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Citations for Addgene :
151 - 191 of 191 citations for Chromosome 11 Open Reading Frame 53 C11ORF53 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... in mouse G4 ES cells the HA-NLS-FlpO-WPRE-Sv40-pA-LoxP-PGK-Neo-polyA-LoxP cassette in frame with the ATG of the endogenous Apln gene (full plasmid sequence and DNA to be deposited at Addgene # reference). All primer sequences required to genotype this mice are provided in Extended Data Table 1.
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bioRxiv - Cell Biology 2020Quote: ... with oligos 5’-CCCaagcttACCATGGACTATAAGGACGATGATGACAAAGAACACCAGCTCC-3’ and 5’-CCCaagcttCTCCGGATTACCTTCATCCTTATGTAGATAAGAGTGCTGCAG-3’ and cloned into the HindIII site in frame upstream of pcDNA5 FRT/TO 3xHA-Venus-1xminAID (Daniel et al., 2018) (identical to Addgene #117714) but with a neomycin instead of hygromycin resistance ...
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bioRxiv - Developmental Biology 2020Quote: ... in-frame with a codon-optimized Cre sequence (Ohtsuka et al., 2013; Shimshek et al., 2002) designed from pBBI (Addgene #6595). These were flanked on the 5’ and 3’ sides with 93 bases and 95 bases ...
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bioRxiv - Neuroscience 2023Quote: ... Male PV-Cre and both male and female Ai6 mice were secured in a stereotaxic frame and bilateral injections of 200-300 nl AAV1 virus vectors (AAV1-EF1a-DIO-ChETA-eYFP (Addgene: 26968) to PV-Cre mice and pENN-AAV1-hSyn-Cre-WPRE-hGH (Addgene ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... The inserts of the pENTR™11 constructs were transferred into the lentiviral expression vector pInducer20 (Addgene, 44012) using Gateway cloning (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (between passage 9-11) were transfected with lentiviral packaging vector psPAX2 (#12259, Addgene, Watertown, MA, USA), the envelope vector pMD2.G (#12260 ...
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bioRxiv - Biophysics 2022Quote: Escherichia coli NiCo21 (DE3) competent cells were transformed with the plasmid encoding sumo-tag fused DiCas7-11 (Addgene: 172503). A single colony was picked and transferred to 100mL LB media containing 50 μg/mL ampicillin and grown for 12 hours at 37°C before inoculation into 1L LB culture ...
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bioRxiv - Neuroscience 2023Quote: ... Rats intended for fiber photometry experiments (n=11 females) received unilateral infusions of AAV9 FLEX-GCaMP6f (0.4uL, titer ∼1.4 × 1013 GC/mL, Addgene #100833) into the VP and retro-AAV Cre (0.4uL ...
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bioRxiv - Molecular Biology 2023Quote: pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #186981 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the U6-crRNA scaffold and the DiCas7-11 gene sequence from pDF0114 (Addgene #172508) and pDF0159 (Addgene #172507) ...
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bioRxiv - Genomics 2020Quote: THP-1 and MV4;11 cells were engineered to stably express humanized S.pyogenes Cas9 endonuclease by lentiviral transduction with the FUCas9Cherry vector (Addgene 70182) and subsequent FACS-selection for mCherry-positive cells (THP-1-Cas9 ...
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bioRxiv - Bioengineering 2023Quote: ... Human codon optimized DisCas7-11 protein sequence and the mature DR guide scaffold with golden gate sites were PCR amplified from Addgene plasmids # 172507 and #172508 ...
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bioRxiv - Neuroscience 2023Quote: Th-cre rats were randomly assigned to a viral group and infused bilaterally with a cre-dependent AAV encoding either the inhibitory opsin archaerhodopsin T (ArchT; N = 11; 6 males; AAV5-CAG-FLEX-ArchT-tdTomato, Addgene) or a tdTomato fluorescent protein control (tdTomato ...
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bioRxiv - Physiology 2024Quote: ... pLenti PGK Puro vectors expressing stable-HIF-11 (Plasmid #177202, addgene), pMD2.G (Plasmid #12259, addgene) and psPAX2 (Plasmid #12260, addgene) were ordered from Addgene.
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bioRxiv - Microbiology 2024Quote: ... wells were transfected with 500 ng of RSV Gag-GFP (11) and 125 ng of FLAG-tagged Med26 (a gift from Joan Conaway and Ronald Conaway [Addgene plasmid #15367 ...
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bioRxiv - Molecular Biology 2019Quote: ... A lentivirus construct containing the biotin ligase BirA was generated from the lentiCRISPR v2 backbone (Addgene 52961)[61] and a construct containing BirA (a gift from Mauro Modesti ...
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bioRxiv - Molecular Biology 2019Quote: ... Retroviral vectors for JMJD6 expression were generated by cloning the Jmjd6 coding sequence with a C-terminal FLAG-tag sequence (11) into the pBABE plasmid vector containing the neomycin resistance gene (Addgene #1767). Mutant versions of JMJD6 were generated from the wild type construct using QuikChange Lightning site-directed mutagenesis kit (Agilent) ...
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bioRxiv - Biophysics 2021Quote: ... were transfected in DMEM with 2 % (v/v) FBS with 12 μg of pHR-CMV-TetO2 construct vector alongside 11 μg envelope plasmid (pMD2.G; Addgene #12259) and 11 μg packaging plasmid (psPAX2 ...
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bioRxiv - Cell Biology 2020Quote: ... Primers containing this targeting sequence (primers 11 and 12) were annealed and subcloned into the pU6-(BbsI) CBh-Cas9-T2A-mCherry vector (Addgene #64324) digested with BbsI.
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bioRxiv - Neuroscience 2023Quote: ... The pSAD 11.G H2B:GFP was obtained by taking advantage of the pSAD 11.G F3 expression vector (kindly provided by M. Tripodi, Cambridge University, AddGene #32634). The nucleotide sequence encoding the histone H2B-tagged GFP was designed and ordered via GeneArts (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers 11 bearing terminal NotI restriction sites were cloned into PCR4-Shh::lacZ-H11 (Addgene, # 139098). Plasmid clones were validated by Sanger sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... pWC223 encoding the motor domain of Drosophila kinesin 1 fused to BCCP (noted Kin1-biotin) was a gift from Jeff Gelles (Addgene plasmid # 15960; http://n2t.net/addgene:15960; RRID:Addgene_15960).
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bioRxiv - Cell Biology 2019Quote: ... The K18-Scarlet-I reporter was constructed by cloning a fusion of the K18 construct from pMK1253 in frame with the C-terminal mScarlet-I (Addgene #98839, a gift from Dorus Gadella (65)) as well as replacing the EF1a promoter with a CAG promoter to obtain pJC49.
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bioRxiv - Neuroscience 2022Quote: ... pAAV-EF1α-DIO-GCaMP6m-WPRE-pA or pAAV-GfaABC1D-EGFP-WPRE-pA) with pAAV-RC2/11 (or pAAV-RC2-retro or pAAV-RC9) and pAdDeltaF6 (Addgene plasmid #112867) were co-transfected into HEK-293T cells at the molecular ratio of 1:1:1 ...
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bioRxiv - Systems Biology 2022Quote: ... The nuclear marker H2B-miRFP703 is a fusion of the human H2B clustered histone 11 (H2BC11) with the monomeric near-infrared fluorescent protein miRFP703 (Shcherbakova et al. 2016) (Addgene plasmid #80001). ERK-KTR-mRuby2 ...
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bioRxiv - Molecular Biology 2023Quote: ... the ORFs were cloned into plasmid H6-mCerulean (pET Biotin His6 TEV mCerulean LIC cloning vector, Addgene plasmid #29726). In the second step ...
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bioRxiv - Immunology 2023Quote: Expi293F cells were cultured and transiently transfected with the pLenti-palmGRET reporter plasmid encoding the dual reporter palmitoylated EGFP-nanoluciferase protein (PalmGRET) (Addgene plasmid #158221 11) for EV production as described previously 23 ...
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bioRxiv - Molecular Biology 2019Quote: ... 293T cells were cotransfected with vectors encoding an RNA bait flanked by BoxB stem loops and the BASU biotin ligase (Addgene #107253 and #107250 ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplified PCR products were fused to biotin ligases via In-Fusion Recombination into myc-BioID2 pBabe (Addgene #80900; XhoI/PmeI), BioID2-HA pBabe (Addgene #120308 ...
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bioRxiv - Cell Biology 2020Quote: ... pWC223 encoding the motor domain of Drosophila kinesin 1 fused to BCCP (noted Kin1-biotin) was a gift from Jeff Gelles (Addgene plasmid # 15960 ...
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bioRxiv - Plant Biology 2020Quote: ... These enhancers were inserted into the SacI, XbaI, XhoI, and SfoI sites of the plasmid pZS*11-yfp0 (Subramaniam et al., 2013) (Addgene #53241, https://www.addgene.org/53241/). The resulting plasmid (pZS*11_4enh ...
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bioRxiv - Biophysics 2022Quote: ... and pET21a-BirA (Biotin Ligase) was a gift from Alice Ting (Addgene plasmid # 20857; http://n2t.net/addgene:20857; RRID:Addgene_20857, Howarth et al., 2005).
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bioRxiv - Molecular Biology 2023Quote: ... were fluorescently tagged in two steps by first cloning the ORFs into plasmid H6-mOrange (pET Biotin His6 mOrange LIC cloning vector, Addgene plasmid #29723) and then into plasmid 438B.
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bioRxiv - Cell Biology 2023Quote: ... Plasmids expressing murine PKA (mPKA) RIIα and ER membrane targeting signal fused miniTurbo promiscuous biotin ligase (ER-mTb) were obtained from Addgene (#45527 and #107174, respectively). The mPKA RIIα sequence was PCR amplified and cloned into the upstream of the mTb sequence ...
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bioRxiv - Cell Biology 2021Quote: ... Single-chain antibodies fused to GFP (scAB-GFP, Addgene #104998) were used for imaging translation sites through nascent polypeptide labeling ...
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bioRxiv - Immunology 2024Quote: ... VDJ sequences were ordered from IDT and cloned into the vector AbVec antibodies (Addgene)
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bioRxiv - Bioengineering 2020Quote: ... Heavy and light chain DNA sequences of antibody fragments (Fab) were purchased from Twist Bioscience and cloned separately into the pHLsec mammalian expression vector (Addgene, #99845) via Gibson assembly ...
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bioRxiv - Microbiology 2021Quote: ... the antibody component of the SunTag system (pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS45, a gift from Ron Vale, Addgene #60904) was digested with EcoRI+NotI and subcloned into the blunted BamHi site of pCW-TRE ...
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bioRxiv - Bioengineering 2023Quote: ... To generate the monoclonal IgG1 antibody CSL362 biosimilar MIRG123 the VH and VKL sequences were cloned into AbVec2.0-IGHG1 (Addgene plasmid # 80795) and AbVec1.1-IGKC (Addgene plasmid # 80796) ...
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bioRxiv - Cell Biology 2020Quote: The ATF4-SunTag reporter was stably integrated into a previously-described HeLa-11ht cell line stably expressing GFP-tagged single-chain antibodies (scFvGFP) against GCN4 (Addgene plasmid #104998) and NLS-stdMCP-stdHalo fusion proteins (Addgene plasmid #104999 ...
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bioRxiv - Genetics 2024Quote: To generate the B16F10 Capza3 KO lines the 2 CRISPR gRNAs enriched in the radiation alone and radiation and anti-PD1 antibody screen treatment conditions were cloned into the LRG vector (Addgene Plasmid # 65656). For WT controls a NTC gRNA from the CRISPR screen was cloned into the LRG vector ...