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Citations for Addgene :
501 - 550 of 935 citations for Mouse Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (between passage 9-11) were transfected with lentiviral packaging vector psPAX2 (#12259, Addgene, Watertown, MA, USA), the envelope vector pMD2.G (#12260 ...
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bioRxiv - Cell Biology 2023Quote: ... which was a gift from Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA, USA) (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). GFP-positive cells were sorted after 24 h of transfection and plated ...
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bioRxiv - Cell Biology 2023Quote: ... The lentiviral packaging plasmids pMD2.G and psPAX2 (Didier Trono; 12259, 12260) were obtained from Addgene (Cambridge, MA). Four 15-cm culture plates containing HEK293T cells at 80% confluency were each transfected with 8 μg pTRIPZ-OGT-FH ...
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bioRxiv - Biochemistry 2023Quote: ... and S545A (HS-BG) constructs were cloned into a pHIV-dTomato lentiviral backbone (Plasmid #21374, Addgene, Cambridge, MA) by the Center for Targeted Therapeutics Core Facility at the University of South Carolina (Columbia ...
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bioRxiv - Molecular Biology 2023Quote: ... A recombinant DNA pcDNA3/Myc-DNMT1 was a gift from Arthur Riggs (Addgene plasmid #36939 Watertown, MA, USA) (Li et al. ...
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bioRxiv - Genetics 2023Quote: ... oligonucleotides coding for gRNA sequences were in vitro aligned and cloned into pX330 plasmid (Addgene, Watertown, MA 02472) for co-expression of Cas9 and a corresponding gRNA ...
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bioRxiv - Neuroscience 2023Quote: ... brain slices were cut from DbhCre mice bilaterally injected with AAV1-hSyn1-SIO-stGtACR2-FusionRed (Addgene, MA, USA) into the LC and allowed to recover for 5-8 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA). Virus (0.5 µl ...
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bioRxiv - Plant Biology 2024Quote: 35S:GUS-pMAS:GFP was modified by replacing the 35S promoter with the MAS promoter from pGFPGUSPlus (Plasmid #64401, Addgene) (He et al. ...
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bioRxiv - Synthetic Biology 2023Quote: The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059). CIDAR MoClo Extension ...
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bioRxiv - Biochemistry 2022Quote: ... The PRESTO-Tango plasmid kit was a gift from Bryan Roth (Addgene kit # 1000000068).
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bioRxiv - Synthetic Biology 2023Quote: ... The MoClo-YTK plasmid kit was a gift from John Dueber (Addgene kit # 1000000061). Enzymes used were from New England Biolabs unless stated otherwise ...
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bioRxiv - Molecular Biology 2023Quote: The CRISPaint gene tagging kit was a gift from Veit Hornung (Addgene kit # 1000000086). To generate a targeting construct for AGO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All GPCR plasmids originated from the Roth lab PRESTO-Tango kit (Addgene, Kit #1000000068) and were amplified in E ...
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bioRxiv - Neuroscience 2020Quote: ... Feng Zhang (Addgene kit #1000000019). Once assembled into a destination vector ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids pHSG-PCNA2 and pHSG-PCNA3 were a gift from Teruyuki Nagamune obtained through Addgene (Cambridge, MA, USA) (Addgene plasmid # 66126 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pAR-Ec611 (ArEc-Rev1-611) plasmid harboring medium-error-rate polymerase TP-DNAP1_611 (≥10−6 s.p.b.) was obtained from Addgene (Watertown, MA).
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bioRxiv - Cancer Biology 2020Quote: CRISPR-Cas9-mediated knockout was delivered by pSpCas9 (BB)-2A-GFP (PX458) (Plasmid #48138, Addgene, and Cambridge, MA, USA). Sequences of sgRNAs are listed in the following supplementary table S2 ...
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bioRxiv - Neuroscience 2021Quote: MSCV (Murine Stem Cell Virus)-CreERT2 puro plasmid was a gift from Tyler Jacks (Addgene, Watertown, MA; plasmid # 22776; http://n2t.net/addgene:22776; RRID: Addgene_22776). pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371 ...
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bioRxiv - Cell Biology 2021Quote: ... Sequence analysis and primer design was done in Benchling [68] All of the plasmids were from Addgene (Watertown, MA) and primers (Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well plates and transfected 24h after with pMXs GFP-LC3-RFP (#117413, Addgene, MA) or with Polyinosinic-polycytidylic acid sodium salt ...
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bioRxiv - Genetics 2022Quote: ... Each 20 nt gRNA sequence was each cloned into a PX459 (Ran et al., 2013) (Addgene 62988, Watertown, MA) backbone digested with BbsI (Thermo FD1014) ...
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bioRxiv - Neuroscience 2020Quote: Adeno-associated viruses (AAVs) carrying Cre-inducible (double-inverse orientation; DIO) transgenes were purchased from Addgene (Watertown, MA, USA). We used stimulatory AAV8-hSyn::DIO-hM3Dq-mCherry (4.0e12 GC/ml titer ...
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bioRxiv - Neuroscience 2020Quote: ... We delivered AAV8.hSyn.hM4Di.mCherry (hM4Di) or AAV8.hSyn.mCherry (mCherry) targeting the BLA and contralateral NAc core (Addgene, Watertown, MA) via a micropump (UltraMicroPump III ...
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bioRxiv - Immunology 2021Quote: ... and a separate master mix was prepared comprising 1 mL Opti-MEM 8 µg PSPAX (12260, Addgene, Watertown, MA), 2 µg PMD2G plasmids (12259 ...
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bioRxiv - Neuroscience 2022Quote: ... 200nl of adeno-associated virus 2 (AAV2) containing either control construct (pAAV-hSyn-EGFP; plasmid #50465; Addgene, Watertown, MA) or excitatory DREADD (pAAV-hSyn-hM3D(Gq)-mCherry ...
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bioRxiv - Neuroscience 2020Quote: ... to measure BLA ACh levels (Fig. 2A-E + S2.1-S2.2) or 0.5 µL of AAV1 Syn-FLEX-GCaMP6s-WPRE-SV40 (Addgene, Watertown, MA) to measure BLA principal cell calcium dynamics (Fig ...
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bioRxiv - Microbiology 2020Quote: ... The CD4 expression vector pMX-CD4 and the TR expression vector pMD18-T TR were from Addgene (Watertown, MA) and Sino Biological Inc ...
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bioRxiv - Biochemistry 2020Quote: The MSP1E3D1 plasmid (with a His-tag as well as a TEV protease cleavage site, from Addgene, MA, USA) (26 ...
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bioRxiv - Neuroscience 2021Quote: ... Adeno-associated virus containing the GCaMP7f gene (pGP-AAV9-syn-FLEX-jGCaMP7f-WPRE, 104488-AAV9, Addgene, Watertown, MA, USA) was loaded into a glass micropipette with a tip diameter of 40–50 µm attached to a Nanoject II injection system (Drummond Scientific ...
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bioRxiv - Cell Biology 2021Quote: The ATeam1.03-nD/nA/pcDNA3 plasmid was a gift from Takeharu Nagai (Addgene, Watertown, MA, plasmid # 51958; http://n2t.net/addgene:51958; RRID: Addgene_51958) (Kotera et al. ...
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bioRxiv - Neuroscience 2021Quote: ... AAVrg-CAG-GFP (titer ≥ 7×1012vg/mL, category number 37825, lot V9234) was purchased from Addgene (Watertown, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... This middle entry vector was Gateway recombined using a 5’ entry zebrafish ubiquitious promoter (Addgene #2732, Watertown, MA, USA), a 3’ entry pA vector ...
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bioRxiv - Plant Biology 2022Quote: ... and HlWRKY1 was individually cloned into the bait vector pGBKT7-GW [DNA-binding domain (BD)] (Addgene Inc, MA, USA) to produce fusion proteins containing a GAL4 activation domain (AD ...
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bioRxiv - Neuroscience 2023Quote: ... and pAAV2-hSyn-DIO-EGFP retrograde virus construct (7.6×1012 genome copies/ml; 50457-AAVrg, Addgene, Watertown, MA, USA) was made with a flow rate of 1.0 µl/min ...
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bioRxiv - Molecular Biology 2022Quote: The lentiCRISPR v2 (#52961)43 and lentiCRISPR v2-dCas9 (#112233)44 vectors were purchased from Addgene (Watertown, MA, US).
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bioRxiv - Neuroscience 2023Quote: ... with CRMP1 then being subcloned into pENTR1A no ccDB (20) (Dr. Eric Campeau, AddGene clone 17398, Watertown, MA, USA). Vectors encoding NPAS3 (13 ...
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bioRxiv - Physiology 2023Quote: ... for generation of isogenic control lines were designed using an online tool (crispr.mit.edu) and ordered as oligos to be cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-line(#4954) was transduced using viral particles containing the lentiCas9-Blast vector (Addgene, Watertown, MA; RRID:Addgene_52962). Cells stably expressing Cas9 were selected using 10ug/ml Blasticidin S HCl (Gibco™-#R21001) ...
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bioRxiv - Microbiology 2023Quote: N4BP1 KO Huh7 cells were generated by CRISPR/Cas9 using pX330-U6-Chimeric_BB-CBh-hSpCas9 (pX330, Addgene, MA, USA) and HR110PA-1 (System Biosciences ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Neuroscience 2024Quote: ... A genetically encoded calcium indicator for the detection of ACh activity (1 μL, AAV9-hSyn-ACh3.0; AddGene; Watertown, MA) was infused into the medial prefrontal cortex (AP(2.7mm) ...
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bioRxiv - Neuroscience 2024Quote: ... on p26 subjects underwent a stereotaxic surgical injection of the inhibitory DREADD AAV-CAMKIIa-HM4DGi-mCherry (Addgene, Watertown, MA) to the BLA (A/P ...
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bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... Expression plasmids for WT and catalytic mutant mouse Lipin-1 were obtained from Addgene. Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: The truncated mouse Fzd2 gene (Fzd2-delN) was cloned from pRK5-mFzd2 (#42254, Addgene) with a 1D4 tag at the C terminus by PCR using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... GFP and gRNAs against mouse Ampkα1 (79004) and Ampkα2 (79005) were obtained from Addgene. C2C12 cells were first transfected with Ampkα1 gRNA and sorted with GFP ...
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bioRxiv - Genetics 2022Quote: ... mouse cGAS was amplified via PCR from a pMSCVpuro-eGFP-cGAS template (Addgene, 108675) using primers containing KpnI and NotI restriction sites (S1 Table) ...
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bioRxiv - Cell Biology 2020Quote: Mouse GeCKOv2 CRISPR knockout pooled library was a gift from Feng Zhang (Addgene # 1000000052). To prepare lentivirus particles ...
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bioRxiv - Neuroscience 2023Quote: ... and pCDNA3-mouse PKA-RIIalpha-mEGFP (Addgene plasmid # 45527; http://n2t.net/addgene:45527; RRID:Addgene_45527) were gifts from Haining Zhong 73 ...