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Citations for Addgene :
351 - 400 of 1322 citations for PDGF BB Human P.pastoris since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... eSpCas9-2A-GFP was constructed by subcloning 2A-GFP from pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) into eSpCas9 using EcoRI sites ...
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bioRxiv - Microbiology 2020Quote: The plasmids used for CRISPR-Cas9 gene editing were pSpCas9(BB)-2A-GFP (PX458, Addgene ID 48138) and pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Cell Biology 2020Quote: ... Respective sequences were ligated into expression plasmid pSpCas9(BB)-2A-Puro(PX459)V2.0 (Addgene plasmid ID: 62988) using BbsI (Ran et al. ...
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bioRxiv - Cell Biology 2021Quote: ... gRNAs were cloned in the pSpCas9(BB)-2A-GFP (a gift from Feng Zhang, Addgene plasmid #48138), and pSpCas9(BB)-2A-mCherry ...
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bioRxiv - Cell Biology 2021Quote: The sgRNAs were designed using the CRISPOR online tool (http://crispor.tefor.net/crispor.py) and cloned in the pSptCas9(BB)-2A-Puro(PX459)-V2.0 vector (Addgene #62988). The sgRNA cloning was done according to the protocol from 66.
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bioRxiv - Genetics 2019Quote: ... pSpCas9(BB)-2A-Puro(PX459)V2.0 from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988) was purchased from Addgene.
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bioRxiv - Cancer Biology 2021Quote: ... #2: 5’-caccgTGAAACGGATTCTTCTTTCG-3’) and cloned into the Cas9 plasmids pSpCas9(BB)−2A-Puro (PX459, Addgene #62988) and pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected using Lipofectamine 2000 (Thermo) with the pSpCas9(BB)-2A-puro (px459) V2.0 plasmid (Addgene) containing CRISPR guide-RNAs (gRNAs ...
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bioRxiv - Genomics 2022Quote: ... pSpCas9(BB)-2A-Puro (BX459) v.2.0 (a gift from F. Zhang (Addgene plasmid, 62988; RRID, Addgene_62988)) was used to construct CRISPR/Cas vectors according to the protocol of Ran et al 107 ...
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bioRxiv - Genomics 2022Quote: ... pSpCas9(BB)-2A-Puro (BX459) v.2.0 (a gift from F. Zhang (Addgene plasmid, 62988; RRID, Addgene_62988)) was used to construct CRISPR/Cas vectors according to the protocol of Ran et al 107 ...
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bioRxiv - Immunology 2023Quote: ... Gatad2b (5’-CGTCTAGCACTCATATCCAC-3’) were cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (53) (Addgene plasmid #62988). SgRNAs for CRISPR silencing (sgRipk3 enhP #1 ...
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bioRxiv - Genomics 2023Quote: ... CRISPR/Cas9 nickase gene editing was performed using vector pX335-U6-Chimeric BB-CBh-hSpCas9n(D10A) (Addgene) modified by standard restriction enzyme cloning (BbsI ...
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bioRxiv - Cell Biology 2023Quote: Calu-1ERN1-/-cells were constructed by transfection with pSpCas9n(BB)-2A-Puro (Feng Zhang lab, Addgene #48139) containing sequences for guides targeting the start codon (WGE ID’s 1150311103 and 1150311093) ...
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bioRxiv - Genetics 2023Quote: ... pSpCas9n(BB)-2A-GFP (PX461) was a gift from Feng Zhang (Addgene plasmid #48140; https://www.addgene.org/48140/; RRID:Addgene_48140). Chemically competent OneShot TOP10 Escherichia coli (LifeTechnologies ...
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bioRxiv - Cell Biology 2023Quote: ... and introduced into BbsI-linearised pSpCas9(BB)-2A-GFP vector (a gift from Feng Zhang; Addgene # 48138) using Gibson Cloning kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... pSpCas9(BB)-2A-GFP (PX458) was from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138) (Ran et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs targeting each gene were cloned into the BbsI site of pSpCas9 (BB)–2A–Puro (PX459; Addgene), and cells were transfected with each cloned vector using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... guide RNAs targeting PREPL were designed and cloned into a pSpCas9(BB)-2A-Puro vector (Addgene 48139). For the p.Ser559Ala cell line ...
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bioRxiv - Cell Biology 2024Quote: ... The two sgRNAs were cloned into either the pSpCas9(BB)-2A-GFP (PX458) plasmids (Addgene, Cambridge, USA)31 ...
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bioRxiv - Genomics 2023Quote: ... SgRNAs designed for CRISPR-Cas9 deletions were cloned into the pSpCas9(BB)-2A-GFP vector (Addgene #48138) or into the pSpCas9(BB)-2A-mCherry vector as previously described41 ...
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bioRxiv - Neuroscience 2024Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Broad Institute, Boston, USA) (Addgene plasmid # 62988 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the pSpCas9(BB)-2A-Puro V2.0 (PX459) was a gift from Feng Zhang (Addgene plasmid #62988) (Ran et al ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary gRNAs were annealed and subcloned into the pSpCas9(BB)-2A-GFP (pX-458) vector (#48138; Addgene) between BbsI endonuclease restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). Two sgRNAs were used ...
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bioRxiv - Cell Biology 2020Quote: ... sgRNA was cloned into pSpCas9(BB)-2A-GFP (PX458) vector kindly provided by Feng Zhang (Addgene plasmid #48138) [28] ...
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bioRxiv - Cell Biology 2020Quote: ... and Smo−/− ESCs the following gRNAs sequences were cloned in the pX458_pSpCas9(BB)-2A-GFP vector (Addgene, #48138):
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bioRxiv - Immunology 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138http://n2t.net/addgene:48138;RRID:Addgene_48138).
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bioRxiv - Genetics 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138 ; http://n2t.net/addgene:48138 ; RRID:Addgene_48138). Sequences of sgRNA are shown in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... RD cells were transfected with the all-in-one Cas9/gRNA plasmid pSpCas9 BB-2A-GFP (PX458; Addgene; gRNA target sequence ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... sequences to target the MYC gene in the proximity of the BR-coding region were designed using the online software CRISPR Design Tool (http://crispr.mit.edu/) and cloned as DNA inserts into pSpCas9 (BB)-2A-GFP (PX458) (Addgene plasmid # 48138 ...
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bioRxiv - Neuroscience 2019Quote: ... 5’-CTTCTAAAATGGGTGGCATA-3’ was designed using the CRISPR design tool (http://crispr.mit.edu) from the Zhang laboratory and cloned into plasmid pSpCas9(BB)-2A-Puro (Px459) V2.0 (Addgene, 62988). Plasmid constructs were purified with the Endo-free Plasmid Maxiprep kit (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... upstream and downstream sgRNAs were cloned under an U6 promoter into the pSpCas9(BB)-2A-GFP (Addgene #48138) and the pSpCas9(BB)-2A-mCherry (generated in house ...
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bioRxiv - Genetics 2019Quote: ... The guide sequence was then cloned into vector pX330-U6-Chimeric_BB-CBh-hSpCas9 which was a gift from Feng Zhang (Addgene plasmid # 42230; http://n2t.net/addgene:42230; RRID:Addgene_42230). The T7 promoter was added to the sgRNA template ...
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bioRxiv - Cell Biology 2019Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). HeLa cells stably expressing C2-His6 or C2-Flag were generated by lentiviral transduction ...
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bioRxiv - Molecular Biology 2021Quote: ... The two sgRNAs targeting Dazl exon 6 were cloned into the pSpCas9(BB)-2A-GFP backbone (Addgene #48138). The knock-in led to the generation of a reporter cell line named Dazl-mScarlet-HygromycinR (DASH) ...
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bioRxiv - Cell Biology 2021Quote: ... candidate gRNAs were cloned into the pSpCas9(BB)-2A-GFP (PX458) plasmid vector (Addgene plasmid #48138; RRID: Addgene_48138) (Ran et al. ...
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bioRxiv - Cell Biology 2021Quote: ... candidate gRNAs were cloned into the pSpCas9(BB)-2A-GFP (PX458) plasmid vector (Addgene plasmid #48138; RRID: Addgene_48138) (Ran et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138) and was described previously49 ...
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bioRxiv - Neuroscience 2020Quote: ... pSpCas9n(BB)-2A-GFP (PX461) and pX330-U6-Chimeric_BB-CBh-hSpCas9 were a gift from Feng Zhang (Addgene plasmid # 48140 ...
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bioRxiv - Cancer Biology 2022Quote: ... the following sequences were cloned into pSpCas9(BB)- 2A-Puro (PX459) V2.0 (gift from Feng Zhang, Addgene #62988) targeting mouse Col6a1 ...
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bioRxiv - Cell Biology 2022Quote: ... The vector pSpCas9(BB)-2A-Puro (PX459) V2.0 was provided from Feng Zhang (plasmid 62988; Addgene, Cambridge MA) (Ran et al ...
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bioRxiv - Cell Biology 2022Quote: ... annealed oligo (5’-CACCGATGACCAACGACGCAAGTTT-3’ and 5’-AAACAAACTTGCGTCGTTGGTCATC-3’) was inserted into PX459 (pSpCas9(BB)-2A-PuroV2.0) (Addgene) (Ran et al. ...
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bioRxiv - Cell Biology 2020Quote: ... pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid # 48139 ; http://n2t.net/addgene:48139 ; RRID:Addgene_48139)50 The sgRNA oligos (sense ...
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bioRxiv - Systems Biology 2022Quote: pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). The oligonucleotides containing CRISPR guide RNA sequences (sgRNA ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse and human sgRNAs were designed using the CHOPCHOP web tool: (http://chopchop.cbu.uib.no) and cloned into pSpCas9(BB)-2A-Puro (PX459) (Addgene #48139) and lentiCRISPRv2 (Addgene #52961).
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bioRxiv - Cell Biology 2019Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; http://n2t.net/addgene:48138; RRID:Addgene_48138).
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bioRxiv - Cancer Biology 2019Quote: ... was created using pSpCas9(BB)-2A-GFP (a gift from Dr. Feng Zhang, #48138, Addgene, http://n2t.net/addgene:48138 ; RRID:Addgene_48138) as a backbone vector [12] ...
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bioRxiv - Cancer Biology 2019Quote: ... and ligated into the pSpCas9(BB)-2A-EGFP vector (pX458; a gift from Feng Zhang; Addgene Plasmid #48138) at the BbsI cloning site ...
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bioRxiv - Developmental Biology 2019Quote: ... Annealed guides were then cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene, plasmid 62988, PMID: 24157548). Ark -/- MEFs were transfected with 4 guide pairs in order to create the desired deletion using FUGENE (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... The guide sequences were then cloned into vector pX330-U6-Chimeric_BB-CBh-hSpCas9 which was a gift from Feng Zhang (Addgene plasmid # 42230; http://n2t.net/addgene:42230; RRID:Addgene_42230). The T7 promoter was added to the sgRNA template ...