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Citations for Addgene :
201 - 250 of 829 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... the expression plasmids (pRha-ABE8e-NRCH, Addgene #165417; and pABE8e-protein, Addgene #161788) were transformed into BL21Start DE3 competent cells (Thermo) ...
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bioRxiv - Biochemistry 2020Quote: ... and for luciferase assays a ratio of 1:9:10 HA-Clover plasmid:pcDNA(-):HRE-Luciferase (Addgene #26731) was used (physiological expression levels) ...
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bioRxiv - Cell Biology 2023Quote: The photoactivation-based fusion assay (Karbowski et al., 2014) was conducted by expressing Mito-PAGFP (Addgene #23348) in primary human fibroblasts ...
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bioRxiv - Molecular Biology 2019Quote: DNA sequences corresponding to sgRNAs were cloned into LentiGuide-Puro (Addgene: 52963) or a modified form of LentiCRISPRv2 (Addgene ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 2 ng/μl RNase-free plasmid DNA (pX330, Addgene #42230) in a 15 ml Falcon tube ...
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bioRxiv - Molecular Biology 2020Quote: 2 μl RNase-free plasmid DNA (pX330, Addgene #42230, 100 ng/μl),
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847) and an NheI/PmeI-digested piggyBac dox inducible expression vector PB-TRE-Cas91 including a puromycin selection marker ...
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bioRxiv - Neuroscience 2019Quote: ... 125 ng of each DNA construct expressing BFP and ITGB1 (Addgene 51920) and 1.25μl of Lipofectamine LTX was used in each well (in a 24 well plate) ...
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bioRxiv - Microbiology 2020Quote: ... and a DNA mixture of 11.9 μg of pMD2.G (Addgene #12259), 18.2 μg of psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2022Quote: ... DCX DNA was cloned into the N-Terminal pFLAG 3 vector (Addgene) by restriction digest using NotI and BamHI restriction enzymes ...
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bioRxiv - Developmental Biology 2020Quote: ... 2a (full plasmid sequence and DNA to be deposited at Addgene # reference) to the Rosa26 locus of mouse ES cells with G4 background following standard procedures 33 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1j (full plasmid sequence and DNA to be deposited at Addgene # reference) to the Rosa26 locus using the same procedure (To be described in detail elsewhere) ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag-tagged DAXX DNA were amplified from plasmid Flag-Daxx/pRK5 (Addgene) and assembled into pCDH-EF1α-MCS-IRES-Puro vector (System Biosciences).
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bioRxiv - Molecular Biology 2022Quote: DNA corresponding to sgRNAs was cloned into LentiCRISPRv2 using BamHI (Addgene, #52961). sgRNA target sequences and their validations can be found in Supplementary Table 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... IκBα DNA was PCR amplified from pBABE-GFP-IκBα-wt (Addgene, 15263) and was digested and cloned into pET28-CBP-His6 ...
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bioRxiv - Neuroscience 2023Quote: ... with plasmid DNA from either FUW-TetO-Ngn2-P2A-puromycin (#52047, Addgene) or FUW-M2rtTA (#20342 ...
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bioRxiv - Molecular Biology 2022Quote: ... synthesized by Integrated DNA Technology (IDT) and cloned into pX461 vector (Addgene). Donor pUC19-based vectors containing a selectable marker ...
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bioRxiv - Genetics 2023Quote: ... DNA plasmids used for CLYBL locus targeting were pC13N-iCAG.copGFP (Addgene 66578), pZT-C13-L1 (Addgene 62196) ...
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bioRxiv - Cell Biology 2023Quote: ... an Fmr1 exon 3 DNA oligonucleotide was inserted into pLentiCRISPR (Addgene, 49535) adapted from published methods [12] ...
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bioRxiv - Pathology 2023Quote: Cre recombinase DNA was amplified by PCR using pCAG-Cre (Addgene #13775) as a template ...
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bioRxiv - Genomics 2023Quote: ... 10 μg payload DNA and 10 μg pCAG-iCre plasmid (Addgene #89573) per transfection ...
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bioRxiv - Genetics 2023Quote: ... 2.5 μg of DNA (1 μg of mCherry-expressing plasmid (Addgene, 72264), and 1.5 μg of either pcDNA4/TO-eGFP ...
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bioRxiv - Cancer Biology 2024Quote: ... and mTOR DNA constructs were obtained from Addgene (plasmid #12133, #14129, #1861)24 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were cloned into the pNvT-MHC::mCherry vector (Addgene #67943) using PacI (NEB #R0547 ...
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bioRxiv - Neuroscience 2023Quote: The plasmid RHCglo used for minigene assay was a gift from Thomas Cooper (Addgene plasmid #80169 ; http://n2t.net/addgene:80169 ; RRID:Addgene_80169) (49) ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were labeled for live assays prior to incorporation into miBrains using an AAV (pAAV-CAG-tdTomato, Addgene or AAV9-CAG-GFP ...
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bioRxiv - Developmental Biology 2024Quote: ... The AAV-CMV-GFP plasmid used for neurite outgrowth assay was a gift from Connie Cepko (Addgene, #67634).
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bioRxiv - Cancer Biology 2021Quote: ... For expression of the Cas9 protein the lentiCas9-Blast expression vector was used (Addgene plasmid #59262 ...
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bioRxiv - Cell Biology 2020Quote: mCherry-ER fusion protein was amplified by PCR from mCherry-ER-3 plasmid (Addgene) and cloned into Gateway modified pBABE (in house) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs of the human proteins were cloned into pTT5 based expression vectors (Addgene #52355). The constructs were tagged with Twin-Strep-tag (SII ...
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bioRxiv - Neuroscience 2021Quote: ... and SAD-B19 helper proteins (N, 52.11 mg, Addgene 59924; P, 30.15 mg, Addgene 59925 ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 μg of viral entry protein VSV-G plasmid pMD2.G (Addgene; 12259) were mixed into 600 μl of serum-free DMEM ...
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bioRxiv - Genomics 2019Quote: ... we cloned the effector proteins (PguCas13b: Addgene 103861, PspCas13b: Addgene 103862, RfxCas13d: Addgene 109049) and their direct repeat sequences (PguCas13b ...
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bioRxiv - Genomics 2019Quote: ... we cloned the effector proteins (PguCas13b: Addgene 103861, PspCas13b: Addgene 103862, RfxCas13d: Addgene 109049) and their direct repeat sequences (PguCas13b ...
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bioRxiv - Immunology 2021Quote: Recombinant HA (rHA) proteins were expressed using the pcDNA 3.1+ plasmid (Addgene, Watertown, MA). Each HA gene was truncated by removing the transmembrane (TM ...
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bioRxiv - Microbiology 2021Quote: The Plasmid expressing SARS CoV-2 Spike protein (Wuhan isolate) was procured from Addgene with 19 nineteen amino acids deletion at C- terminal that enables efficient lentiviral packaging ...
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bioRxiv - Biophysics 2020Quote: The plasmid for membrane scaffold protein was MSP1D1 in pET28a was purchased from Addgene and prepared as previously described (Hagn et al. ...
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bioRxiv - Neuroscience 2019Quote: ... with different fluorescent protein genes and helper plasmids (3 μg pcDNA-SADB19N (Addgene, 32630), 1.5 μg pcDNA-SADB19P (Addgene ...
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bioRxiv - Immunology 2020Quote: ... and VSV-G protein-encoding plasmid pMD2.G were obtained from Addgene (Watertown, MA).
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bioRxiv - Developmental Biology 2019Quote: ... RNPs of Cas9 protein (Cas9-mCherry, pMJ293 (Burger et al., 2016) (available from Addgene)) and sgRNA were in vitro-assembled for 10 min at 37°C in 300 mM KCl to ensure maximum cleavage efficiency and microinjected into the cell of one-cell stage embryos (Burger et al. ...
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bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032 ...
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bioRxiv - Microbiology 2021Quote: ... A VSV G protein expression plasmid was obtained from Addgene (Watertown, MA; Cat.# 8454).
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bioRxiv - Neuroscience 2022Quote: ... The recombinant AAV vector was pseudotyped with AAV5 capsid protein and packaged by Addgene.
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bioRxiv - Neuroscience 2023Quote: ... and green fluorescent protein (pAAV2-hSyn-eGFP, Addgene #50465, 2.2 x 1013 GC/ml) under the human synapsin promoter were obtained from Addgene ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids for SARS-CoV-2 structural proteins were purchased from Addgene (Appendix Table 2). Lentiviral supernatant was collected according to the manual using 293FT cells ...
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bioRxiv - Neuroscience 2023Quote: ... Control mice were infused with a red fluorescent protein (excitation: AAV8-Ef1a-mCherry, Addgene #114470 ...
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bioRxiv - Molecular Biology 2023Quote: ... CAST I-B system’s proteins and inverted repeat constructs were sub-cloned from Addgene plasmids #168137 and #168146 to generate pIF1003.
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
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bioRxiv - Microbiology 2023Quote: ... plasmid containing human codon-optimized Streptococcus pyogenes Cas9 protein and Stk3 (Mst2, Addgene #75975) gRNA was used to dually target Mst1 and Mst2 ...
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bioRxiv - Developmental Biology 2021Quote: ... The target DNAs were inserted into the BsaI site of pDR274 (Addgene; 39) as vectors for in vitro transcription of gRNAs ...