAV5, 1.2×e13 gene copies/mL) (Addgene, Inc., Watertown, MA, USA) were diluted to 8.5×e11 gene copies/mL using PBS (Gibco® Dulbecco’s phosphate-buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... Two small holes were made in the skull above the injection sites and 200nl (100nl/min) of AAV8-hSyn-DIO-mCherry or AAV8- hSyn-DIO-hm4Di-mCherry virus (Addgene, Watertown, MA) was infused in both hemispheres of the PVN using a 1µL Hamilton syringe (0° angle ...
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bioRxiv - Neuroscience 2023Quote: ... The oligos pairs encoding the 20-nt guide sequence were annealed and ligated into the pSpCas9(BB)-2A-Puro (PX459) V2.0 vector (Addgene, Watertown, MA, US) and then amplified in chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: ... Each target sgRNA was cloned into the guide RNA-Cas co-expression plasmid px330-U6-Chimeric_BB-CBh-hSpCas9 vector (Addgene, Watertown, MA, USA). A mixture of the four cloned plasmids was microinjected into the pronuclei of C57BL/6 J fertilized eggs ...
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bioRxiv - Molecular Biology 2024Quote: The HTLV-1 CA (Gag amino acids 132-349) was cloned into the 6×His SUMO bacterial expression plasmid (pHYRSF53, Addgene, Watertown, MA), with mutants created by using the Gibson assembly procedure ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth, Addgene, Watertown, MA) into the LC ...
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bioRxiv - Cancer Biology 2019Quote: The CRISPR kit used for constructing multiplex CRISPR/Cas9 vectors was a gift from Takashi Yamamoto (Addgene kit #1000000054). Guide RNAs targeting LRP5 and LRP6 were designed using the Zhang Lab Optimized CRISPR Design Tool (http://crispr.mit.edu) ...
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bioRxiv - Plant Biology 2022Quote: ... a modular cloning system was employed using MoClo Tool Kit and MoClo Plant Parts kit (Addgene, Supplemental Table S3) (Weber et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and gifted by Dr. Alan Howe (University of Vermont). The Fyn construct in pRK5-Entry (Mariotti et al. 2001) was acquired from AddGene (Cambridge, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The SEP-GluA1 construct was generated by Dr Roberto Malinow (University of California, San Diego) and obtained from Addgene (Watertown, MA: Plasmid #24000).
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bioRxiv - Molecular Biology 2020Quote: ... At least two independent sets of oligonucleotide pairs for gene knockdown of human MTs and the transcription factor MTF-1 (supplementary table S4) were synthesized and cloned into the pLKO.1-TCR vector (Addgene, Cambridge, MA, USA); the same vector was also used as an empty vector control ...
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bioRxiv - Molecular Biology 2021Quote: ... The obtained pGEM-T-RBM8A plasmid was cleaved with Sfi I and subcloned into the Sfi I site of pSBtet-GP vector (Addgene, Watertown, MA, USA) [25].
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bioRxiv - Neuroscience 2020Quote: Stereotaxic injection of AAV-EF1a-DIO-HChR2(H134R)-eYFP (serotype 2/1 or 2/9, U. Penn Vector Core, Philadelphia, PA, USA or Addgene, Watertown, MA, USA) was performed in 6-8 weeks old DAT-IRES-Cre or FoxP2-Cre transgenic mice ...
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bioRxiv - Cell Biology 2020Quote: Stably Cas9-expressing HepG2 cells were transduced with genome-wide lentiviral pooled CRISPR/Cas9 knockout library (Addgene #73178) (Brunello; Cat# 73178-LV) (Addgene, Inc., Watertown, MA) containing 76,441 sgRNAs ...
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bioRxiv - Cell Biology 2022Quote: ... The control virus with VSV-G as the tropism and expression of mCherry was generated by co-transfection of pLV-mCherry and pMD2.G vector (Addgene, Watertown, MA, USA) into the Phoenix cells ...
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bioRxiv - Cancer Biology 2022Quote: ... was purchased from VectorBuilder (Chicago, IL, USA) and the packaging plasmid psPAX2 (#12260) and envelope plasmid pMD2.G (#12259) were obtained from Addgene (Watertown, MA, USA). The plasmid GFP-pLVTHM (#12247 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Single-guide RNA (sgRNA) for the target sequence and Cas9 mRNA were prepared by using the pDR274 vector (plasmid 42250, Addgene, Watertown, MA, USA) and pCS2+hSpCas9 vector (plasmid 51815 ...
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bioRxiv - Neuroscience 2021Quote: ... rats were unilaterally injected with 500 nL of either AAVretro-Cre (gift from Janelia Farms, Ashburn VA, US) or AAV2retro-pmSyn1-EBFP-Cre (Addgene, Watertown, MA, USA) into the RMTg (AP ...
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bioRxiv - Developmental Biology 2021Quote: The Lgr6 overexpression plasmid (pCAG-Lgr6) was constructed by replacing the GFP cassette in the control vector (pCAG-GFP; plasmid #11150, Addgene, Cambridge, MA, USA) with the mouse Lgr6 coding sequence ...
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bioRxiv - Neuroscience 2022Quote: Chemogenetic manipulation of SNc DA neurons was achieved through stereotaxic bilateral infusion of a AAV5-hSyn-DIO-hM4D(Gi)-mCherry (1012 particles/mL, plasmid #44362, Addgene, Watertown, MA, USA) or AAV5-hSyn-DIO-mCherry (1012 particles/mL ...
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bioRxiv - Plant Biology 2022Quote: ... while the full-length coding sequence of HlMYB7 was cloned into the prey vector pGADT7-GW [DNA-binding domain (BD)] (Addgene Inc, MA, USA) using the In-Fusion Snap Assembly Kit (Takara Bio) ...
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bioRxiv - Neuroscience 2023Quote: ... each of these viruses were mixed in a 4:1 ratio with AAV8-Ef1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene; Watertown, MA, USA) and injected with 300 nL per side ...
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bioRxiv - Cancer Biology 2023Quote: The Human Brunello v2 CRISPR KO pooled library (76,441 gRNAs, targeting 19,114 genes, including 1,000 controls) was bought from Addgene (Watertown, MA, USA, Addgene ID: 73179). Plasmid library were amplified by electro-transformation (Lucigen ...
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bioRxiv - Cell Biology 2022Quote: ... containing the sgRNA sequence targeting the coding region of the sec11a and sec11c genes were inserted into the pSpCas9(BB)-2A-Puro (PX459) vector (#48139; Addgene, Watertown, MA, USA) (digested with BbsI ...
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bioRxiv - Cell Biology 2023Quote: ... or a modified version of the mouse genome (GRCm38/mm10) containing the mRNA sequence for tdTomato from the ROSA-Ai9 targeting vector (#22799, Addgene, Watertown, MA, USA) to facilitate identification of GLASTAi9 cells in the scRNA-seq datasets.
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bioRxiv - Bioengineering 2023Quote: ... we followed the protocol of our previous study20 to transfected 6 individually isolated fibroblast lines with 4 μg of episomal plasmid (#58527; Addgene, Watertown, MA, USA) using the NucleofectorTM II with the A-024 program (Amaxa ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of DISC1 were subcloned from existing vectors into pENTR1A no ccDB [36] (Dr. Eric Campeau, via Addgene, Watertown, MA, USA, clone 17398) at the SalI and XbaI sites ...
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bioRxiv - Cell Biology 2024Quote: ... pLenti-PGK-Neo-PIP-FUCCI (PIP-FUCCI) (Cat. NO. 118616) and pLenti PGK Neo DEST (empty vector) (Cat. NO. 19067) were from Addgene (Cambridge, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The oligo pool containing the sgRNA sequences was synthesized by Genewiz (Suzhou, Jiangsu, China) and cloned into lenti-guide-puro (#52963; Addgene, Watertown, MA, USA) as previously described51 ...
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bioRxiv - Cancer Biology 2024Quote: HEK293 cells were cultured in 6-well plates and then transfected with 4 μg of previously described plasmid vectors encoding ER stress reporter genes ATF4-mS (#115970, Addgene, Cambridge, MA, USA), XBP1-mN (#115971) ...
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bioRxiv - Molecular Biology 2024Quote: ... two sets of chemically synthesized DNA containing the target sequence (Table S6, Fig. S8) were subcloned between the EcoRI and BamHI sites in the pBluescript SK(+) vector (Addgene, Watertown, MA, USA) and designated “pBlue-SapiroTarget” and “pBlue-KazanTarget” ...
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bioRxiv - Neuroscience 2024Quote: Vectors coding the dCas9-DNMT3ACD-DNMT3LCD-3xFLAG fusion gene (contains the enzymatic group of the DNA methyltransferase DNMT3) and the control plasmid pCMV-dCas9-mD3A (#78257, Addgene, Watertown, MA, USA) were used as described in 14,22 ...
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bioRxiv - Cancer Biology 2024Quote: ... pairs of sgRNAs were generated using the CRISPick tool from the Broad Institute 60,61 and sequentially cloned into vector px333 (Addgene, #64073, Cambridge, MA, USA). px333 contains two sgRNA cassettes with unique digestion sites for BbsI and BsaI ...
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bioRxiv - Plant Biology 2021Quote: ... the kit (Golden Gate TALEN and TAL Effector Kit 2.0) consisting of 86 library vectors was ordered from Addgene (www.addgene.org). To assemble the dTALe harboring 16 repeats ...
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bioRxiv - Plant Biology 2020Quote: ... The following binary plasmids were assembled by Golden Gate cloning using the MoClo Tool Kit for plants (Addgene kit #1000000044) (Weber et al. ...
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bioRxiv - Bioengineering 2022Quote: Cas9 and FE expression vectors were constructed using the pX330A and pX330S vectors contained in Multiplex CRISPR/Cas9 Assembly System Kit (Kit #1000000055, Addgene)48 with some modifications ...
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bioRxiv - Cell Biology 2023Quote: ... The TRUPATH kit was purchased from Addgene (#1000000163) and was a gift from Bryan Roth ...
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bioRxiv - Synthetic Biology 2023Quote: ... a gift from John Dueber (Addgene kit # 1000000061). The constructs encoding sequential incorporation of serine in place of tyrosine in the FUS IDR was based on previously published sequences66 ...
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bioRxiv - Neuroscience 2020Quote: ... The plasmid kit used for the generation of TALENs was a gift of Daniel Voytas and Adam Bogdanove (Addgene kit #1000000016). Plasmid encoding assembled TALENs was linearized with SacI ...
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bioRxiv - Plant Biology 2020Quote: ... GFP tagged OsHIPP19 and OsHIPP20 were generated by Golden Gate methods (Engler et al. 2008) using MoClo Plant Parts Kit and MoClo Plant Tool Kit (Addgene, UK). OsHIPP19 and OsHIPP20 were cloned into pICH47732 with a GFP tag at the N-terminus ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned the dgn-1 promoter and AcNPV p10 3’UTR into the vector pPD49.26 (from the Andrew Fire plasmid kit, Addgene Kit #1000000001) to generate plasmid pNTC2 ...
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bioRxiv - Cell Biology 2023Quote: ... were constructed in according to Golden Gate TALEN assembly protocol and using the Golden Gate TALEN and TAL Effector Kit 2.0 (Addgene, Kit #1000000024). CIscript-GoldyTALEN was a gift from Daniel Carlson & Stephen Ekker (Addgene ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid construction was performed by modular cloning using the MoClo Tool Kit and the MoClo Plant Parts Kit (Addgene, 28–30). A detailed MoClo protocol and the list of vectors used can be found in 18.
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bioRxiv - Biochemistry 2024Quote: Plasmids encoding GPCRs were obtained from various sources (as indicated Key Resources Table) or subcloned into pcDNA3.1 from the PRESTO-Tango GPCR kit (Addgene Kit #1000000068) as described next ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Cas9 protein was produced by the Weizmann Institute of Science Protein Purification Unit using the pET-28b-Cas9-His (Alex Schier Lab Plasmids, Addgene, Cambridge, MA, United States) as a template ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were co-transfected using lentiviral packaging plasmids (psPAX2 and pMD2.G) with a PLKO.1-blast plasmid (Addgene, Cambridge, MA, USA, #26655) containing shSmad2 or shYAP ...
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bioRxiv - Cancer Biology 2022Quote: For CRISPR/Cas9 knockout AMPK sgRNA plasmids targeting exon 1 of AMPK α1 and αβ (pX462-hPRKAA1-gRNA, pX462-hPRKAA2-gRNA, #74374-74377) were purchased from Addgene (Watertown, MA, USA). LNT-229 ...
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bioRxiv - Immunology 2020Quote: ... devil NLRC5 was amplified from pAF105 with overlapping ends to the 5’ and 3’ SfiI sites of the Sleeping Beauty transposon plasmid pSBbi-BH42 (a gift from Eric Kowarz; Addgene # 60515, Cambridge, MA, USA) using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB) ...