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Citations for Addgene :
401 - 450 of 686 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... pcDNA3-HA-RIPK3 (78804) and pcDNA3-FLAG-RIPK1 (78842) were procured from Addgene.
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... A viral vector containing a Cre expressing cassette (pAAV-CMV-HI-eGFP-Cre-WPRE-SV40, Addgene; #105545) was used to induce Fkbp5 deletion in Fkbp5lox/lox mice ...
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bioRxiv - Biophysics 2020Quote: ... Two plasmids containing full-length untagged Npl4 and C-terminal His-tagged Ufd1 were purchased from Addgene. Npl4 fragments were expressed in pET-28a vector with an N-terminal His-SUMO tag ...
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bioRxiv - Biochemistry 2020Quote: hnRNPA2 LC (190-341), insoluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98657)
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bioRxiv - Biochemistry 2022Quote: AR-LBD (663-919) containing an N-terminal His-tag and encoded in pET15b plasmid (Addgene #89083) was expressed in Rosetta (DE3 ...
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bioRxiv - Cancer Biology 2021Quote: ... we cloned Myc-FLAG tagged POT1 into T7 expression vector (pcDNA 5/FRT, Addgene) and performed site-directed mutagenesis ...
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bioRxiv - Cell Biology 2022Quote: pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A was a gift from Nicolas Manel (Addgene plasmid # 86674 ...
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bioRxiv - Cell Biology 2019Quote: ... pFRT/FLAG/HA-DEST QKI was a gift from Thomas Tuschl (Addgene plasmid #19891)56 ...
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bioRxiv - Molecular Biology 2019Quote: ... RACK1-FLAG was cloned into pLenti CMV Puro DEST (w118-1) (Addgene plasmid #17452) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and pBa-EGFP-flag-BicD2594-FKBP (Addgene plasmid #63569; http://n2t.net/addgene:63569; RRID:Addgene_63569), pBa-FRB-3myc-KIF13A tail 361- 1749 (Addgene plasmid #64288 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-mNep2-FLAG (mNeptune2-N1, a gift from Michael Davidson (Addgene plasmid # 54837 ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids ordered were HDAC2 Flag (Addgene plasmid # 36829; http://n2t.net/addgene:36829; RRID:Addgene_36829), HDAC3 Flag (Addgene plasmid # 13819 ...
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bioRxiv - Cell Biology 2020Quote: ... or FLAG-MKL1 or S449A/T450A/S454A (Addgene plasmid 19845, Muehlich et al., 2008). Phenotypic analyses of siRNA treated cells were at 48-72 h post initial siRNA treatment ...
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bioRxiv - Neuroscience 2019Quote: ... astrocytes were transfected with 2 μg of pLYS1-FLAG-MitoGFP-HA (Addgene plasmid # 50057) which contains the pore-forming subunit of the mitochondrial calcium uniporter coupled to GFP or a mito-mCherry construct generated by subcloning the targeting sequence of the pLYS1-FLAG-MitoGFP-HA plasmid into the mcherry2-N1 vector (Addgene plasmid # 54517) ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... and sub-cloned into XbaI/EcoRI sites of Flag-HA-pcDNA3.1 vector (Addgene #52535). Fragments of Dnmt1 (2–290 ...
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bioRxiv - Cancer Biology 2021Quote: ... pRK5-TGFβRI-FLAG plasmid (#14833) and pCMV5B- TGFβRII plasmid (#11766) were purchased from Addgene.
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 mRNA was transcribed in vitro from pST1374-NLS-flag-linker-Cas9 (Addgene, 44758). Then ...
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bioRxiv - Immunology 2020Quote: ... pTRIP-CMV-tagRFP-FLAG-cGAS was a gift from Nicolas Manel (Addgene plasmid # 86676); pCW57.1 was a gift from David Root (Addgene plasmid # 41393) ...
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bioRxiv - Cell Biology 2021Quote: ... Either a control vector (c-Flag pcDNA3 was a gift from Stephen Smale (Addgene plasmid #20011 ...
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bioRxiv - Microbiology 2023Quote: ... The HMGB1 sequence was obtained from the pcDNA3.1 Flag hHMGB1 plasmid (Addgene plasmid #31609).
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bioRxiv - Cancer Biology 2023Quote: ... pCDNA5-FLAG-BRD4-WT and pFLAG-CMV2-BRD4 ΔET plasmids were purchased from Addgene. pCDNA-Flag vector control plasmid was a kind gift from Dr ...
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bioRxiv - Developmental Biology 2023Quote: NPCs were transfected with a FLAG-YAP1 plasmid (Addgene #27371, (Cappello et al., 2013)) and a control plasmid (pEGFP-C1 plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... and pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS (Gift from Roberto Zoncu (Addgene #134653)) in a Clontech vector ...
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bioRxiv - Cell Biology 2023Quote: ... The pLJM1-FLAG-HA-AGO2-WT (Addgene plasmid # 91978; http://n2t.net/addgene:91978; RRID:Addgene_91978) and pLJM1-Empty (Addgene plasmid # 91980 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids pLJM1-FLAG-GFP-OSBP was from Addgene (Plasmid #134659; (Lim et al., 2019)) ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-OSBP was created by amplifying OSBP from pLJM1-FLAG-GFP-OSBP (Addgene #134659) and inserting it by Gibson Assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The MSCV-CMV-CMV-Flag-HA-JMJD6 was purchased from Addgene (Addgene # plasmid 31358). The retrovirus packaging was done as described in the following procedure ...
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bioRxiv - Immunology 2021Quote: ... gene sequence was subcloned from pcDNA5/FRT/TO HIS HSPA1A (a gift from Harm Kampinga, Addgene plasmid # 19537; http://n2t.net/addgene:19537; RRID:Addgene_19537)(46 ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008; http://n2t.net/addgene:79008; RRID:Addgene_79008) (D ...
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bioRxiv - Neuroscience 2020Quote: ... pEZYmyc-His (Addgene plasmid #18701; http://n2t.net/addgene:18702; RRID: Addgene_18701; a gift from Yu-Zhu Zhang [72]), that was digested with the same enzymes ...
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bioRxiv - Biochemistry 2022Quote: Codon optimized plasmids encoding the individual His-tagged PBRM1 bromodomain constructs were received from Nicola Burgess-Brown (Addgene plasmid numbers 38999 ...
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bioRxiv - Biophysics 2022Quote: ... GST-mCherry-FYVE (https://www.protocols.io/view/expression-and-purification-protocol-of-gst-mch-fy-b8k5ruy6) and His-TEV-ATG3 (Addgene_169079 ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid pET28HIS-hAPE1 (for WT His-APE1 protein) was a gift from Primo Schaer (Addgene plasmid #70757; http://n2t.net/addgene:70757; RRID:Addgene_70757) (69) ...
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bioRxiv - Biochemistry 2023Quote: The construct for E.coli expression of WT caspase-3 (pET23b-Casp3-His) was obtained from Addgene (plasmid 11821). The construct for mammalian expression of caspase-3/GFP was a gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: ... The construct for LbCas12a expression (pMAL-His-LbCpf1-EC) – a gift from Jin-Soo Kim (RRID: Addgene 79008) (Kim et al. ...
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bioRxiv - Microbiology 2021Quote: ... pRSET his-eGFP [76] was used as a backbone and was a gift from Jeanne Stachowiak (Addgene plasmid # 113551). Wild-type IDR was PCR amplified from a full-length PEMV2 infectious clone ...
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bioRxiv - Neuroscience 2021Quote: ... and retrograde AAV encoding Cre recombinase (AAVretro-hSyn-HI-eGFP-Cre (titer 1.17 x 1013 GC/ml, Addgene 105540) were stereotaxically injected into S2 or M1 and nwS1 ...
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bioRxiv - Bioengineering 2020Quote: Plasmid pBAD-His-6-Sumo-TEV-LIC cloning vector (8S) was a gift from Scott Gradia (Addgene plasmid 37507). The plasmid was modified via restriction enzyme digestion (final construct ...
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bioRxiv - Cell Biology 2020Quote: ... Histidine-tagged ubiquitin (pCI-His-hUbi, #31815) and Ha-tagged p62 (HA-p62, #28027) plasmids were purchased from Addgene. DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen ...
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bioRxiv - Biochemistry 2022Quote: ... with an initial Met and a cleavable C- terminal TEV 6x-His tag was a gift from Ginkgo Bioworks (Addgene plasmid 145611; http://n2t.net/ addgene:145611; RRID: Addgene_145611). pGBWm4046852 (coding for full- length nsp8 ...
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bioRxiv - Biochemistry 2022Quote: ... with an initial Met and a cleavable C-terminal TEV 6x-His tag) was a gift from Ginkgo Bioworks (Addgene plasmid 145584; http://n2t.net/ addgene:145584; RRID: Addgene_145584). The pET-28a-nsp9 gene was obtained from BEI Resources (NR-53501) ...
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bioRxiv - Biophysics 2019Quote: ... This α-actinin1-SNAP-His fragment was introduced downstream of the T7 promoter of pET T7-7 plasmid (Addgene).
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bioRxiv - Biochemistry 2020Quote: The MSP1E3D1 plasmid (with a His-tag as well as a TEV protease cleavage site, from Addgene, MA, USA) (26 ...
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bioRxiv - Cell Biology 2023Quote: ... Mis18BP120-130 was cloned in pEC-K-3C-His-GST and pET His6 MBP TEV (9C Addgene plasmid #48286).
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bioRxiv - Cell Biology 2023Quote: ... we cloned human NDP52 cDNA in a pETDuet-1 vector with an N-terminal 6×His tag followed by a TEV cleavage site (RRID:Addgene_187829). After the transformation of the pETDuet-1 vector encoding 6×His-TEV-mCherry-NDP52 in E ...
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bioRxiv - Cell Biology 2023Quote: ... we cloned human OPTN cDNA in a pETDuet-1 vector with an N-terminal 6×His tag followed by a TEV cleavage site (RRID:Addgene_190191). After the transformation of the pETDuet-1 vector encoding 6×His-TEV-mCherry-OPTN in E ...
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bioRxiv - Neuroscience 2024Quote: ... the CaMPARI2 gene (1446 bp) was subcloned from pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (Addgene 101060, gift from Eric Schrieter)26 into a lentiviral plasmid (pRT050 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV injections consisted of a 1:10 cocktail of Cre [AAV5-CMV-HI-eGFP-Cre.WPRE.SV40 (Addgene plasmid no. 105545) packaged at UPenn Vector Core 2.5 × 1014 GC ml−1] and KORD [AAV8-HSyn-DIO-HA-KORD-IRES-mCitrine (Addgene plasmid no ...
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bioRxiv - Cell Biology 2020Quote: ... The pSIN-3×flag-ATF3 vector was derived from pSin-EF2-Nanog-Pur (#16578, Addgene). HEK293T cells were seeded so that cells density could be 80% confluence when it comes to transiently transfection ...
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bioRxiv - Immunology 2019Quote: ... and pcDNA3-N-FLAG-Asc (a gift from Bruce Beutler, #75134, Addgene, Watertown, MA, USA) as templates ...