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Citations for Addgene :
451 - 500 of 774 citations for Rat Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: CDS of zebrafish fto mRNA was synthesized and inserted into the pCS2+ plasmid (Addgene, Watertown, MA, USA) to get the linearized pCS2+-FTO plasmid for in vivo transcription ...
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bioRxiv - Microbiology 2023Quote: HeLa S3 cells were transduced with a Streptococcus pyogenes (sp)Cas9 expressing lentivirus (#52962; Addgene, Watertown, MA) at an MOI of 20 transduction units (TU)/cell ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred into the destination vectors pDEST-CMV-C-EGFP (#122844; for Lamp1; Addgene, Watertown, MA, USA) or pDest-mCherry-N1 (#31907 ...
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bioRxiv - Neuroscience 2024Quote: ... The dLight virus (700 nL of AAV5-hSyn-dLight 1.2; Addgene, Watertown, MA; Catalog No.111068-AAV5) was injected unilaterally into the NAc core (in mm ...
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bioRxiv - Neuroscience 2023Quote: ... AAV vector encoding for inhibitory DREADD (AAV5-SYN1-hM4Di-HA 1.7*1013 GC/ml (Addgene, Watertown MA)) was injected into the bilateral basolateral amygdala stereotaxically ...
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bioRxiv - Synthetic Biology 2023Quote: ... We used DNA parts provided with the EMMA cloning kit (Addgene kit # 1000000119) and generated DNA fragments by PCR using Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... The guides were cloned into the plasmids pX330 or pX548 (obtained from Zhang Laboratory via Addgene; Cambridge, MA) and the PCR products were transcribed with the T7 MEGAshortscript kit (Life Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... Str-KDEL_neomycin was a gift from Franck Perez (Addgene, Watertown, MA, Addgene plasmid # 65306; http://n2t.net/addgene:65306; RRID:Addgene_65306). This construct allowed subsequent labelling of well-fixed ER using fluorescent biotin ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids pHSG-PCNA2 and pHSG-PCNA3 were a gift from Teruyuki Nagamune obtained through Addgene (Cambridge, MA, USA) (Addgene plasmid # 66126; http://n2t.net/addgene:66126; RRID:Addgene_66126) and (Addgene plasmid # 66127 ...
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bioRxiv - Physiology 2020Quote: ... The murine Ntf3 cDNA was cloned into a Rosa26 locus targeting vector 25 purchased from Addgene (Cambridge, MA). Key features of the complete targeting construct as depicted in Suppl ...
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bioRxiv - Neuroscience 2021Quote: ... http://n2t.net/addgene:44361; RRID:Addgene_44361 and viral prep # 44362-AAV2; http://n2t.net/addgene:44362; RRID:Addgene_44362; Addgene, Watertown, MA)47 ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced using 293T cells with the second-generation packaging system psPAX2 (#12260, Addgene, Watertown, MA, USA) and PMD2 ...
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bioRxiv - Cell Biology 2022Quote: ... The vector pSpCas9(BB)-2A-Puro (PX459) V2.0 was provided from Feng Zhang (plasmid 62988; Addgene, Cambridge MA) (Ran et al ...
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bioRxiv - Neuroscience 2021Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA) to drive expression preferentially in principal neurons ...
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bioRxiv - Neuroscience 2021Quote: ... AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (serotype AAV1, viral titer ≥ 5×1012 vg/mL; Addgene, Watertown, MA, USA), pAAV-hSyn-hM4D(Gi)-mCherry (serotype AAV retrograde ...
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bioRxiv - Neuroscience 2020Quote: ... 10 animals received rejections of AAV5-CaMKIIa-hM4Di-mCherry (titer 9.5×10^12 GC/ml; Addgene, MA, USA), while eight animals received injections of a non-DREADD expressing viral control AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml ...
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bioRxiv - Physiology 2019Quote: ... envelope vector pMD2.G (ID #12259) and scrambled shRNA plasmid (sc: ID1864) were obtained from Addgene (Cambridge, MA). HEK293T cells in 10 cm dishes were transfected using 50µL 0.1% Polyethylenimine ...
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bioRxiv - Immunology 2019Quote: ... Lentivirus was generated by calcium phosphate transient transfection of 293T cells using psPAX2 packaging (Addgene, Cambridge, MA, USA) and pMD2.G (Addgene ...
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bioRxiv - Genomics 2021Quote: ... pAAV-EF1a-Cre was a gift from Karl Deisseroth (Addgene, Watertown, MA; plasmid #55636; http://n2t.net/addgene:55636; RRID:Addgene_55636). First ...
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bioRxiv - Cancer Biology 2020Quote: Expression plasmid encoding N-terminally Flag-tagged hFOXO1-3A (#13508) was purchased from Addgene (Cambridge, MA, pCDNA3 backbone). FOXO1-3A has Ala residue substitutions at Thr-24 ...
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bioRxiv - Genomics 2022Quote: ... 5 μg DNA was incubated with1.75 μg pMD2.G and 3.25 μg pCMV-dR8.91 (Addgene, Watertown, MA; #12259). On the next day ...
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bioRxiv - Neuroscience 2022Quote: ... 100-200 nl virus solution of 3-5 × 1010 genome copies containing AAV5.gfaABC1d.Lck-GCaMP6f (Addgene, MA, USA) were injected at two or three sites in the craniotomy site at the cerebellar cortex (Vermis Lobule 4/5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Genetic depletion of mouse RhoA or AKT1 in vivo was conducted using pSECC (#60820; Addgene, Watertown, MA, USA), a lentiviral-based system that combined both the CRISPR system and Cre recombinase ...
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bioRxiv - Neuroscience 2022Quote: ... the viral vector pAAV-CamKII-ArchT-GFP (titer: 1.9×1013 genome copies per ml, Addgene, Watertown, MA, USA) was injected bilaterally 0.45 mm from the midline at 1 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (between passage 9-11) were transfected with lentiviral packaging vector psPAX2 (#12259, Addgene, Watertown, MA, USA), the envelope vector pMD2.G (#12260 ...
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bioRxiv - Cell Biology 2023Quote: ... which was a gift from Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA, USA) (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). GFP-positive cells were sorted after 24 h of transfection and plated ...
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bioRxiv - Cell Biology 2023Quote: ... The lentiviral packaging plasmids pMD2.G and psPAX2 (Didier Trono; 12259, 12260) were obtained from Addgene (Cambridge, MA). Four 15-cm culture plates containing HEK293T cells at 80% confluency were each transfected with 8 μg pTRIPZ-OGT-FH ...
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bioRxiv - Biochemistry 2023Quote: ... and S545A (HS-BG) constructs were cloned into a pHIV-dTomato lentiviral backbone (Plasmid #21374, Addgene, Cambridge, MA) by the Center for Targeted Therapeutics Core Facility at the University of South Carolina (Columbia ...
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bioRxiv - Molecular Biology 2023Quote: ... A recombinant DNA pcDNA3/Myc-DNMT1 was a gift from Arthur Riggs (Addgene plasmid #36939 Watertown, MA, USA) (Li et al. ...
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bioRxiv - Genetics 2023Quote: ... oligonucleotides coding for gRNA sequences were in vitro aligned and cloned into pX330 plasmid (Addgene, Watertown, MA 02472) for co-expression of Cas9 and a corresponding gRNA ...
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bioRxiv - Neuroscience 2023Quote: ... brain slices were cut from DbhCre mice bilaterally injected with AAV1-hSyn1-SIO-stGtACR2-FusionRed (Addgene, MA, USA) into the LC and allowed to recover for 5-8 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA). Virus (0.5 µl ...
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bioRxiv - Plant Biology 2024Quote: 35S:GUS-pMAS:GFP was modified by replacing the 35S promoter with the MAS promoter from pGFPGUSPlus (Plasmid #64401, Addgene) (He et al. ...
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bioRxiv - Synthetic Biology 2023Quote: The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059). CIDAR MoClo Extension ...
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bioRxiv - Biochemistry 2022Quote: ... The PRESTO-Tango plasmid kit was a gift from Bryan Roth (Addgene kit # 1000000068).
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bioRxiv - Synthetic Biology 2023Quote: ... The MoClo-YTK plasmid kit was a gift from John Dueber (Addgene kit # 1000000061). Enzymes used were from New England Biolabs unless stated otherwise ...
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bioRxiv - Molecular Biology 2023Quote: The CRISPaint gene tagging kit was a gift from Veit Hornung (Addgene kit # 1000000086). To generate a targeting construct for AGO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All GPCR plasmids originated from the Roth lab PRESTO-Tango kit (Addgene, Kit #1000000068) and were amplified in E ...
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bioRxiv - Neuroscience 2020Quote: ... Feng Zhang (Addgene kit #1000000019). Once assembled into a destination vector ...
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bioRxiv - Neuroscience 2019Quote: ... Virus injections per mouse pup were in a total volume of 4μL of AAV9-syn-GCaMP6s (Addgene, MA, USA) with a titer of 1Χ1013 vg/mL ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids pHSG-PCNA2 and pHSG-PCNA3 were a gift from Teruyuki Nagamune obtained through Addgene (Cambridge, MA, USA) (Addgene plasmid # 66126 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pAR-Ec611 (ArEc-Rev1-611) plasmid harboring medium-error-rate polymerase TP-DNAP1_611 (≥10−6 s.p.b.) was obtained from Addgene (Watertown, MA).
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bioRxiv - Cancer Biology 2020Quote: CRISPR-Cas9-mediated knockout was delivered by pSpCas9 (BB)-2A-GFP (PX458) (Plasmid #48138, Addgene, and Cambridge, MA, USA). Sequences of sgRNAs are listed in the following supplementary table S2 ...
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bioRxiv - Neuroscience 2021Quote: MSCV (Murine Stem Cell Virus)-CreERT2 puro plasmid was a gift from Tyler Jacks (Addgene, Watertown, MA; plasmid # 22776; http://n2t.net/addgene:22776; RRID: Addgene_22776). pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371 ...
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bioRxiv - Cell Biology 2021Quote: ... Sequence analysis and primer design was done in Benchling [68] All of the plasmids were from Addgene (Watertown, MA) and primers (Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well plates and transfected 24h after with pMXs GFP-LC3-RFP (#117413, Addgene, MA) or with Polyinosinic-polycytidylic acid sodium salt ...
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bioRxiv - Genetics 2022Quote: ... Each 20 nt gRNA sequence was each cloned into a PX459 (Ran et al., 2013) (Addgene 62988, Watertown, MA) backbone digested with BbsI (Thermo FD1014) ...
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bioRxiv - Neuroscience 2020Quote: Adeno-associated viruses (AAVs) carrying Cre-inducible (double-inverse orientation; DIO) transgenes were purchased from Addgene (Watertown, MA, USA). We used stimulatory AAV8-hSyn::DIO-hM3Dq-mCherry (4.0e12 GC/ml titer ...
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bioRxiv - Neuroscience 2020Quote: ... We delivered AAV8.hSyn.hM4Di.mCherry (hM4Di) or AAV8.hSyn.mCherry (mCherry) targeting the BLA and contralateral NAc core (Addgene, Watertown, MA) via a micropump (UltraMicroPump III ...
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bioRxiv - Immunology 2021Quote: ... and a separate master mix was prepared comprising 1 mL Opti-MEM 8 µg PSPAX (12260, Addgene, Watertown, MA), 2 µg PMD2G plasmids (12259 ...