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Citations for Addgene :
1 - 50 of 3150 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 Integrase 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: pCDNA3.1 phiC31 integrase (Addgene #68310)20,22 plasmid was linearized with BamHI and transcribed using the T7 mMessage mMachine kit (ThermoFisher AM1344) ...
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bioRxiv - Microbiology 2022Quote: ... psPAX2 (HIV-1 gag/pol) (Addgene) and pMD2.G (encoding VSV-G ...
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bioRxiv - Neuroscience 2023Quote: ... HIV-1 Rev (pRSV-Rev Addgene: 12253), and VSV g-glycoprotein Env (pMD2.G Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... 500–550 nl of AAV9-Syn-jGCaMP7f-WPRE virus (diluted 1:2; Addgene) was injected in dorsal CA1 to express synapsin-driven calcium sensor jGCaMP7f (injection coordinates ...
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bioRxiv - Immunology 2021Quote: ... and HIV-1- gag-pol helper plasmid (pspax2, Addgene) using Lipofectamine 3000 according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... 600 ng of BxbI Integrase Expression Vector (Addgene #51271) and 600 ng of each landing pad donor plasmid were co-transfected ...
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bioRxiv - Microbiology 2021Quote: ... 8 × 105 293 T cells in a 6 cm dish were cotransfected with 2 μg of HIV-1 packaging plasmid pCMVΔ8.2 R (Addgene # 12263); 0.5 μg of the pCMV-VSV-G plasmid (Addgene # 8454 ...
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bioRxiv - Microbiology 2022Quote: ... pDSAP-aapp-hGrx1-roGFP2 and pDSAP-trio-DsRed) (~160 ng/µL) and the integrase helper plasmid carrying the Vasa2-ΦC31-Integrase gene (Addgene # 62299, ~200 ng/µL) diluted in 0.5x phosphate buffered saline (0.1 mM NaHPO4 buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... into the ΦC31 integrase compatible pBID-UASC-G plasmid (Addgene plasmid # 35202 ...
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bioRxiv - Genomics 2022Quote: ... and pLAI-HIV (Addgene #133996)] ...
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bioRxiv - Microbiology 2022Quote: ... HIV-1 GagPol was expressed by pCMV ΔR8.2 (Addgene plasmid # 12263). The protease mutations D25N and R57G were generated by overlapping PCR using pCMV ΔR8.2 as a template ...
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bioRxiv - Genetics 2022Quote: ... The PhiC31 integrase expressing pBS130 plasmid was obtained from Addgene (#26290). The full length of CG5399 was cloned from cDNA of S2R+ cells and inserted into pWalium10 vector (DGRC ...
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bioRxiv - Cancer Biology 2023Quote: ... and a variant of the integrase-deficient psPax-D64 (Addgene #63586). Lipofectamine (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S / HIV-1 pseudotyped viruses were packaged by co-transfecting a lentiviral construct pHIV-Luciferase (Addgene plasmid # 21375), a packaging construct psPAX2 (Addgene plasmid # 12260 ...
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bioRxiv - Cell Biology 2022Quote: ... HIV-1-GAG fused with EGFP was purchased from Addgene (plasmid 80605). Ezrin plasmid was a generous gift of Adam Kwiatkowski (University of Pittsburgh School of Medicine ...
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bioRxiv - Microbiology 2024Quote: The full-length HIV vectors NL4-3 ΔEnv EGFP (HIV Reagent Program) and HIVGKO (Addgene plasmid #112234) were produced in HEK293T cells along with the VSV-G (Addgene plasmid # 8454 ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid pCMV delta R8.2 encoding HIV-1 GagPol (Addgene, plasmid #12263) using Fugene 6 (Promega ...
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bioRxiv - Microbiology 2021Quote: ... and a plasmid pCMV delta R8.2 encoding HIV-1 GagPol (Addgene, plasmid # 12263) using FuGENE 6 (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... packaging plasmid HIV-gag-pol (Addgene 14887) and envelope plasmid pUVSVG (Addgene 8454 ...
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bioRxiv - Neuroscience 2019Quote: ... Virus (1012 titer; AAV2/2 camKII-KV2.1-ChrimsonR-FusionRed; Addgene, plasmid #102771) was injected 400 μm below the dura (4-10 sites ...
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bioRxiv - Neuroscience 2024Quote: Adeno-associated virus (AAV) type 2 carrying cre-dependent ChR2-eYFP (Addgene plasmid 20298 ...
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bioRxiv - Neuroscience 2020Quote: ... we infected adult Brn3cCre mouse retinas with 1 ml Cre dependent AAV Virus (AAV2-CAG-FLEX-EGFP-WPRE, Addgene catalog # ...
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bioRxiv - Neuroscience 2020Quote: One adult mouse (~ 2 months) was stereotaxically injected with a GCaMP6f construct (AAV5.CamKII.GCaMP6f.WPRE.SV40 virus, Addgene # 100834; 0.4 μL at 0.06 μl/min) in hippocampal CA1 ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... and HIV-1 P RBF168) were cloned into the pWPI-GFP vector (Addgene # 12254) to transduce Jurkat cells stably expressing CD4.
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bioRxiv - Cell Biology 2023Quote: ... For retroviral transfections 1 μg VSVG and 1.86 μg HIV gag-pol (Addgene #14887) were used ...
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bioRxiv - Neuroscience 2022Quote: ... 0.7-1 μL of virus (AAV2-CMV-mCreb, 1×1012 Addgene Plasmid #68551 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding phiC31 integrase under control of a heat shock promoter (Addgene #26290) [73]) ...
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bioRxiv - Neuroscience 2023Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding ΦC31 integrase under control of a heat shock promoter (Addgene #26290)60) ...
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bioRxiv - Immunology 2022Quote: ... an HIV gag-pol packaging plasmid (Addgene #8455), and a lentiviral transfer plasmid encoding firefly luciferase (Addgene #170674 ...
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bioRxiv - Immunology 2021Quote: ... an HIV gag-pol packaging plasmid (Addgene #8455), and a lentiviral transfer plasmid encoding firefly luciferase (Addgene #170674 ...
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bioRxiv - Immunology 2024Quote: ... an HIV gag-pol packaging plasmid (Addgene #8455), and a transfer plasmid encoding firefly luciferase (Addgene #170674 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with plasmid pLXSN16E6E7 encoding the human papilloma virus HPVE6E7 gene (Addgene #52394) as described [18] ...
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bioRxiv - Neuroscience 2023Quote: ... and pZac2.1 gfaABC1D-lck-GCaMP6f virus (Addgene virus #52924).
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bioRxiv - Neuroscience 2019Quote: ... (Addgene #60948)69 or pBS130 (encoding phiC31 integrase under control of a heat shock promoter, Addgene #26290)70 ...
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bioRxiv - Neuroscience 2020Quote: ... The virus (Addgene plasmid pAAV-hSyn-Flpo ...
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bioRxiv - Neuroscience 2023Quote: ... USA) and [2] a Cre-dependent virus expressing green fluorescent protein (AAV-Flex-GFP; Addgene) or diphteria toxin (AAV2/9-EF1a-mCherry-Flex-dTA ...
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bioRxiv - Neuroscience 2023Quote: ... we infected organoids for 2 weeks with the AAV1-hSYN-eGFP virus (Addgene, 105539-AAV1), which directs expression of eGFP under the neuron-specific synapsin promoter ...
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bioRxiv - Genomics 2020Quote: Plasmid for HIV-1 reverse transcriptase expression: pCMV-dR8.2 dvpr was a gift from Bob Weinberg (Addgene plasmid # 8455 ...
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bioRxiv - Microbiology 2022Quote: ... pCMV encoding HIV-1 Vpr fused to beta lactamase (pCMV4-BlaM-Vpr) was obtained from Addgene (21950). A plasmid encoding replication-incompetent HIV-1 lacking env and vpr and encoding luciferase (pNL4-3LucR-E- ...
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bioRxiv - Immunology 2022Quote: HIV-based lentivirus backbone plasmid pCMV-dR8.2 dvpr (Addgene #8455), pBOB-Luciferase (Addgene #170674 ...
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bioRxiv - Immunology 2023Quote: ... together with an HIV gag-pol packaging plasmid (Addgene #8455), and a transfer plasmid encoding firefly luciferase (Addgene #170674 ...
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bioRxiv - Bioengineering 2022Quote: ... The phiC31 integrase mRNA was prepared by in vitro transcription using EcoRl-linearized plasmid pCDNA3.1_phiC31 (Addgene #68310) or HindIII-linearized plasmid pT7_PhiC31o as the template and mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs targeting mouse and human genes of interest (see Supplementary Table 2) were cloned into plenti-sgRNA (Addgene plasmid #71409) using BsmbI and into Cas9 plasmid (Addgene plasmid #62988)(Ran et al ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid mixes to produce HIV-1 viral particles consisted of 0.4 μg CMV-VSVG (pMD2.G; 12259; Addgene) and 2.6 μg of HIV proviral plasmid ...
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bioRxiv - Bioengineering 2022Quote: ... into the attP site by co-injecting it with a plasmid expressing integrase from phage PhiC31 (Addgene #183966).
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bioRxiv - Molecular Biology 2023Quote: ... co-transfected with the domain III/pURD construct and an integrase expression vector pgk-φC31/pCB92 (Addgene #18935) [50] ...
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bioRxiv - Neuroscience 2023Quote: ... Virus (AAV1-S5E2-jGCaMP6f; Addgene #135632-AAV1; diluted 1:3 in saline) was injected into the RSC (AP ...
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bioRxiv - Neuroscience 2023Quote: ... a 1 μL dot of AAV9/hSyn/GCaMP6f virus (Addgene, Watertown, MA) diluted 1-5x from commercial titer (2.8x1013 to ∼1x1012 units/mL ...
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bioRxiv - Neuroscience 2020Quote: ... We then injected the AAV5.CamKII.GCaMP6f.WPRE.SV40 virus (Addgene # 100834; 200 nL at 1 nl.s-1) in hippocampal CA1 using the following coordinates ...
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bioRxiv - Neuroscience 2020Quote: ... P5-7 WT C57Bl/6 mice were used for the injection of AAV9 virus under human synapsin promoter (pAAV9.hSyn.iGluSnFR, commercially available from Addgene, USA) to drive transgenic expression of iGluSnFR in SGNs ...