Labshake search
Citations for Addgene :
101 - 150 of 342 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... AAV1-Syn-Chronos-GFP (Chronos, 2.90e+13 gc/mL, 250 nL, Addgene #59170-AAV1), AAV-syn-FLEX-jGCaMP7b-WPRE (FLEX-GCaMP7b ...
-
bioRxiv - Genetics 2024Quote: ... We next used the Gateway™ LR Clonase™ II Enzyme mix to recombine each of the four PCR products into the pBID-UASC-G backbone (Addgene Plasmid #35202), which contains a φC31 integrase compatible attB sequence and UAS binding sites for the GAL4 expression system (Wang et al ...
-
bioRxiv - Neuroscience 2019Quote: ... All viruses were used at a titer of 1012 GC/mL and acquired from Addgene.
-
bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-DIO-hChR2(H134R)-EYFP (Addgene, 1.8*10^13 gc/ml, diluted 1:10), AAV2-hSyn-hChR2(H134R)-EYFP (UNC Vector Core ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV encoding AAV5- CBA-FLEX-ArchT-tdTomato (Titer: 1.3 × 1013 GC/mL) (#28305; Addgene, USA) after 10-fold dilution with PBS was used ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV vectors containing Cre-inducible GCaMP6f (AAV.9.Syn.Flex.GCaMP6f.WPRE.SV40, Addgene; TIter≥2.1×13 GC/ml) were injected into the right DLS or DMS by stereotaxic surgery ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV2/1-DIO-CAG-GCaMP6f was purchased from Addgene (# 100839, titer: 1.4 × 1013 gc/ml). To chemogenetically activate PVNOT neurons by means of DREADD we employed AAV2/1-DIO-hSYN1-hM3Dq-mCherry (Addgene # 44361 ...
-
bioRxiv - Neuroscience 2023Quote: ... Retro-AAV2 coding for mCherry (pAAV2-hSyn-mCherry, Addgene #114472, 2 x 1013 GC/ml) and green fluorescent protein (pAAV2-hSyn-eGFP ...
-
bioRxiv - Neuroscience 2023Quote: ... Janelia) or AAVretro_Syn_GCamp7f (1.0e13 GC/ml, lot #21720; 1.2e12GC/ml, lot #v52598, Addgene 104488-AAVrg) in the right dorsolateral striatum (DLS ...
-
bioRxiv - Neuroscience 2023Quote: ... into the VP and retro-AAV Cre (0.4uL, titer ∼ 1.03 × 1014 GC/mL, Addgene #51507,) into the ipsilateral VTA at a rate of 0.1uL/min ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV1-Syn-GCaMP6f-WPRE-SV40 (titer: 2.8 × 10^13 GC/mL) was purchased from AddGene and was diluted 1:4 in sterile 1× PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... we injected AAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (5×1011 gc/mL; Addgene) bilaterally into the lateral hypothalamus of the previously characterized and validated MCH-Cre mice42 ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV5-Syn-PSAM4-GlyR-IRES-eGFP (2.4 × 10^13 GC/mL) was purchased from AddGene. Mice had the virus injected at stock titer bilaterally into dorsal and ventral hippocampus ...
-
bioRxiv - Neuroscience 2024Quote: ... plus either AAV8-hSyn-DIO-hM4Di-mCherry (2.2 x 1013 GC/mL, Addgene, Watertown, MA) or AAV8-hSyn-DIO-mCherry (Control virus ...
-
bioRxiv - Neuroscience 2024Quote: ... AAV-syn-FLEX-jGCaMP7b-WPRE (FLEX-GCaMP7b, 1.90e+13 gc/mL, 250nl, Addgene #104493-AAV1), ssAAV-retro/2-hSyn1-mCherry_iCre-WPRE-hGHp(A ...
-
bioRxiv - Neuroscience 2023Quote: ... injected unilaterally into the VP and retro-AAV Cre (titer ∼ 1.1 × 1013 GC/mL, Addgene #51507) injected into either the ipsilateral VTA (0.4uL ...
-
bioRxiv - Neuroscience 2023Quote: ... For anterograde transsynaptic tracing: AAV1-hSyn-Cre-WPRE-hGH (1013 gc/ml, Addgene; 1:10 dilution) was injected in DCN (coordinates as above) ...
-
bioRxiv - Neuroscience 2023Quote: ... They were injected retro-orbitally with AAV-PHP.eB-CAG-DIO-GCaMP7f (2.46×1013 GC/mL, Addgene) at postnatal days 14 for as they failed to breed with Ai148D mice ...
-
bioRxiv - Neuroscience 2023Quote: ... UNC Vector Core) or rAAV5-hSyn-DIO-hM3D(Gq)-mCherry (4.2*10^12 gc/ml; Addgene) in the Locus Coeruleus (LC ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV8-hSyn-DIO-mCherry (3.6x1012 gc/ml) was a gift from Bryan Roth (Addgene plasmid # 50459); AAV8-pEF1a-DIO-FLPo-WPRE-hGHpA (2x1012 gc/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... neurons were transduced with AAV9-Syn (5 x 109 gc per well) encoding jGCaMP8s (AddGene 162374) or CaBLAM (in house prep) ...
-
bioRxiv - Neuroscience 2024Quote: ... AAVrg-hSyn-Cre-WPRE-hGH (retro-Cre, 2.10e+13 gc/mL, 80 nL, Addgene #105553-AAVrg), AAV1-Syn-Chronos-GFP (Chronos ...
-
bioRxiv - Microbiology 2020Quote: ... pBluescript II KS(+) (Addgene) was utilized ...
-
bioRxiv - Neuroscience 2021Quote: ... in combination with 150nL of retrograde AAV-Cre-EBFP (Titer: 1×1013 GC/mL, Lot #V15413, Addgene) injection in PF ...
-
bioRxiv - Neuroscience 2021Quote: ... was loaded with the GCaMP6s virus (AAV9.Syn1.GCaMP6s.WPRE.SV40, ≈1.9x1013 GC/ml, Penn Vector Core; RRID: Addgene_100843) and was slowly lowered into VTA (DV -8.1 mm relative to brain surface) ...
-
bioRxiv - Neuroscience 2022Quote: ... consisting of AAV1-Syn1(S)-FLEX-tdTomato-T2A-SypEGFP (1.8 × 1013 GC/ml, 133 nl, Addgene #51509) and AAV9.CaMKII 0.4.Cre (2.1 × 1013 GC/ml ...
-
bioRxiv - Neuroscience 2020Quote: ... A cohort of SOM.iPKR and PKCδ.iPKR mice were injected with bilaterally in CeL with 200 nl of AAV.Eef1a1 Pr.DIO.EGFPL10a (7 × 10^^12 GC/ml, Addgene) for immunohistochemistry experiment; ...
-
bioRxiv - Neuroscience 2021Quote: ... and contralaterally in VP with a matched AAV2 DIO-mCherry control vector (4.7 × 1012 GC/mL, AddGene). Three weeks later ...
-
bioRxiv - Neuroscience 2022Quote: Rbp4-cre mice were bilaterally injected with AAV5-hSyn-hM4D(Gi)-mCherry (1.2 × 10e13 GC/ml, Addgene) into POm (AP -2.0 ...
-
bioRxiv - Neuroscience 2022Quote: ... versus a respective control virus AAV2/1-DIO-hSYN1-mCherry (Addgene # 50459; titer: 9 × 1012 gc/ml). To ablate PVNOT neurons ...
-
bioRxiv - Neuroscience 2022Quote: ... pENN-AAV1-hSyn-Cre-WPRE (titer: 1.8×10e13 GC/ml, Addgene 105553, gift from James M. Wilson) was injected into the piriform cortex or medial prefrontal cortex ...
-
bioRxiv - Neuroscience 2022Quote: ... in the same pipette a combination of AAV2-pCAG-FLEX-eGFP- WPRE (2.5E12 GC/mL, Addgene 51502) and flp-dependent mCherry (2E13 GC/mL ...
-
bioRxiv - Neuroscience 2023Quote: ... 400 nl of AAV2/5-CAG-dLight1.1 (1.7×1013 GC/ml, 111067-AAV5, Addgene, Watertown, MA, USA) was slowly injected into the DMS (n = 7 ...
-
bioRxiv - Neuroscience 2023Quote: ... and 150-250 nL of AAV5-EF1α-DIO-hChR2(H134R)-mCherry (5.25 x 1012 GC/ml, RRID:Addgene_20297), AAV5-EF1α-DIO-mCherry (7.3 x 1012 GC/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... we injected AAV9-CaMKIIa.C1V1(t/t).TS.EYFP (titer: 2.3 × 1013 GC/ml; prepared by Addgene, MA, USA) into the unilateral dorsal hippocampus as described previously 87.
-
bioRxiv - Neuroscience 2023Quote: ... A control vector lacking the hM4Di receptor was also used (AAV8-CaMKII-EGFP; 2.1 x 1013 gc/ml Addgene viral prep # 50469-AAV8; http://n2t.net/addgene:50469; RRID:Addgene_50469 ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV2/2-hsyn-DIO-hM4D(Gi)-mCherry was obtained from Addgene (plasmid #44362, 4.6×1012 GC/ml). For optogenetic activation ...
-
bioRxiv - Neuroscience 2023Quote: ... a kind gift from Don Arnold) and pENN.AAV.CamKII 0.4.Cre.SV40 (viral titer/ml: 2.3 x 10^13 GC/ml (Addgene 105558-AAV9) in the ratio 2:1:1 ...
-
bioRxiv - Neuroscience 2024Quote: ... Cre-dependent control plasmid pAAV-hSyn-DIO-mCherry (AddGene #50459-AAV8; titer of 2.2×1013 GC/mL), or the retrogradely transported Cre-expressing plasmid pENN-rAAV-hSyn-HI-eGFP-Cre-WPRE-SV40 #105540-AAVrg ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV vector encoding for inhibitory DREADD (AAV5-SYN1-hM4Di-HA 1.7*1013 GC/ml (Addgene, Watertown MA)) was injected into the bilateral basolateral amygdala stereotaxically ...
-
bioRxiv - Neuroscience 2024Quote: ... AAV5-Syn-FLEX-rc [ChrimsonR-tdTomato] (FLEX-Chrimson, 1.20e+13 gc/mL, 250 nL, Addgene #62723-AAV5), AAVrg-hSyn-Cre-WPRE-hGH (retro-Cre ...
-
bioRxiv - Neuroscience 2021Quote: Mice were injected with 100nL of retrograde AAV-Syn-eGFP (Titer: 1×1013 GC/mL, Lot#V16600, Addgene) in PF (N=6) ...
-
bioRxiv - Neuroscience 2021Quote: Mice were injected with inhibitory DREADDs pAAV8-hSyn-DIO-hM4DimCherry (Titer: 2.9×1013 GC/mL, Vol: 100μL, Lot: v54499; Addgene) in IN ...
-
bioRxiv - Neuroscience 2019Quote: ... 120 to 150 nl of AAV1.hSynap.SF-iGluSnFR.A184S.WPRE.SV40 (HHMI Janelia Research Campus and Addgene, titer 2.7e13 GC/ml) were pressure-injected at a depth of approximately 350 μm ...
-
bioRxiv - Neuroscience 2021Quote: ... Subset of pups were bilaterally injected with 4 μl AAV9-hsyn-EGFP (3.4×10^13 gc/ml, Addgene) or 4 μl ACh3.0 (1.8×10^13 gc/ml ...
-
Basolateral amygdala parvalbumin neurons report aversive prediction error to constrain fear learningbioRxiv - Neuroscience 2020Quote: ... AAV5-ef1α-DIO-eNpHR3.0-eYFP (1.1×1013 GC/ml) (pAAV-Ef1a-DIO eNpHR 3.0-EYFP was a gift from Karl Deisseroth (Addgene viral prep # 26966-AAV5 ...
-
bioRxiv - Neuroscience 2020Quote: ... 10 animals received rejections of AAV5-CaMKIIa-hM4Di-mCherry (titer 9.5×10^12 GC/ml; Addgene, MA, USA), while eight animals received injections of a non-DREADD expressing viral control AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml ...
-
Independent representations of reward-predicting cues and reward history in frontal cortical neuronsbioRxiv - Neuroscience 2020Quote: ... The AAV solution (AAV1-hSyn-NES-jRGECO1a, titer ~1 × 1013 GC/ml, the viral solution provided by Addgene, viral prep # 100854-AAV1 ...
-
bioRxiv - Neuroscience 2021Quote: ... The visual cortex was injected with a total of 2–3 μl of virus solution (1e13 GC/ml) containing AAV9-Syn-GCaMP6s.WPRE.SV40 (Penn Vector Core or Addgene) through a beveled glass micropipette (tip size 10–20 μm diameter ...
-
bioRxiv - Neuroscience 2021Quote: ... heterozygous D2-Cre mice were bilaterally injected with 400 nl of AAV5-CAG-FLEX-ArchT3.0-tdTomato (1.3×1013 GC/ml, Addgene) or AAV5-EF1a-DIO-eYFP (1.3×1013 GC/ml ...