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Citations for Addgene :
151 - 200 of 829 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: For validation experiments we introduced the fluorescent protein EBFP2 (source: Addgene 54665) driven by the dpse enhancer and the CG13116 promoter in the RD-seq plasmid backbone to be able to gate for transfected cells in flow cytometry (Suppl ...
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bioRxiv - Immunology 2023Quote: ... Plasmids expressing the SARS-CoV-2 S protein were obtained from Addgene: pcDNA3.3-SARS2-B.1.617.2 (Delta ...
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bioRxiv - Immunology 2023Quote: ... 700 ng/mL of Protein A/G-MNase (plasmid Addgene ID:123461) were added to the immunocomplexes ...
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bioRxiv - Neuroscience 2024Quote: Enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1) was commercially purchased (Addgene). The fluorescent protein mKate was inserted into a pcDNA3.1(- ...
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bioRxiv - Microbiology 2021Quote: ... WT Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was always >80-90% ...
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bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420 ...
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bioRxiv - Cancer Biology 2022Quote: The mismatch repair assay was performed as described previously57 using plasmids pGEM5Z (+)-EGFP (Addgene, cat #65206) and p189 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: Luciferase assays were performed in HEK293T cells using the YAP/TAZ reporter 8xGTIIC-lux (Addgene, 34615). Renilla-Luc (0.05 µg/well ...
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bioRxiv - Biochemistry 2023Quote: A Halo-based assay to measure mitophagy was recently developed using pSu9-Halo-mGFPplasmid (Addgene, 184905) 53 ...
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bioRxiv - Genomics 2019Quote: ... from mouse tail genomic DNA and pX330 plasmid (Cat. 42230, Addgene), respectively ...
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bioRxiv - Cancer Biology 2019Quote: DNA corresponding to sgRNAs was cloned into LentiGuide-Puro (Addgene: 52963), LentiCRISPRv2 (Addgene ...
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bioRxiv - Genetics 2021Quote: ... 2.5 μg of DNA (1μg of mCherry-expressing plasmid (Addgene, 72264), and 1.5 μg of pcDNA4TO-eGFP ...
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bioRxiv - Cell Biology 2020Quote: ... DNA encoding sgRNA’s were subcloned into pSpCas9-2A-Puro V2.0 (Addgene).
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bioRxiv - Neuroscience 2021Quote: ... DNA templates and shRNA oligoes mentioned above were acquired from Addgene or synthesized from BGI (Shenzhen ...
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bioRxiv - Bioengineering 2021Quote: ... The DNA sequence of GINKO1 was obtained from Addgene (plasmid #113112).
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified Cas9n-NG DNA from pX330-SpCas9-NG (Addgene# 117919), PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mMff-P2A (a gift from David Chan (Addgene plasmid # 44601 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of DIO-EGFP (a gift from Bernardo Sabatini (Addgene plasmid # 37084 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mDrp1-P2A (a gift from David Chan (Addgene plasmid # 34706 ...
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bioRxiv - Molecular Biology 2020Quote: DNA template was amplified from dCas9-P300 expressing plasmid (Addgene #48226) using specific primers for IVT reaction (Supplementary Table S4) ...
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bioRxiv - Molecular Biology 2022Quote: ... Each DNA sequence was inserted into either pCDNA3-mRuby2 (Addgene#40260) or pCDNA3-Clover (Addgene#40259) ...
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bioRxiv - Cell Biology 2022Quote: ... mApple and TfR DNA was obtained from mApple-Lysosomes-20 (RRID:Addgene_54921) and mCherry-TFR-20 (RRID:Addgene_55144 ...
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bioRxiv - Bioengineering 2019Quote: ... 2 µL plasmid DNA (ERmoxGFP43, Addgene Cat. # 68072, 420 ng/µL), and 96 µL of PBS ...
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bioRxiv - Plant Biology 2020Quote: ... a DNA fragment of NLS–mRuby2 (obtained from Addgene plasmid 40260), was amplified and then cloned into the pENTR/D-TOPO vector (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Human codon optimized cas9 DNA was obtained from (Addgene, Plasmid #41815). To generate the knockout cell lines ...
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bioRxiv - Bioengineering 2020Quote: ... melanogaster genomic DNA and a dCas9-VPR fragment amplified from Addgene plasmid #78898 22 with primers 986.C11 and 986.C12 to generate plasmid OA-986B (Addgene #124999) ...
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bioRxiv - Bioengineering 2020Quote: ... melanogaster genomic DNA and a dCas9-VP64 fragment amplified from Addgene plasmid #78897 22 with primers 986.C17 and 986.C18 to generate plasmid OA-986D (Addgene #125001) ...
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bioRxiv - Bioengineering 2020Quote: ... melanogaster genomic DNA and a dCas9-VPR fragment amplified from Addgene plasmid #78898 22 with primers 986.C15 and 986.C12 to generate plasmid OA-986C (Addgene #125000) ...
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bioRxiv - Bioengineering 2020Quote: ... melanogaster genomic DNA and a dCas9-VP64 fragment amplified from Addgene plasmid #78897 22 with primers 986.C20 and 986.C18 to generate plasmid OA-986E (Addgene #125002).
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bioRxiv - Cancer Biology 2021Quote: ... DNA oligos were cloned into Tet-pLKO-puro (Addgene plasmid #21915) vector ...
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bioRxiv - Biochemistry 2022Quote: DNA constructs for the expression of KRAS4B (1-169) (Addgene #159539) and RAF1 (52-131 ...
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bioRxiv - Cell Biology 2023Quote: ... and DNA was purified before ligation into pJR103 (Addgene plasmid #187242). The ligated DNA was precipitated using isopropanol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and corresponding DNA oligos were ligated into pCRISPR-Lenti-v2 (RRID:Addgene_52961). To test the efficiency of the guides ...
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bioRxiv - Neuroscience 2024Quote: ... pT3TS-nCas9n template DNA (Addgene, plasmid #46757; Jao et al., 2013) was digested with XbaI (R0145S ...
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bioRxiv - Cell Biology 2023Quote: ... HA-hTRIM28 was removed from pKH3-TRIM28 plasmid DNA (Addgene, #45569) via digestion with NheI and MluI in CutSmart buffer (New England Biolabs) ...
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bioRxiv - Plant Biology 2019Quote: ... Green fluorescent protein (GFP) from the Gateway entry vector pENTR4-GFP-C1 (Addgene) was transferred to pYES-DEST52 and pAG426GAL-ccdb using Gateway LR Clonase ...
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bioRxiv - Genomics 2019Quote: ... we cloned effector proteins (PguCas13b: Addgene 103861, PspCas13b: Addgene 103862, RfxCas13d: Addgene 109049) and their direct repeat (DR ...
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bioRxiv - Genomics 2019Quote: ... we cloned effector proteins (PguCas13b: Addgene 103861, PspCas13b: Addgene 103862, RfxCas13d: Addgene 109049) and their direct repeat (DR ...
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bioRxiv - Molecular Biology 2019Quote: ... The nanodisc scaffold protein MSP1D1 was expressed and purified using pMSP1D1 (Addgene #20061) as described previously (Denisov et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Retrograde adeno associated viruses encoding green fluorescent protein (Addgene, Catalog number 50465-AAVrg) or tdtomato (Addgene ...
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bioRxiv - Physiology 2021Quote: ... Fluorescent protein sequences were PCR-derived from pmVenus(L68V)-mTurquiose2 (Addgene plasmid #60493), Gamillus/pcDNA3 (Addgene plasmid #124837) ...
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bioRxiv - Cell Biology 2020Quote: ... SpCas9 protein generated from the expression plasmid pET-NLS-Cas9-6xHis (Addgene #62934) was purified according to (Zuris et al. ...
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bioRxiv - Developmental Biology 2021Quote: DR274 gRNA plasmids and Cas 9 protein were from Addgene (Cambridge Massachussets, USA) and New England Biolabs (Ipswich ...
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bioRxiv - Molecular Biology 2020Quote: ... A plasmid expressing anti-CRISPR protein (pEJS581; pCSDest2-AcrIIC4Hpa-FLAG-NLS; Addgene # 113436) was included in the triple-transfection packaging process to maintain intact rAAV:HDR:cleaved plasmids during production ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid pcDNA3-YFP (for YFP protein) was a gift from Doug Golenbock (Addgene plasmid #13033 ...
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bioRxiv - Molecular Biology 2022Quote: ... Brunello library targeting all human protein-coding genes28 was purchased from Addgene (#73179). Quality of the MYC-CRISPR and Brunello libraries was verified by next-generation sequencing on Illumina platform (BGI ...
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bioRxiv - Systems Biology 2023Quote: ... and to amplify the fluorescent proteins from plasmids (SYFP2 from pSYFP2-C1 Addgene #22878 ...
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bioRxiv - Microbiology 2023Quote: ... All other SARS-CoV-2 viral protein-encoding plasmids were obtained from Addgene in the pLVX-EF1a-IRES-puro backbone (Addgene #141367-141370 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs were subcloned into the pMSCV-IRES-green fluorescence protein vector (Addgene #20672) with reference to a report by Masubuchi et al.43 The constructed plasmid was validated by sequencing prior to use.
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bioRxiv - Cancer Biology 2024Quote: Pre-Intact clone was infected with pLV-Azurite (blue fluorescent protein, Addgene, 36086) and Pre-ARSIR1 clone was infected with SGEP-Renilla-713 (GFP ...