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Citations for Addgene :
451 - 500 of 686 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... full length DVL2 and DVL3 PCR-fragments were amplified from 3X-FLAG-DVL2 (Addgene #24802) and XE251-pcDNA3.1 (zeo ...
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bioRxiv - Cell Biology 2021Quote: ... and pCMV3 nCoV2019 Spike (D614 variant) C-FLAG Hygro was obtained from Addgene (Teddington, UK). Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs ...
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bioRxiv - Cancer Biology 2021Quote: ... or RUNX1ΔEx6 were ligated into XhoI/EcoRI restriction sites in the c-Flag pcDNA3vector (Addgene plasmid #20011 ...
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bioRxiv - Immunology 2022Quote: ... pCAGGS-SARS2-S-FKO (C-flag) was a gift from Hyeryun Choe & Michael Farzan (Addgene plasmid # 159364 ...
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bioRxiv - Genetics 2022Quote: ... The plenti-CAG-gate-FLAG-IRES-GFP vector was a gift from William Kaelin (Addgene plasmid #107398 ...
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bioRxiv - Cancer Biology 2022Quote: ... the TNFR1 reference sequence from pBMNZ-neo-Flag-TNFR1 L380A (gift from Martin Kluger, Addgene plasmid # 43949 ...
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bioRxiv - Cell Biology 2022Quote: ... Flag-tagged mTOR (Plasmid #26603) and HA-tagged Raptor (Plasmid #8513) were purchased from Addgene. Transfection were performed with the TransIT-X2 Transfection Reagent (Cat ...
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bioRxiv - Neuroscience 2022Quote: The pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931) was a gift from Biff Forbush (Addgene plasmid # 49063 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were co-transfected with enveloping (pCMV14-3X-Flag-SARS-CoV-2 S (Addgene #145780) for PVs or pMD2.G (Addgene #12259 ...
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bioRxiv - Cancer Biology 2023Quote: ... TERT+ cells were transiently transfected with human CA-FOXO1 (pcDNA3 Flag-FKHR-AAA mutant; Addgene) (15) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-APEX2 fragment was amplified form pcDNA3 APEX2-NES (Addgene, 49386 (Lam et al., 2015)) ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-PLK4 cDNA (CDS) was cloned into the lentiviral pCW57-hygro plasmid (Addgene plasmid, 80922) by double digestion with NheI and BamHI and ligation with T4 ligase ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-KD-IKKβ (K44M) (Cat# 11104) and HA-β-TrCP2 (Cat# #36969) were from Addgene. Flag-CA-IKKα (S176E ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids pEGFP-C1-SopF (#137734) and pMRX-INU-FLAG-FAM134B (#128260) were obtained from Addgene. SopF cDNA from pEGFP-C1-SopF was subcloned into FLAG-pcDNA to generate FLAG-SOPF plasmids ...
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bioRxiv - Microbiology 2024Quote: Triple FLAG-tagged Vpr and Vpx lentiviral expression vectors for HIV-2RODVpx (Addgene plasmid #115816), SIVSAB-92018Vpr (Addgene plasmid #115822) ...
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bioRxiv - Immunology 2021Quote: ... The human full length (HFL) gene sequence was subcloned from pcDNA5/FRT/TO HIS HSPA1A (a gift from Harm Kampinga, Addgene plasmid # 19537 ...
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bioRxiv - Molecular Biology 2021Quote: ... and TopBP1 was produced as a fused protein with a GST-tag (GST TopBP1 (aa 32-1522) His from Addgene; plasmid # 20375) ...
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bioRxiv - Molecular Biology 2020Quote: A pET28a vector with sub-cloned cDNA of Hsp53-(1-73) (72R) and the N-terminal His-tag was procured from Addgene, (plasmid #62082) ...
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bioRxiv - Biochemistry 2022Quote: ... with an initial Met and a cleavable C- terminal TEV 6x-His tag was a gift from Ginkgo Bioworks (Addgene plasmid 145611 ...
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bioRxiv - Biochemistry 2022Quote: ... with an initial Met and a cleavable C-terminal TEV 6x-His tag) was a gift from Ginkgo Bioworks (Addgene plasmid 145584 ...
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bioRxiv - Molecular Biology 2022Quote: ... The EcoRI-HpaI fragment of wild-type or mutant Claspin DNA from mAG-TEV-His-Claspin-Flag3 was inserted at the EcoRI/SnaBI site of pMX-IP (Addgene) to construct retroviral expression vectors ...
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bioRxiv - Biochemistry 2021Quote: ... and all described mutants were independently cloned and expressed as a 6× His-SUMO fusion proteins from the expression vector pAL (Addgene). These were cloned utilizing primers in Table S3 ...
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bioRxiv - Biochemistry 2020Quote: FUS LC and FUS LC 12E, soluble His-tag purifications as described (Monahan et al., 2017) (Addgene ID: 98653, 98654)
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bioRxiv - Neuroscience 2020Quote: ... Human His-tagged SETD1A expression pET28-SETD1A-MHL plasmid and its control pET28-MHL were gifts from Cheryl Arrowsmith (Addgene plasmid # 32868 and #26096 ...
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bioRxiv - Microbiology 2020Quote: ... or human ACE2 ectodomain (containing a C-terminal His tag) were made according to the E and F sections of the pLKO.1 Protocol from Addgene (http://www.addgene.org/protocols/plko/) ...
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bioRxiv - Immunology 2021Quote: ... par-5 and his-1 cDNA were subcloned into pCE-BiFC-VN173 and pCE-BiFC-VC155 plasmids (Addgene, Cambridge, MA), which contain the heat shock promoter Phsp-16.41 ...
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bioRxiv - Synthetic Biology 2021Quote: ... or Omphalotin A (OphA) genes with C-terminal His-tags were cloned into the UTR1-T7RNAP-T500 plasmid backbone (Catalog No. 67739, Addgene). The T7 Max promoter was further cloned into these plasmids for downstream experiments ...
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bioRxiv - Cell Biology 2022Quote: ... we added a signal peptide at the N-terminus of Breg and a 6×His tag at its C-terminus using pHL-sec vector (Addgene)39 ...
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bioRxiv - Neuroscience 2022Quote: ... An adeno-associated virus (AAV) solution expressing the CaMPARI2 sensor (hsyn-NES-his-CaMPARI2-WPRE-SV40, Addgene catalog number 101060) was injected into two locations ...
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bioRxiv - Cell Biology 2023Quote: ... Obtained pcDNA-Halo was further digested with HindIII/Bam-HI restriction enzymes and ligated with the NPM insert (obtained by PCR amplification from eGFP-NPM (17578 Addgene) with our primers 5’-CCCAAGCTTCCACCATGGAAGATTCGATGGACATGG-3’ and 5’-CGGGATCCAAGAGACTTCCTCCACTGCC-3’ ...
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bioRxiv - Biophysics 2023Quote: ... The plasmids for his-FUSLC (residue 1 to 163) and LAF-1 RGG (residue 1 to 168) were acquired from Addgene (https://www.addgene.org/127192/ (Fawzi laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding integrin β4 was amplified from pcDNA3.1/Myc-His beta4 (a gift from Filippo Giancotti, Addgene #16039), and then inserted into the pEGFP-N1 vector for the expression of β4-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... the C162A mutant and the C162L mutant were independently cloned and expressed as 6× His-SUMO fusion proteins from the expression vector pAL (Addgene). BL21 (DE3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Pcax Itgb1-FLAG was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid 30153) (8) ...
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bioRxiv - Cell Biology 2019Quote: ... Flag pLJM1 RagB and RagD plasmids were gifts from David Sabatini (Addgene plasmid 19313 and 19316) (Sancak et al. ...
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bioRxiv - Genomics 2019Quote: ... or NIH3T3 cells transiently transfected with Denr cloned into a FLAG-Venus-pLenti6 vector (Addgene 36948). Cells were grown to confluency in a 15-cm dish ...
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bioRxiv - Systems Biology 2021Quote: ... and pAdTrack-CMV FLAG Rev-erbα expression vectors (a gift from Bert Vogelstein; Addgene plasmid #16405) for ectopic expression of Cry1 and Rev-erbα ...
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bioRxiv - Cell Biology 2020Quote: ... GW1-Mito-pHRed (#31474)41 and pcDNA3-FLAG-Rheb (#19996)61 plasmids were purchased from Addgene.
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bioRxiv - Cancer Biology 2021Quote: Cell lines stably expressing Cas9 were generated by lentiviral infection with pCRISPRV2-FLAG-CAS9 (Addgene #52961) followed by puromycin selection and monoclonal population generation by limiting dilution in 96 well plates ...
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bioRxiv - Neuroscience 2021Quote: ... The pCMV14-3X-Flag-SARS-CoV-2 S plasmid was a gift from Zhaohui Qian (Addgene plasmid # 145780 ...
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bioRxiv - Neuroscience 2022Quote: ... A pcDNA3 flag HA 14-3-3 β plasmid was a gift from William Sellers (Addgene plasmid # 8999 ...
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bioRxiv - Molecular Biology 2021Quote: Mouse FLAG-Sp7 or osteocrin cDNAs were introduced via lentivirus via pLX_311 backbone (Addgene, plasmid 118018) as previously described [21] ...
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bioRxiv - Pathology 2021Quote: The constitutively activated STAT3 plasmids (STAT3-C Flag pRc/CMV) were purchased from Addgene (Plasmid #8722). C2C12 cells were transiently transfected with STAT3-C plasmids using the Lipofectamine™ 2000 Transfection Reagent according to the manufacturer’s instructions (Life Technology ...
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bioRxiv - Molecular Biology 2019Quote: ... Flag-Rheb (Q64L)-pcw107 was a gift from David Sabatini and Kris Wood (Addgene plasmid #64607).
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bioRxiv - Immunology 2021Quote: ... Greene) and pCMV14-3X-Flag-SARS-CoV-2 S (a gift from Zhaohui Qian, Addgene #145780) at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-Snail-6SA and GFP-SNAIL-6SA were obtained from Mien-Chie Hung through Addgene (Addgene plasmid # 16225 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-DRD2 was generated by PCR amplifying the DRD2 CDS from DRD2-Tango (Addgene #66269) (introducing a stop codon ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: Murine Taz was expressed by transfecting cells with pEF-TAZ-N-Flag from Michael Yaffe (Addgene #19025 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-HTR2A was generated by PCR amplifying the HTR2A CDS from HTR2A-Tango (Addgene #66409) (introducing a stop codon ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-ADRB2 was generated by PCR amplifying the ADRB2 CDS from ADRB2-Tango (Addgene #66220) (introducing a stop codon ...